Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi.

Abstract:

:In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function.

journal_name

Biosci Rep

journal_title

Bioscience reports

authors

de Azevedo Dos Santos L,Taveira GB,da Silva MS,da Silva Gebara R,da Silva Pereira L,Perales J,Teixeira-Ferreira A,de Oliveira Mello É,de Oliveira Carvalho A,Rodrigues R,Gomes VM

doi

10.1042/BSR20200950

subject

Has Abstract

pub_date

2020-08-28 00:00:00

issue

8

eissn

0144-8463

issn

1573-4935

pii

226054

journal_volume

40

pub_type

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