Abstract:
:Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems and often revealed complex and unknown microbiomes. In many organisms, the microbiome outnumbers the host cells and greatly affects the host biology and fitness. Ticks are hematophagous ectoparasites with a wide host range. They vector a number of human and animal pathogens and also directly cause major economic losses in livestock. Although several reports on a tick midgut microbiota show a diverse bacterial community, in most cases the size of the bacterial population has not been determined. In this study, the microbiome was quantified in the midgut and ovaries of the ticks Ixodes ricinus and Rhipicephalus microplus before, during, and after blood feeding. Although the size of bacterial community in the midgut fluctuated with blood feeding, it was overall extremely low in comparison to that of other hematophagous arthropods. In addition, the tick ovarian microbiome of both tick species exceeded the midgut 16S rDNA copy numbers by several orders of magnitude. This indicates that the ratio of a tick midgut/ovary microbiome represents an exception to the general biology of other metazoans. In addition to the very low abundance, the tick midgut diversity in I. ricinus was variable and that is in contrast to that found in the tick ovary. The ovary of I. ricinus had a very low bacterial diversity and a very high and stable bacterial abundance with the dominant endosymbiont, Midichloria sp. The elucidation of this aspect of tick biology highlights a unique tissue-specific microbial-invertebrate host interaction.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Guizzo MG,Neupane S,Kucera M,Perner J,Frantová H,da Silva Vaz I,de Oliveira PL,Kopacek P,Zurek Ldoi
10.3389/fcimb.2020.00211subject
Has Abstractpub_date
2020-05-08 00:00:00pages
211issn
2235-2988journal_volume
10pub_type
杂志文章abstract::Platycodin D, extract from the root of Platycodon grandiflorum, is one of the most important monomers of the Qinbaiqingfei pellets (Qinbai) that has already been approved as the first Traditional Chinese Medicine for clinic use as an anti-M. pneumoniae agent. Qinbai constituents Scutellaria baicalensis and Platycodon ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00192
更新日期:2015-01-13 00:00:00
abstract::Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors. Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance. However, the e...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.519479
更新日期:2020-10-21 00:00:00
abstract::Influenza is a major public health problem that causes acute respiratory infection in humans. Identification of host factors influencing in disease outcome is critical for recognition of individuals with increased risk. Investigations on the role of rs34481144A and rs12252C IFITM3 polymorphisms in influenza A(H1N1)pdm...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00352
更新日期:2020-07-10 00:00:00
abstract::While microbiome plays key roles in the etiology of multiple sclerosis (MS), its mechanism remains elusive. Here, we conducted a comprehensive metagenome-wide association study (MWAS) of the relapsing-remitting MS gut microbiome (ncase = 26, ncontrol = 77) in the Japanese population, by using whole-genome shotgun sequ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.585973
更新日期:2020-12-11 00:00:00
abstract::Salmonella is a facultative intracellular Gram-negative bacterium, responsible for a wide range of food- and water-borne diseases ranging from gastroenteritis to typhoid fever depending on hosts and serotypes. Salmonella thus represents a major threat to public health. A key step in Salmonella pathogenesis is the inva...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2017.00500
更新日期:2017-12-08 00:00:00
abstract::Mycobacterium bovis (M. bovis) is the pathogen of animals and humans that can replicate in the phagosomes of myeloid cells. Cytosolic detection of bacterial products plays a crucial role in initiating the innate immune response, including autophagy activation and interferon-β (IFN-β) release. Although IFN-β release an...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00169
更新日期:2017-05-05 00:00:00
abstract::Increased numbers of peripheral blood mononucleocytes (PBMC) and increased IFN-γ secretion following in vitro challenge of blood samples with soluble Leishmania antigen (SLA), have been proposed as biomarkers of specific cell-mediated immunity, indicating that treatment of visceral leishmaniasis (VL) has been successf...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00381
更新日期:2018-11-13 00:00:00
abstract::Pneumonia and meningitis continue to present an enormous public health burden and pose a major threat to young children. Among the causative organisms of pneumonia and meningitis, bacteria are the most common causes of serious disease and deaths. It is challenging to accurately and rapidly identify these agents. To so...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00107
更新日期:2018-04-05 00:00:00
abstract::Protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. In this review, we will focus on the recently discovered type IX secre...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2017.00215
更新日期:2017-05-26 00:00:00
abstract::Periodontal microorganisms not only colonize subgingival pockets, but also are detected on various mucous membranes in patients with periodontitis. The object of this pilot study was, using the next-generation sequencing of 16S RNA gene, to characterize the microbiota in two oral habitats (buccal mucosas and subgingiv...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00053
更新日期:2019-03-11 00:00:00
abstract::In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of Nocardia spp. but also greatly reduce the turnaround...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00013
更新日期:2020-02-18 00:00:00
abstract::To define the bottlenecks that restrict antigen expression after oral administration of viral-vectored vaccines, we tracked vectors derived from the human adenovirus type 5 at whole body, tissue, and cellular scales throughout the digestive tract in a murine model of oral delivery. After intragastric administration of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00006
更新日期:2018-01-25 00:00:00
abstract::Gut microbiota serves as a critical indicator for gut health during treatment of pathogenic bacterial infection. Both Pulsatilla Decoction (abbreviated to PD, a traditional Chinese medicine compound) and Levofloxacin Hydrochloride (LVX) were known to have therapeutic effects to intestinal infectious disease. However, ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00319
更新日期:2020-06-30 00:00:00
abstract::Overwhelming cardiac inflammation has been reported to be the pathogenic mechanism of Coxsackievirus B3 (CVB3)-induced viral myocarditis (VMC), while the detailed molecular mechanisms remain unknown. Membrane-bound dipeptidases (MBD, also known as Dpep) have been shown to be involved in inflammatory diseases. However,...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00057
更新日期:2019-03-07 00:00:00
abstract::Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii. The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial me...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00472
更新日期:2020-02-07 00:00:00
abstract::The worldwide increase in antibiotic-resistant pathogens means that identification of alternative antibacterial drug targets and the subsequent development of new treatment strategies are urgently required. One such new target is the quorum sensing (QS) system. Coral microbial consortia harbor an enormous diversity of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00144
更新日期:2018-05-08 00:00:00
abstract::The spirochete endoflagellum is a unique motility apparatus among bacteria. Despite its critical importance for pathogenesis, the full composition of the flagellum remains to be determined. We have recently reported that FcpA is a novel flagellar protein and a major component of the sheath of the filament of the spiro...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00130
更新日期:2018-05-08 00:00:00
abstract::Francisella tularensis can bypass and suppress host immune responses, even to the point of manipulating immune cell phenotypes and intercellular inflammatory networks. Strengthening these responses such that immune cells more readily identify and destroy the bacteria is likely to become a viable (and perhaps necessary...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2014.00018
更新日期:2014-02-21 00:00:00
abstract::Brucella is a facultative intracellular pathogen that causes zoonotic infection known as brucellosis which results in abortion and infertility in natural host. Humans, especially in low income countries, can acquire infection by direct contact with infected animal or by consumption of animal products and show high mor...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2016.00030
更新日期:2016-03-15 00:00:00
abstract::Toxoplasma gondii is an important human and animal pathogen that causes life-threatening toxoplasmosis. The host immune system produces interferon-γ (IFN-γ) to inhibit T. gondii proliferation. IFN-γ-inducible indole-2,3-dioxygenase 1 (IDO1), which mediates tryptophan degradation, has a major role in anti-T. gondii imm...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00140
更新日期:2019-05-01 00:00:00
abstract::The Mycobacterium fortuitum complex comprises several closely related species, causing pulmonary and extra-pulmonary infections. However, there is very limited knowledge about the disease pathogenesis involved in M. fortuitum infections, particularly due to the lack of suitable animal models. Using the zebrafish model...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00357
更新日期:2020-07-17 00:00:00
abstract::Escherichia coli O157:H7 and other pathogenic E. coli strains are enteric pathogens associated with food safety threats and which remain a significant cause of morbidity and mortality worldwide. In the current study, we investigated whether enterohemorrhagic E. coli (EHEC), Shiga toxin-producing E. coli (STEC), and en...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2012.00008
更新日期:2012-02-14 00:00:00
abstract::Objective:Streptococcus pneumoniae colonizes the nasopharynx of children, and from nasopharynx it could migrate to the middle ear and causes acute otitis media (AOM). During colonization and AOM, the pneumococcus forms biofilms. In vitro biofilm formation requires a functional LuxS/AI-2 quorum-sensing system. We inves...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00138
更新日期:2018-05-04 00:00:00
abstract::Visceral leishmaniasis is a neglected tropical disease caused by Leishmania (L.) donovani parasite in the Indian subcontinent. Macrophages (mϕ) are the harboring cells for parasite and their interactions dictate the pathogenesis of this disease. Polyunsaturated fatty acids are an integral part of the mϕ cell membrane ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00406
更新日期:2020-08-07 00:00:00
abstract::Francisella tularensis is the causative agent of tularemia and a Tier I bioterrorism agent. In the 1900s, several vaccines were developed against tularemia including the killed "Foshay" vaccine, subunit vaccines comprising F. tularensis protein(s) or lipoproteins(s) in an adjuvant formulation, and the F. tularensis Li...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2018.00154
更新日期:2018-05-15 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 EspF is an important multifunctional protein that destroys the tight junctions of intestinal epithelial cells and promotes host cell apoptosis. However, its molecular mechanism remains elusive. We knocked out the espF sequence (747 bp, ΔespF), N-terminal sequence (219 ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00410
更新日期:2017-09-21 00:00:00
abstract::Burkholderia cenocepacia is an opportunistic respiratory pathogen of individuals with cystic fibrosis (CF). Some strains of B. cenocepacia are highly transmissible and resistant to almost all antibiotics. Approximately one-third of B. cenocepacia infected CF patients go on to develop fatal "cepacia syndrome." During t...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2011.00025
更新日期:2012-01-11 00:00:00
abstract::Cold shock-domain family proteins (Csps) are highly conserved nucleic acid binding proteins regulating the expression of various genes including those involved in stress resistance and virulence in bacteria. We show here that Csps are involved in virulence, cell aggregation and flagella-based extracellular motility of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00453
更新日期:2017-10-26 00:00:00
abstract::Osteomyelitis is commonly caused by Staphylococcus aureus. Both erythromycin and curcumin can suppress S. aureus growth, but their roles in osteomyelitis are barely studied. We aim to explore the activities of erythromycin and curcumin against chronical osteomyelitis induced by methicillin-resistant S. aureus (MRSA). ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00379
更新日期:2017-08-24 00:00:00
abstract::Virulent C. difficile toxins TcdA and TcdB invade host intestinal epithelia by endocytosis and use the acidic environment of intracellular vesicles for further processing and activation. We investigated the role of ClC-5, a chloride/proton exchanger expressed in the endosomes of gastrointestinal epithelial cells, in t...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00067
更新日期:2017-03-13 00:00:00