Selection of Endogenous Control Reference Genes for Studies on Type 1 or Type 2 Endometrial Cancer.

Abstract:

:A panel of 32 candidate reference genes was used to identify the most stable genes for gene normalisation in quantitative RT-PCR studies using endometrial biopsies obtained from women with endometrial cancer (type 1 or type 2) and without cancer (controls). RNA from the biopsies was isolated, examined for purity and quality, and then reverse transcribed into cDNA before being subjected to real-time qRT-PCR analysis in triplicate within the TaqMan gene Expression Assay kit. The most 'stable' endogenous control genes were then identified using the geNorm qbase + 2 and NormFinder software packages. PSMC4, PUM1 and IPO8 were identified as the best reference genes combination for type 1 endometrial cancer (grades 1, 2 and 3), whereas for type 2 endometrial cancer (serous and carcinosarcoma), UBC, MRPL19, PGK1 and PPIA were the best reference genes combination. We conclude that the use of these normaliser combinations should provide accurate interpretation of gene expression at the transcript level in endometrial cancer studies especially for types 1 and 2 cancers.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ayakannu T,Taylor AH,Konje JC

doi

10.1038/s41598-020-64663-4

subject

Has Abstract

pub_date

2020-05-21 00:00:00

pages

8468

issue

1

issn

2045-2322

pii

10.1038/s41598-020-64663-4

journal_volume

10

pub_type

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