Abstract:
:Osteochondral defects contain damage to both the articular cartilage and underlying subchon- dral bone, which remains a significant challenge in orthopedic surgery. Layered structure of bone, cartilage and the bone-cartilage interface must be taken into account in the case of biofabrication of the osteochondral (OC) interface. In this study, a dual layered OC interface was bioprinted using a newly developed aspiration-assisted bioprinting (AAB) technique, which has been the first time that scaffold-free bioprinting was applied to OC interface engineering. Tissue spheroids, made of human adipose-derived stem cells (ADSCs), were differentiated in three dimensions (3D) into chondrogenic and osteogenic spheroids, which were confirmed by immunostaining and histology qualitatively, and biochemistry assays and gene expression, quantitatively. Remarkably, the OC interface was bioprinted by accurate positioning of a layer of osteogenic spheroids onto a sacrificial alginate support followed by another layer of chondrogenic spheroids overlaid by the same support. Spheroids in individual zones fused and the maintenance of phenotypes in both zones confirmed the successful biofabrication of the histomorphologically-relevant OC interface. The biofabrication of OC tissue model without the use of polymeric scaffolds unveils great potential not only in regenerative medicine but also in drug testing and disease modeling for osteoarthritis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ayan B,Wu Y,Karuppagounder V,Kamal F,Ozbolat ITdoi
10.1038/s41598-020-69960-6subject
Has Abstractpub_date
2020-08-04 00:00:00pages
13148issue
1issn
2045-2322pii
10.1038/s41598-020-69960-6journal_volume
10pub_type
杂志文章abstract::Infrared detection devices are becoming miniature with micro or nano-scale size. The advantages of downsizing come on the expense of insufficient collection of infrared radiation. Therefore, utilizing nano-plasmonic optical antennas becomes mandatory. However, it is desirable to develop antennas with broad bandwidth, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48648-6
更新日期:2019-08-21 00:00:00
abstract::Salinization is having a major impact on wetlands and its biota worldwide. Specifically, many migratory animals that rely on wetlands are increasingly exposed to elevated salinity on their nonbreeding grounds. Experimental evidence suggests that physiological challenges associated with increasing salinity may disrupt ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07258-w
更新日期:2017-07-31 00:00:00
abstract::We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58917-4
更新日期:2020-02-06 00:00:00
abstract::Acyl carrier protein (ACP) domains act as interaction hubs within modular polyketide synthase (PKS) systems, employing specific protein-protein interactions to present acyl substrates to a series of enzyme active sites. Many domains from the multimodular PKS that generates the toxin mycolactone display an unusually hi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38747-9
更新日期:2019-02-20 00:00:00
abstract::Electronic cigarettes (ECs) are nicotine delivery devices that are proposed as tobacco harm reduction products to smokers. Nicotine delivery from ECs is potentially important in their efficacy as smoking substitutes. Herein, nicotine delivery from using a new-generation EC device (variable-wattage, set at 9 W) was eva...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11269
更新日期:2015-06-17 00:00:00
abstract::Whether 2nd-line-chemotherapy (2LCTX) + best-supportive-care (BSC) benefits patients with advanced biliary tract cancer (aBTC) more than BSC alone is unclear. We therefore conducted a propensity-score-based comparative effectiveness analysis of overall survival (OS) outcomes in 80 patients with metastatic, recurrent, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42069-1
更新日期:2019-04-03 00:00:00
abstract::Anserine/carnosine supplementation improves cerebral blood flow and verbal episodic memory in elderly people, as we previously reported. Anserine's buffering activity is superior to that of carnosine at neutral pH. In human sera, carnosine but not anserine is rapidly cleaved by carnosinase, limiting its effectiveness....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12785-7
更新日期:2017-10-03 00:00:00
abstract::Irreversible electroporation (IRE) is an emerging focal therapy which is demonstrating utility in the treatment of unresectable tumors where thermal ablation techniques are contraindicated. IRE uses ultra-short duration, high-intensity monopolar pulsed electric fields to permanently disrupt cell membranes within a wel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14999
更新日期:2015-10-13 00:00:00
abstract::The variation in energy bandgaps of amorphous oxide semiconducting SiZnSnO (a-SZTO) has been investigated by controlling the oxygen partial pressure (Op). The systematic change in Op during deposition has been used to control the electrical characteristics and energy bandgap of a-SZTO. As Op increased, the electrical ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55807-2
更新日期:2019-12-17 00:00:00
abstract::Colorectal cancer (CRC) treatment is associated with a high morbidity which may result in a reduced health-related quality of life (HRQoL). The pre-operative measurement of handgrip strength (HGS) might be a tool to predict the patient's outcome after CRC surgery. The aim of this study was to evaluate the association ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69806-1
更新日期:2020-08-03 00:00:00
abstract::In day-to-day life, humans usually perceive the location of sound sources as outside their heads. This externalized auditory spatial perception can be reproduced through headphones by recreating the sound pressure generated by the source at the listener's eardrums. This requires the acoustical features of the recordin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37342
更新日期:2016-11-17 00:00:00
abstract::Since the hemorrhage in severe dengue seems to be primarily related to the defect of the platelet, the possibility that dengue virus (DENV) is selectively tropic for one of its surface receptors was investigated. Flow cytometric data of DENV-infected megakaryocytic cell line superficially expressing human glycoprotein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20914-z
更新日期:2018-02-09 00:00:00
abstract::Plasmonic photothermal therapy (PPTT) using plasmonic nanoparticles as efficient photoabsorbing agents has been proposed previously. One critical step in PPTT is to effectively deliver gold nanoparticles into the cells. This study demonstrates that the delivery of gold nanorods (AuNRs) can be greatly enhanced by combi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05685
更新日期:2014-07-14 00:00:00
abstract::Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16363-9
更新日期:2017-12-12 00:00:00
abstract::The formation of proper sensory afferent connections during development is essential for brain function. Activity-based competition is believed to drive ocular dominance columns (ODC) in mammals and in experimentally-generated three-eyed frogs. ODC formation is thus a compromise of activity differences between two eye...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08338
更新日期:2015-02-09 00:00:00
abstract::Lanzhou lamb rotavirus vaccine (LLR) is an oral live attenuated vaccine first licensed in China in 2000. To date, > 60 million doses of LLR have been distributed to children. However, very little is known about faecal shedding of LLR in children. Therefore, faecal samples (n = 1,184) were collected from 114 children f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19469-w
更新日期:2018-01-17 00:00:00
abstract::Knowledge of the ecology of N2-fixing (diazotrophic) plankton is mainly limited to oligotrophic (sub)tropical oceans. However, diazotrophs are widely distributed and active throughout the global ocean. Likewise, relatively little is known about the temporal dynamics of diazotrophs in productive areas. Between February...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39586-4
更新日期:2019-03-06 00:00:00
abstract::The ejection of internal organs, i.e., evisceration, is a well-known phenomenon in sea-cucumbers. We report the ability of a member of the Chordate phyla, the tropical ascidian Polycarpa mytiligera, to eviscerate and regenerate its gut within 12 days, and to rebuild its branchial sac within 19 days. Evisceration occur...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09614
更新日期:2015-04-16 00:00:00
abstract::While molecular-targeted drugs have demonstrated strong therapeutic efficacy against diverse diseases such as cancer and infection, the appearance of drug resistance associated with genetic variations in individual patients or pathogens has severely limited their clinical efficacy. Therefore, precision medicine approa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58877-9
更新日期:2020-02-07 00:00:00
abstract::Plant essential oils are considered as important bio-sources for the development of natural and environmentally safe pest control tools due to their multiple modes of action on insects. In this paper we have evaluated the activity of commercially available thyme oil and its constituents thymol, carvacrol, and p-cymene...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79550-1
更新日期:2021-01-13 00:00:00
abstract::Known γ-secretase inhibitors or modulators display an undesirable pharmacokinetic profile and toxicity and have therefore not been successful in clinical trials for Alzheimer's disease (AD). So far, no compounds from natural products have been identified as direct inhibitors of γ-secretase. To search for bioactive mol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16541
更新日期:2015-11-16 00:00:00
abstract::Although silicon (Si) has been widely reported to alleviate plant nutrient deficiency, the underlying mechanism in potassium (K) deficiency is poorly understood. In this study, sorghum seedlings were treated with Si under a K deficiency condition for 15 days. Under control conditions, plant growth was not affected by ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22882
更新日期:2016-03-10 00:00:00
abstract::Food and beverage consumption has a great impact on the environment, although there is a lack of information concerning the whole diet. The environmental impact of 153 Italian adults (51 omnivores, 51 ovo-lacto-vegetarians, 51 vegans) and the inter-individual variability within dietary groups were assessed in a real-l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06466-8
更新日期:2017-07-21 00:00:00
abstract::To develop and externally validate a prostate health index (PHI)-based nomogram for predicting the presence of prostate cancer (PCa) at biopsy in Chinese men with prostate-specific antigen 4-10 ng/mL and normal digital rectal examination (DRE). 347 men were recruited from two hospitals between 2012 and 2014 to develop...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15341
更新日期:2015-10-16 00:00:00
abstract::Congruence theory has many applications in physical, social, biological and technological systems. Congruence arithmetic has been a fundamental tool for data security and computer algebra. However, much less attention was devoted to the topological features of congruence relations among natural numbers. Here, we explo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23714
更新日期:2016-03-31 00:00:00
abstract::Disseminated tumor cells (DTCs) in the bone marrow (BM) and circulating tumor cells (CTCs) in blood of breast cancer patients (pts) are known to correlate with worse outcome. Here we demonstrate a different prognostic value of DTCs and CTCs and explain these findings by early clodronate intake. CTCs (n = 376 pts) were...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26355
更新日期:2016-05-23 00:00:00
abstract::This study analyzed hourly mass concentration observations of PM2.5 (particulate matters with diameter less than 2.5 μm) at 512 stations in China from December 2013 to May 2015. We found that the mean concentrations of PM2.5 during the winter and spring of 2015 Dec. 2014 to Feb. 2015 and Mar. 2015 to May 2015) decreas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36404
更新日期:2016-11-02 00:00:00
abstract::The 3D MHD nonlinear radiative hybrid nanofluid flow across an irregular dimension sheet with slip effect is studied numerically. The hybrid nanofluid consists of copper oxide (CuO) and magnesium oxide (MgO) nanoparticles embedded in methanol or methyl alcohol (MA). The governing PDEs' are altered as ODEs' using simil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66102-w
更新日期:2020-06-08 00:00:00
abstract::Enrofloxacin-loaded docosanoic acid solid lipid nanoparticles (SLNs) with different physicochemical properties were developed to enhance activity against intracellular Salmonella. Their cellular uptake, intracellular elimination and antibacterial activity were studied in RAW 264.7 cells. During the experimental period...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41104
更新日期:2017-01-23 00:00:00
abstract::Resistant starch (RS) has been reported to reduce body fat in obese mice. However, this effect has not been demonstrated in humans. In this study, we tested the effects of RS in 19 volunteers with normal body weights. A randomized, double-blinded and crossover design clinical trial was conducted. The study subjects we...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-018-38216-9
更新日期:2019-03-20 00:00:00