Trajectory analysis quantifies transcriptional plasticity during macrophage polarization.

Abstract:

:In recent years, macrophages have been shown to be tremendously plastic in both in vitro and in vivo settings; however, it remains unclear whether macrophages retain any persistent memory of past polarization states which may then impact their future repolarization to new states. Here, we perform deep transcriptomic profiling at high temporal resolution as macrophages are polarized with cytokines that drive them into "M1" and "M2" molecular states. We find through trajectory analysis of their global transcriptomic profiles that macrophages which are first polarized to M1 or M2 and then subsequently repolarized demonstrate little to no memory of their polarization history. We observe complete repolarization both from M1 to M2 and vice versa, and we find that macrophage transcriptional phenotypes are defined by the current cell microenvironment, rather than an amalgamation of past and present states.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Liu SX,Gustafson HH,Jackson DL,Pun SH,Trapnell C

doi

10.1038/s41598-020-68766-w

subject

Has Abstract

pub_date

2020-07-23 00:00:00

pages

12273

issue

1

issn

2045-2322

pii

10.1038/s41598-020-68766-w

journal_volume

10

pub_type

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