Abstract:
:Antibiotic biosynthetic gene clusters (BGCs) produce bioactive metabolites that impart a fitness advantage to their producer, providing a mechanism for natural selection. This selection drives antibiotic evolution and adapts BGCs for expression in different organisms, potentially providing clues to improve heterologous expression of antibiotics. Here, we use phage-assisted continuous evolution (PACE) to achieve bioactivity-dependent adaptation of the BGC for the antibiotic bicyclomycin (BCM), facilitating improved production in a heterologous host. This proof-of-principle study demonstrates that features of natural bioactivity-dependent evolution can be engineered to access unforeseen routes of improving metabolic pathways and product yields.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Johnston CW,Badran AH,Collins JJdoi
10.1038/s41467-020-18018-2subject
Has Abstractpub_date
2020-08-21 00:00:00pages
4202issue
1issn
2041-1723pii
10.1038/s41467-020-18018-2journal_volume
11pub_type
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