Characterization of a GH3 halophilic β-glucosidase from Pseudoalteromonas and its NaCl-induced activity toward isoflavones.

Abstract:

:A novel β-glucosidase gene was isolated from Pseudoalteromonas sp. GXQ-1 and heterologously expressed in Escherichia coli. The activity of the encoded enzyme, PABGL, toward p-nitrophenyl-β-D-glucopyranoside was increased 8.74-fold by the presence of 3 M NaCl relative to the absence of added NaCl. PABGL hydrolyzed a variety of soy isoflavone substrates. For the conversion of daidzin to daidzein, the production rate was 1.44 mM/h. The addition of NaCl enhanced the hydrolytic activity of PABGL toward daidzin and genistein; the maximum activation by NaCl was 3.48- and 6.79-fold, respectively. This is the first report of a halophilic β-glucosidase from Pseudoalteromonas spp., and represents the β-glucosidase with the highest multiple of activation by NaCl. PABGL exhibits strong potential for applications in food processing and industrial production.

journal_name

Int J Biol Macromol

authors

Qu X,Ding B,Li J,Liang M,Du L,Wei Y,Huang R,Pang H

doi

10.1016/j.ijbiomac.2020.07.300

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

1392-1398

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)34059-9

journal_volume

164

pub_type

杂志文章
  • Interactions and compatibility of 11 S globulin from Vicia faba seeds and sodium salt of carboxymethylcellulose in an aqueous medium.

    abstract::This work studies specific interactions and compatibility between a legumin and a linear carboxylated polysaccharide using gel permeation chromatography, sedimentation analysis, SDS gel electrophoresis, viscometry and phase analysis measurements. It uses the system water/11 S globulin/CMC as a model. Carboxymethylcell...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2005.12.011

    authors: Antonov YA,Dmitrochenko AP,Leontiev AL

    更新日期:2006-02-28 00:00:00

  • Polysaccharides from loquat (Eriobotrya japonica) leaves: Impacts of extraction methods on their physicochemical characteristics and biological activities.

    abstract::Impacts of hot water extraction (HWE), pressurized water extraction (PWE), high-speed shearing homogenization extraction, microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), ultrasound assisted enzymatic extraction, and ultrasound-microwave assisted extraction (UMAE) on physicochemical character...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.12.273

    authors: Fu Y,Li F,Ding Y,Li HY,Xiang XR,Ye Q,Zhang J,Zhao L,Qin W,Gan RY,Wu DT

    更新日期:2020-03-01 00:00:00

  • Cellulosic paper with high antioxidative and barrier properties obtained through incorporation of tannin into kraft pulp fibers.

    abstract::In this work, we prepared novel cellulosic paper by incorporating tannin into the kraft pulp for potential application in active food packaging. The kraft pulp fibers were firstly periodate oxidized to obtain the dialdehyde cellulosic fibers, and then reacted with varied dosages of tannin to incorporate them into the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.06.101

    authors: Ji Y,Xu Q,Jin L,Fu Y

    更新日期:2020-11-01 00:00:00

  • Structure-activity relationship of a recombinant hybrid Manganese superoxide dismutase of Staphylococcus saprophyticus/S. equorum.

    abstract::Recombinant hybrid Manganese superoxide dismutase from Staphyloccus saphropyticus/S. equorum (rMnSODSeq) exhibits stability at high temperatures. The enzyme occurs as a dimer that dissociates around 52°C prior to unfolding of the monomer around 64°C, demonstrating contribution of the dimeric form to stability. Here, s...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.01.096

    authors: Retnoningrum DS,Arumsari S,Artarini A,Ismaya WT

    更新日期:2017-05-01 00:00:00

  • A metagenomic alkaline protease from saline habitat: cloning, over-expression and functional attributes.

    abstract::Metagenomics has opened new horizon to unlock the biotechnological potential for novel enzymes. An alkaline protease gene was obtained from the total environmental DNA extracted from a saline habitat. After cloning and sequencing, it was identified that the protease gene related to uncultivable bacteria (HM219181). Th...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.10.032

    authors: Purohit MK,Singh SP

    更新日期:2013-02-01 00:00:00

  • Elastic and biodegradable chitosan/agarose film revealing slightly acidic pH for potential applications in regenerative medicine as artificial skin graft.

    abstract::The aim of this work was to develop new method for the production of chitosan/agarose (CHN/A) film that could serve as a potential artificial skin substitute for regenerative medicine applications. Within this new production method, the concentrations of biomaterial components (chitosan in CH3COOH and agarose in NaOH)...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.099

    authors: Vivcharenko V,Benko A,Palka K,Wojcik M,Przekora A

    更新日期:2020-12-01 00:00:00

  • Fabrication and characterization of PVA/Gum tragacanth/PCL hybrid nanofibrous scaffolds for skin substitutes.

    abstract::In this work three dimensional biodegradable nanofiberous scaffolds containing poly(ε-caprolactone) (PCL), poly(vinyl alcohol) (PVA) and gum tragacanth (GT) were successfully fabricated through two nozzles electrospinning process. For this purpose, PVA/GT blend (Blend: B) solution (60:40wt%) was injected from one syri...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.10.042

    authors: Zarekhalili Z,Bahrami SH,Ranjbar-Mohammadi M,Milan PB

    更新日期:2017-01-01 00:00:00

  • Characterization of three novel β-galactosidases from Akkermansia muciniphila involved in mucin degradation.

    abstract::The gut microbe Akkermansia (A.) muciniphila becomes increasingly important as its prevalence is inversely correlated with different human metabolic disorders and diseases. This organism is a highly potent degrader of intestinal mucins and the hydrolyzed glycan compounds can then serve as carbon sources for the organi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.01.246

    authors: Kosciow K,Deppenmeier U

    更新日期:2020-04-15 00:00:00

  • Characterization of soluble soybean (SSPS) polysaccharide and development of eco-friendly SSPS/TiO2 nanoparticle bionanocomposites.

    abstract::This research aims to characterization of soluble soybean polysaccharide (SSPS) and development of a biodegradable SSPS nanocomposites prepared using various concentrations of TiO2 nanoparticles. 13C NMR suggested that backbone of SSPS is rhamnogalacturonan [1→4)-α-GalAp-(1/2)-α Rhap(1→]. Weight average molecular weig...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.01.182

    authors: Salarbashi D,Tafaghodi M,Bazzaz BSF,Jafari B

    更新日期:2018-06-01 00:00:00

  • Design, synthesis of novel chitosan derivatives bearing quaternary phosphonium salts and evaluation of antifungal activity.

    abstract::Two novel chitosan derivatives modified with quaternary phosphonium salts were successfully synthesized, including tricyclohexylphosphonium acetyl chitosan chloride (TCPACSC) and triphenylphosphonium acetyl chitosan chloride (TPPACSC), and characterized by FTIR, 1H NMR, and 13C NMR spectra. The degree of substitution ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.04.073

    authors: Tan W,Zhang J,Luan F,Wei L,Chen Y,Dong F,Li Q,Guo Z

    更新日期:2017-09-01 00:00:00

  • Anti-tumor and macrophage activation induced by alkali-extracted polysaccharide from Pleurotus ostreatus.

    abstract::Pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in China. Polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. This study was carried out to elucidate the immunomodulating effects and molecular mech...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.05.045

    authors: Kong F,Li FE,He Z,Jiang Y,Hao R,Sun X,Tong H

    更新日期:2014-08-01 00:00:00

  • Anti-inflammatory potential of probiotic Lactobacillus spp. on carrageenan induced paw edema in Wistar rats.

    abstract::The aim of the present study was to evaluate the anti-inflammatory ability of novel indigenous probiotic Lactobacillus fermentum MCC 2759, L. fermentum MCC 2760 and Lactobacillus delbrueckii MCC 2775 in a carrageenan induced acute inflammatory paw edema model. Probiotic cultures were administered to male Wistar rats v...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.08.044

    authors: Archer AC,Muthukumar SP,Halami PM

    更新日期:2015-11-01 00:00:00

  • Isolation and processing of silk proteins for biomedical applications.

    abstract::Silk proteins of silkworms are chiefly composed of core fibroin protein and glycoprotein sericin that glues fibroin. Unique mechanical properties, cyto-compatibility and controllable biodegradability facilitate the use of fibroin in biomedical applications. Sericin serves as additive in cosmetic and food industries, a...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.06.022

    authors: Kundu B,Kurland NE,Yadavalli VK,Kundu SC

    更新日期:2014-09-01 00:00:00

  • Microencapsulation of lectin anti-cancer agent and controlled release by alginate beads, biosafety approach.

    abstract::Hepatocellular carcinoma (HCC) is considered as one of the most aggressive cancer worldwide. In Egypt, the prevalence of HCC is increasing during last years. Recently, drug-loaded microparticles were used to improve the efficiency of various medical treatments. This study is designed to evaluate the anticancer potenti...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.05.031

    authors: El-Aassar MR,Hafez EE,El-Deeb NM,Fouda MM

    更新日期:2014-08-01 00:00:00

  • Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels.

    abstract::Hydrogen peroxide is converted by catalase to molecular oxygen and water to remove oxidative stress. In this study, catalase immobilization was performed using poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (poly(HEMA-GMA)) cryogels with different amounts of GMA. Catalase adsorption capacity of 298.7 ± 9.9 mg...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.121

    authors: Erol K,Cebeci BK,Köse K,Köse DA

    更新日期:2019-02-15 00:00:00

  • Protective effects of sulfated chitooligosaccharides with different degrees of substitution in MIN6 cells.

    abstract::Chitosan, a naturally occurring biopolymer, has received considerable attention for pharmaceutical applications due to its biocompatible, biodegradable and less toxic properties. In this study, sulfated chitooligosaccharides (COS-S) with different degrees of substitution (DS) were prepared to improve both its water-so...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.09.032

    authors: Lu X,Guo H,Sun L,Zhang L,Zhang Y

    更新日期:2013-01-01 00:00:00

  • Novel insights into the treatment of SARS-CoV-2 infection: An overview of current clinical trials.

    abstract::The emergence of the global pandemic caused by the novel SARS-CoV-2 virus has motivated scientists to find a definitive treatment or a vaccine against it in the shortest possible time. Current efforts towards this goal remain fruitless without a full understanding of the behavior of the virus and its adaptor proteins....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2020.09.204

    authors: Oroojalian F,Haghbin A,Baradaran B,Hemmat N,Shahbazi MA,Baghi HB,Mokhtarzadeh A,Hamblin MR

    更新日期:2020-12-15 00:00:00

  • Copolymers of xylan-derived furfuryl alcohol and natural oligomeric tung oil derivatives.

    abstract::The utilization of vegetable oils as biological oligomers for the synthesis of macromolecular materials has considerably evolved in the last decades, opening the way for the preparation of sophisticated materials based on synthetic processes for the design of polymers with very specific applications. Tung oil (TO), ea...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.095

    authors: Ribeiro BO,Valério VS,Gandini A,Lacerda TM

    更新日期:2020-12-01 00:00:00

  • Development of a new paper based nano-biosensor using the co-catalytic effect of tyrosinase from banana peel tissue (Musa Cavendish) and functionalized silica nanoparticles for voltammetric determination of l-tyrosine.

    abstract::In this paper, a new and facile method for the electrochemical determination of l-tyrosine was designed. First, 3-mercaptopropyl trimethoxysilane-functionalized silica nanoparticles were added to a paper disc. Then, the banana peel tissue and the mediator potassium hexacyanoferrate were dropped onto the paper, respect...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.02.060

    authors: Rahimi-Mohseni M,Raoof JB,Ojani R,Aghajanzadeh TA,Bagheri Hashkavayi A

    更新日期:2018-07-01 00:00:00

  • Characterization of the interaction between recombinant porcine aminopeptidase N and spike glycoprotein of porcine epidemic diarrhea virus.

    abstract::Porcine epidemic diarrhea (PED) has caused huge economic losses to the global pork industry. Infection by its causative agent PED virus (PEDV), an Alpha-coronavirus, was previously proven to be mediated by its spike (S) glycoprotein and a cellular receptor porcine aminopeptidase N (pAPN). Interestingly, some recent st...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.05.167

    authors: Sun YG,Li R,Jiang L,Qiao S,Zhi Y,Chen XX,Xie S,Wu J,Li X,Deng R,Zhang G

    更新日期:2018-10-01 00:00:00

  • Improving special hydrolysis characterization into Talaromyces thermophilus F1208 xylanase by engineering of N-terminal extension and site-directed mutagenesis in C-terminal.

    abstract::The purpose of this study was to gain insights into the hydrolysis characteristics of xylanase in producing xylo-oligosaccharides and to improve these by protein engineering. In this study, a hybrid enzyme (T-XynFM) was created from Talaromyces thermophilus F1208 xylanase (T-Xyn) by replacement of the N-terminal exten...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.12.050

    authors: Li Q,Sun B,Xiong K,Teng C,Xu Y,Li L,Li X

    更新日期:2017-03-01 00:00:00

  • Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment.

    abstract::Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at -20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtaine...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.141

    authors: Araújo D,Alves VD,Lima SAC,Reis S,Freitas F,Reis MAM

    更新日期:2020-08-01 00:00:00

  • Pectin-non-starch nanofibers biocomposites as novel gastrointestinal-resistant prebiotics.

    abstract::Incorporation of nanofibers of chitin (NC), lignocellulose (NLC) and bacterial cellulose (BNC) in pectin was studied to improve prebiotic activity and gastrointestinal resistance of the pectin-nanofibers biocomposites for protection of probiotics under simulated gastrointestinal conditions. The biocomposites were prep...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.10.011

    authors: Khorasani AC,Shojaosadati SA

    更新日期:2017-01-01 00:00:00

  • Potential applications of bacterial cellulose and its composites for cancer treatment.

    abstract::Bacterial cellulose (BC) has received immense interest in medical, pharmaceutical, and other related fields owing to its intrinsic physical, mechanical, and biological features. Its structural features offer an ideal environment for developing composites, thereby further extending its areas of applications. BC was ini...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.12.042

    authors: Islam SU,Ul-Islam M,Ahsan H,Ahmed MB,Shehzad A,Fatima A,Sonn JK,Lee YS

    更新日期:2021-01-31 00:00:00

  • Poly-acrylic acid grafted natural rubber for multi-coated slow release compound fertilizer: Preparation, properties and slow-release characteristics.

    abstract::In this study, a novel multi-coated slow release compound fertilizer based on natural rubber (NR) was prepared and characterized. Firstly, NR was grafted with poly-acrylic acid by in-situ radical solution polymerization to synthesize poly-acrylic acid grafted natural rubber (NR-g-PAA), the reaction conditions were opt...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.01.051

    authors: Cui Y,Xiang Y,Xu Y,Wei J,Zhang Z,Li L,Li J

    更新日期:2020-03-01 00:00:00

  • Fish collagen/alginate/chitooligosaccharides integrated scaffold for skin tissue regeneration application.

    abstract::An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.08.038

    authors: Chandika P,Ko SC,Oh GW,Heo SY,Nguyen VT,Jeon YJ,Lee B,Jang CH,Kim G,Park WS,Chang W,Choi IW,Jung WK

    更新日期:2015-11-01 00:00:00

  • Isoenzyme-specific thermostability of human cytosolic creatine kinase.

    abstract::Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2010.03.025

    authors: Gao YS,Zhao TJ,Chen Z,Li C,Wang Y,Yan YB,Zhou HM

    更新日期:2010-07-01 00:00:00

  • Ginsenoside Rb1 ameliorates autophagy via the AMPK/mTOR pathway in renal tubular epithelial cells in vitro and in vivo.

    abstract::Although ginsenoside Rb1 (G-Rb1) has exerted an inhibitory effect on renal fibrosis and progression of chronic kidney disease (CKD), its mechanism remains unknown. This study aims to explore the anti-fibrosis effect of G-Rb1 in unilateral ureter obstruction (UUO) mouse model and underlying mechanisms in HBSS-induced H...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.060

    authors: Liu X,Chen J,Sun N,Li N,Zhang Z,Zheng T,Li Z

    更新日期:2020-11-15 00:00:00

  • Synthetic biology approaches: Towards sustainable exploitation of marine bioactive molecules.

    abstract::The discovery of genes responsible for the production of bioactive metabolites via metabolic pathways combined with the advances in synthetic biology tools, has allowed the establishment of numerous microbial cell factories, for instance the yeast cell factories, for the manufacture of highly useful metabolites from r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2018.01.149

    authors: Seghal Kiran G,Ramasamy P,Sekar S,Ramu M,Hassan S,Ninawe AS,Selvin J

    更新日期:2018-06-01 00:00:00

  • Effective adsorptive removal of reactive dyes by magnetic chitosan nanoparticles: Kinetic, isothermal studies and response surface methodology.

    abstract::In the present study, magnetic chitosan (MC) was synthesized, characterized, and used as an efficient adsorbent for the removal of Reactive Red 141 (RR-141) and Reactive Yellow 14 (RY-14) dyes. Synthesized magnetic chitosan nanoparticles were used for characterization with the help of scanning electron microscope (SEM...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.042

    authors: Jaafari J,Barzanouni H,Mazloomi S,Amir Abadi Farahani N,Sharafi K,Soleimani P,Haghighat GA

    更新日期:2020-12-01 00:00:00