当前位置： SCI文献检索 > Nature Communications期刊下所有文献 > Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation.

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation.

Abstract：

:Gene-targeted animal models that are generated by injecting Cas9 and sgRNAs into zygotes are often accompanied by undesired double-strand break (DSB)-induced byproducts and random biallelic targeting due to uncontrollable Cas9 targeting activity. Here, we establish a parental allele-specific gene-targeting (Past-CRISPR) method, based on the detailed observation that pronuclear transfer-mediated cytoplasmic dilution can effectively terminate Cas9 activity. We apply this method in embryos to efficiently target the given parental alleles of a gene of interest and observed little genomic mosaicism because of the spatiotemporal control of Cas9 activity. This method allows us to rapidly explore the function of individual parent-of-origin effects and to construct animal models with a single genomic change. More importantly, Past-CRISPR could also be used for therapeutic applications or disease model construction.

journal_name

Nat Commun

journal_title

Nature communications

authors

Li Y,Weng Y,Bai D,Jia Y,Liu Y,Zhang Y,Kou X,Zhao Y,Ruan J,Chen J,Yin J,Wang H,Teng X,Wang Z,Liu W,Gao S

doi

10.1038/s41467-020-18391-y

subject

Has Abstract

pub_date

2020-09-14 00:00:00

pages

4593

issue

1

issn

2041-1723

pii

10.1038/s41467-020-18391-y

journal_volume

11

pub_type

杂志文章

相关文献

Nature Communications文献大全
  • Latin Americans show wide-spread Converso ancestry and imprint of local Native ancestry on physical appearance.

    abstract：:Historical records and genetic analyses indicate that Latin Americans trace their ancestry mainly to the intermixing (admixture) of Native Americans, Europeans and Sub-Saharan Africans. Using novel haplotype-based methods, here we infer sub-continental ancestry in over 6,500 Latin Americans and evaluate the impact of ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07748-z

    authors： Chacón-Duque JC,Adhikari K,Fuentes-Guajardo M,Mendoza-Revilla J,Acuña-Alonzo V,Barquera R,Quinto-Sánchez M,Gómez-Valdés J,Everardo Martínez P,Villamil-Ramírez H,Hünemeier T,Ramallo V,Silva de Cerqueira CC,Hurtado M,Villegas V

    更新日期：2018-12-19 00:00:00

  • Author Correction: Quantum nanophotonics with group IV defects in diamond.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature communications

    pub_type： 已发布勘误

    doi：10.1038/s41467-020-14316-x

    authors： Bradac C,Gao W,Forneris J,Trusheim ME,Aharonovich I

    更新日期：2020-01-14 00:00:00

  • Thymoproteasomes produce unique peptide motifs for positive selection of CD8(+) T cells.

    abstract：:Positive selection in the thymus provides low-affinity T-cell receptor (TCR) engagement to support the development of potentially useful self-major histocompatibility complex class I (MHC-I)-restricted T cells. Optimal positive selection of CD8(+) T cells requires cortical thymic epithelial cells that express β5t-cont...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms8484

    authors： Sasaki K,Takada K,Ohte Y,Kondo H,Sorimachi H,Tanaka K,Takahama Y,Murata S

    更新日期：2015-06-23 00:00:00

  • RIP1 autophosphorylation is promoted by mitochondrial ROS and is essential for RIP3 recruitment into necrosome.

    abstract：:Necroptosis is a type of programmed cell death with great significance in many pathological processes. Tumour necrosis factor-α(TNF), a proinflammatory cytokine, is a prototypic trigger of necroptosis. It is known that mitochondrial reactive oxygen species (ROS) promote necroptosis, and that kinase activity of recepto...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms14329

    authors： Zhang Y,Su SS,Zhao S,Yang Z,Zhong CQ,Chen X,Cai Q,Yang ZH,Huang D,Wu R,Han J

    更新日期：2017-02-08 00:00:00

  • Stimulus dependent transformations between synaptic and spiking receptive fields in auditory cortex.

    abstract：:Auditory cortex neurons nonlinearly integrate synaptic inputs from the thalamus and cortex, and generate spiking outputs for simple and complex sounds. Directly comparing synaptic and spiking activity can determine whether this input-output transformation is stimulus-dependent. We employ in vivo whole-cell recordings ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-14835-7

    authors： Kim KX,Atencio CA,Schreiner CE

    更新日期：2020-02-27 00:00:00

  • 5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation.

    abstract：:5-Fluorouracil (5-FU) remains the first-line treatment for colorectal cancer (CRC). Although 5-FU initially de-bulks the tumor mass, recurrence after chemotherapy is the barrier to effective clinical outcomes for CRC patients. Here, we demonstrate that p53 promotes WNT3 transcription, leading to activation of the WNT/...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19173-2

    authors： Cho YH,Ro EJ,Yoon JS,Mizutani T,Kang DW,Park JC,Il Kim T,Clevers H,Choi KY

    更新日期：2020-10-21 00:00:00

  • Transcriptional bursting is intrinsically caused by interplay between RNA polymerases on DNA.

    abstract：:Cell-to-cell variability plays a critical role in cellular responses and decision-making in a population, and transcriptional bursting has been broadly studied by experimental and theoretical approaches as the potential source of cell-to-cell variability. Although molecular mechanisms of transcriptional bursting have ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13788

    authors： Fujita K,Iwaki M,Yanagida T

    更新日期：2016-12-07 00:00:00

  • Author Correction: Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature communications

    pub_type： 已发布勘误

    doi：10.1038/s41467-020-19127-8

    authors： Lebreton F,Lavallard V,Bellofatto K,Bonnet R,Wassmer CH,Perez L,Kalandadze V,Follenzi A,Boulvain M,Kerr-Conte J,Goodman DJ,Bosco D,Berney T,Berishvili E

    更新日期：2020-10-09 00:00:00

  • Crystal structure of a lipin/Pah phosphatidic acid phosphatase.

    abstract：:Lipin/Pah phosphatidic acid phosphatases (PAPs) generate diacylglycerol to regulate triglyceride synthesis and cellular signaling. Inactivating mutations cause rhabdomyolysis, autoinflammatory disease, and aberrant fat storage. Disease-mutations cluster within the conserved N-Lip and C-Lip regions that are separated b...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-15124-z

    authors： Khayyo VI,Hoffmann RM,Wang H,Bell JA,Burke JE,Reue K,Airola MV

    更新日期：2020-03-11 00:00:00

  • Critical length scale controls adhesive wear mechanisms.

    abstract：:The adhesive wear process remains one of the least understood areas of mechanics. While it has long been established that adhesive wear is a direct result of contacting surface asperities, an agreed upon understanding of how contacting asperities lead to wear debris particle has remained elusive. This has restricted a...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11816

    authors： Aghababaei R,Warner DH,Molinari JF

    更新日期：2016-06-06 00:00:00

  • Dynamic control of enhancer activity drives stage-specific gene expression during flower morphogenesis.

    abstract：:Enhancers are critical for developmental stage-specific gene expression, but their dynamic regulation in plants remains poorly understood. Here we compare genome-wide localization of H3K27ac, chromatin accessibility and transcriptomic changes during flower development in Arabidopsis. H3K27ac prevalently marks promoter...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-09513-2

    authors： Yan W,Chen D,Schumacher J,Durantini D,Engelhorn J,Chen M,Carles CC,Kaufmann K

    更新日期：2019-04-12 00:00:00

  • Author Correction: Hypervirulent Listeria monocytogenes clones' adaptation to mammalian gut accounts for their association with dairy products.

    abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title：Nature communications

    pub_type： 已发布勘误

    doi：10.1038/s41467-019-11625-8

    authors： Maury MM,Bracq-Dieye H,Huang L,Vales G,Lavina M,Thouvenot P,Disson O,Leclercq A,Brisse S,Lecuit M

    更新日期：2019-08-06 00:00:00

  • Scaling law for excitons in 2D perovskite quantum wells.

    abstract：:Ruddlesden-Popper halide perovskites are 2D solution-processed quantum wells with a general formula A2A'n-1M n X3n+1, where optoelectronic properties can be tuned by varying the perovskite layer thickness (n-value), and have recently emerged as efficient semiconductors with technologically relevant stability. However,...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-04659-x

    authors： Blancon JC,Stier AV,Tsai H,Nie W,Stoumpos CC,Traoré B,Pedesseau L,Kepenekian M,Katsutani F,Noe GT,Kono J,Tretiak S,Crooker SA,Katan C,Kanatzidis MG,Crochet JJ,Even J,Mohite AD

    更新日期：2018-06-08 00:00:00

  • Integrating an electrically active colloidal quantum dot photodiode with a graphene phototransistor.

    abstract：:The realization of low-cost photodetectors with high sensitivity, high quantum efficiency, high gain and fast photoresponse in the visible and short-wave infrared remains one of the challenges in optoelectronics. Two classes of photodetectors that have been developed are photodiodes and phototransistors, each of them ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms11954

    authors： Nikitskiy I,Goossens S,Kufer D,Lasanta T,Navickaite G,Koppens FH,Konstantatos G

    更新日期：2016-06-17 00:00:00

  • Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics.

    abstract：:Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-α. Here we show that lung vascular permeability is controlled mechanically ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms2774

    authors： Mammoto A,Mammoto T,Kanapathipillai M,Wing Yung C,Jiang E,Jiang A,Lofgren K,Gee EP,Ingber DE

    更新日期：2013-01-01 00:00:00

  • Identification of a subset of immunosuppressive P2RX1-negative neutrophils in pancreatic cancer liver metastasis.

    abstract：:The immunosuppressive microenvironment that is shaped by hepatic metastatic pancreatic ductal adenocarcinoma (PDAC) is essential for tumor cell evasion of immune destruction. Neutrophils are important components of the metastatic tumor microenvironment and exhibit heterogeneity. However, the specific phenotypes, funct...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-20447-y

    authors： Wang X,Hu LP,Qin WT,Yang Q,Chen DY,Li Q,Zhou KX,Huang PQ,Xu CJ,Li J,Yao LL,Wang YH,Tian GA,Yang JY,Yang MW,Liu DJ,Sun YW,Jiang SH,Zhang XL,Zhang ZG

    更新日期：2021-01-08 00:00:00

  • The energy cost of polypeptide knot formation and its folding consequences.

    abstract：:Knots are natural topologies of chains. Yet, little is known about spontaneous knot formation in a polypeptide chain-an event that can potentially impair its folding-and about the effect of a knot on the stability and folding kinetics of a protein. Here we used optical tweezers to show that the free energy cost to for...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-017-01691-1

    authors： Bustamante A,Sotelo-Campos J,Guerra DG,Floor M,Wilson CAM,Bustamante C,Báez M

    更新日期：2017-11-17 00:00:00

  • Biocatalytic nanoparticles for the stabilization of degassed single electron transfer-living radical pickering emulsion polymerizations.

    abstract：:Synthetic polymers are indispensable in many different applications, but there is a growing need for green processes and natural surfactants for emulsion polymerization. The use of solid particles to stabilize Pickering emulsions is a particularly attractive avenue, but oxygen sensitivity has remained a formidable cha...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19407-3

    authors： Moreno A,Sipponen MH

    更新日期：2020-11-05 00:00:00

  • Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression.

    abstract：:Base excision repair (BER) initiated by alkyladenine DNA glycosylase (AAG) is essential for removal of aberrantly methylated DNA bases. Genome instability and accumulation of aberrant bases accompany multiple diseases, including cancer and neurological disorders. While BER is well studied on naked DNA, it remains uncl...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-13394-w

    authors： Montaldo NP,Bordin DL,Brambilla A,Rösinger M,Fordyce Martin SL,Bjørås KØ,Bradamante S,Aas PA,Furrer A,Olsen LC,Kunath N,Otterlei M,Sætrom P,Bjørås M,Samson LD,van Loon B

    更新日期：2019-11-29 00:00:00

  • Quantum capacitance mediated carbon nanotube optomechanics.

    abstract：:Cavity optomechanics allows the characterization of a vibration mode, its cooling and quantum manipulation using electromagnetic fields. Regarding nanomechanical as well as electronic properties, single wall carbon nanotubes are a prototypical experimental system. At cryogenic temperatures, as high quality factor vibr...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-15433-3

    authors： Blien S,Steger P,Hüttner N,Graaf R,Hüttel AK

    更新日期：2020-04-02 00:00:00

  • Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.

    abstract：:Intracellular traffic between compartments of the secretory and endocytic pathways is mediated by vesicle-based carriers. The proteomes of carriers destined for many organelles are ill-defined because the vesicular intermediates are transient, low-abundance and difficult to purify. Here, we combine vesicle relocalisat...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-020-19840-4

    authors： Shin JJH,Crook OM,Borgeaud AC,Cattin-Ortolá J,Peak-Chew SY,Breckels LM,Gillingham AK,Chadwick J,Lilley KS,Munro S

    更新日期：2020-11-25 00:00:00

  • Synthetic strategies of supported atomic clusters for heterogeneous catalysis.

    abstract：:Supported atomic clusters with uniform metal sites and definite low-nuclearity are intermediate states between single-atom catalysts (SACs) and nanoparticles in size. Benefiting from the presence of metal-metal bonds, supported atomic clusters can trigger synergistic effects among every metal atom, which contributes t...

    journal_title：Nature communications

    pub_type： 杂志文章,评审

    doi：10.1038/s41467-020-19571-6

    authors： Rong H,Ji S,Zhang J,Wang D,Li Y

    更新日期：2020-11-18 00:00:00

  • Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors.

    abstract：:The liver parenchyma is composed of hepatocytes and bile duct epithelial cells (BECs). Controversy exists regarding the cellular origin of human liver parenchymal tissue generation during embryonic development, homeostasis or repair. Here we report the existence of a hepatobiliary hybrid progenitor (HHyP) population i...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-11266-x

    authors： Segal JM,Kent D,Wesche DJ,Ng SS,Serra M,Oulès B,Kar G,Emerton G,Blackford SJI,Darmanis S,Miquel R,Luong TV,Yamamoto R,Bonham A,Jassem W,Heaton N,Vigilante A,King A,Sancho R,Teichmann S,Quake SR,Nakauchi H,Rash

    更新日期：2019-07-26 00:00:00

  • Hydrogen spillover through Matryoshka-type (ZIFs@)n-1ZIFs nanocubes.

    abstract：:Hydrogen spillover phenomenon is well-documented in hydrogenation catalysis but still highly disputed in hydrogen storage. Until now, the existence of hydrogen spillover through metal-organic frameworks (MOFs) remains a topic of ongoing debate and how far the split hydrogen atoms diffuse in such materials is unknown. ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-06269-z

    authors： Zhan G,Zeng HC

    更新日期：2018-09-17 00:00:00

  • Ultrabroadband 3D invisibility with fast-light cloaks.

    abstract：:An invisibility cloak should completely hide an object from an observer, ideally across the visible spectrum and for all angles of incidence and polarizations of light, in three dimensions. However, until now, all such devices have been limited to either small bandwidths or have disregarded the phase of the impinging ...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-019-12813-2

    authors： Tsakmakidis KL,Reshef O,Almpanis E,Zouros GP,Mohammadi E,Saadat D,Sohrabi F,Fahimi-Kashani N,Etezadi D,Boyd RW,Altug H

    更新日期：2019-10-24 00:00:00

  • Global perturbation of organic carbon cycling by river damming.

    abstract：:The damming of rivers represents one of the most far-reaching human modifications of the flows of water and associated matter from land to sea. Dam reservoirs are hotspots of sediment accumulation, primary productivity (P) and carbon mineralization (R) along the river continuum. Here we show that for the period 1970-2...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms15347

    authors： Maavara T,Lauerwald R,Regnier P,Van Cappellen P

    更新日期：2017-05-17 00:00:00

  • Social support reduces stress hormone levels in wild chimpanzees across stressful events and everyday affiliations.

    abstract：:Stress is a major cause of poor health and mortality in humans and other social mammals. Close social bonds buffer stress, however much of the underlying physiological mechanism remains unknown. Here, we test two key hypotheses: bond partner effects occur only during stress (social buffering) or generally throughout d...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms13361

    authors： Wittig RM,Crockford C,Weltring A,Langergraber KE,Deschner T,Zuberbühler K

    更新日期：2016-11-01 00:00:00

  • Structure and mechanism of an intramembrane liponucleotide synthetase central for phospholipid biosynthesis.

    abstract：:Phospholipids are elemental building-block molecules for biological membranes. Biosynthesis of phosphatidylinositol, phosphatidylglycerol and phosphatidylserine requires a central liponucleotide intermediate named cytidine-diphosphate diacylglycerol (CDP-DAG). The CDP-DAG synthetase (Cds) is an integral membrane enzym...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms5244

    authors： Liu X,Yin Y,Wu J,Liu Z

    更新日期：2014-06-27 00:00:00

  • The two-fluid dynamics and energetics of the asymmetric magnetic reconnection in laboratory and space plasmas.

    abstract：:Magnetic reconnection is a fundamental process in magnetized plasma where magnetic energy is converted to plasma energy. Despite huge differences in the physical size of the reconnection layer, remarkably similar characteristics are observed in both laboratory and magnetosphere plasmas. Here we present the comparative...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/s41467-018-07680-2

    authors： Yamada M,Chen LJ,Yoo J,Wang S,Fox W,Jara-Almonte J,Ji H,Daughton W,Le A,Burch J,Giles B,Hesse M,Moore T,Torbert R

    更新日期：2018-12-06 00:00:00

  • Rapid changes in the electrical state of the 1999 Izmit earthquake rupture zone.

    abstract：:Crustal fluids exist near fault zones, but their relation to the processes that generate earthquakes, including slow-slip events, is unclear. Fault-zone fluids are characterized by low electrical resistivity. Here we investigate the time-dependent crustal resistivity in the rupture area of the 1999 Mw 7.6 Izmit earthq...

    journal_title：Nature communications

    pub_type： 杂志文章

    doi：10.1038/ncomms3116

    authors： Honkura Y,Oshiman N,Matsushima M,Barış S,Kemal Tunçer M,Bülent Tank S,Celik C,Ciftçi ET

    更新日期：2013-01-01 00:00:00