High lymphocyte‑to‑monocyte ratio is associated with low α‑fetoprotein expression in patients with hepatitis B virus‑associated hepatocellular carcinoma.

Abstract:

:The association of the peripheral lymphocyte‑to‑monocyte ratio (LMR) with α‑fetoprotein (AFP) status in patients with AFP‑positive and AFP‑negative hepatocellular carcinoma (HCC) has not been investigated in detail. The aim of the present study was to examine the association between the LMR and AFP status in these patients. The samples were obtained from patients with a hepatitis B virus (HBV) infection, who were negative for non‑HBV hepatitis viruses and who did not suffer from autoimmune hepatitis. These patients were retrospectively reviewed and the differences of test indicators in the AFP‑negative and AFP‑positive groups were assessed. Flow cytometry was used to detect the expression levels of CD4, CD8 and programmed cell death protein 1 (PD‑1), and ELISAs were used to analyze the expression levels of interleukin (IL)‑10 and transforming growth factor (TGF)‑β1. In addition, luciferase reporter assays were used to assess binding of the IL‑10 promoter to the glucocorticoid receptor (GR) gene. Receiver operating characteristic curve and Spearman correlation analyses demonstrated that the AFP‑negative HCC group exhibited a higher LMR, lower D‑dimer and lower fibrin degradation products compared with the AFP‑positive HCC group. The cut‑off value of the LMR was 2.01 for AFP detection, with a sensitivity of 68.6% and a specificity of 75%. The high LMR noted in the AFP‑negative HCC group was accompanied by a lower proportion of CD4+ T lymphocytes and CD8‑PD‑1 expression compared with the corresponding levels of these parameters in the AFP‑positive HCC group. Furthermore, the high levels of IL‑10 and low levels of TGF‑β1 were expressed in the AFP‑positive HCC group. The data indicated that the IL‑10‑592 promoter exhibited a potent induction of luciferase activity in 293T cells cotransfected with a GR‑overexpressing vector compared with the control cells. However, the relative luciferase activity was not altered following a mutation or polymorphism in the IL‑10 gene. These results suggested that a high LMR was indicative of low AFP expression in HBV‑associated HCC patients.

journal_name

Mol Med Rep

authors

Wang H,Xiang Y,Li X,Liu S,Liu L

doi

10.3892/mmr.2020.11387

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2673-2684

issue

4

eissn

1791-2997

issn

1791-3004

journal_volume

22

pub_type

杂志文章
  • Correlation between oxidative stress and the NF-κB signaling pathway in the pulmonary tissues of obese asthmatic mice.

    abstract::The obesity-asthma phenotype is characterized by increased asthma severity and decreased glucocorticoid responsiveness. To date, the mechanism underlying the association between obesity and asthma remain to be fully elucidated. The present study investigated the correlation between oxidative stress and the nuclear fac...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4663

    authors: Liu X,Lin R,Zhao B,Guan R,Li T,Jin R

    更新日期:2016-02-01 00:00:00

  • Vanillin improves scopolamine‑induced memory impairment through restoration of ID1 expression in the mouse hippocampus.

    abstract::4-Hydroxy-3-methoxybenzaldehyde (vanillin), contained in a number of species of plant, has been reported to display beneficial effects against brain injuries. In the present study, the impact of vanillin on scopolamine‑induced alterations in cognition and the expression of DNA binding protein inhibitor ID‑1 (ID1), one...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8401

    authors: Lee JC,Kim IH,Cho JH,Lee TK,Park JH,Ahn JH,Shin BN,Yan BC,Kim JD,Jeon YH,Lee YJ,Won MH,Kang IJ

    更新日期:2018-03-01 00:00:00

  • Regulation of Cu-Zn superoxide dismutase on SCN2A in SH-SY5Y cells as a potential therapy for temporal lobe epilepsy.

    abstract::In order to evaluate SCN2A as a candidate gene for epileptic susceptibility and the use of a Cu-Zn superoxide dismutase (SOD) supplement as a potential therapy for epilepsy, SCN2A expression in the cortex and the correlation between SCN2A and Cu-Zn SOD in SH-SY5Y cells were examined. SCN2A expression and the concentra...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1790

    authors: Xiang J,Jiang Y

    更新日期:2014-01-01 00:00:00

  • Flotillin-1 expression in human clear-cell renal cell carcinoma is associated with cancer progression and poor patient survival.

    abstract::The present study was designed to elucidate the expression levels and the proliferative effect of flotillin-1, an integral membrane protein encoded by the FLOT1 gene, in human clear-cell renal cell carcinoma (RCC). Flotillin has been implicated in other types of cancer, but the role of flotillin in RCC has not been es...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2310

    authors: Zhang Y,Li J,Song Y,Chen F,Pei Y,Yao F

    更新日期:2014-08-01 00:00:00

  • Characterization of pancreatic ductal adenocarcinoma using whole transcriptome sequencing and copy number analysis by single-nucleotide polymorphism array.

    abstract::The aim of the current study was to implement whole transcriptome massively parallel sequencing (RNASeq) and copy number analysis to investigate the molecular biology of pancreatic ductal adenocarcinoma (PDAC). Samples from 16 patients with PDAC were collected by ultrasound‑guided biopsy or from surgical specimens for...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4344

    authors: Di Marco M,Astolfi A,Grassi E,Vecchiarelli S,Macchini M,Indio V,Casadei R,Ricci C,D'Ambra M,Taffurelli G,Serra C,Ercolani G,Santini D,D'Errico A,Pinna AD,Minni F,Durante S,Martella LR,Biasco G

    更新日期:2015-11-01 00:00:00

  • [Corrigendum] Pro‑neurogenic effects of andrographolide on RSC96 Schwann cells in vitro.

    abstract::Following the publication of the above article, an interested reader drew to the authors' attention that a pair of the data panels shown in Fig. 4 contained overlapping data. After having consulted their original data, the authors realized that a total of three panels featured in Figs. 4 and 6 had been erroneously sel...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章,已发布勘误

    doi:10.3892/mmr.2020.11278

    authors: Xu F,Wu H,Zhang K,Lv P,Zheng L,Zhao J

    更新日期:2020-09-01 00:00:00

  • Endoplasmic reticulum stress mediates sulforaphane-induced apoptosis of HepG2 human hepatocellular carcinoma cells.

    abstract::Sulforaphane (SFN) is a naturally occurring chemopreventive agent, which effectively inhibits proliferation of HepG2 human hepatocellular carcinoma cells via mitochondria‑mediated apoptosis. Endoplasmic reticulum stress is considered the most important cause of cell apoptosis; therefore, the present study aimed to det...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.6016

    authors: Zou X,Qu Z,Fang Y,Shi X,Ji Y

    更新日期:2017-01-01 00:00:00

  • DNase I aggravates islet β-cell apoptosis in type 2 diabetes.

    abstract::Deoxyribonuclease I (DNase I) is an endonuclease responsible for the destruction of chromatin during apoptosis. However, its role in diabetes remains unclear. The aim of the current study was to investigate the role of DNase I combined with high glucose levels in β‑cell apoptosis. Human samples were collected and the ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5102

    authors: Zhu B,Zhang L,Zhang YY,Wang L,Li XG,Liu T,Fu YK,Zheng YF,Li P,Zhao ZG

    更新日期:2016-06-01 00:00:00

  • Paecilomyces tenuipes extract prevents depression-like behaviors in chronic unpredictable mild stress-induced rat model via modulation of neurotransmitters.

    abstract::The medicinal fungus Paecilomyces tenuipes exhibits a variety of pharmacological effects, including antidepressive effects. The chronic unpredictable mild stress (CUMS)‑induced rat model has served an important role in studies involving antidepressants screening. The aim of the present study was to evaluate the antide...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6807

    authors: Liu C,Wang J,Xu S,An S,Tang S,He J,Liu Y,Lee RJ,Wang D

    更新日期:2017-08-01 00:00:00

  • miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2.

    abstract::To investigate the role of microRNA (miR)‑149 in the cardiac differentiation of mouse bone marrow mesenchymal stem cells (MSCs) in vitro, MSCs were infected with a lentivirus overexpressing miR‑149 and the effect on cardiac differentiation was determined. The quantitative polymerase chain reaction results demonstrated...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8903

    authors: Lu M,Xu L,Wang M,Guo T,Luo F,Su N,Yi S,Chen T

    更新日期:2018-06-01 00:00:00

  • Moscatilin induces apoptosis of pancreatic cancer cells via reactive oxygen species and the JNK/SAPK pathway.

    abstract::Moscatilin is a bibenzyl derivative extracted from the Dendrobium aurantiacum var. denneanum, which has traditionally been used as an immunomodulatory treatment in China. The present study was designed to determine whether moscatilin is a pro‑apoptotic agent in pancreatic cancer, and to elucidate the underlying mechan...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6144

    authors: Zhang L,Fang Y,Xu XF,Jin DY

    更新日期:2017-03-01 00:00:00

  • Flagellin influences the expression of a variety of important cytokines and chemokines without affecting the immune status of umbilical cord mesenchymal stem cells.

    abstract::Multipotent differentiation ability, low expression of co‑stimulatory molecules and immunosuppressive effects render mesenchymal stem cells (MSCs) the best candidate for use in cell‑based therapy. At present, MSCs are widely used in clinical trials, which have, however, failed to demonstrate any long‑term therapeutic ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4276

    authors: Li Q,Ma Y,Li L,Bao J,Zhang L

    更新日期:2015-11-01 00:00:00

  • Analysis of the effect of integrons on drug-resistant Staphylococcus aureus by multiplex PCR detection.

    abstract::The aim of this study was to detect class Ⅰ, II and III integrons using multiplex PCR, and to analyze the role that these integrons play in mediating multidrug-resistant Staphylococcus aureus (SA). The sensitivity of SA to 20 types of antibiotics was examined using the K-B method. A...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1284

    authors: Ren C,Zhao Y,Shen Y

    更新日期:2013-03-01 00:00:00

  • Antitumor effects of Tubeimoside-1 in NCI-H1299 cells are mediated by microRNA-126-5p-induced inactivation of VEGF-A/VEGFR-2/ERK signaling pathway.

    abstract::Tubeimoside-1 (TBMS1), a triterpenoid saponin isolated from the tuber of Bolbostemma paniculatum (Maxim) Franquet, serves an universal suppressive role in multiple cancer types, including lung cancer. However, the mechanism involved in non‑small cell lung cancer (NSCLC) cells by which TBMS1 elicits its antitumor effec...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.8459

    authors: Shi H,Bi H,Sun X,Dong H,Jiang Y,Mu H,Liu G,Kong W,Gao R,Su J

    更新日期:2018-03-01 00:00:00

  • miR‑141 inhibits glioma vasculogenic mimicry by controlling EphA2 expression.

    abstract::Human glioma is a pernicious tumor from the central nervous system; it has been reported that microRNAs (miRs) may have carcinogenic or tumor suppressor effects on human glioma. The aim of the present study was to assess miR‑141 expression and functional role in human primary glioma, as well as in tumor‑derived cell l...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9108

    authors: Li G,Huang M,Cai Y,Ke Y,Yang Y,Sun X

    更新日期:2018-08-01 00:00:00

  • Telmisartan prevents proliferation and promotes apoptosis of human ovarian cancer cells through upregulating PPARγ and downregulating MMP‑9 expression.

    abstract::The mortality rate of ovarian cancer is the highest of all gynecological malignancies. Telmisartan is a commonly used clinical angiotensin receptor blocker, which has antihypertensive, anti‑inflammatory and antithrombotic effects. In the present study, it was investigated whether telmisartan could exert anticancer eff...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4512

    authors: Pu Z,Zhu M,Kong F

    更新日期:2016-01-01 00:00:00

  • Effect of VX‑765 on the transcriptome profile of mice spinal cords with acute injury.

    abstract::Previous studies have shown that caspase-1 plays an important role in the acute inflammatory response of spinal cord injury (SCI). VX‑765, a novel and irreversible caspase‑1 inhibitor, has been reported to effectively intervene in inflammation. However, the effect of VX‑765 on genome‑wide transcription in acutely inju...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11129

    authors: Chen J,Chen YQ,Wang SN,Duan FX,Shi YJ,Ding SQ,Hu JG,Lü HZ

    更新日期:2020-07-01 00:00:00

  • Niclosamide enhances the cytotoxic effect of cisplatin in cisplatin-resistant human lung cancer cells via suppression of lung resistance-related protein and c-myc.

    abstract::Lung cancer is a leading cause of cancer-associated mortality worldwide. The cisplatin (DDP)‑based chemotherapy remains the foundation of treatment for the majority of patients affected by advanced non‑small cell lung cancer (NSCLC). However, DDP‑resistance limits the clinical utility of this drug in patients with adv...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8301

    authors: Zuo Y,Yang D,Yu Y,Xiang M,Li H,Yang J,Li J,Jiang D,Zhou H,Xu Z,Yu Z

    更新日期:2018-03-01 00:00:00

  • Analysis of gene expression profiles associated with glioma progression.

    abstract::The present study aimed to investigate changes at the transcript level that are associated with spontaneous astrocytoma progression, and further, to discover novel targets for glioma diagnosis and therapy. GSE4290 microarray data downloaded from Gene Expression Omnibus were used to identify the differentially expresse...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.3583

    authors: Hu G,Wei B,Wang L,Wang L,Kong D,Jin Y,Sun Z

    更新日期:2015-08-01 00:00:00

  • MicroRNA-100 functions as a tumor suppressor by inhibiting Lgr5 expression in colon cancer cells.

    abstract::Previous studies have demonstrated that microRNAs (miRNAs), a class of single‑stranded RNA molecules that are 18‑27 nucleotides in length, serve a critical function in tumorigenesis, including in the development of colon cancer. In the current study, miR‑100 levels were demonstrated to be reduced in colon cancer tissu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.3052

    authors: Zhou MK,Liu XJ,Zhao ZG,Cheng YM

    更新日期:2015-04-01 00:00:00

  • Kinesin family member 20B regulates tongue cancer progression by promoting cell proliferation.

    abstract::Oral cancer refers to the malignant tumors that occur in the oral cavity, of which 80% are squamous cell carcinomas. The incidence of oral cancer accounts for ~5% of the incidence of systemic malignancies, with rapid progression, extensive infiltration and poor prognosis. In the present study, Kinesin family member (K...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9851

    authors: Li ZY,Wang ZX,Li CC

    更新日期:2019-03-01 00:00:00

  • Synthesis of regioselectively acylated quercetin analogues with improved antiplatelet activity.

    abstract::The aim of the present study was to report on a complete synthetic approach, namely benzylation-hydrolysis-acylation‑hydrogenation, to the synthesis of regioselectively acylated quercetin analogues using low‑cost rutin as a starting material. Three quercetin analogues, quercetin‑3‑O‑propionate (Q‑pr), quercetin‑3‑O‑bu...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7781

    authors: Duan Y,Sun N,Xue M,Wang X,Yang H

    更新日期:2017-12-01 00:00:00

  • MicroRNA-15a inhibition protects against hypoxia/reoxygenation-induced apoptosis of cardiomyocytes by targeting mothers against decapentaplegic homolog 7.

    abstract::Myocardial ischemia/reperfusion (I/R) injury is a major pathological process in coronary heart disease and cardiac surgery, and is associated with aberrant microRNA (miR) expression. Previous studies have demonstrated that inhibition of miR-15a expression may ameliorate I/R‑induced myocardial injury. In the present st...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6466

    authors: Yang Y,Ding S,Xu G,Chen F,Ding F

    更新日期:2017-06-01 00:00:00

  • Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells.

    abstract::Repair of cartilage defects remains a challenge for surgeons, owing to its poor self‑repairing capacity. Cartilage tissue engineering, particularly marrow stem cell‑based cartilage regeneration, provides a promising option for the regeneration of damaged cartilage. Although producing tissue‑engineered cartilage from m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4113

    authors: Zhang X,Xue K,Zhou J,Xu P,Huang H,Liu K

    更新日期:2015-10-01 00:00:00

  • c‑Jun N‑terminal kinase/transforming growth factor‑β/Smad3 pathway: Is it associated with endoplasmic reticulum stress‑mediated renal interstitial fibrosis?

    abstract::The present study investigated the role of the c‑Jun N‑terminal kinase (JNK)/transforming growth factor‑β (TGF‑β)/Smad3 pathway in endoplasmic reticulum stress (ERS)‑mediated renal interstitial fibrosis, which would be beneficial for chronic kidney disease (CKD) therapy. In human renal biopsy tissue, the expression le...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10276

    authors: Liu Y,Shen P,Zhou Y,Tang L,Chai H

    更新日期:2019-07-01 00:00:00

  • Combined kidney‑liver perfusion enhances the proliferation effects of hypothermic perfusion on liver grafts via upregulation of IL‑6/Stat3 signaling.

    abstract::A limited number of studies have revealed that adding kidneys to liver perfusion may maintain an improved physiological balance; however, the underlying mechanism remains to be elucidated. The preset study confirmed the protective role of this new model and investigated the underlying mechanisms. Methods: A total of 1...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10379

    authors: Li J,Jia J,He N,Jiang L,Yu H,Li H,Peng Y,Xie H,Zhou L,Zheng S

    更新日期:2019-08-01 00:00:00

  • Identification of key genes induced by platelet-rich plasma in human dermal papilla cells using bioinformatics methods.

    abstract::Dermal papilla cells (DPCs) are located at the base of hair follicles, and are known to induce hair follicle regeneration. Platelet-rich plasma (PRP) functions in hair follicle regeneration. To investigate the influence of PRP on DPCs, the present study analyzed RNA‑seq data of human hair dermal papilla cells (HHDPCs)...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5988

    authors: Shen H,Cheng H,Chen H,Zhang J

    更新日期:2017-01-01 00:00:00

  • Resveratrol reduces acute lung injury in a LPS‑induced sepsis mouse model via activation of Sirt1.

    abstract::The development of acute lung injury (ALI) during sepsis almost doubles the mortality rate of patients. The efficacy of current treatment strategies is low as treatment is usually initiated following the onset of symptoms. Inflammation is one of the main mechanisms of autoimmune disorders and is a common feature of se...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1444

    authors: Li T,Zhang J,Feng J,Li Q,Wu L,Ye Q,Sun J,Lin Y,Zhang M,Huang R,Cheng J,Cao Y,Xiang G,Zhang J,Wu Q

    更新日期:2013-06-01 00:00:00

  • Fms-related tyrosine kinase 3 ligand promotes proliferation of placenta amnion and chorion mesenchymal stem cells in vitro.

    abstract::Placental mesenchymal stem cells (PMSCs) have important biological properties and the potential for application in numerous clinical fields, including hematopoietic stem cell transplantation and myocardial repair. There are two types of MSCs in the placenta, amniotic mesenchymal stem cells (AMSCs) and chorion mesenchy...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2220

    authors: Li F,Xu Y,Xu X,Xu B,Zhao J,Zhang X

    更新日期:2014-07-01 00:00:00

  • Expression profiles of long noncoding RNAs associated with the NSUN2 gene in HepG2 cells.

    abstract::NOP2/Sun domain family member 2 (NSUN2) is upregulated in numerous types of tumors and may be implicated in multiple biological processes, including cell proliferation, migration and human tumorigenesis. However, little is known about how NSUN2 serves a role in these processes. In the present study, expression profile...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9984

    authors: Sun Z,Xue S,Xu H,Hu X,Chen S,Yang Z,Yang Y,Ouyang J,Cui H

    更新日期:2019-04-01 00:00:00