Abstract:
:In this study, we propose a novel sensitive solid-based immunosensor developed on a plasmonic nanopaper platform for the detection of Escherichia coli (E. coli) bacteria. This plasmonic nanopaper that comprises of carboxylated bacterial cellulose (CBC) impregnated with gold nanoparticles (AuNP-CBC), employed as a quencher and a sustainable functionalized platform to be conjugated with protein A. Thus, the conjugated protein A allows the aligned linkage of EAb-QD (anti-E. coli conjugated quantum dot) and EAb-AF (anti-E. coli conjugated Alexa Fluor 488). Interestingly, once E. coli was captured by the AuNP-CBC/EAb-QD or AuNP-CBC/EAb-AF, the energy transfer from the QD or Alexa Fluor fluorophores is triggered due to the conformational change in the antibody structure and this, in turn, causes a decrease in the distance between fluorophores and the quencher nanopaper and, therefore diminishing their photoluminescence. The immunosensors performed successfully to recognize E. coli at concentrations as low as 50 CFU mL-1 in the standard buffer. The examined functionality of the immunosensors in a real matrix such as chicken extract and lettuce juice demonstrated a highly efficient response while QD is the main fluorophore with a limit of detection around 100 CFU mL-1.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Heli B,Ajji Adoi
10.1038/s41598-020-71285-3subject
Has Abstractpub_date
2020-09-01 00:00:00pages
14367issue
1issn
2045-2322pii
10.1038/s41598-020-71285-3journal_volume
10pub_type
杂志文章abstract::The P2X receptor family of ATP-gated cation channels are attractive drug targets for pain and inflammatory disease, but no subtype-selective agonists, and few partially selective agonists have been described to date. As proof-of-concept for the discovery of novel P2X receptor agonists, here we demonstrate the use of D...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65169-9
更新日期:2020-05-19 00:00:00
abstract::We propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation fu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43840
更新日期:2017-03-02 00:00:00
abstract::In this letter, making use of real gait force profiles of healthy and patient groups with Parkinson disease which have different disease severity in terms of Hoehn-Yahr stage, we calculate various heuristic complexity measures of the recurrence quantification analysis (RQA). Using this technique, we are able to evince...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27369-2
更新日期:2018-06-14 00:00:00
abstract::First identified in The Netherlands in 2004, human coronavirus NL63 (HCoV-NL63) was found to cause worldwide infections. Patients infected by HCoV-NL63 are typically young children with upper and lower respiratory tract infection, presenting with symptoms including croup, bronchiolitis, and pneumonia. Unfortunately, t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22677
更新日期:2016-03-07 00:00:00
abstract::Hippocampal volumes are smaller in psychiatric disorder patients and lower levels of hippocampal neurogenesis are the hypothesized cause. Understanding which molecular processes regulate hippocampal progenitor differentiation might aid in the identification of novel drug targets that can promote larger hippocampal vol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15042-z
更新日期:2017-11-08 00:00:00
abstract::Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10627-0
更新日期:2017-09-04 00:00:00
abstract::Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCBs), from mouse and human plurip...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41840
更新日期:2017-02-06 00:00:00
abstract::The DNA methyltransferase DNMT3A R882H mutation is observed in 25% of all AML patients. DNMT3A is active as tetramer and the R882H mutation is located in one of the subunit/subunit interfaces. Previous work has reported that formation of mixed wildtype/R882H complexes leads to a strong loss of catalytic activity obser...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31635-8
更新日期:2018-09-05 00:00:00
abstract::This study analyses spatially resolved estimates of mass budget and surface velocity of glaciers in the Zanskar Basin of Western Himalaya in the context of varying debris cover, glacier hypsometry and orientation. The regional glacier mass budget for the period of 1999-2014 is -0.38 ± 0.09 m w.e./a. Individual mass bu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27014-y
更新日期:2018-06-11 00:00:00
abstract::The effects of antithrombotic drugs on random and free flap survival have been investigated in the past, but the experimental and clinical results are not in agreement. A perforator-based critical ischaemia model was used to evaluate the effects of different perioperatively administered pharmaceutical agents on tissue...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35833
更新日期:2016-10-21 00:00:00
abstract::Recent evidence points at the role that human endogenous retroviruses (HERVs) may play through the activation of genes integrated across the human genome. Although a variety of genetic/epigenetic mechanisms maintain most HERVs silenced, independent environmental stimuli including infections may transactivate endogenou...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42788-5
更新日期:2019-04-18 00:00:00
abstract::The replication-incompetent adenovirus (Ad) vector is one of the most promising vectors for gene therapy; however, systemic administration of Ad vectors results in severe hepatotoxicities, partly due to the leaky expression of Ad genes in the liver. Here we show that nuclear factor-kappa B (NF-κB) mediates the leaky e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19922
更新日期:2016-01-27 00:00:00
abstract::The coupling of the surface plasmon near-field into the sensing medium is key to the sensitivity of surface plasmon-based sensing devices. A low-index dielectric is necessary for the sensing medium to support a highly-penetrating surface plasmon evanescent field that extends well into the dielectric medium. The air-li...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38859-2
更新日期:2019-02-19 00:00:00
abstract::The spectral properties of Fano resonance generated in multilayer dielectric gratings (MDGs) are reported and numerically investigated in this paper. We examine the MDG consisting of numerous identically alternative chalcogenide glass (As2S3) and silica (SiO2) multilayers with several grating widths inscribed through ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34787-9
更新日期:2018-11-06 00:00:00
abstract::The melanin-concentrating hormone (MCH) is a peptidergic neuromodulator synthesized by neurons in the lateral hypothalamus and zona incerta. MCHergic neurons project throughout the central nervous system, indicating the involvements of many physiological functions, but the role in pain has yet to be determined. In thi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19145-z
更新日期:2018-01-15 00:00:00
abstract::Eight advanced durum-breeding lines were treated with 5-methyl-azacytidine to test the feasibility of generating sources of Fusarium head blight (FHB) resistance. Of the 800 treated seeds, 415 germinated and were advanced up to four (M4) generations by selfing. Thirty-two of the resulting 415 M4 lines were selected fo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73521-2
更新日期:2020-10-19 00:00:00
abstract::Diabetes is a globally prevalent disease that can cause visible microvascular complications such as diabetic retinopathy and macular edema in the human eye retina, the images of which are today used for manual disease screening and diagnosis. This labor-intensive task could greatly benefit from automatic detection usi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47181-w
更新日期:2019-07-24 00:00:00
abstract::The ubiquitously expressed aryl hydrocarbon receptor (AhR) induces drug metabolizing enzymes as well as regulators of cell growth, differentiation and apoptosis. Certain AhR ligands promote atherosclerosis, an age-associated vascular disease. Therefore, we investigated the role of AhR in vascular functionality and agi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19618
更新日期:2016-01-21 00:00:00
abstract::Manual anthropometrics are used extensively in medical practice and epidemiological studies to assess an individual's health. However, traditional techniques reduce the complicated shape of human bodies to a series of simple size measurements and derived health indices, such as the body mass index (BMI), the waist-hip...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69099-4
更新日期:2020-07-22 00:00:00
abstract::Large-scale studies have not been conducted to assess whether serum hypobicarbonatemia increases the risk for kidney function deterioration and mortality among East-Asians. We aimed to determine the association between serum total CO2 (TCO2) concentrations measured at the first outpatient visit and clinical outcomes. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81332-2
更新日期:2021-01-18 00:00:00
abstract::Insects harbor a wide variety of microorganisms that form complex and changing communities and play an important role in the biology and evolution of their hosts. Aphids have been used as model organisms to study microorganism-insect interactions. Almost all aphids are infected with the obligate endosymbiont Buchnera ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42232-8
更新日期:2019-04-08 00:00:00
abstract::The preparation of mesoporous iron oxides with controllable physiochemical properties for effective therapeutic drug delivery remains a formidable challenge. Herein, iron oxide mesoporous magnetic microparticles (IO-MMMs) were prepared by a modified reverse hard-templating approach using, for the first time, acid-prep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46007-z
更新日期:2019-07-01 00:00:00
abstract::Bacterial microcompartments (BMCs) are promising natural protein structures for applications that require the segregation of certain metabolic functions or molecular species in a defined microenvironment. To understand how endogenous cargos are packaged inside the protein shell is key for using BMCs as nano-scale reac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33074-x
更新日期:2018-10-10 00:00:00
abstract::We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24622-6
更新日期:2018-04-27 00:00:00
abstract::The submarine volcano Axial Seamount has exhibited an inflation predictable eruption cycle, which allowed for the successful forecast of its 2015 eruption. However, the exact triggering mechanism of its eruptions remains ambiguous. The inflation predictable eruption pattern suggests a magma reservoir pressure threshol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67043-0
更新日期:2020-06-23 00:00:00
abstract::Neuromuscular impairment requires adherence to a rehabilitation regimen for maximum recovery of motor function. Consumer-grade game controllers have emerged as a viable means to relay supervised physical therapy to patients' homes, thereby increasing their accessibility to healthcare. These controllers allow patients ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59927-y
更新日期:2020-02-28 00:00:00
abstract::Gyroviruses (GyVs) are small, single-stranded, circular DNA viruses in the genus Gyrovirus, which consists of the chicken anemia virus (CAV) prototype and nine other viral species. These different GyV species have been reported in chickens, humans, mice, and companion animals. To date, CAV has been identified in the f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49955-8
更新日期:2019-09-16 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-53160-y
更新日期:2019-11-05 00:00:00
abstract::Immune checkpoint inhibitors (ICIs) elicit antitumour effects by activating the host immunity and cause immune-related adverse events (irAEs). ICI-related interstitial lung disease (ICI-ILD) is a fatal irAE that is difficult to treat; moreover, its incidence is relatively higher in patients with lung cancer. Therefore...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70743-2
更新日期:2020-08-13 00:00:00
abstract::Photomechanical nanocomposites embedded with light-absorbing nanoparticles show promising applications in photoresponsive actuations. Near infrared (nIR)-responsive nanocomposites based photomechanical soft actuators can offer lightweight functional and underexploited entry into soft robotics, active optics, drug deli...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27366
更新日期:2016-06-06 00:00:00