Abstract:
:Colorectal cancer (CRC) is a common malignancy globally. The aim of the present study was to explore the role and the working mechanism of circular RNA NADPH oxidase 4 (circNOX4; circBase ID, hsa_circ_0023990) in CRC. Reverse transcription‑quantitative (RT‑q)PCR was used to examine the expression of circNOX4, NOX4 mRNA and microRNA (miR)‑485‑5p in CRC tissues and cell lines. 3‑(4,5‑Dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and Transwell assays were performed to analyze CRC cell viability and motility. The glycolytic ability of CRC cells was assessed by measuring glucose consumption, lactate production, extracellular acidification and O2 consumption rates using commercial kits. The starBase database was used to predict the targets of circNOX4 and miR‑485‑5p, and the interaction was confirmed by dual‑luciferase reporter and RNA immunoprecipitation assays. A murine xenograft model was established to verify the role of circNOX4 in CRC in vivo. The results demonstrated that the expression of circNOX4 was upregulated in CRC tissues and cell lines compared with that in adjacent normal tissues and a normal colon epithelial cell line, respectively. The expression of circNOX4 was negatively associated with the prognosis of patients with CRC. CircNOX4 silencing suppressed the proliferation, migration, invasion and glycolysis of CRC cells. miR‑485‑5p was identified as a target of circNOX4. CircNOX4 promoted CRC progression by sponging miR‑485‑5p. miR‑485‑5p was demonstrated to bind to the 3' untranslated region (UTR) of CDC28 protein kinase regulatory subunit 1B (CKS1B). miR‑485‑5p overexpression‑mediated malignant properties of CRC cells were partly reversed by the transfection with the CKS1B overexpression plasmid. CircNOX4 silencing restrained the CRC xenograft growth in vivo. Collectively, the results of the present study demonstrated that circNOX4 may serve an oncogenic role in CRC by promoting the proliferation, migration, invasion and glycolysis of CRC cells via the miR‑485‑5p/CKS1B axis.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Wang X,Tao G,Huang D,Liang S,Zheng Ddoi
10.3892/or.2020.7758subject
Has Abstractpub_date
2020-11-01 00:00:00pages
2009-2020issue
5eissn
1021-335Xissn
1791-2431journal_volume
44pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Long non‑coding RNAs (lncRNAs) can act as carcinogenic or cancer suppressive factors during the pathogenesis, invasion and metastasis of non‑small cell lung cancer (NSCLC). The current study explored the role of long intergenic non‑protein coding RNA 00887 (LINC00887) and competing endogenous RNAs (ceRNAs). It was rev...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7228
更新日期:2019-09-01 00:00:00
abstract::The present study aimed to explore the relationship between the efficacy of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKIs) and the expression of the cancer stem cell (CSC)‑related markers. Specimens of 72 cases of lung adenocarcinoma with significantly different therapeutic effects of the firs...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7454
更新日期:2020-02-01 00:00:00
abstract::The signal transducer and activator of transcription 3 (STAT3) are ideal targets for ovarian cancer. Previous studies showed that downregulation of STAT3 using specific short hairpin RNAs (shRNA) can significantly reduce ovarian tumor growth. However, RNA interference does not fully ablate target gene expression due t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3623
更新日期:2015-02-01 00:00:00
abstract::Aberrant methylation of 5' CpG islands is thought to play an important role in the inactivation of tumor suppressor genes in several types of cancers. In non-small cell lung cancer (NSCLC), several genes are known to be frequently methylated and the correlation of their methylation with clinical features has been stud...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::The aim of this study was to assess the ability of a new magnetic resonance (MR) angiography technique that does not use contrast enhancement medium to depict the vascularity of musculoskeletal neoplasms, and evaluate its clinical utility. We performed 3D fresh blood imaging (FBI) MR angiography in 57 patients with bo...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract::In a screen for thoracic malignancy-associated markers, thyroid stimulating hormone receptor (TSHR) was identified as a candidate as it binds to the previously-characterized lung cancer marker NKX2-1. We screened for mutations in all coding regions of the TSHR gene in 96 lung adenoc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1938
更新日期:2012-10-01 00:00:00
abstract::The impact of interferon-γ (IFN-γ) late preconditioning on myocardial ischemia-reperfusion injury during cardiopulmonary bypass (CPB) and the underlying mechanism were investigated. Using a porcine model of myocardial ischemia-reperfusion injury during CPB with a 60-min aorta cross-clamp, 20 pigs (15±0.5 kg) were trea...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2707
更新日期:2013-11-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases, capable of degrading all the molecular components of extracellular matrix. MMPs have been shown to play critical roles in tumor cell invasion and metastasis. We verified the activity of MMPs in the sera ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1864
更新日期:2012-09-01 00:00:00
abstract::Prognostic factors and the role of post-operative radiotherapy (PORT) in patients with pN1 nodal stage following surgery for NSCLC were identified. The clinical course of 211 patients with pN1 nodal involvement following thoracic surgery were reviewed, 97 of them received PORT. Multi-variate survival analysis with res...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a leading cause of cancer‑related morbidity and mortality globally. Despite the remarkable improvements in comprehensive HCC treatment, the underlying mechanistic details of HCC remain elusive. We screened HCC patients for differentially expressed genes (DEGs) using the Gene Expressio...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7779
更新日期:2020-11-01 00:00:00
abstract::Lack of effective therapy is a major problem in the treatment of pancreatic cancer. In the present study, we investigated a natural product, the extract of Pao Pereira (Pao), for its anti-pancreatic cancer effect in vitro and in vivo, either alone or in combination with the first-line chemotherapeutic drug gemcitabine...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2461
更新日期:2013-07-01 00:00:00
abstract::The restricted expression of Wilms tumor 1 (WT1) and cyclin A1 (CCNA1) in normal tissues, as opposed to their abnormal expression in leukemia demonstrates the applicability of WT1 and CCNA1 as cancer antigens for immunotherapy, and as markers for prognosis and relapse. In this study, the WT1 and CCNA1 mRNA levels were...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7287
更新日期:2019-11-01 00:00:00
abstract::Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate and regulate immune responses to various antigens. DC-based vaccines have become a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is expressed at high levels in lung cancer and many other tumor cells, suggesting...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4231
更新日期:2015-11-01 00:00:00
abstract::The novel gene human Biot2 (hBiot2) was first reported by our laboratory. Previously, we indicated its function of proliferation and carcinogenesis in human endometrial cancer. In the present study, we aimed to investigate whether hBiot2 played a similar role in human cervical cancer. We tested hBiot2 expression profi...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Trans-3,4',5-trihydroxystilbene (resveratrol) is a grape polyphenol present in various plants, food products, red wine and grapes. Resveratrol has anti-inflammatory, anticarcinogenic, anti-oxidant and anti-aging properties. Matrix metalloproteinases (MMPs) are key enzymes involved i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2151
更新日期:2013-02-01 00:00:00
abstract::Investigations into the cellular processing of radiolabeled monoclonal antibodies (mAbs) for their further use in radioimmunodiagnosis and cancer therapy are needed in order to understand the fate of internalized and catabolized mAbs. The anti-colorectal cancer mAb, A33, was labelled with 76Br and 125I using the direc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Long non-coding RNA growth arrest-specific 5 (GAS5) was reported to be aberrantly expressed in various types of cancers. However, the role of GAS5 in the evolution and progression of ovarian cancer remains elusive. In the present study, we aimed to investigate the cellular function and clinical significance of GAS5 in...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5200
更新日期:2016-12-01 00:00:00
abstract::To ascertain the implications of loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) for carcinogenesis, the precise frequency of LOI in colorectal carcinoma was examined using a laser capture microdissection method, and compared to the matched normal colorectal mucosa. LOI was examined by PCR-re...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::Nuclear factor (NF)-κB is the key transcription factor involved in the inflammatory responses, and its activation aggravates tumors. Peptidoglycan (PGN), a main cell wall component of Gram-positive bacteria, stimulates Toll-like receptor 2 (TLR-2) and activates a number of inflammat...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1937
更新日期:2012-10-01 00:00:00
abstract::microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity pro...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2452
更新日期:2013-07-01 00:00:00
abstract::Esophageal cancer is one of the most aggressive cancers in the world. Novel preventive and therapeutic strategies tend to target the key molecules involved in the signaling transduction pathways for cell growth. It is known that FAK and mTOR are important controllers of cell growth. TAE226, a novel small molecule comp...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-12-01 00:00:00
abstract::Extracellular matrix remodeling crucial to tumorigenesis involves proteolytic enzymes, primarily matrix metalloproteinases (MMPs). MMP production is stimulated by multiple factors, including the extracellular matrix metallo-proteinase inducer EMMPRIN/CD147. Overexpression of EMMPRIN...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1474
更新日期:2012-01-01 00:00:00
abstract::Pentoxifylline (PTX) is commonly used in peripheral blood vessel diseases, however it has also been found to decrease the level of proinflammatory cytokines such as IL-12, TNF-alpha and IFN-gamma. Moreover, some authors reported that PTX suppresses spontaneous cytotoxicity of peripheral blood mononuclear cells (PBMC) ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.9.2.423
更新日期:2002-03-01 00:00:00
abstract::MicroRNAs (miRNAs) play crucial roles in the initiation and progression of various cancers, including melanoma. Recently, the genetic variants and deregulation of miR-605 have been reported to participate in carcinogenesis. However, the expression status of the miR-605 in melanoma tissues and its role in melanoma prog...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5740
更新日期:2017-08-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the most common tumors in the world. At present, the details of the mechanism responsible for HCC formation and maintenance remain unclear. However, the cancer stem cell (CSC) theory suggests that liver cancer stem cells (LCSCs) may be respon...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2012.2082
更新日期:2013-01-01 00:00:00
abstract::Angiogenesis is a key factor in the growth and dissemination of malignant diseases, including breast cancer, with significant implications for its clinical management. It is known that microRNAs (miRNAs) play important roles in regulating tumor properties in cancers. However, whether miR-497 contributes to breast canc...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4529
更新日期:2016-03-01 00:00:00
abstract::Despite significant homology between IRS-1 and IRS-2, recent studies have revealed distinct functions for these adaptor proteins in regulating breast cancer progression. Thus far, most of the studies on breast cancer have focused upon IRS-1, the biological pattern of IRS-2 is limited. We demonstrated that depletion of...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00001016
更新日期:2010-12-01 00:00:00
abstract::Metastasis is the main cause of death in osteosarcoma. Targeting the process of metastasis is a main strategy for osteosarcoma therapy. As a traditional Chinese medicine, Zanthoxylum nitidum (Roxb) has been applied to treat various diseases, including cancer. However, no evidence has been shown on the anti-metastasis ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4846
更新日期:2016-08-01 00:00:00
abstract::The aim of the study was to compare cross sections with longitudinal sections in histopathological examination of the rat heart after irradiation, to find the most optimal method for the detection of cardiac radiation injuries. For this purpose, rats were irradiated locally on the heart with a single dose of 0 or 20 G...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.12.2.213
更新日期:2004-08-01 00:00:00
abstract::The protein p27/kip1 is a cyclin-dependent kinase inhibitor that regulates cell-cycle progression. In the present study, p27/kip1 expression as well as tumor cell proliferation and apoptosis were investigated in 80 colorectal carcinomas, using anti-p27/kip1 antibodies, in situ apoptosis detection kits and anti-PCNA an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00