Abstract:
:Dissecting the functions of high molecular weight glutenin subunits (HMW-GSs) is helpful for improving wheat quality via breeding. In this study, we used a wheat mutant AS273 in which HMW-GS 1Dy12 was silenced to investigate the silencing mechanism of 1Dy12 and its effects on gluten accumulation and flour-processing quality. Results suggested that the expression of 1Dy12 in AS273 was decreased by one fifth during grain development; a stop codon produced by a base mutation (C/T) led to truncated translation; the absence of 1Dy12 stimulated the accumulation of low molecular weight glutenin subunits (LMW-GSs), gliadins, and glutenin macropolymers, and was resulted in larger protein bodies; AS273 had an inferior flour-processing performance. Based on the outputs achieved in this study it is concluded that 1Dy12 makes important contributions to bread, sponge cake and biscuit-processing quality.
journal_name
Food Chemjournal_title
Food chemistryauthors
Chen H,Li S,Liu Y,Liu J,Ma X,Du L,Wang K,Ye Xdoi
10.1016/j.foodchem.2020.127663subject
Has Abstractpub_date
2021-01-15 00:00:00pages
127663eissn
0308-8146issn
1873-7072pii
S0308-8146(20)31525-9journal_volume
335pub_type
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