Abstract：
:Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer organoids from primary patient material enables a range of therapeutic agents to be tested in the resulting organoid cultures. Patient-derived cancer organoids therefore hold great promise for personalized medicine. Here, we provide an overview of the protocols used by different groups to establish organoids from various epithelial tissues and cancers, plus the different protocols subsequently used to test the in vitro therapy sensitivity of these patient-derived organoids. We also provide an in-depth protocol for the generation of head and neck squamous cell carcinoma organoids and their subsequent use in semi-automated therapy screens. Establishment of organoids and subsequent screening can be performed within 3 months, although this timeline is highly dependent on a.o. starting material and the number of therapies tested. The protocol provided may serve as a reference to successfully establish organoids from other cancer types and perform drug screenings thereof.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Driehuis E,Kretzschmar K,Clevers Hdoi
10.1038/s41596-020-0379-4subject
Has Abstractpub_date
2020-10-01 00:00:00pages
3380-3409issue
10eissn
1754-2189issn
1750-2799pii
10.1038/s41596-020-0379-4journal_volume
15pub_type
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