Abstract:
:The main goal of peri-implantitis treatment is to control infection and arrest bone loss, which requires the removal of polymicrobial biofilms on the implant surface and the reduction of tissue invasion. Additionally, prognosis can be improved if reosseointegration occurs on previously contaminated implants. To evaluate whether graphene oxide (GO) can remove polymicrobial biofilms, biofilms were established on titanium surfaces in vitro and treated with different methods: group B, removed only with brushing; group G, treated with different GO concentrations (64, 128, 256, and 512 μg/mL); group GB, combined treatments of groups B and G; and group C, untreated. Subsequently, to evaluate reosteogenesis on previously contaminated titanium, 4 groups were used: groups C, B, GB-256, and GB-512 (treated with 256 and 512 μg/mL of GO, respectively). Intact clean titanium (IC) was used as a control. Additionally, cell behavior on IC treated with GB-256 (IGB-256) and GB-512 (IGB-512) was compared with that of the GB-256 and GB-512 groups, respectively. The results showed that at high concentrations (≥256 μg/mL), GO eliminated residual bacteria and inhibited biofilm reformation after brushing, whereas neither GO nor brushing alone could achieve this. Bone marrow-derived mesenchymal stem cell viability in groups GB-256 and IC was higher than that in groups GB-512, C, and B (P < 0.05). No significant difference was found between group GB-256 and group IC (P > 0.05). Osteogenic differentiation of bone marrow-derived mesenchymal stem cells in group GB-256 was higher than that in groups IC, GB-512, C, and B. No difference was found between groups IGB-256 and IGB-512 and groups GB-256 and GB-512, respectively (P > 0.05). In conclusion, 256 μg/mL of GO combined with brushing significantly removed polymicrobial biofilms that remained on the previously contaminated titanium surfaces. The bone marrow-derived mesenchymal stem cell osteogenic potential was regained or even enhanced on the titanium surfaces treated this way in vitro, which might provide a new idea for treating peri-implantitis.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Qin W,Wang C,Jiang C,Sun J,Yu C,Jiao Tdoi
10.1177/0022034520913873subject
Has Abstractpub_date
2020-07-01 00:00:00pages
922-929issue
8eissn
0022-0345issn
1544-0591journal_volume
99pub_type
杂志文章abstract::Although a secular decline is being seen in coronal caries in children in industrialized countries, there appears to be a relatively high prevalence of root caries, with chronologically older adults exhibiting the highest prevalences. This paper reviews the descriptive and analytical epidemiology of root caries as fou...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345900690051901
更新日期:1990-05-01 00:00:00
abstract::Rat parotid secretory granule membranes were examined for the presence of calcium-dependent protein kinase activities and kinase substrates. Protein kinase C (C-kinase), which is stimulated by certain phospholipids, was present in the membranes, as indicated by its ability to catalyze the phosphorylation of histone. T...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660022901
更新日期:1987-02-01 00:00:00
abstract::Six subjects wore intra-oral devices carrying eight bovine enamel blocks which were covered with a layer of S. mutans. They rinsed their mouths for five sec, one min, or multiples of one min with a 5 or 10% glucose (G) solution. Demineralization was measured after 45 min by determining the change in iodide permeabilit...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345860650021001
更新日期:1986-02-01 00:00:00
abstract::Teas varying in fluoride and tannin concentration were evaluated in rats for anticariogenic activity. There was a direct correlation between fluoride in tea and the inhibition of sulcal caries in rats, whereas no relationship was observed between tannin and this type of lesion. Teas also had a significant effect on ca...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630051001
更新日期:1984-05-01 00:00:00
abstract::Wall-to-wall (WTW) polymerization contraction of filled and unfilled chemically and photo-initiated resins was studied in relation to the WTW distance. In an experimental set-up, the resins were bonded to two opposing disks, and the axial (WTW) displacement resulting from the polymerization shrinkage was measured cont...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680010701
更新日期:1989-01-01 00:00:00
abstract::Composite resin restorations in cavities are generally associated with the development of a contraction gap during the first minutes after insertion. Enamel-Bond-Concise composite restoration were placed on both approximal sides of extracted pre-molars. The cervical walls of all cavities extended beyond the enamel-cem...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630051501
更新日期:1984-05-01 00:00:00
abstract::Dental caries can be described as a dysbiosis of the oral microbial community, in which acidogenic, aciduric, and acid-adapted bacterial species promote a pathogenic environment, leading to demineralization. Alkali generation by oral microbes, specifically via arginine catabolic pathways, is an essential factor in mai...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517707512
更新日期:2017-07-01 00:00:00
abstract::Silver cations generated by passing low intensity direct current through pure silver electrodes were found to be sufficiently antibacterial to cause sterilization of samples of infected dentin. The optimal procedure involved a 5 microA current applied for 20 minutes with the anode then left in contact with the sample....
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570091901
更新日期:1978-09-01 00:00:00
abstract::Much research has been devoted to the study of etched enamel, since it is critical to bonding. Currently, there are no precise data regarding the etched-enamel specific surface area. The aim of this study was to characterize, by two different methods, the surface of human dental enamel in vitro after being etched. It ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700607
更新日期:2008-06-01 00:00:00
abstract::A series of in vitro studies have been performed to study the release of tin and fluoride from an experimental prophylactic cup and the influence of this release on the fluoride content of enamel. It has been found that the release of fluoride from the prophy cup during a simulated prophylaxis results in a significant...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345760550030801
更新日期:1976-05-01 00:00:00
abstract::Gingival overgrowth is a common side-effect of the administration of cyclosporin A (CSA), phenytoin, and calcium blockers. To identify the signaling mechanisms possibly involved in the overgrowth, we examined how CSA affects the activities of MAP kinases and transcription factors in human gingival fibroblasts (HGF). T...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910508400609
更新日期:2005-06-01 00:00:00
abstract::In a previous experiment, we found that masseter muscle spindles show functional plasticity after 5 to 15 days under increased occlusal vertical dimension (iOVD). In the present study, we hypothesized that spindle function would eventually recover if longer observation periods were allowed. Therefore, in this study we...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500914
更新日期:2006-09-01 00:00:00
abstract::Priming with tumor antigens is one of the most important strategies in cancer immunotherapy. To enhance tumor antigenicity, OK-432, a streptococcal preparation, was coupled to squamous cell carcinoma (KLN-205) by means of a 0.2% glutaraldehyde method. The purpose of this study was to investigate whether OK-432-conjuga...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910308200813
更新日期:2003-08-01 00:00:00
abstract::The monomer triethyleneglycoldimethacrylate (TEGDMA) is used as a diluent in many resin-based dental materials. It was previously shown in vitro that TEGDMA was released into the adjacent biophase from such materials during the first days after placement. In this study, the uptake, distribution, and excretion of 14C-T...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800050501
更新日期:2001-05-01 00:00:00
abstract::Tritiated proline was administered to domestic cats during the development of their permanent dentition. The amount of residual radioactivity was determined autoradiographically at various times after cavity preparation in mature molars and premolars. Two wk after cavity preparation, the amount of radiolabel beneath t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345820610090101
更新日期:1982-09-01 00:00:00
abstract::Teeth develop through distinct morphological stages. At the cap stage, a compactly clustered and concentrically arranged cell mass, the enamel knot, appears at the tip of the enamel organ. Cells in this knot express sets of key molecules, and as such have been proposed to act as a signaling center directing tooth morp...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514525784
更新日期:2014-05-01 00:00:00
abstract::Macrophage colony-stimulating factor (CSF-1) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-1. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-1 in these cells. We determined the effect of PDGF on CSF-1 expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700105
更新日期:2008-01-01 00:00:00
abstract::Candida albicans is an opportunistic fungal organism frequently detected in the oral cavity of children with severe early childhood caries (S-ECC). Previous studies suggested the cariogenic potential of C. albicans, in vitro and in vivo, and further demonstrated its synergistic interactions with Streptococcus mutans. ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518790941
更新日期:2018-12-01 00:00:00
abstract::Sensitivity or pain of the mucoperiosteum covering the mandibular edentulous ridge is often thought to limit bite forces in complete-denture wearers. Therefore, bite forces with mandibular implant-retained overdentures may depend on the degree of implant support. This study analyzed the effects of different degrees of...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1177/00220345980770101101
更新日期:1998-10-01 00:00:00
abstract::The objective of this study was to determine the in vitro corrosion products that resulted from crevice corrosion of low- and high-copper dental amalgams. Specimens were potentiostatically polarized in a chloride-containing electrolyte while set against a PTFE surface to form a crevice. After 16 h, corrosion products ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345910700071301
更新日期:1991-07-01 00:00:00
abstract::Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein label and c-, e-rhodam...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/002203457605500126011
更新日期:1976-01-01 00:00:00
abstract::Recent advances in molecular biology have facilitated analyses of the oral microbiome ("Who are they?"); however, its functions (e.g., metabolic activities) are poorly understood ("What are they doing?"). This review aims to summarize our current understanding of the metabolism of the oral microbiome. Saccharolytic ba...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034515606045
更新日期:2015-12-01 00:00:00
abstract::The aim of this study was to compare the effectiveness of dexamethasone administration following arthrocentesis of the temporomandibular joint (TMJ) with a placebo (saline). Twenty-eight participants with TMJ arthralgia were randomly assigned to two groups of a parallel double-blind RCT. In both groups, an arthrocente...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034511431260
更新日期:2012-02-01 00:00:00
abstract::During amelogenesis in the rat incisor, modulating ruffle-ended (RA) and smooth-ended (SA) ameloblasts are distributed as bands in the enamel organ of the maturation zone. This distribution of the two cell types has been shown to be precisely correlated with a banding of the underlying enamel, as shown by staining and...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680020101
更新日期:1989-02-01 00:00:00
abstract::Several studies indicated that serotype/biotype c strains are the most common on a global basis, but that regional differences may occur relative to other serotype/biotypes. Of particular interest is the observation that individuals residing in the Middle East have higher incidences of serotype e and d strains relativ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630011201
更新日期:1984-01-01 00:00:00
abstract::A three-month study of the chemistry of the water erosion of two forms of ASPA cement has been made. The effect of varying cement consistency and cure time was investigated. The results are discussed in terms of the known chemistry and structure of the cement. The erosion behavior is compared to that of silicate, sili...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345760550060501
更新日期:1976-11-01 00:00:00
abstract::We investigated whether oral health, represented by missing teeth, was associated with an increased risk of cardiovascular disease, including myocardial infarction (MI), heart failure (HF), stroke, and all-cause mortality. Subjects who underwent routine dental examinations and health checkups provided by the Korean Na...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518814829
更新日期:2019-02-01 00:00:00
abstract::Microducers and modified cephalometric roentgenogram techniques were used to study maximum biting forces and moments in an adult male. The force at the second molar was approximately 10% greater than that at the first molar. At the second molar, the moment of force was approximately 15% less than its value at the firs...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345750540010401
更新日期:1975-01-01 00:00:00
abstract::For restoring cavitated dental lesions, whether carious or not, a large number of material combinations are available. We aimed to systematically review and synthesize data of comparative dental restorative trials. A systematic review was performed. Randomized controlled trials published between 2005 and 2015 were inc...
journal_title:Journal of dental research
pub_type: 杂志文章,meta分析,评审
doi:10.1177/0022034516631285
更新日期:2016-06-01 00:00:00
abstract::The influence of the molecular weight of the poly(acrylic acid) component on some properties of glass-ionomer cement has been investigated. The results can be explained by treatment of glass-ionomer cements as thermoplastic composites. Many of the concepts of polymer science can be applied successfully in a qualitativ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680021401
更新日期:1989-02-01 00:00:00