Abstract：
:Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non-invasive method for obtaining a blood sample (cf. cannulation and cardiac puncture). Here, a general set of recommendations are provided for optimizing the blood sampling protocol that reflects best practices in animal welfare and sample integrity. This includes selecting appropriate use of anaesthetics for blood sampling as well as restraint techniques for situations where sedation is not used. In addition, ideal sampling environments where the fish can freely ventilate and strategies for minimizing handling time are discussed. This study summarizes the techniques used for extracting blood from the caudal vasculature in live fishes, highlighting the phlebotomy itself, the timing of sampling events and acceptable blood sample volumes. This study further discuss considerations for selecting appropriate physiological metrics when sampling in the caudal region and the potential benefits that this technique provides with respect to long-term biological assessments. Although general guidelines for blood sampling are provided here, it should be recognized that contextual considerations (e.g., taxonomic diversity, legal matters, environmental constraints) may influence the approach to blood sampling. Overall, it can be concluded that when done properly, blood sampling live fishes through the caudal vasculature is quick, efficient and minimally invasive, thus promoting conditions where live release of focal animals is possible.
journal_name
J Fish Bioljournal_title
Journal of fish biologyauthors
Lawrence MJ,Raby GD,Teffer AK,Jeffries KM,Danylchuk AJ,Eliason EJ,Hasler CT,Clark TD,Cooke SJdoi
10.1111/jfb.14339subject
Has Abstractpub_date
2020-07-01 00:00:00pages
4-15issue
1eissn
0022-1112issn
1095-8649journal_volume
97pub_type
杂志文章,评审abstract：:A scavenging interaction between the arrow tooth eel Synaphobranchus kaupii and the Portuguese dogfish Centroscymnus coelolepis, both ubiquitous components of fish assemblages at bathyal depths, was observed. Using a baited camera between 1297 and 2453 m in the eastern Atlantic Ocean continental slope, it was shown th...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2011.03014.x
更新日期：2011-07-01 00:00:00
abstract：:The effect of UV radiation on habitat use of two species of intertidal fishes that inhabit the same pools but exhibit different activity levels and diets was measured: the highly active omnivorous Girella laevifrons and the cryptic carnivorous Graus nigra. Individuals of each species were acclimated to a tank divided ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12566
更新日期：2015-02-01 00:00:00
abstract：:A new brain-cell line derived from Japanese flounder Paralichthys olivaceus (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid-like. Chromosome analysis revealed the cell line to possess the normal P. olivaceus diploid karyotype of 2n...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12700
更新日期：2015-07-01 00:00:00
abstract：:Acute thermal and asphyxia exposure risks for freshwater fish occupying three tropical wetland typologies were examined. Field water-quality data revealed that fish in wetlands grazed by pigs had the highest exposure risks, because they are shallow and heavily damaged by pig activities. In contrast, with the exception...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13742
更新日期：2018-10-01 00:00:00
abstract：:This study investigated the relationship between spatial variations in predation risk and abundance of northern redbelly dace Phoxinus eos at both macroscale (littoral v. pelagic zones) and microscale (structured v. open water habitats in the littoral zone) of Canadian Shield lakes. Minnow traps were placed in both st...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2009.02183.x
更新日期：2009-05-01 00:00:00
abstract：:The growth, physiology and skin pigmentation of pacamã Lophiosilurus alexandri juveniles were evaluated in an experiment using different tank colours (white, yellow, green, blue, brown and black) over an 80 day period. The tank colours did not cause significant differences to final body mass, total length, survival ra...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13208
更新日期：2017-03-01 00:00:00
abstract：:The field data from four humic lakes suggested that water colour may have both direct and indirect effects on inter- and intra-specific interactions of perch Perca fluviatilis and roach Rutilus rutilus. The results agree with suggestions that, compared with R. rutilus, P. fluviatilis may be an inferior forager on zoop...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2010.02682.x
更新日期：2010-07-01 00:00:00
abstract：:Wild, downstream-migrating cutthroat trout, Oncorhynchus clarkii clarkii, smolts and adults were captured at a weir in Big Beef Creek, Hood Canal, Washington, surgically implanted with acoustic tags and tracked to identify spring and summer movements using stationary receivers in order to test the assumption that the ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12209
更新日期：2013-11-01 00:00:00
abstract：:The influence of the lunar cycle on prey availability, diet shifts and overlap between larval Anchovia clupeoides and Cetengraulis edentulus was evaluated in mangrove creeks of the Goiana Estuary. Copepod eggs were highly abundant in the first and last quarter, at the full moon and zoea of Ucides cordatus (Ocypodidae)...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13121
更新日期：2016-10-01 00:00:00
abstract：:Migration behaviour and estuarine mortality of cultivated Atlantic salmon Salmo salar smolts in a 16 km long estuary were studied using two methods: (1) acoustic telemetry and (2) group tagging in combination with trap nets. Progression rates of surviving individuals through the estuary were relatively slow using both...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13097
更新日期：2016-10-01 00:00:00
abstract：:A genotyping assay for the Ion Torrent Ion PGM platform was developed for fast and cost-effective targeted genotyping of key single nucleotide polymorphisms (SNPs) earlier identified using a genome-wide SNP array in Atlantic salmon Salmo salar. The method comprised a simple primer design step for multiplex-polymerase ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13149
更新日期：2016-12-01 00:00:00
abstract：:The numbers of different types of ovarian follicles (developing, degenerating and postovulatory follicles) were estimated in bluefin tuna Thunnus thynnus using two stereological procedures: the model-based method of Weibel & Gomez, which has become a tool of broad application in the quantification of oocytes in fishes...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2010.02713.x
更新日期：2010-08-01 00:00:00
abstract：:The diet of pearly razorfish Xyrichtys novacula, caught monthly along the shores of the Island of Lampedusa, appeared to be mainly composed of crustaceans, followed by colonial ascidians, molluscs and polychaetes. Among prey, sand dwellers and phanerogam-associated species were recorded. In winter months, the diet was...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2010.02599.x
更新日期：2010-05-01 00:00:00
abstract：:Accelerometer technology was used to evaluate behaviours in the teleost ambush predator pike Esox lucius foraging on crucian carp Carassius carassius. Automated rule-based estimates of prey-size determined handling time were obtained and are compared with video-recorded behaviours. Solutions to tag attachment and the ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13310
更新日期：2017-06-01 00:00:00
abstract：:Life-history variables for three incidentally captured species of seahorse (Kellogg's seahorse Hippocampus kelloggi, the hedgehog seahorse Hippocampus spinosissimus and the three-spot seahorse Hippocampus trimaculatus) were established using specimens obtained from 33 fisheries landing sites in Peninsular Malaysia. Wh...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12527
更新日期：2015-01-01 00:00:00
abstract：:The effect of flume length and impingement time on post-exercise lactate concentrations in brook charr Salvelinus fontinalis were examined. Swimming in longer flumes increased lactate concentrations, as does impingement after swimming in short flumes. ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2009.02513.x
更新日期：2010-02-01 00:00:00
abstract：:The length and mass ratio, diet and isotopic composition of Aplochiton zebra and Aplochiton taeniatus inhabiting a Salmo trutta-invaded and a S. trutta-free lake in southern Patagonia were compared. Results indicate that S. trutta exercises important trophic interference over A. zebra and A. taeniatus, causing changes...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2012.03489.x
更新日期：2013-02-01 00:00:00
abstract：:The age, growth and maturity of bonnetheads Sphyrna tiburo inhabiting the estuarine and coastal waters of the western North Atlantic Ocean (WNA) from Onslow Bay, North Carolina, south to West Palm Beach, Florida, were examined. Vertebrae were collected and aged from 329 females and 217 males ranging in size from 262 t...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12450
更新日期：2014-09-01 00:00:00
abstract：:It was hypothesized that the structural heterogeneity provided by submerged trees positively favours the spatial distribution of fish abundance at early stages of development in an area under the influence of a Neotropical reservoir in the Paraná River basin. The distribution at early stages of development of the most...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12966
更新日期：2016-07-01 00:00:00
abstract：:Morphological (growth, Fulton's condition factor), physiological (per cent dry mass, total lipid content) and behavioural (activity levels) response patterns of carp gudgeon Hypseleotris spp. were examined in response to food deprivation during a 56 day experiment. Considerable variability in the nature and magnitude ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2011.03156.x
更新日期：2012-01-01 00:00:00
abstract：:An analysis of 107 individuals of Atlantic hookear sculpin Artediellus atlanticus from the Barents Sea-Svalbard region and from north-east Greenland shows that the state of the parietal spines, although not randomly distributed geographically, occurs sympatrically. They do not diagnostically describe populations. They...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/j.1095-8649.2012.03484.x
更新日期：2013-01-01 00:00:00
abstract：:Fish reproduction is likely to be affected by increasing water temperatures arising from climate change. Normal changes in environmental temperature have the capacity to affect endocrine function and either advance or retard gametogenesis and maturation, but above-normal temperatures have deleterious effects on reprod...
journal_title：Journal of fish biology
pub_type： 杂志文章,评审
doi：10.1111/j.1095-8649.2009.02484.x
更新日期：2010-01-01 00:00:00
abstract：:A specimen of angular roughshark Oxynotus centrina has been kept successfully in captivity for the first time. Over a period of 24 months, the specimen preyed exclusively on the contents of elasmobranch egg cases, suggesting a specialized trophic niche. ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12761
更新日期：2015-10-01 00:00:00
abstract：:Deania calcea (n = 420) were collected from the catch of deep-water trawlers in the southern and eastern scalefish and shark fishery in southern Australia during the years 2008-2011. The total length (LT ) range varied between sexes, females being larger (n = 264; 280-1530 mm) than males (n = 156; 310-921 mm). The rep...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12756
更新日期：2015-09-01 00:00:00
abstract：:The large size, high trophic level and wide distribution of Hexanchiformes (cow and frilled sharks) should position this order as important apex predators in coastal and deep-water ecosystems. This review synthesizes available information on Hexanchiformes, including information not yet published, with the purpose of ...
journal_title：Journal of fish biology
pub_type： 杂志文章,评审
doi：10.1111/j.1095-8649.2012.03242.x
更新日期：2012-04-01 00:00:00
abstract：:This study examined how colour varies across season and sex in the fantail darter Etheostoma flabellare and the banded darter Etheostoma zonale. Etheostoma flabellare has male-only parental care and exhibited slight sexual dimorphism in overall colour, with no discernible effect of season on colour; whereas E. zonale ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12327
更新日期：2014-04-01 00:00:00
abstract：:We assessed the effects of sexual maturity on space use in Atlantic salmon (Salmo salar) parr as facultative early maturation enables us to work on individuals belonging to the same cohort. We monitored the space use of 40 1-year-old males in natura throughout a breeding season. First, mature individuals covered longe...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.14282
更新日期：2020-04-01 00:00:00
abstract：:Ethanol storage and lipid and urea extraction had no effect on bluespotted maskray Neotrygon kuhlii muscle δ13 C values whereas urea-removal and ethanol storage increased δ15 N values. Results presented here show a significant δ15 N increase post-urea removal and provide additional support for this approach in future ...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.13164
更新日期：2017-01-01 00:00:00
abstract：:Accounting for energy use by fishes has been taking place for over 200 years. The original, and continuing gold standard for measuring energy use in terrestrial animals, is to account for the waste heat produced by all reactions of metabolism, a process referred to as direct calorimetry. Direct calorimetry is not easy...
journal_title：Journal of fish biology
pub_type： 历史文章,杂志文章,评审
doi：10.1111/jfb.12824
更新日期：2016-01-01 00:00:00
abstract：:Stable isotope and gut content analyses, in conjunction with backcalculated length-at-age estimates of growth, were employed to examine the relationship between trophic ecology and growth rate of a successful invader, Rutilus rutilus, in eight lakes in Ireland. The data revealed that R. rutilus was a trophic generalis...
journal_title：Journal of fish biology
pub_type： 杂志文章
doi：10.1111/jfb.12351
更新日期：2014-04-01 00:00:00
