Abstract：
:Weight regulation and the magnitude of weight loss after a Roux-en-Y gastric bypass (RYGB) can be genetically determined. DNA methylation patterns and the expression of some genes can be altered after weight loss interventions, including RYGB. The present study aimed to evaluate how the gene expression and DNA methylation of PIK3R1, an obesity and insulin-related gene, change after RYGB. Blood samples were obtained from 13 women (35.9 ± 9.2 years) with severe obesity before and six months after surgical procedure. Whole blood transcriptome and epigenomic patterns were assessed by microarray-based, genome-wide technologies. A total of 1966 differentially expressed genes were identified in the pre- and postoperative periods of RYGB. From these, we observed that genes involved in obesity and insulin pathways were upregulated after surgery. Then, the PIK3R1 gene was selected for further RT-qPCR analysis and cytosine-guanine nucleotide (CpG) sites methylation evaluation. We observed that the PI3KR1 gene was upregulated, and six DNA methylation CpG sites were differently methylated after bariatric surgery. In conclusion, we found that RYGB upregulates genes involved in obesity and insulin pathways.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Pinhel MAS,Noronha NY,Nicoletti CF,Pereira VA,de Oliveira BA,Cortes-Oliveira C,Salgado W Jr,Barbosa F Jr,Marchini JS,Souza DR,Nonino CBdoi
10.3390/ijms21124476subject
Has Abstractpub_date
2020-06-24 00:00:00issue
12issn
1422-0067pii
ijms21124476journal_volume
21pub_type
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