Abstract:
OBJECTIVE:To map the occurrence of amyloid types in a large clinical cohort using mass spectrometry-based shotgun proteomics, an unbiased method that unambiguously identifies all amyloid types in a single assay. METHODS:A mass spectrometry-based shotgun proteomics assay was implemented in a central reference laboratory. We documented our experience of typing 16,175 amyloidosis specimens over an 11-year period from January 1, 2008, to December 31, 2018. RESULTS:We identified 21 established amyloid types, including AL (n=9542; 59.0%), ATTR (n=4600; 28.4%), ALECT2 (n=511; 3.2%), AA (n=463; 2.9%), AH (n=367; 2.3%), AIns (n=182; 1.2%), KRT5-14 (n=94; <1%), AFib (n=71; <1%), AApoAIV (n=57; <1%), AApoA1 (n=56; <1%), AANF (n=47; <1%), Aβ2M (n=38; <1%), ASem1 (n=34; <1%), AGel (n=29; <1%), TGFB1 (n=29; <1%), ALys (n=15; <1%), AIAPP (n=13; <1%), AApoCII (n=11; <1%), APro (n=8; <1%), AEnf (n=6; <1%), and ACal (n=2; <1%). We developed the first comprehensive organ-by-type map showing the relative frequency of 21 amyloid types in 31 different organs, and the first type-by-organ map showing organ tropism of 18 rare types. Using a modified bioinformatics pipeline, we detected amino acid substitutions in cases of hereditary amyloidosis with 100% specificity. CONCLUSION:Amyloid typing by proteomics, which effectively recognizes all amyloid types in a single assay, optimally supports the diagnosis and treatment of amyloidosis patients in routine clinical practice.
journal_name
Mayo Clin Procjournal_title
Mayo Clinic proceedingsauthors
Dasari S,Theis JD,Vrana JA,Rech KL,Dao LN,Howard MT,Dispenzieri A,Gertz MA,Hasadsri L,Highsmith WE,Kurtin PJ,McPhail EDdoi
10.1016/j.mayocp.2020.06.029subject
Has Abstractpub_date
2020-09-01 00:00:00pages
1852-1864issue
9eissn
0025-6196issn
1942-5546pii
S0025-6196(20)30654-6journal_volume
95pub_type
杂志文章abstract::Primary care physicians are more likely to see patients with depression than with any other disorder except hypertension, and its management poses a challenge to busy primary care practices. The Patient Health Questionnaire-9, a simple self-administered tool of proven validity and reliability, is a commonly used scree...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章,评审
doi:10.4065/82.11.1395
更新日期:2007-11-01 00:00:00
abstract:OBJECTIVE:To assess the results of laparoscopic splenectomy as a treatment for immune thrombocytopenic purpura (ITP). MATERIAL AND METHODS:We conducted a retrospective study of all patients who underwent laparoscopic splenectomy for ITP at our institution between August 1992 and May 1997. RESULTS:Of 27 patients who u...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.4065/74.1.37
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVE:To describe clinical and laboratory findings from the 2012 southeastern Minnesota pertussis outbreak. PATIENTS AND METHODS:Patients were selected for 2 parts of the study. In the first part, nasopharyngeal swabs from a convenience sample of 265 unique patients were used for both the clinician-requested polym...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2014.08.004
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To provide a practical formula for fresh frozen plasma (FFP) dosing for warfarin reversal. PATIENTS AND METHODS:We reviewed data on all adult patients who received a total of 7778 units of FFP for warfarin reversal at Sentara Norfolk General Hospital (Norfolk, VA) between April 1, 2009, and March 31, 2010. P...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2012.12.011
更新日期:2013-03-01 00:00:00
abstract::Two patients had both scleroderma and a columnar epithelium-lined lower esophagus (Barrett esophagus). Features of Barrett's esophagus included high esophageal strictures in both patients and ulcer craters in the columnar area of one. Biopsy confirmed columnar epithelium in the lower esophagus of each patient. In thes...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::Extending a study of the period 1935-1964, we determined the incidence rates of leukemia in Olmsted County during the decade 1965-1974. In this decade, the overall crude annual incidence rate was 9.5 per 100,000. The ratio of males to females was 2.4 to 1; and the incidence rate was significantly higher among males th...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract:OBJECTIVE:To estimate the incidence of selected systemic autoimmune diseases (SAIDs) in approximately 14,000 male rescue/recovery workers enrolled in the Fire Department of the City of New York (FDNY) World Trade Center (WTC) Health Program and to compare FDNY incidence to rates from demographically similar men in the ...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2015.09.019
更新日期:2016-01-01 00:00:00
abstract::In a retrospective analysis, 63 participants in a cardiac rehabilitation-preventive cardiology program were identified as having low blood concentrations (mean, 34 mg/dl) of high-density lipoprotein cholesterol (HDL-C) and a mean total cholesterol level of 223 mg/dl after 3 months of hygienic measures (aerobic exercis...
journal_title:Mayo Clinic proceedings
pub_type: 临床试验,杂志文章
doi:10.1016/s0025-6196(12)60824-6
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND:In essential tremor and Parkinson disease (PD) tremor, administration of onabotulinumtoxinA via a fixed injection approach improves the tremor, but many patients (30%-70%) develop moderate to severe hand weakness, limiting the use of onabotulinumtoxinA in clinical practice. OBJECTIVE:To evaluate the safety ...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.mayocp.2017.06.010
更新日期:2017-09-01 00:00:00
abstract::In a prospective study of 10 patients who underwent liver transplantation and 10 patients who underwent cholecystectomy, we analyzed the postoperative analgesic requirements and the resultant plasma morphine concentrations. Analgesia was more intense, with less medication, and the plasma morphine concentration was sig...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.4065/74.4.330
更新日期:1999-04-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2018.02.022
更新日期:2018-08-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2013.05.011
更新日期:2013-07-01 00:00:00
abstract:OBJECTIVE:To gain information regarding long-term follow-up in patients with synchronous bilateral solid renal neoplasms in whom renal-preserving surgery is imperative. PATIENTS AND METHODS:We examined our surgical experience and the survival outcome, as evaluated by Kaplan-Meier and log-rank analysis, of 94 patients ...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.4065/75.10.1020
更新日期:2000-10-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/s0025-6196(12)62169-7
更新日期:1990-11-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2017.07.004
更新日期:2017-09-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.4065/80.12.1578
更新日期:2005-12-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2017.03.010
更新日期:2017-08-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/j.mayocp.2015.09.018
更新日期:2016-01-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:10.1016/s0025-6196(12)62163-6
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abstract::Hepatitis C virus infection is common, often silent, and almost always chronic and can lead to cirrhosis and hepatocellular cancer. Deaths related to chronic hepatitis C are expected to increase dramatically in the future. Many cases of infection are asymptomatic and are undiagnosed because of a lack of recognition by...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章,评审
doi:10.1016/S0025-6196(11)63702-6
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章,评审
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更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章,随机对照试验
doi:10.4065/83.11.1218
更新日期:2008-11-01 00:00:00
abstract::Carcinoid tumor of the thymus is a rare neoplasm. Formerly confused with thymoma, a tumor of thymic epithelial cells, carcinoid tumor of the thymus has recently been shown to arise from cells of neuroendocrine origin that are normally present in the thymus. About half of the tumors are either functionally active (usua...
journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章,评审
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更新日期:2005-03-01 00:00:00
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更新日期:2019-09-01 00:00:00
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journal_title:Mayo Clinic proceedings
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00