High-throughput cell and spheroid mechanics in virtual fluidic channels.

Abstract:

:Microfluidics by soft lithography has proven to be of key importance for biophysics and life science research. While being based on replicating structures of a master mold using benchtop devices, design modifications are time consuming and require sophisticated cleanroom equipment. Here, we introduce virtual fluidic channels as a flexible and robust alternative to microfluidic devices made by soft lithography. Virtual channels are liquid-bound fluidic systems that can be created in glass cuvettes and tailored in three dimensions within seconds for rheological studies on a wide size range of biological samples. We demonstrate that the liquid-liquid interface imposes a hydrodynamic stress on confined samples, and the resulting strain can be used to calculate rheological parameters from simple linear models. In proof-of-principle experiments, we perform high-throughput rheology inside a flow cytometer cuvette and show the Young's modulus of isolated cells exceeds the one of the corresponding tissue by one order of magnitude.

journal_name

Nat Commun

journal_title

Nature communications

authors

Panhwar MH,Czerwinski F,Dabbiru VAS,Komaragiri Y,Fregin B,Biedenweg D,Nestler P,Pires RH,Otto O

doi

10.1038/s41467-020-15813-9

subject

Has Abstract

pub_date

2020-05-04 00:00:00

pages

2190

issue

1

issn

2041-1723

pii

10.1038/s41467-020-15813-9

journal_volume

11

pub_type

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