Abstract:
:Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Karpf S,Riche CT,Di Carlo D,Goel A,Zeiger WA,Suresh A,Portera-Cailliau C,Jalali Bdoi
10.1038/s41467-020-15618-wsubject
Has Abstractpub_date
2020-04-28 00:00:00pages
2062issue
1issn
2041-1723pii
10.1038/s41467-020-15618-wjournal_volume
11pub_type
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