Abstract:
:Guinea-pigs were chronically treated with morphine by subcutaneous implantation of a compounded morphine pellet. After 7 days, the animals were sacrificed, and the small intestine was removed and extracted for the assay of 6 neuropeptides (substance P, vasoactive intestinal peptide, galanin, dynorphin A (1-8), dynorphin A (1-17) and alpha-neo-endorphin). The neuropeptide content of the extracts was measured by radioimmunoassay, and the extracts were subjected to analysis by reversed-phase high pressure liquid chromatography. The measured level of each neuropeptide was not significantly different from that in a control group of animals, and the chromatographic elution pattern obtained for each peptide was also similar in control and morphine-treated animals. Furthermore, the major peak of immunoreactivity for each peptide occurred with approximately the same retention time as synthetic standards having the porcine sequence for that peptide. It is concluded that chronic morphine treatment does not alter the tissue concentration or molecular form of at least 5 of the 6 neuropeptides studied. In the case of a alpha-neo-endorphin, a peak of immunoreactivity was discovered that did not correspond to the synthetic peptide. The amount of immunoreactive material present in this peak varied with oxidation and so its contribution to the assay results could not be calculated.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Shuttleworth CW,Murphy Rdoi
10.1016/0143-4179(88)90063-7subject
Has Abstractpub_date
1988-11-01 00:00:00pages
249-56issue
4eissn
0143-4179issn
1532-2785pii
0143-4179(88)90063-7journal_volume
12pub_type
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