Abstract:
:Sweet potato leaf curl virus (SPLCV) causes yield losses in sweet potato cultivation. Diagnostic techniques such as serological detection have been developed because these plant viruses are difficult to treat. Serological assays have been used extensively with recombinant antibodies such as whole immunoglobulin or single-chain variable fragments (scFv). An scFv consists of variable heavy (VH) and variable light (VL) chains joined with a short, flexible peptide linker. An scFv can serve as a diagnostic application using various combinations of variable chains. Two SPLCV-specific scFv clones, F7 and G7, were screened by bio-panning process with a yeast cell which expressed coat protein (CP) of SPLCV. The scFv genes were subcloned and expressed in Escherichia coli. The binding affinity and characteristics of the expressed proteins were confirmed by enzyme-linked immunosorbent assay using SPLCV-infected plant leaves. Virus-specific scFv selection by a combination of yeast-surface display and scFv-phage display can be applied to detection of any virus.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Cho SH,Kil EJ,Cho S,Byun HS,Kang EH,Choi HS,Lee MG,Lee JS,Lee YG,Lee Sdoi
10.1038/s41598-020-64996-0subject
Has Abstractpub_date
2020-05-15 00:00:00pages
8039issue
1issn
2045-2322pii
10.1038/s41598-020-64996-0journal_volume
10pub_type
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