Abstract：
:Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48 h (at 20 °C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R2 = 0.93; P = 0.001) and Cd (R2 = 0.98; P = 0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R2 = 0.93; P = 0.0001) and Cd (R2 = 0.91; P = 0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Preez GD,Fourie H,Daneel M,Miller H,Höss S,Ricci C,Engelbrecht G,Zouhar M,Wepener Vdoi
10.1038/s41598-020-61054-7subject
Has Abstractpub_date
2020-03-06 00:00:00pages
4239issue
1issn
2045-2322pii
10.1038/s41598-020-61054-7journal_volume
10pub_type
杂志文章abstract：:A GalNAc/Gal-specific lectin (CGL) from the edible mussel Crenomytilus grayanus has been demonstrated to exhibit antibacterial properties. However, the mechanism of immune modulation by CGL in mammalian cells remains unclear. Here, we demonstrated that CGL can activate immune responses in macrophages and in mice. In t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06647-5
更新日期：2017-07-24 00:00:00
abstract：:Reciprocity is frequently assumed to require complex cognitive abilities. Therefore, it has been argued that reciprocity may be restricted to animals that can meet these demands. Here, we provide evidence for the potential presence of direct reciprocity in teleost fishes. We demonstrate that in pairs of coral reef rab...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14556
更新日期：2015-09-25 00:00:00
abstract：:We provide a proof for the necessity of quantizing fundamental interactions demonstrating that a quantum version is needed for any non trivial conservative interaction whose strength depends on the relative distance between two objects. Our proof is based on a consistency argument that in the presence of a classical f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47844-8
更新日期：2019-08-06 00:00:00
abstract：:We study the capillary retraction of a Newtonian semi-infinite liquid filament through analytical methods. We derive a long-time asymptotic-state expansion for the filament profile using a one-dimensional free-surface slender cylindrical flow model based on the three-dimensional axisymmetric Navier-Stokes equations. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51824-3
更新日期：2019-10-29 00:00:00
abstract：:In this study, we propose a novel sensitive solid-based immunosensor developed on a plasmonic nanopaper platform for the detection of Escherichia coli (E. coli) bacteria. This plasmonic nanopaper that comprises of carboxylated bacterial cellulose (CBC) impregnated with gold nanoparticles (AuNP-CBC), employed as a quen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71285-3
更新日期：2020-09-01 00:00:00
abstract：:Childhood obesity is a risk factor for asthma, but the molecular mechanisms linking both remain elusive. Since obesity leads to chronic low-grade inflammation and affects metabolic signaling we hypothesized that postnatal hyperalimentation (pHA) induced by maternal high-fat-diet during lactation leads to early-onset o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24168
更新日期：2016-04-18 00:00:00
abstract：:Rapid integration of high-quality functional devices in microchannels is in highly demand for miniature lab-on-a-chip applications. This paper demonstrates the embellishment of existing microfluidic devices with integrated micropatterns via femtosecond laser MRAF-based holographic patterning (MHP) microfabrication, wh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33281
更新日期：2016-09-13 00:00:00
abstract：:The North Atlantic is characterized by diatom-dominated spring blooms that results in significant transfer of carbon to higher trophic levels and the deep ocean. These blooms are terminated by limiting silicate concentrations in summer. Numerous regional studies have demonstrated phytoplankton community shifts to ligh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14837-4
更新日期：2017-11-06 00:00:00
abstract：:It is now well established that at a superconductor/ferromagnet (S/F) interface an unconventional superconducting state arises in which the pairing is odd-frequency. The hallmark signature of this superconducting state is generally understood to be an enhancement of the electronic density of states (DoS) at subgap ene...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15483
更新日期：2015-10-27 00:00:00
abstract：:Receding angles have been shown to have great significance when designing a superhydrophobic surface for applications involving self-cleaning. Although apparent receding angles under dynamic conditions have been well studied, the microscopic receding contact line dynamics are not well understood. Therefore, experiment...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08384
更新日期：2015-02-11 00:00:00
abstract：:No consensus currently exist on the optimal treatment of patients with high-risk nonmuscle invasive (HGT1) micropapillary variant of bladder cancer (MPBC). Transcripsome analysis may allow stratification of MPBC-HGT1 enabling prediction of recurrence and guide therapeutic management for individual patients. Whole tran...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76904-7
更新日期：2020-11-18 00:00:00
abstract：:One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32459-2
更新日期：2018-09-20 00:00:00
abstract：:Positron emission tomography (PET) imaging can assist in the early-phase diagnostic and therapeutic evaluation of tumors. Here, we report the radiosynthesis, small animal PET imaging, and biological evaluation of a L-type amino acid transporter 1 (LAT1)-specific PET probe, 18F-FIMP. This probe demonstrates increased t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52270-x
更新日期：2019-10-31 00:00:00
abstract：:Mutations in genes that encode components of tuberous sclerosis complex 2 (TSC2) are associated with tuberous sclerosis complex disease. TSC2 interacts with tuberous sclerosis complex 1 to form a complex that negatively regulates cell growth and proliferation via the inactivation of mechanistic target of rapamycin com...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78274-6
更新日期：2020-12-03 00:00:00
abstract：:In this study, membrane filtration tests showed that the membrane rejection degree of difloxacin hydrochloride (DFHC) increased significantly in the presence of Suwannee River DOM or Aldrich humic acid (2-10 mg-C/L). Titration experiments showed that the excitation and emission of Peak R belonging to DFHC exhibited bl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05635-z
更新日期：2017-07-14 00:00:00
abstract：:The aim of this systematic review was taken to investigate the efficacy and safety of docetaxel plus thalidomide vs. docetaxel alone for treating androgen-independent prostate cancer (AIPC). Data were collected from different databases independently by three researchers according to the pre-defined inclusion and exclu...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep04818
更新日期：2014-04-28 00:00:00
abstract：:We investigated body mass index (BMI) trajectories in the first 2 years of life in 1170 children from an Asian mother-offspring cohort in Singapore, and examined their predictors and associations with childhood cardio-metabolic risk measures at 5 years. Latent class growth mixture modelling analyses were performed to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09046-y
更新日期：2017-08-21 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep30212. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/srep46831
更新日期：2017-05-26 00:00:00
abstract：:Diabetes is a globally prevalent disease that can cause visible microvascular complications such as diabetic retinopathy and macular edema in the human eye retina, the images of which are today used for manual disease screening and diagnosis. This labor-intensive task could greatly benefit from automatic detection usi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47181-w
更新日期：2019-07-24 00:00:00
abstract：:Internet technologies have demonstrated their value for the early detection and prediction of epidemics. In diverse cases, electronic surveillance systems can be created by obtaining and analyzing on-line data, complementing other existing monitoring resources. This paper reports the feasibility of building such a sys...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61686-9
更新日期：2020-03-16 00:00:00
abstract：:Distributed position and orientation systems (DPOSs) can provide abundant time-spatial information for interferometric synthetic aperture radar (InSAR) in airborne earth observation systems. However, some key error terms have not been taken into consideration in the traditional low-order error model, which suppresses ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77595-w
更新日期：2020-11-24 00:00:00
abstract：:We previously developed red lettuce (Lactuca sativa L.) cultivars with high flavonoid and phenolic acid content and demonstrated their anti-diabetic effect. Here we report on developing three fertile and true-breeding lettuce lines enriched with flavonoids with reported beneficial health effects. These lines were iden...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39287-y
更新日期：2019-03-01 00:00:00
abstract：:Autistic spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairment in social communication and restricted/repetitive behavior patterns or interests. Antagonists targeting histamine H3 receptor (H3R) are considered potential therapeutic agents for the therapeutic management of different brain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31385-7
更新日期：2018-08-30 00:00:00
abstract：:N(1)-methyladenosine (m(1)A) is a prominent RNA modification involved in many biological processes. Accurate identification of m(1)A site is invaluable for better understanding the biological functions of m(1)A. However, limitations in experimental methods preclude the progress towards the identification of m(1)A site...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31080
更新日期：2016-08-11 00:00:00
abstract：:Micro-tube experiment has been implemented to understand the mechanisms of governing microcosmic fluid percolation and is extensively used in both fields of micro electromechanical engineering and petroleum engineering. The measured pressure difference across the microtube is not equal to the actual pressure differenc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16870-9
更新日期：2017-11-29 00:00:00
abstract：:Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding reg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29310
更新日期：2016-07-04 00:00:00
abstract：:In clinical diagnostics and pathogen detection, profiling of complex samples for low-level genotypes represents a significant challenge. Advances in speed, sensitivity, and extent of multiplexing of molecular pathogen detection assays are needed to improve patient care. We report the development of an integrated platf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42326
更新日期：2017-02-08 00:00:00
abstract：:The acronymously named "ESKAPE" pathogens represent a group of bacteria that continue to pose a serious threat to human health, not only due to their propensity for repeated emergence, but also due to their ability to "eskape" antibiotic treatment. The evolution of multi-drug resistance in these pathogens alone has gr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41999
更新日期：2017-02-06 00:00:00
abstract：:Tumor progression, limited efficacy of current standard treatments, and the rise in patient mortality are associated with gene expression caused by the synergistic action of intratumoral hypoxia and HIF-1α activation. For this reason, recent investigations have focused on HIF-targeting therapeutic agents, with encoura...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14990-w
更新日期：2017-11-02 00:00:00
abstract：:Adherence to norms and interventions to norm violations are two important forms of social behaviour modelled in economic games. While both appear to serve a prosocial function, they may represent separate mechanisms corresponding with distinct emotional and psychological antecedents, and thus may be predicted by diffe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02952-1
更新日期：2017-06-13 00:00:00
