Development of novel detection system for sweet potato leaf curl virus using recombinant scFv.

Abstract:

:Sweet potato leaf curl virus (SPLCV) causes yield losses in sweet potato cultivation. Diagnostic techniques such as serological detection have been developed because these plant viruses are difficult to treat. Serological assays have been used extensively with recombinant antibodies such as whole immunoglobulin or single-chain variable fragments (scFv). An scFv consists of variable heavy (VH) and variable light (VL) chains joined with a short, flexible peptide linker. An scFv can serve as a diagnostic application using various combinations of variable chains. Two SPLCV-specific scFv clones, F7 and G7, were screened by bio-panning process with a yeast cell which expressed coat protein (CP) of SPLCV. The scFv genes were subcloned and expressed in Escherichia coli. The binding affinity and characteristics of the expressed proteins were confirmed by enzyme-linked immunosorbent assay using SPLCV-infected plant leaves. Virus-specific scFv selection by a combination of yeast-surface display and scFv-phage display can be applied to detection of any virus.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Cho SH,Kil EJ,Cho S,Byun HS,Kang EH,Choi HS,Lee MG,Lee JS,Lee YG,Lee S

doi

10.1038/s41598-020-64996-0

subject

Has Abstract

pub_date

2020-05-15 00:00:00

pages

8039

issue

1

issn

2045-2322

pii

10.1038/s41598-020-64996-0

journal_volume

10

pub_type

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