Characterization of a phosphotyrosyl phosphatase activator homologue of the parasitic nematode Haemonchus contortus and its immunomodulatory effect on goat peripheral blood mononuclear cells in vitro.


:Suppression and modulation of the host immune response to parasitic nematodes have been extensively studied. In the present study, we cloned and produced recombinant phosphotyrosyl phosphatase activator protein from Haemonchus contortus (rHCPTPA), a parasitic nematode of small ruminants, and studied the effect of this protein on modulating the immune response of goat peripheral blood mononuclear cells. Enzymatic assays revealed that rHCPTPA enhanced the p-nitrophenylphosphate phosphatase activity of bovine PP2A1. Immunohistochemical tests verified that the HCPTPA protein was localised mainly in the bowel wall and on the body surface of worms. It was also shown that serum produced by goats artificially infected with H. contortus successfully recognised rHCPTPA, which conjugated with goat peripheral blood mononuclear cells. The rHCPTPA was then co-incubated with goat peripheral blood mononuclear cells to assess its immunomodulatory effects on proliferation, apoptosis, cytokine secretion, migration and nitric oxide production. Our results showed that rHCPTPA suppressed the proliferation of goat peripheral blood mononuclear cells stimulated by concanavalin A and induced apoptosis in goat peripheral blood mononuclear cells. After rHCPTPA exposure, IFN-γ and IL-2 expression was markedly reduced, whereas secretion of IL-10 and IL-4 was significantly elevated, in goat peripheral blood mononuclear cells. Moreover, rHCPTPA down-regulated nitric oxide production and migration of goat peripheral blood mononuclear cells in a dose-dependent manner. These results illuminate the interaction between parasites and hosts at the molecular level, suggest a possible immunomodulatory target and contribute to the search for innovative proteins that might be candidate targets for drugs and vaccines.


Int J Parasitol


Wang Y,Wang W,Ehsan M,Zhang Y,Yan R,Song X,Xu L,Zhang X,Li X




Has Abstract


2020-12-01 00:00:00














  • Diversity and specificity in Diplostomum spp. metacercariae in freshwater fishes revealed by cytochrome c oxidase I and internal transcribed spacer sequences.

    abstract::In this study, sequences from the barcode region of cytochrome c oxidase I (COI) were used to distinguish Diplostomum spp. in a sample of 497 metacercariae collected from diverse fishes of the St. Lawrence River, Canada and findings were corroborated with internal transcribed spacer (ITS) regions of rDNA. Twelve speci...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Locke SA,McLaughlin JD,Dayanandan S,Marcogliese DJ

    更新日期:2010-03-01 00:00:00

  • Reappraisal of Hydatigera taeniaeformis (Batsch, 1786) (Cestoda: Taeniidae) sensu lato with description of Hydatigera kamiyai n. sp.

    abstract::The common cat tapeworm Hydatigera taeniaeformis is a complex of three morphologically cryptic entities, which can be differentiated genetically. To clarify the biogeography and the host spectrum of the cryptic lineages, 150 specimens of H. taeniaeformis in various definitive and intermediate hosts from Eurasia, Afric...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Lavikainen A,Iwaki T,Haukisalmi V,Konyaev SV,Casiraghi M,Dokuchaev NE,Galimberti A,Halajian A,Henttonen H,Ichikawa-Seki M,Itagaki T,Krivopalov AV,Meri S,Morand S,Näreaho A,Olsson GE,Ribas A,Terefe Y,Nakao M

    更新日期:2016-05-01 00:00:00

  • The unhealthy attraction of Plasmodium vivax to reticulocytes expressing transferrin receptor 1 (CD71).

    abstract::The majority of malaria parasite species prefer to invade reticulocytes, the most infamous being Plasmodium vivax. While the absence of an in vitro continuous culture method has hampered the study of P. vivax invasion biology, studies utilising primate models and ex vivo assays have provided some important insights. M...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Malleret B,Rénia L,Russell B

    更新日期:2017-06-01 00:00:00

  • Cytoadherence, pathogenesis and the infected red cell surface in Plasmodium falciparum.

    abstract::The particular virulence of Plasmodium falciparum compared with the other malaria species which naturally infect humans is thought to be due to the way in which the parasite modifies the surface of the infected red cell. Approximately 16 hours into the asexual cycle, parasite encoded proteins appear on the red cell su...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Newbold C,Craig A,Kyes S,Rowe A,Fernandez-Reyes D,Fagan T

    更新日期:1999-06-01 00:00:00

  • Enhanced neutrophil functions during Opisthorchis viverrini infections and correlation with advanced periductal fibrosis.

    abstract::Millions of people are infected with the liver fluke, Opisthorchis viverrini (OV), but only ~25% of those infected develop liver disease and even fewer develop cholangiocarcinoma. The reasons for these differential outcomes following infection are unknown but it has been proposed that differential immune responses to ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Salao K,Spofford EM,Price C,Mairiang E,Suttiprapa S,Wright HL,Sripa B,Edwards SW

    更新日期:2020-02-01 00:00:00

  • A molecular systematic framework for equine strongyles based on ribosomal DNA sequence data.

    abstract::In this study, molecular data sets were used to address the controversies relating to the systematics of strongyloid nematodes of equids utilising morphological data sets. DNA sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of ribosomal DNA were determined for 30 species of equine stro...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Hung GC,Chilton NB,Beveridge I,Gasser RB

    更新日期:2000-01-01 00:00:00

  • Altered expression, polymerisation and cellular distribution of alpha-/beta-tubulins and apoptosis-like cell death in arsenite resistant Leishmania donovani promastigotes.

    abstract::Studies in mammalian systems have shown specific affinity of arsenite for tubulin proteins. The sodium m-arsenite (NaAsO2) resistant Leishmania donovani used in this study is resistant to 20 microM NaAsO2, which is a 13-fold increase in resistance compared to the wild type. Data presented in this study shows decreased...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Jayanarayan KG,Dey CS

    更新日期:2004-07-01 00:00:00

  • A new phylogeny and environmental DNA insight into paramyxids: an increasingly important but enigmatic clade of protistan parasites of marine invertebrates.

    abstract::Paramyxida is an order of rhizarian protists that parasitise marine molluscs, annelids and crustaceans. They include notifiable pathogens (Marteilia spp.) of bivalves and other taxa of economic significance for shellfish production. The diversity of paramyxids is poorly known, particularly outside of commercially impo...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Ward GM,Bennett M,Bateman K,Stentiford GD,Kerr R,Feist SW,Williams ST,Berney C,Bass D

    更新日期:2016-09-01 00:00:00

  • Cloning and characterisation of a highly immunoreactive 37 kDa antigen with multi-immunoglobulin domains from the swine roundworm Ascaris suum.

    abstract::Antigens from larval stages of Ascaris suum have been shown to induce protection against challenge infection with infective A. suum eggs. We previously identified several antigens that reacted strongly with serum from pigs inoculated with infective eggs containing L3. In this study, we isolated an antigen with a molec...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Tsuji N,Kasuga-Aoki H,Isobe T,Arakawa T,Matsumoto Y

    更新日期:2002-12-19 00:00:00

  • Antibody responses against natural Taenia hydatigena infection in dogs in Kenya.

    abstract::Antibody responses (IgG) against Taenia hydatigena infection in dogs in Kenya were analysed in ELISA using excretory/secretory products of T. hydatigena scoleces derived from goat cysticercus cysts. Helminth infections of individual dogs were confirmed at autopsy. T. hydatigena worms were found in 89.5% of 143 dogs, a...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Jenkins DJ,Gasser RB,Romig T,Zeyhle E

    更新日期:1991-04-01 00:00:00

  • Protein disulphide isomerase of Ostertagia ostertagi: an excretory-secretory product of L4 and adult worms?

    abstract::A pepstatin A-agarose column was used in an attempt to purify a previously described antibody-degrading aspartyl proteinase from excretory-secretory material from the L4 and the adult stages of the bovine abomasal nematode Ostertagia ostertagi. However, no aspartyl proteinase activity was detected in the eluted fracti...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Geldhof P,Vercauteren I,Knox D,Demaere V,Van Zeveren A,Berx G,Vercruysse J,Claerebout E

    更新日期:2003-02-01 00:00:00

  • Babesia bovis: biosynthesis and localisation of 12D3 antigen in bovine erythrocytes.

    abstract::The 12D3 antigen of Babesia bovis was found to be synthesised rapidly in cultured parasites, and localised to both the apical complex of the merozoite and the cytoplasm of the parasitised erythrocyte. Amino-terminal sequencing suggested that the nascent protein had been processed and differences between the predicted ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Harper GS,Hibbs AR,East IJ,Waltisbuhl DJ,Jorgensen WK,Riddles PW

    更新日期:1996-11-01 00:00:00

  • Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor.

    abstract::We describe methods for the large-scale in vitro culturing of synchronous and asynchronous blood-stage Plasmodium falciparum parasites in sterile disposable plastic bioreactors controlled by wave-induced motion (wave bioreactor). These cultures perform better than static flask cultures in terms of preserving parasite ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Dalton JP,Demanga CG,Reiling SJ,Wunderlich J,Eng JW,Rohrbach P

    更新日期:2012-01-01 00:00:00

  • A new species of Trichodina (Ciliophora: Peritrichia) from the intestine of the surgeonfish Acanthurus xanthopterus.

    abstract::A population of a trichodinid (Ciliophora: Peritrichia) was found in the gut of the surgeonfish Acanthurus xanthopterus collected from Hawaii, South Africa and New Guinea and described as a new species. This is only the second record of a trichodinid from the intestine of a marine fish. In all the fish specimens exami...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Basson L,Van As JG,Fishelson L

    更新日期:1990-10-01 00:00:00

  • Host specificity dynamics: observations on gyrodactylid monogeneans.

    abstract::The directly transmitted viviparous gyrodactylids have high species richness but low morphological and biological diversity, and many species are recorded from only a single host. They therefore constitute a guild of species ideal for studies of the evolutionary significance of host specificity. The group has the wide...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Bakke TA,Harris PD,Cable J

    更新日期:2002-03-01 00:00:00

  • Demonstration of a range of inflammatory mediators released in trichostrongylosis of sheep.

    abstract::The levels of inflammatory mediators in the intestinal contents of sheep immunized with Trichostrongylus colubriformis larvae increased in the first 6 days after challenge. These mediators were histamine, leukotriene C4, 6-keto-prostaglandin F1 alpha (from prostacyclin) and thromboxane B2. Leukotriene C4 was released ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Jones WO,Emery DL

    更新日期:1991-06-01 00:00:00

  • Trickle or clumped infection process? An analysis of aggregation in the weights of the parasitic roundworm of humans, Ascaris lumbricoides.

    abstract::Studying the distribution of parasitic helminth body size across a population of definitive hosts can advance our understanding of parasite population biology. Body size is typically correlated with egg production. Consequently, inequalities in body size have been frequently measured to infer variation in reproductive...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Walker M,Hall A,Basáñez MG

    更新日期:2010-10-01 00:00:00

  • Pathogenicity of the protozoan parasite Marteilioides chungmuensis in the Pacific oyster Crassostrea gigas.

    abstract::Marteilioides chungmuensis is an ovarian parasite that causes nodule-like structures to appear on the gonads of female Pacific oysters, Crassostrea gigas. It is known that the prevalence of infection increases in summer and decreases from autumn to spring. To investigate the decrease in prevalence of infection and pat...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Tun KL,Itoh N,Shimizu Y,Yamanoi H,Yoshinaga T,Ogawa K

    更新日期:2008-02-01 00:00:00

  • Detection and identification of Trypanosoma of African livestock through a single PCR based on internal transcribed spacer 1 of rDNA.

    abstract::Primers hybridising with the rDNA cistron have previously been evaluated for PCR diagnosis specific for kinetoplastids, and shown to detect and differentiate the Trypanosoma brucei complex and Trypanosoma cruzi. Kin1 and Kin2 primers, amplifying internal transcribed spacer 1, were subsequently evaluated for the diagno...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Desquesnes M,McLaughlin G,Zoungrana A,Dávila AM

    更新日期:2001-05-01 00:00:00

  • Toxicity of cadmium and zinc to Diplostomum spathaceum (Trematoda: Diplostomidae) cercarial survival.

    abstract::The toxicity of cadmium and zinc at concentrations ranging from 0.1 to 10000 microg/l was investigated with cercariae of Diplostomum spathaceum at three temperatures (12, 20, 25 degrees C) and three levels of water hardness (distilled water, soft water, hard water). Under most environmental conditions survival of cerc...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Morley NJ,Crane M,Lewis JW

    更新日期:2001-09-01 00:00:00

  • Desiccation survival of the infective larvae of the insect parasitic nematode, Heterorhabditis zealandica poinar.

    abstract::Desiccation survival of the insect parasitic nematode Heterorhabditis zealandica upon exposure to various relative humidities on membrane filters and agar plates was determined. Survival was poor once water had been lost from the substrate. A screening experiment indicates that survival would not be improved by adjust...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Surrey MR,Wharton DA

    更新日期:1995-06-01 00:00:00

  • Assessment of the exposure to Echinococcus multilocularis associated with carnivore faeces using real-time quantitative PCR and flotation technique assays.

    abstract::The eggs of Echinococcus multilocularis, the infectious stage, are spread into the environment through wild and domestic carnivore faeces. The spatial location of the faeces containing infective E. multilocularis eggs is a key parameter for studying areas of exposure and understanding the transmission processes to the...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Da Silva AM,Courquet S,Raoul F,Rieffel D,Giraudoux P,Millon L,Knapp J

    更新日期:2020-12-01 00:00:00

  • Changed gluten immunity in celiac disease by Necator americanus provides new insights into autoimmunity.

    abstract::We recently completed clinical trials in people with diet-treated celiac disease who were purposefully infected with the ubiquitous human hookworm, Necator americanus. Hookworm infection elicited not only parasite-specific immunity but also modified the host's immune response to gluten. After infection, mucosal IL-1β ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Croese J,Gaze ST,Loukas A

    更新日期:2013-03-01 00:00:00

  • Parasitic diseases of remote Indigenous communities in Australia.

    abstract::Indigenous Australians suffer significant disadvantage in health outcomes and have a life expectancy well below that of non-Indigenous Australians. Mortality rates of Indigenous Australians are higher than that of Indigenous populations in developed countries elsewhere in the world. A number of parasitic diseases whic...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Holt DC,McCarthy JS,Carapetis JR

    更新日期:2010-08-15 00:00:00

  • How adaptive is parasite species diversity?

    abstract::Has species diversity in parasites evolved as a by-product of adaptive diversification driven by competition for limited resources? Or is it a result of gradual genetic drift in isolation? One can move closer to answering these questions by evaluating the ubiquity of host switching, the key stage of adaptive diversifi...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Emelianov I

    更新日期:2007-07-01 00:00:00

  • Studies on the presence and release of proteolytic enzymes (proteinases) in gastro-intestinal nematodes of ruminants.

    abstract::The proteolytic activity of worm homogenates prepared from the third stage larval (L3) and adult stages of the ovine gastro-intestinal nematodes, Nematodirus battus, Ostertagia circumcincta, Trichostrongylus colubriformis, Trichostrongylus vitrinus and Haemonchus contortus, and the rodent intestinal nematode, Nippostr...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Knox DP,Jones DG

    更新日期:1990-04-01 00:00:00

  • Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants.

    abstract::The giant liver fluke, Fascioloides magna, a liver parasite of free-living and domestic ruminants of Europe and North America, was analysed in order to determine the origin of European populations and to reveal the biogeography of this originally North American parasite on the European continent. The variable fragment...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Králová-Hromadová I,Bazsalovicsová E,Stefka J,Spakulová M,Vávrová S,Szemes T,Tkach V,Trudgett A,Pybus M

    更新日期:2011-03-01 00:00:00

  • Structure and diversity of cestode epithelia.

    abstract::Eucestodes have a simple aceolomate body structure and are conservative in terms of the numbers and nature of their cell types. Nevertheless, the cestodes form a diverse group with many strikingly different forms distinguished by the structure of their holdfasts, sexual tissues and embryos. These morphological adaptat...

    journal_title:International journal for parasitology

    pub_type: 杂志文章,评审


    authors: Jones MK

    更新日期:1998-06-01 00:00:00

  • Developmental regulation of protein kinase A expression and activity in Schistosoma mansoni.

    abstract::cAMP-dependent protein kinases (PKAs) are the main transducers of cAMP signalling in eukaryotic cells. Recently we reported the identification and characterisation of a PKA catalytic subunit (SmPKA-C) in Schistosoma mansoni that is required for adult schistosome viability in vitro. To gain further insights into the ro...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Swierczewski BE,Davies SJ

    更新日期:2010-07-01 00:00:00

  • Effect of anti-immunoglobulin antibodies produced in cattle infected with Trypanosoma evansi on antigen detection ELISA.

    abstract::The possibility of interference with the antigen-detection ELISA for trypanosomosis by anti-rodent IgG antibodies produced in cattle infected with Trypanosoma evansi was investigated. Two different ELISA for detection of trypanosome antigen and three different systems for anti-rodent IgG antibody detection were establ...

    journal_title:International journal for parasitology

    pub_type: 杂志文章


    authors: Kashiwazaki Y,Thammasart S

    更新日期:1998-09-01 00:00:00