Abstract：
:Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Casanellas I,Lagunas A,Vida Y,Pérez-Inestrosa E,Andrades JA,Becerra J,Samitier Jdoi
10.3390/ijms21155269subject
Has Abstractpub_date
2020-07-24 00:00:00issue
15issn
1422-0067pii
ijms21155269journal_volume
21pub_type
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