Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins.

Abstract:

:The current outbreak of coronavirus disease-2019 (COVID-19) poses unprecedented challenges to global health1. The new coronavirus responsible for this outbreak-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-shares high sequence identity to SARS-CoV and a bat coronavirus, RaTG132. Although bats may be the reservoir host for a variety of coronaviruses3,4, it remains unknown whether SARS-CoV-2 has additional host species. Here we show that a coronavirus, which we name pangolin-CoV, isolated from a Malayan pangolin has 100%, 98.6%, 97.8% and 90.7% amino acid identity with SARS-CoV-2 in the E, M, N and S proteins, respectively. In particular, the receptor-binding domain of the S protein of pangolin-CoV is almost identical to that of SARS-CoV-2, with one difference in a noncritical amino acid. Our comparative genomic analysis suggests that SARS-CoV-2 may have originated in the recombination of a virus similar to pangolin-CoV with one similar to RaTG13. Pangolin-CoV was detected in 17 out of the 25 Malayan pangolins that we analysed. Infected pangolins showed clinical signs and histological changes, and circulating antibodies against pangolin-CoV reacted with the S protein of SARS-CoV-2. The isolation of a coronavirus from pangolins that is closely related to SARS-CoV-2 suggests that these animals have the potential to act as an intermediate host of SARS-CoV-2. This newly identified coronavirus from pangolins-the most-trafficked mammal in the illegal wildlife trade-could represent a future threat to public health if wildlife trade is not effectively controlled.

journal_name

Nature

journal_title

Nature

authors

Xiao K,Zhai J,Feng Y,Zhou N,Zhang X,Zou JJ,Li N,Guo Y,Li X,Shen X,Zhang Z,Shu F,Huang W,Li Y,Zhang Z,Chen RA,Wu YJ,Peng SM,Huang M,Xie WJ,Cai QH,Hou FH,Chen W,Xiao L,Shen Y

doi

10.1038/s41586-020-2313-x

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

286-289

issue

7815

eissn

0028-0836

issn

1476-4687

pii

10.1038/s41586-020-2313-x

journal_volume

583

pub_type

杂志文章

相关文献

NATURE文献大全
  • Maternal control of resting-egg production in Daphnia.

    abstract::Many planktonic organisms produce 'resting' stages when the environmental conditions deteriorate. Like seeds, resting stages can survive unfavourable conditions. The crustacean Daphnia normally reproduces by means of parthenogenetically produced normal, not resting, eggs-but occasionally switches to bisexual reproduct...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/414899a

    authors: Alekseev V,Lampert W

    更新日期:2001-12-20 00:00:00

  • Chondrule formation in particle-rich nebular regions at least hundreds of kilometres across.

    abstract::Chondrules are millimetre-sized spherules (mostly silicate) that dominate the texture of primitive meteorites. Their formation mechanism is debated, but their sheer abundance suggests that the mechanism was both energetic and ubiquitous in the early inner Solar System. The processes suggested--such as shock waves, sol...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature04834

    authors: Cuzzi JN,Alexander CM

    更新日期:2006-05-25 00:00:00

  • The association of GRB 060218 with a supernova and the evolution of the shock wave.

    abstract::Although the link between long gamma-ray bursts (GRBs) and supernovae has been established, hitherto there have been no observations of the beginning of a supernova explosion and its intimate link to a GRB. In particular, we do not know how the jet that defines a gamma-ray burst emerges from the star's surface, nor ho...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature04892

    authors: Campana S,Mangano V,Blustin AJ,Brown P,Burrows DN,Chincarini G,Cummings JR,Cusumano G,Della Valle M,Malesani D,Mészáros P,Nousek JA,Page M,Sakamoto T,Waxman E,Zhang B,Dai ZG,Gehrels N,Immler S,Marshall FE,Mason KO

    更新日期:2006-08-31 00:00:00

  • Molecular envoys pave the way for pancreatic cancer to invade the liver.

    abstract:: ...

    journal_title:Nature

    pub_type: 评论,新闻

    doi:10.1038/d41586-019-00710-z

    authors: Maitra A

    更新日期:2019-03-01 00:00:00

  • Modern freshwater microbialite analogues for ancient dendritic reef structures.

    abstract::Microbialites are organosedimentary structures that can be constructed by a variety of metabolically distinct taxa. Consequently, microbialite structures abound in the fossil record, although the exact nature of the biogeochemical processes that produced them is often unknown. One such class of ancient calcareous stru...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35036579

    authors: Laval B,Cady SL,Pollack JC,McKay CP,Bird JS,Grotzinger JP,Ford DC,Bohm HR

    更新日期:2000-10-05 00:00:00

  • Control of somatic tissue differentiation by the long non-coding RNA TINCR.

    abstract::Several of the thousands of human long non-coding RNAs (lncRNAs) have been functionally characterized; however, potential roles for lncRNAs in somatic tissue differentiation remain poorly understood. Here we show that a 3.7-kilobase lncRNA, terminal differentiation-induced ncRNA (TINCR), controls human epidermal diffe...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature11661

    authors: Kretz M,Siprashvili Z,Chu C,Webster DE,Zehnder A,Qu K,Lee CS,Flockhart RJ,Groff AF,Chow J,Johnston D,Kim GE,Spitale RC,Flynn RA,Zheng GX,Aiyer S,Raj A,Rinn JL,Chang HY,Khavari PA

    更新日期:2013-01-10 00:00:00

  • Molecular manipulation using a tunnelling microscope.

    abstract::In a very short time the scanning tunnelling microscope has become an important tool in surface science, and physics in general. Its primary use has been to obtain atomic-resolution images of surfaces, but recently, efforts have been made to use it to manipulate materials as well as image them. One may now reasonably ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/331324a0

    authors: Foster JS,Frommer JE,Arnett PC

    更新日期:1988-01-28 00:00:00

  • Speech sounds learned by sleeping newborns.

    abstract::It is not yet clear whether humans are able to learn while they are sleeping. Here we show that full-term human newborns can be taught to discriminate between similar vowel sounds when they are fast asleep. It is possible that such sleep training soon after birth could find application in clinical or educational situa...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/415599b

    authors: Cheour M,Martynova O,Näätänen R,Erkkola R,Sillanpää M,Kero P,Raz A,Kaipio ML,Hiltunen J,Aaltonen O,Savela J,Hämäläinen H

    更新日期:2002-02-07 00:00:00

  • DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected].

    abstract::Non-coding apurinic/apyrimidinic (AP) sites in DNA are continually created in cells both spontaneously and by damage-specific DNA glycosylases. The biologically critical human base excision repair enzyme APE1 cleaves the DNA sugar-phosphate backbone at a position 5' of AP sites to prime DNA repair synthesis. Here we r...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35000249

    authors: Mol CD,Izumi T,Mitra S,Tainer JA

    更新日期:2000-01-27 00:00:00

  • Evolution of RNA editing in kinetoplastid protozoa.

    abstract::The editing of RNA in trypanosomatid mitochondria involves the insertion and occasional deletion of uridine residues within coding regions of maxicircle messenger RNA transcripts. The extent to which the transcripts of homologous genes undergo editing differs in different species. In some, entire genes are edited (pan...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/368345a0

    authors: Maslov DA,Avila HA,Lake JA,Simpson L

    更新日期:1994-03-24 00:00:00

  • Inhibition of bacterial ice nucleators by fish antifreeze glycoproteins.

    abstract::Certain bacteria promote the formation of ice in super-cooled water by means of ice nucleators which contain a unique protein associated with the cell membrane. Ice nucleators in general are believed to act by mimicking the structure of an ice crystal surface, thus imposing an ice-like arrangement on the water molecul...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/333782a0

    authors: Parody-Morreale A,Murphy KP,Di Cera E,Fall R,DeVries AL,Gill SJ

    更新日期:1988-06-23 00:00:00

  • Greenland melt drives continuous export of methane from the ice-sheet bed.

    abstract::Ice sheets are currently ignored in global methane budgets1,2. Although ice sheets have been proposed to contain large reserves of methane that may contribute to a rise in atmospheric methane concentration if released during periods of rapid ice retreat3,4, no data exist on the current methane footprint of ice sheets....

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-018-0800-0

    authors: Lamarche-Gagnon G,Wadham JL,Sherwood Lollar B,Arndt S,Fietzek P,Beaton AD,Tedstone AJ,Telling J,Bagshaw EA,Hawkings JR,Kohler TJ,Zarsky JD,Mowlem MC,Anesio AM,Stibal M

    更新日期:2019-01-01 00:00:00

  • Kondo resonance in a single-molecule transistor.

    abstract::When an individual molecule, nanocrystal, nanotube or lithographically defined quantum dot is attached to metallic electrodes via tunnel barriers, electron transport is dominated by single-electron charging and energy-level quantization. As the coupling to the electrodes increases, higher-order tunnelling and correlat...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature00790

    authors: Liang W,Shores MP,Bockrath M,Long JR,Park H

    更新日期:2002-06-13 00:00:00

  • Selective amplification by auto- and cross-catalysis in a replicating peptide system.

    abstract::Self-replication has been demonstrated in synthetic chemical systems based on oligonucleotides, peptides and complementary molecules without natural analogues. However, within a living cell virtually no molecule catalyses its own formation, and the search for chemical systems in which both auto- and cross-catalysis ca...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/24814

    authors: Yao S,Ghosh I,Zutshi R,Chmielewski J

    更新日期:1998-12-03 00:00:00

  • Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal.

    abstract::Monoclonal antibodies were raised against antibodies to distinct carboxy-terminal KDEL sequences of two soluble, resident endoplasmic reticulum proteins. These anti-idiotype reagents recognize an intrinsic membrane protein with characteristics expected of a receptor responsible for the recognition and return of reside...

    journal_title:Nature

    pub_type: 杂志文章,收录出版

    doi:10.1038/345495a0

    authors: Vaux D,Tooze J,Fuller S

    更新日期:1990-06-07 00:00:00

  • Structure of the cro repressor from bacteriophage lambda and its interaction with DNA.

    abstract::The three-dimensional structure of the 66-amino acid cro repressor protein of bacteriophage lambda suggests how it binds to its operator DNA. We propose that a dimer of cro protein is bound to the B-form of DNA with the 2-fold axis of the dimer coincident with the 2-fold axis of DNA. A pair of 2-fold-related alpha-hel...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/290754a0

    authors: Anderson WF,Ohlendorf DH,Takeda Y,Matthews BW

    更新日期:1981-04-30 00:00:00

  • Species-area relationships always overestimate extinction rates from habitat loss.

    abstract::Extinction from habitat loss is the signature conservation problem of the twenty-first century. Despite its importance, estimating extinction rates is still highly uncertain because no proven direct methods or reliable data exist for verifying extinctions. The most widely used indirect method is to estimate extinction...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature09985

    authors: He F,Hubbell SP

    更新日期:2011-05-19 00:00:00

  • HER2 expression identifies dynamic functional states within circulating breast cancer cells.

    abstract::Circulating tumour cells in women with advanced oestrogen-receptor (ER)-positive/human epidermal growth factor receptor 2 (HER2)-negative breast cancer acquire a HER2-positive subpopulation after multiple courses of therapy. In contrast to HER2-amplified primary breast cancer, which is highly sensitive to HER2-targete...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature19328

    authors: Jordan NV,Bardia A,Wittner BS,Benes C,Ligorio M,Zheng Y,Yu M,Sundaresan TK,Licausi JA,Desai R,O'Keefe RM,Ebright RY,Boukhali M,Sil S,Onozato ML,Iafrate AJ,Kapur R,Sgroi D,Ting DT,Toner M,Ramaswamy S,Haas W,Mah

    更新日期:2016-09-01 00:00:00

  • An ancestral mitochondrial DNA resembling a eubacterial genome in miniature.

    abstract::Mitochondria, organelles specialized in energy conservation reactions in eukaryotic cells, have evolved from eubacteria-like endosymbionts whose closest known relatives are the rickettsial group of alpha-proteobacteria. Because characterized mitochondrial genomes vary markedly in structure, it has been impossible to i...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/387493a0

    authors: Lang BF,Burger G,O'Kelly CJ,Cedergren R,Golding GB,Lemieux C,Sankoff D,Turmel M,Gray MW

    更新日期:1997-05-29 00:00:00

  • Chemical and behavioural characterization of the rabbit mammary pheromone.

    abstract::Mammals owe part of their evolutionary success to the harmonious exchanges of information, energy and immunity between females and their offspring. This functional reciprocity is vital for the survival and normal development of infants, and for the inclusive fitness of parents. It is best seen in the intense exchanges...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature01739

    authors: Schaal B,Coureaud G,Langlois D,Giniès C,Sémon E,Perrier G

    更新日期:2003-07-03 00:00:00

  • A trans-acting locus regulates an anti-viral expression network and type 1 diabetes risk.

    abstract::Combined analyses of gene networks and DNA sequence variation can provide new insights into the aetiology of common diseases that may not be apparent from genome-wide association studies alone. Recent advances in rat genomics are facilitating systems-genetics approaches. Here we report the use of integrated genome-wid...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature09386

    authors: Heinig M,Petretto E,Wallace C,Bottolo L,Rotival M,Lu H,Li Y,Sarwar R,Langley SR,Bauerfeind A,Hummel O,Lee YA,Paskas S,Rintisch C,Saar K,Cooper J,Buchan R,Gray EE,Cyster JG,Cardiogenics Consortium.,Erdmann J,Heng

    更新日期:2010-09-23 00:00:00

  • Elevation alters ecosystem properties across temperate treelines globally.

    abstract::Temperature is a primary driver of the distribution of biodiversity as well as of ecosystem boundaries. Declining temperature with increasing elevation in montane systems has long been recognized as a major factor shaping plant community biodiversity, metabolic processes, and ecosystem dynamics. Elevational gradients,...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature21027

    authors: Mayor JR,Sanders NJ,Classen AT,Bardgett RD,Clément JC,Fajardo A,Lavorel S,Sundqvist MK,Bahn M,Chisholm C,Cieraad E,Gedalof Z,Grigulis K,Kudo G,Oberski DL,Wardle DA

    更新日期:2017-02-02 00:00:00

  • Dissociation between hand motion and population vectors from neural activity in motor cortex.

    abstract::The population vector hypothesis was introduced almost twenty years ago to illustrate that a population vector constructed from neural activity in primary motor cortex (MI) of non-human primates could predict the direction of hand movement during reaching. Alternative explanations for this population signal have been ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35093102

    authors: Scott SH,Gribble PL,Graham KM,Cabel DW

    更新日期:2001-09-13 00:00:00

  • Molecular-scale evidence of aerosol particle formation via sequential addition of HIO3.

    abstract::Homogeneous nucleation and subsequent cluster growth leads to the formation of new aerosol particles in the atmosphere. The nucleation of sulfuric acid and organic vapours is thought to be responsible for the formation of new particles over continents, whereas iodine oxide vapours have been implicated in particle form...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature19314

    authors: Sipilä M,Sarnela N,Jokinen T,Henschel H,Junninen H,Kontkanen J,Richters S,Kangasluoma J,Franchin A,Peräkylä O,Rissanen MP,Ehn M,Vehkamäki H,Kurten T,Berndt T,Petäjä T,Worsnop D,Ceburnis D,Kerminen VM,Kulmala M,O'D

    更新日期:2016-09-22 00:00:00

  • Chemical interaction of Fe and Al(2)O3 as a source of heterogeneity at the Earth's core-mantle boundary.

    abstract::Seismological studies have revealed that a complex texture or heterogeneity exists in the Earth's inner core and at the boundary between core and mantle. These studies highlight the importance of understanding the properties of iron when modelling the composition and dynamics of the core and the interaction of the cor...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35087559

    authors: Dubrovinsky L,Annersten H,Dubrovinskaia N,Westman F,Harryson H,Fabrichnaya O,Carlson S

    更新日期:2001-08-02 00:00:00

  • Drosophila CBP represses the transcription factor TCF to antagonize Wingless signalling.

    abstract::T-cell factor (TCF), a high-mobility-group domain protein, is the transcription factor activated by Wnt/Wingless signalling. When signalling occurs, TCF binds to its coactivator, beta-catenin/Armadillo, and stimulates the transcription of the target genes of Wnt/Wingless by binding to TCF-responsive enhancers. Inappro...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/26785

    authors: Waltzer L,Bienz M

    更新日期:1998-10-01 00:00:00

  • Dichloroacetate prevents restenosis in preclinical animal models of vessel injury.

    abstract::Despite the introduction of antiproliferative drug-eluting stents, coronary heart disease remains the leading cause of death in the United States. In-stent restenosis and bypass graft failure are characterized by excessive smooth muscle cell (SMC) proliferation and concomitant myointima formation with luminal oblitera...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature13232

    authors: Deuse T,Hua X,Wang D,Maegdefessel L,Heeren J,Scheja L,Bolaños JP,Rakovic A,Spin JM,Stubbendorff M,Ikeno F,Länger F,Zeller T,Schulte-Uentrop L,Stoehr A,Itagaki R,Haddad F,Eschenhagen T,Blankenberg S,Kiefmann R,Reic

    更新日期:2014-05-29 00:00:00

  • Molybdenum chloride catalysts for Z-selective olefin metathesis reactions.

    abstract::The development of catalyst-controlled stereoselective olefin metathesis processes has been a pivotal recent advance in chemistry. The incorporation of appropriate ligands within complexes based on molybdenum, tungsten and ruthenium has led to reactivity and selectivity levels that were previously inaccessible. Here w...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature21043

    authors: Koh MJ,Nguyen TT,Lam JK,Torker S,Hyvl J,Schrock RR,Hoveyda AH

    更新日期:2017-02-02 00:00:00

  • How sure are you of your result? Put a number on it.

    abstract:: ...

    journal_title:Nature

    pub_type: 新闻

    doi:10.1038/d41586-018-07589-2

    authors: Goodman SN

    更新日期:2018-12-01 00:00:00

  • Frizzled-7 signalling controls tissue separation during Xenopus gastrulation.

    abstract::Cell signalling through Frizzled receptors has evolved to considerable complexity within the metazoans. The Frizzled-dependent signalling cascade comprises several branches, whose differential activation depends on specific Wnt ligands, Frizzled receptor isoforms and the cellular context. In Xenopus laevis embryos, th...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35101621

    authors: Winklbauer R,Medina A,Swain RK,Steinbeisser H

    更新日期:2001-10-25 00:00:00