Abstract:
BACKGROUND:Drug resistant tuberculosis poses a great challenge for tuberculosis control worldwide. Timely determination of drug resistance and effective individual treatment are essential for blocking the transmission of drug resistant Mycobacterium tuberculosis. We aimed to establish and evaluate the accuracy of a reverse dot blot hybridization (RDBH) assay to simultaneously detect the resistance of four anti-tuberculosis drugs in M. tuberculosis isolated in China. METHODS:In this study, we applied a RDBH assay to simultaneously detect the resistance of rifampicin (RIF), isoniazid (INH), streptomycin (SM) and ethambutol (EMB) in 320 clinical M. tuberculosis isolates and compared the results to that from phenotypic drug susceptibility testing (DST) and sequencing. The RDBH assay was designed to test up to 42 samples at a time. Pearson's chi-square test was used to compute the statistical measures of the RDBH assay using the phenotypic DST or sequencing as the gold standard method, and Kappa identity test was used to determine the consistency between the RDBH assay and the phenotypic DST or sequencing. RESULTS:The results showed that the concordances between phenotypic DST and RDBH assay were 95% for RIF, 92.8% for INH, 84.7% for SM, 77.2% for EMB and the concordances between sequencing and RDBH assay were 97.8% for RIF, 98.8% for INH, 99.1% for SM, 93.4% for EMB. Compared to the phenotypic DST results, the sensitivity and specificity of the RDBH assay for resistance detection were 92.4 and 98.5% for RIF, 90.3 and 97.3% for INH, 77.4 and 91.5% for SM, 61.4 and 85.7% for EMB, respectively; compared to sequencing, the sensitivity and specificity of the RDBH assay were 97.7 and 97.9% for RIF, 97.9 and 100.0% for INH, 97.8 and 100.0% for SM, 82.6 and 99.1% for EMB, respectively. The turnaround time of the RDBH assay was 7 h for testing 42 samples. CONCLUSIONS:Our data suggested that the RDBH assay could serve as a rapid and efficient method for testing the resistance of M. tuberculosis against RIF, INH, SM and EMB, enabling early administration of appropriate treatment regimens to the affected drug resistant tuberculosis patients.
journal_name
Infect Dis Povertyjournal_title
Infectious diseases of povertyauthors
Wan L,Guo Q,Wei JH,Liu HC,Li MC,Jiang Y,Zhao LL,Zhao XQ,Liu ZG,Wan KL,Li GL,Guan CXdoi
10.1186/s40249-020-00652-zsubject
Has Abstractpub_date
2020-04-16 00:00:00pages
38issue
1eissn
2095-5162issn
2049-9957pii
10.1186/s40249-020-00652-zjournal_volume
9pub_type
杂志文章abstract:BACKGROUND:A recent study in the Logo and Rethy health zones in the Ituri Province in the Democratic Republic of Congo (DRC) reported that the majority of the persons with epilepsy (PWE) had not been treated with anti-epileptic medication (AEM) or had stopped treatment. Prior to the implementation of an epilepsy treatm...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-018-0498-0
更新日期:2018-12-04 00:00:00
abstract:BACKGROUND:Neglected tropical diseases (NTDs) prevail in conditions of poverty and contribute to the maintenance of social inequality. Out of the NTDs prioritized by the Brazilian Ministry of Health, four parasitic infections require mandatory notification: acute Chagas disease, leishmaniasis, malaria, and schistosomia...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0369-0
更新日期:2017-11-03 00:00:00
abstract:BACKGROUND:Malaria surveillance system strengthening is essential in the progress towards malaria elimination. In Nigeria, more attention is being given to this recently as the country is striving towards achieving elimination. However, the surveillance system performance is fraught with challenges including poor data ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-020-0629-2
更新日期:2020-02-10 00:00:00
abstract:BACKGROUND:Men who have sex with men (MSM) has become the group with the fastest growing HIV epidemic in China. Since many Chinese MSM are conducting HIV self-testing, we aimed to determine the rate of HIV care seeking after self-testing, examine characteristics of "seekers" compared to "non-seekers," and explore facto...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0326-y
更新日期:2017-06-28 00:00:00
abstract:BACKGROUND:A high epilepsy prevalence has been reported in several onchocerciasis-endemic villages along the Mbam and Sanaga river valleys in Cameroon, including Bilomo and Kelleng. We sought to determine the prevalence of epilepsy in these two villages following more than 13 years of community-directed treatment with ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-018-0497-1
更新日期:2018-12-03 00:00:00
abstract:BACKGROUND:Interventions are currently being used against 'infectious diseases of poverty', which remain highly debilitating and deadly in most endemic countries, especially malaria, schistosomiasis, echinococcosis and African sleeping sickness. However, major limitations of current 'traditional' methods for diagnosis ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0344-9
更新日期:2017-10-02 00:00:00
abstract:BACKGROUND:Zooprophylaxis is the use of wild or domestic animals, which are not the reservoir host of a given disease, to divert the blood-seeking malaria vectors from human hosts. In this paper, we systematically reviewed zooprophylaxis to assess its efficacy as a malaria control strategy and to evaluate the possible ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,评审
doi:10.1186/s40249-017-0366-3
更新日期:2017-10-25 00:00:00
abstract:BACKGROUND:Hand, foot, and mouth disease (HFMD) has become an emerging infectious disease in China in the last decade. There has been evidence that meteorological factors can influence the HFMD incidence, and understanding the mechanisms can help prevent and control HFMD. METHODS:HFMD incidence data and meteorological...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-018-0388-5
更新日期:2018-01-31 00:00:00
abstract:BACKGROUND:Ethiopia is one of the African countries where Plasmodium falciparum and P. vivax co-exist. Monitoring and evaluation of current malaria transmission status is an important component of malaria control as it is a measure of the success of ongoing interventions and guides the planning of future control and el...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,评审
doi:10.1186/s40249-018-0487-3
更新日期:2018-11-05 00:00:00
abstract:BACKGROUND:Aedes borne viral diseases, notably dengue, are increasingly reported in Cameroon with Aedes aegypti being a major vector. Data on insecticide resistance of this vector and underlying mechanisms needed for outbreak preparedness remain scarce in Cameroon. Here, we present the nationwide distribution of insect...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-020-00769-1
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings, sensitive tools are required to monitor infection status to prevent potential re-emergence. In Rahala, where transmission cycle of Schistosoma haematobium is interrupted since 2004 but where 30% of s...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0289-z
更新日期:2017-04-06 00:00:00
abstract:BACKGROUND:Swaziland has the highest national incidence of tuberculosis (TB) in the world, with treatment success rates well below the 85 % international target. Treatment support as part of comprehensive TB services is a core component of the Stop TB Strategy. This study investigated the effects of financial incentive...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-015-0059-8
更新日期:2015-06-05 00:00:00
abstract:BACKGROUND:Epidermal parasitic skin diseases (EPSD) occur in most countries and cause a considerable health and economic burden, particularly in the tropics and subtropics. The aim of this study was to assess and analyse peer-reviewed literature on EPSD in humans. The results of this study serve as an indicator of the ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-018-0456-x
更新日期:2018-08-06 00:00:00
abstract::The transmission of infectious diseases is a dynamic process determined by multiple factors originating from disease pathogens and/or parasites, vector species, and human populations. These factors interact with each other and demonstrate the intrinsic mechanisms of the disease transmission temporally, spatially, and ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0339-6
更新日期:2017-09-11 00:00:00
abstract:BACKGROUND:The livestock industry plays a vital role in the economy of Nigeria. It serves as a major source of income and livelihood for majority of Nigerians who are rural settlers and contributes about 5.2% to the National Gross Domestic Product (GDP). Helminths however, cause economic losses due to reductions in mil...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,meta分析
doi:10.1186/s40249-018-0438-z
更新日期:2018-05-29 00:00:00
abstract::Mechanisms and outcomes of host-parasite interactions during malaria co-infections with gastrointestinal helminths are reasonably understood. In contrast, very little is known about such mechanisms in cases of malaria co-infections with tissue-dwelling parasites. This is lack of knowledge is exacerbated by misdiagnosi...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s40249-015-0070-0
更新日期:2015-08-29 00:00:00
abstract:BACKGROUND:National tuberculosis (TB) programs increasingly engage with international non-governmental organizations (INGOs), especially to provide TB care in complex settings where community involvement might be required. In Myanmar, however, there is limited data on how such INGO community-based programs are organize...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0285-3
更新日期:2017-03-24 00:00:00
abstract:BACKGROUND:In order to achieve the goal of malaria elimination, the Chinese government launched the National Malaria Elimination Programme in 2010. However, as a result of increasing cross-border population movements, the risk of imported malaria cases still exists at the border areas of China, resulting in a potential...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0322-2
更新日期:2017-07-06 00:00:00
abstract:BACKGROUND:The People's Republic of China (P. R. China) has made significant progress on schistosomiasis control. Among the 12 provincial-level administrative divisions (PLADs) with schistosomiasis endemic in P. R. China, Guangdong, Shanghai, Fujian, Guangxi and Zhejiang PLADs (following as five PLADs) had successively...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-020-00758-4
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Access to clean and safe drinking water that is free from pathogenic protozoan parasites, especially Cryptosporidium parvum and Giardia lamblia that cause gastrointestinal illness in humans, is still an issue in Southeast Asia (SEA). This study is the first attempt to detect the aforementioned protozoan para...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-016-0095-z
更新日期:2016-01-13 00:00:00
abstract::Schistosomiasis is an important zoonotic parasitic disease that causes serious harms to humans and animals. Surveillance and diagnosis play key roles in schistosomiasis control, however, current techniques for surveillance and diagnosis of the disease have limitations. As genome data for parasites are increasing, nove...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,评审
doi:10.1186/s40249-016-0116-y
更新日期:2016-03-30 00:00:00
abstract:BACKGROUND:Health workers (HWs) are at increased risk for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection and a possible source of nosocomial transmission clusters. Despite the increased risk, the best surveillance strategy and management of exposed HWs are not yet well known. The aim of this rev...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,评审
doi:10.1186/s40249-020-00756-6
更新日期:2020-10-07 00:00:00
abstract:BACKGROUND:Hepatitis A (HepA) vaccination and economic transitions can change the epidemiology of HepA. China's Gross Domestic Product (GDP) per capita was known to be inversely associated with the incidence of HepA, but a deeper understanding of the epidemiology of HepA in different socio-economic regions is lacking. ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-019-0591-z
更新日期:2019-10-03 00:00:00
abstract:BACKGROUND:Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0251-0
更新日期:2017-04-04 00:00:00
abstract:CORRECTION:After publication of this article [1] it came to our attention that the affiliation of Jun Chen and Hong-zhou Lu were incorrectly shown.Jun Chen's affiliation should have been given as Department of Infectious Diseases, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.Hong-zhou Lu sh...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,已发布勘误
doi:10.1186/s40249-017-0376-1
更新日期:2017-11-23 00:00:00
abstract:BACKGROUND:The recent development of rapid diagnostic tests (RDTs) for human African trypanosomiasis (HAT) enables elimination programmes to decentralise serological screening services to frontline health facilities. However, patients must still undertake multiple onwards referral steps to either be confirmed or discou...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-018-0472-x
更新日期:2018-08-18 00:00:00
abstract:BACKGROUND:Malaria is a major cause of morbidity and mortality in Northeast India. As there is limited information available on the potential influence of socio-economic variables on malaria risk, the present study was conducted to assess the influence of demographic factors, the socio-economic status, and knowledge, a...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/2049-9957-3-19
更新日期:2014-06-24 00:00:00
abstract:BACKGROUND:It is one of the most infamous quotes in the history of biomedicine: "It is time to close the book on infectious diseases, and declare the war against pestilence won." Long attributed to the United States Surgeon General, Dr. William H. Stewart (1965-1969), the statement is frequently used as a foil by scien...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/2049-9957-2-3
更新日期:2013-02-18 00:00:00
abstract::Unfortunately, the original article [1] contained some errors. The table title of Tables 4, 5, 6, 7 were interchanged by mistake and displayed incorrectly in the article. The correct table titles of Tables 4, 5, 6, 7 can be found below. ...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章,已发布勘误
doi:10.1186/s40249-018-0415-6
更新日期:2018-04-20 00:00:00
abstract:BACKGROUND:Before 2014 (the year of closure of the two largest needle exchange programs in Hungary, which halved the number of available syringes in the country despite increased injecting risk practices) no HIV was reportedly acquired in Hungary among people who inject drugs (PWIDs) who were not also men who had sex w...
journal_title:Infectious diseases of poverty
pub_type: 杂志文章
doi:10.1186/s40249-017-0360-9
更新日期:2017-10-11 00:00:00