Abstract：
:Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Safarian N,Whyte-Fagundes P,Zoidl C,Grigull J,Zoidl Gdoi
10.1038/s41598-020-66378-ysubject
Has Abstractpub_date
2020-06-12 00:00:00pages
9538issue
1issn
2045-2322pii
10.1038/s41598-020-66378-yjournal_volume
10pub_type
杂志文章abstract：:Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23165
更新日期：2016-03-17 00:00:00
abstract：:Chiral optical response is an inherent property of molecules and nanostructures, which cannot be superimposed on their mirror images. In specific cases, optical chirality can be observed also for symmetric structures. This so-called extrinsic chirality requires that the mirror symmetry is broken by the geometry of the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41615-1
更新日期：2019-03-25 00:00:00
abstract：:Ionotropic activation of NMDA receptors (NMDARs) requires agonist glutamate and co-agonist glycine. Here we show that glycine enhances the activation of cell survival-promoting kinase Akt in cultured cortical neurons in which both the channel activity of NMDARs and the glycine receptors are pre-inhibited. The effect o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34459
更新日期：2016-10-03 00:00:00
abstract：:Functional neuroimaging, using genetically-encoded Ca2+ sensors in larval zebrafish, offers a powerful combination of high spatiotemporal resolution and higher vertebrate relevance for quantitative neuropharmacological profiling. Here we use zebrafish larvae with pan-neuronal expression of GCaMP6s, combined with light...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06646-6
更新日期：2017-07-26 00:00:00
abstract：:Rotaviruses (RVs) are the most common cause of severe diarrheal disease. To date two rotavirus oral vaccines are licensed: Rotarix and Rotateq. Our aim was to contribute to the post-marketing evaluation of these vaccines safety profile. We collected all RV vaccines-related reports of Adverse Events Following Immunizat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70653-3
更新日期：2020-08-12 00:00:00
abstract：:In this report, spherical silver nanoparticle (AgNP-sp) and rod-shaped silver nanoparticle (AgNR) were prepared by chemical reduction method and their antibacterial activity against various Gram-positive and Gram-negative bacteria had been evaluated for their efficiency. Minimal inhibitory concentration (MIC) tests we...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18590-6
更新日期：2018-01-09 00:00:00
abstract：:Ocean acidification is expected to have detrimental consequences for the most abundant calcifying phytoplankton species Emiliania huxleyi. However, this assumption is mainly based on laboratory manipulations that are unable to reproduce the complexity of natural ecosystems. Here, E. huxleyi coccolith assemblages colle...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59375-8
更新日期：2020-02-13 00:00:00
abstract：:Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light. However, these approaches are often impracticable, for example in clinical therapeutic genome editing in situ or gene...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28178-3
更新日期：2018-07-03 00:00:00
abstract：:The adaptation of herbivorous insects to various host plants facilitates the spread and outbreak of many important invasive pests, however, the molecular mechanisms that underneath this process are poorly understood. In the past three decades, two species of the whitefly Bemisia tabaci complex, Middle East-Asia Minor ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12387-3
更新日期：2017-09-21 00:00:00
abstract：:Understanding the complex dynamics of cardio-respiratory coupling sheds light on the underlying mechanisms governing the communication between these two physiological systems. Previous research has predominantly considered the coupling at respiratory rates slower than the heart rate and shown that respiratory oscillat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38058-5
更新日期：2019-02-07 00:00:00
abstract：:Synovial fibroblasts (SF) were reported to produce B cell activating factor (BAFF) in response to stimulation with interferon-γ (IFN-γ) or tumor necrosis factor (TNF). However, the influence of these pro-inflammatory cytokines on other receptors/ligands of the TNF superfamily or associated cytokine receptors in SF has...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57772-7
更新日期：2020-01-21 00:00:00
abstract：:Local polarization of a magnetic layer, a well-known method for storing information, has found its place in numerous applications such as the popular magnetic drawing board toy or the widespread credit cards and computer hard drives. Here we experimentally show that a similar principle can be applied for imprinting th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27159
更新日期：2016-06-06 00:00:00
abstract：:A key pathophysiological process and therapeutic target in the critical early post-injury period of traumatic brain injury (TBI) is cell mitochondrial dysfunction; characterised by elevation of brain lactate/pyruvate (L/P) ratio in the absence of hypoxia. We previously showed that succinate can improve brain extracell...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29255-3
更新日期：2018-07-24 00:00:00
abstract：:Active immunotherapy targeting β-amyloid (Aβ) is the most promising strategy to prevent or treat Alzheimer's disease (AD). Based on pre-clinical studies and clinical trials, a safe and effective AD vaccine requires a delicate balance between providing therapeutically adequate anti-Aβ antibodies and eliminating or supp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07771
更新日期：2015-01-14 00:00:00
abstract：:The innate and adaptive immune systems fail to control HCV infection in the majority of infected individuals. HCV is an ssRNA virus, which suggests a role for Toll-like receptors (TLRs) 7 and 8 in initiating the anti-viral response. Here we demonstrate that HCV genomic RNA harbours specific sequences that initiate an ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29447
更新日期：2016-07-07 00:00:00
abstract：:We study a three-mode (i.e., a clockwise mode, a counterclockwise mode, and a mechanical mode) coherent coupling regime of the optical whispering-gallery-mode (WGM) microresonator optomechanical system by considering a pair of counterpropagating modes in a general case. The WGM microresonator is coherently driven by a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39781
更新日期：2017-01-03 00:00:00
abstract：:Hologram memory is expected to be the next-generation of optical data storage technology. Bismuth-substituted yttrium iron garnet is typically used for rewritable magnetic hologram media. The diffraction efficiency of magnetic holography depends on the Faraday rotation angle, but the experimental diffraction efficienc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12442-z
更新日期：2017-10-09 00:00:00
abstract：:Pilonidal sinus disease (PSD) is increasing globally. A recent meta-analysis and merged-data analysis showed that recurrence rates in PSD depend essentially on follow-up time and specific surgical procedures. However, the global distribution of surgical approaches and respective recurrence rates have never been studie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51159-z
更新日期：2019-10-22 00:00:00
abstract：:Miscanthus is a rhizomatous C4 grass which is considered as potential high-yielding energy crop with the low-nutrient requirements, high water-use efficiency, and capability of C mitigation. To better understand the genetic basis, an integrative analysis of the transcriptome and proteome was performed to identify impo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14151-z
更新日期：2017-10-23 00:00:00
abstract：:This study aimed to define the width and length of the dental arch in 12-year-old Vietnamese children, and to elucidate differences between genders and among ethnic groups. A cross-sectional study was conducted in 4565 12 years-old children from the 4 major ethnic groups in Vietnam (Kinh, Muong, Thai, and Tay), with a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39710-4
更新日期：2019-02-28 00:00:00
abstract：:Spiny mice of the genus Acomys display several unique physiological traits, including menstruation and scar-free wound healing; characteristics that are exceedingly rare in mammals, and of considerable interest to the scientific community. These unique attributes, and the potential for spiny mice to accurately model h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09334-7
更新日期：2017-08-21 00:00:00
abstract：:This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22598-x
更新日期：2018-03-14 00:00:00
abstract：:Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal forms of cancer. One major reason for this is that PDAC quickly metastasizes to other organs, thereby making its treatment difficult. The molecular machinery driving PDAC metastasis is still poorly understood. In this study, we applied an unbiased appro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58448-y
更新日期：2020-01-30 00:00:00
abstract：:Autism is a common and often severe neurodevelopmental disorder for which diverse pathophysiological processes have been proposed. Recent gene expression data comparing autistic and control brains suggest that the normal differential gene expression between frontal and temporal cortex is attenuated in autistic brains....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01831
更新日期：2013-01-01 00:00:00
abstract：:Research on argillite as a possible host rock for nuclear waste disposal is still an open subject since many issues need to be clarified. In the Underground Research Laboratories constructed for this purpose, a damaged zone around the excavation has been systematically observed and characterized by the appearance of m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08815
更新日期：2015-03-06 00:00:00
abstract：:Rice paddies are a major source of anthropogenic nitrous oxide (N2O) emissions, especially under alternate wetting-drying irrigation and high N input. Increasing photosynthate allocation to the grain in rice (Oryza sativa L.) has been identified as an effective strategy of genetic and agronomic innovation for yield en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29333
更新日期：2016-07-05 00:00:00
abstract：:Micro/nanophotonic structures that are capable of optical wave-front shaping are implemented in optical waveguides and passive optical devices to alter the phase of the light propagating through them. The beam division directions and beam power distribution depend on the design of the micro/nanostructures. The ultimat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44333
更新日期：2017-03-09 00:00:00
abstract：:CRISPR/Cas9-derived RNA-guided nucleases (RGNs) are DNA targeting systems, which are rapidly being harnessed for gene regulation and gene editing purposes in model organisms and cell lines. As bona fide gene delivery vehicles, viral vectors may be particularly fit to broaden the applicability of RGNs to other cell typ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05105
更新日期：2014-05-29 00:00:00
abstract：:We have previously shown that MicroRNA (miR) -144 is a key modulator of the acute cardioprotection associated with remote ischemic preconditioning and post myocardial infarction (MI) remodeling. In this study we examine the biology of the remodeling response after permanent ligation of the left anterior descending cor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35314-6
更新日期：2018-11-15 00:00:00
abstract：:The physical properties of polycrystalline materials depend on their microstructure, which is the nano- to centimeter scale arrangement of phases and defects in their interior. Such microstructure depends on the shape, crystallographic phase and orientation, and interfacing of the grains constituting the material. Thi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09717-w
更新日期：2017-08-25 00:00:00
