Abstract:
:For malignant pleural mesothelioma (MPM) novel therapeutic strategies are urgently needed. In a previous study, we identified 51 putative cancer genes over-expressed in MPM tissues and cell lines. Here, we deepened the study on nine of them (ASS1, EIF4G1, GALNT7, GLUT1, IGF2BP3 (IMP3), ITGA4, RAN, SOD1, and THBS2) to ascertain whether they are truly mesothelial cancer driver genes (CDGs) or genes overexpressed in an adaptive response to the tumoral progression ("passenger genes"). Through a fast siRNA-based screening, we evaluated the consequences of gene depletion on migration, proliferation, colony formation capabilities, and caspase activities of four MPM (Mero-14, Mero-25, IST-Mes2, and NCI-H28) and one SV40-immortalized mesothelial cell line (MeT-5A) as a non-malignant model. The depletion of EIF4G1 and RAN significantly reduced cell proliferation and colony formation and increased caspase activity. In particular, the findings for RAN resemble those observed for other types of cancer. Thus, we evaluated the in vitro effects of importazole (IPZ), a small molecule inhibitor of the interaction between RAN and importin-β. We showed that IPZ could have effects similar to those observed following RAN gene silencing. We also found that primary cell lines from one out of three MPM patients were sensitive to IPZ. As EIF4G1 and RAN deserve further investigation with additional in vitro and in vivo studies, they emerged as promising CDGs, suggesting that their upregulation could play a role in mesothelial tumorigenesis and aggressiveness. Furthermore, present data propose the molecular pathways dependent on RAN as a putative pharmacological target for MPM patients in the view of a future personalized medicine.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Dell'Anno I,Barbarino M,Barone E,Giordano A,Luzzi L,Bottaro M,Migliore L,Agostini S,Melani A,Melaiu O,Catalano C,Cipollini M,Silvestri R,Corrado A,Gemignani F,Landi Sdoi
10.3390/ijms21144856subject
Has Abstractpub_date
2020-07-09 00:00:00issue
14issn
1422-0067pii
ijms21144856journal_volume
21pub_type
杂志文章abstract::Temperature-responsive photonic coatings are appealing for a variety of applications, including smart windows. However, the fabrication of such reflective polymer coatings remains a challenge. In this work, we report the development of a temperature-responsive, infrared-reflective coating consisting of a polymer-stabi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051803
更新日期:2020-03-06 00:00:00
abstract::The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms141019399
更新日期:2013-09-25 00:00:00
abstract::Levodopa (LD) is used to increase dopamine level for treating Parkinson's disease. The major metabolism of LD to produce dopamine is decarboxylation. In order to understand the metabolism of LD; the electronic structure of levodopa was investigated at the Density Functional DFT/B3LYP level of theory using the 6-311+G*...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13044321
更新日期:2012-01-01 00:00:00
abstract::The present study was designed to probe the effects of Huperzine A (HupA) on diabetes-associated cognitive decline (DACD) using a streptozotocin (STZ)-injected rat model. Diabetic rats were treated with HupA (0.05 and 0.1 mg/kg) for seven weeks. Memory functions were evaluated by the water maze test. Nissl staining wa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15057667
更新日期:2014-05-05 00:00:00
abstract:BACKGROUND:Widespread use of prostate specific antigen (PSA) in screening procedures allowed early identification of an increasing number of prostate cancers (PCas), mainly including indolent cancer. Availability of different therapeutic strategies which have a very different impact on the patient's quality of life sug...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21041184
更新日期:2020-02-11 00:00:00
abstract::Hypoxia is a common environmental stress factor and is associated with fibrogenesis. Matrix metalloproteinase-2 (MMP-2), produced by hepatic stellate cells (HSCs), plays an important role in liver fibrogenesis. However, inconsistent results have been reported on the impact of hypoxia on MMP-2 expression and activity i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12042434
更新日期:2011-01-01 00:00:00
abstract::Sulforaphane (SFN) is a phytocompound belonging to the isothiocyanate family. Although it was also found in seeds and mature plants, SFN is mainly present in sprouts of many cruciferous vegetables, including cabbage, broccoli, cauliflower, and Brussels sprouts. SFN is produced by the conversion of glucoraphanin throug...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21228637
更新日期:2020-11-16 00:00:00
abstract::Free fatty acids (FFAs) are cytotoxic to pancreatic islet β-cells and play a crucial role in the diabetes disease process. A recent study revealed a down-regulation of transcription factor 2 (TCF2) levels during FFA-mediated cytotoxicity in pancreatic β-cells. However, its function during this process and the underlyi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150813317
更新日期:2014-07-30 00:00:00
abstract::One of the ultimate goals of wound healing research is to find effective healing techniques that utilize the regeneration of similar tissues. This involves the modification of various wound dressing biomaterials for proper wound management. The biopolymer chitosan (beta-1,4-D-glucosamine) has natural biocompatibility ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms10031300
更新日期:2009-03-01 00:00:00
abstract::The concentration of five soil heavy metals (Pb, Co, Cr, Cu, Hg) was measured in forty sampling sites in central Transylvania, Romania, regions known as centres of pollution due to the chemical and metallurgical activities. The soil samples were collected from locations where the ground is not sliding and the probabil...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9040434
更新日期:2008-04-01 00:00:00
abstract::Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wo...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21217911
更新日期:2020-10-24 00:00:00
abstract::Extracellular vesicles (EVs), including exosomes and microvesicles, are nano-to-micrometer vesicles released from nearly all cellular types. EVs comprise a mixture of bioactive molecules (e.g., mRNAs, miRNAs, lipids, and proteins) that can be transported to the targeted cells/tissues via the blood or lymph circulation...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21145163
更新日期:2020-07-21 00:00:00
abstract::Members of the mitochondrial carrier (MC) protein family transport various molecules across the mitochondrial inner membrane to interlink steps of metabolic pathways and biochemical processes that take place in different compartments; i.e., are localized partly inside and outside the mitochondrial matrix. MC substrate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20184456
更新日期:2019-09-10 00:00:00
abstract::Staphylococcus aureus AgrC is an important component of the agr quorum-sensing system. AgrC is a membrane-embedded histidine kinase that is thought to act as a sensor for the recognition of environmental signals and the transduction of signals into the cytoplasm. However, the difficulty of expressing and purifying fun...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140918470
更新日期:2013-09-06 00:00:00
abstract::Due to their large spectrum of bioactive properties, much attention has recently been drawn to phlorotannins-i.e., phenolic compounds characteristic from brown macroalgae. This study aimed to evaluate the antioxidant and anti-inflammatory properties of F. vesiculosus phlorotannin extracts and purified fractions. Overa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21186897
更新日期:2020-09-20 00:00:00
abstract::Four transglutaminase (TG) isoforms have been detected in epidermal keratinocytes: TG1, TG2, TG3, and TG5. Except for TG1 and TG3, their contribution to keratinocyte development and structure remains undefined. In this paper, we focused on the roles of TG2 and TG3 in imiquimod-induced psoriasis in mouse skin. We evalu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051566
更新日期:2020-02-25 00:00:00
abstract::The antifungal activity of polygodial, a secondary metabolite extracted from Canelo, on mycelial growth of different Botrytis cinerea isolates has been evaluated. The results show that polygodial affects growth of normal and resistant isolates of B. cinerea with EC50 values ranging between 117 and 175 ppm. In addition...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112251
更新日期:2017-10-27 00:00:00
abstract::Although the genetic alteration of CUB and Sushi multiple domains 1 (CSMD1) is known to be associated with poor prognosis in several cancers, there is a lack of clinical relevance in head and neck cancer. The aim of this study was to offer insight into the clinical significance of CSMD1, utilizing a multimodal approac...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19123996
更新日期:2018-12-12 00:00:00
abstract::For possible future use in Photodynamic Therapy (PDT) and/or Photothermal Therapy (PTT) of cancer and screening of cancer cells a new type of ionic liquid photosensitizer -Cholinium-Purpurin-18 (Chol-Pu-18) - was synthesized and small gold (Au) nanoparticles, stabilized by this photosensitizer were prepared without ad...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms9050864
更新日期:2008-05-01 00:00:00
abstract::The yield losses in cereal crops because of abiotic stress and the expected huge losses from climate change indicate our urgent need for useful traits to achieve food security. The stay-green (SG) is a secondary trait that enables crop plants to maintain their green leaves and photosynthesis capacity for a longer time...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20235837
更新日期:2019-11-20 00:00:00
abstract::The often observed scarcity of physical-chemical and well as toxicological data hampers the assessment of potentially hazardous chemicals released to the environment. In such cases Quantitative Structure-Activity Relationships/Quantitative Structure-Property Relationships (QSAR/QSPR) constitute an obvious alternative ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms10041628
更新日期:2009-04-17 00:00:00
abstract::Oxylipins generated by the lipoxygenase (LOX) pathway play an important role in plant defense against biotic and abiotic stress. In chitosan-treated Adelostemma gracillimum seedlings, obvious accumulation of 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, was detected. Using degenerate primers...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13010540
更新日期:2012-01-01 00:00:00
abstract::Intra-articular injection of platelet-rich plasma (PRP) has been established as a suitable treatment for knee osteoarthritis. Here, we present a double-blind randomized controlled clinical trial, conducted in a public Hospital of the Spanish National Health Care System, to evaluate the efficacy of injecting autologous...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3390/ijms17071064
更新日期:2016-07-02 00:00:00
abstract::The complexity of immune system is tempered by precise regulation to maintain stabilization when exposed to various conditions. A subtle change in gene expression may be magnified when drastic changes are brought about in cellular development and function. Posttranslational modifications (PTMs) timely alter the functi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17101547
更新日期:2016-09-22 00:00:00
abstract::In the present study, we investigated the signal transduction of mutants of the eel follicle-stimulating hormone receptor (eelFSHR). Specifically, we examined the constitutively activating mutant D540G in the third intracellular loop, and four inactivating mutants (A193V, N195I, R546C, and A548V). To directly assess f...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197075
更新日期:2020-09-25 00:00:00
abstract::Evaluation of relationships between molecular modeling structural parameters and high-performance liquid chromatography (HPLC) retention data of 11 cardiovascular system drugs by principal component analysis (PCA) in relation to their pharmacological activity was performed. The six retention data parameters were deter...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11072681
更新日期:2010-07-09 00:00:00
abstract::Epithelial cell adhesion molecule (EpCAM) is overexpressed in 55%-75% of ovarian carcinomas (OC). EpCAM might be used as a target for a treatment of disseminated OC. Designed ankyrin repeats protein (DARPin) Ec1 is a small (18 kDa) protein, which binds to EpCAM with subnanomolar affinity. We tested a hypothesis that E...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21093310
更新日期:2020-05-07 00:00:00
abstract::Tear stability decreases with increasing age and the same signs of instability are exacerbated with dry eye. Meibum lipid compositional changes with age provide insights into the biomolecules responsible for tear film instability. Meibum was collected from 69 normal donors ranging in age from 0.6 to 68 years of age. I...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18091862
更新日期:2017-08-28 00:00:00
abstract::Winter's advent invokes physiological adjustments that permit temperate ectotherms to cope with stresses such as food shortage, water deprivation, hypoxia, and hypothermia. We used liquid chromatography (LC) in combination with tandem mass spectrometry (MS/MS) quantitative isobaric (iTRAQ™) peptide mapping to assess v...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12128406
更新日期:2011-01-01 00:00:00
abstract::The human papillomavirus type 16 (HPV16) life cycle can be divided into an early stage in which the HPV16 genomic DNA is replicated, and a late stage in which the HPV16 structural proteins are synthesized and virions are produced. A strong coupling between the viral life cycle and the differentiation state of the infe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18020366
更新日期:2017-02-09 00:00:00