Abstract:
:Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing technique magic-angle spinning dynamic nuclear polarization (MAS-DNP) have substantially expanded the territory of ssNMR investigations and enabled the detection of polymer interfaces in a cellular environment. This article highlights the emerging MAS-DNP approaches and their applications to the analysis of biomolecular composites and intact cells to determine the folding pathway and ligand binding of proteins, the structural polymorphism of low-populated biopolymers, as well as the physical interactions between carbohydrates, proteins, and lignin. These structural features provide an atomic-level understanding of many cellular processes, promoting the development of better biomaterials and inhibitors. It is anticipated that the capabilities of MAS-DNP in biomolecular and biomaterial research will be further enlarged by the rapid development of instrumentation and methodology.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Chakraborty A,Deligey F,Quach J,Mentink-Vigier F,Wang P,Wang Tdoi
10.1042/BST20191084subject
Has Abstractpub_date
2020-06-30 00:00:00pages
1089-1099issue
3eissn
0300-5127issn
1470-8752pii
223847journal_volume
48pub_type
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