Membrane Elastic Properties During Neural Precursor Cell Differentiation.

Abstract:

:Neural precursor cells differentiate into several cell types that display distinct functions. However, little is known about how cell surface mechanics vary during the differentiation process. Here, by precisely measuring membrane tension and bending modulus, we map their variations and correlate them with changes in neural precursor cell morphology along their distinct differentiation fates. Both cells maintained in culture as neural precursors as well as those plated in neurobasal medium reveal a decrease in membrane tension over the first hours of culture followed by stabilization, with no change in bending modulus. During astrocyte differentiation, membrane tension initially decreases and then increases after 72 h, accompanied by consolidation of glial fibrillary acidic protein expression and striking actin reorganization, while bending modulus increases following observed alterations. For oligodendrocytes, the changes in membrane tension are less abrupt over the first hours, but their values subsequently decrease, correlating with a shift from oligodendrocyte marker O4 to myelin basic protein expressions and a remarkable actin reorganization, while bending modulus remains constant. Oligodendrocytes at later differentiation stages show membrane vesicles with similar membrane tension but higher bending modulus as compared to the cell surface. Altogether, our results display an entire spectrum of how membrane elastic properties are varying, thus contributing to a better understanding of neural differentiation from a mechanobiological perspective.

journal_name

Cells

journal_title

Cells

authors

Soares J,Araujo GRS,Santana C,Matias D,Moura-Neto V,Farina M,Frases S,Viana NB,Romão L,Nussenzveig HM,Pontes B

doi

10.3390/cells9061323

subject

Has Abstract

pub_date

2020-05-26 00:00:00

issue

6

issn

2073-4409

pii

cells9061323

journal_volume

9

pub_type

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