Intermittent Lactobacilli-containing Vaginal Probiotic or Metronidazole Use to Prevent Bacterial Vaginosis Recurrence: A Pilot Study Incorporating Microscopy and Sequencing.

Abstract:

:Bacterial vaginosis (BV) is associated with HIV acquisition and adverse pregnancy outcomes. Recurrence after metronidazole treatment is high. HIV-negative, non-pregnant Rwandan BV patients were randomized to four groups (n = 17/group) after seven-day oral metronidazole treatment: behavioral counseling only (control), or counseling plus intermittent use of oral metronidazole, Ecologic Femi+ vaginal capsule (containing multiple Lactobacillus and one Bifidobacterium species), or Gynophilus LP vaginal tablet (L. rhamnosus 35) for two months. Vaginal microbiota assessments at all visits included Gram stain Nugent scoring and 16S rRNA gene qPCR and HiSeq sequencing. All interventions were safe. BV (Nugent 7-10) incidence was 10.18 per person-year at risk in the control group, and lower in the metronidazole (1.41/person-year; p = 0.004), Ecologic Femi+ (3.58/person-year; p = 0.043), and Gynophilus LP groups (5.36/person-year; p = 0.220). In mixed effects models adjusted for hormonal contraception/pregnancy, sexual risk-taking, and age, metronidazole and Ecologic Femi+ users, each compared to controls, had higher Lactobacillus and lower BV-anaerobes estimated concentrations and/or relative abundances, and were less likely to have a dysbiotic vaginal microbiota type by sequencing. Inter-individual variability was high and effects disappeared soon after intervention cessation. Lactobacilli-based vaginal probiotics warrant further evaluation because, in contrast to antibiotics, they are not expected to negatively affect gut microbiota or cause antimicrobial resistance.

journal_name

Sci Rep

journal_title

Scientific reports

authors

van de Wijgert JHHM,Verwijs MC,Agaba SK,Bronowski C,Mwambarangwe L,Uwineza M,Lievens E,Nivoliez A,Ravel J,Darby AC

doi

10.1038/s41598-020-60671-6

subject

Has Abstract

pub_date

2020-03-03 00:00:00

pages

3884

issue

1

issn

2045-2322

pii

10.1038/s41598-020-60671-6

journal_volume

10

pub_type

杂志文章
  • Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes.

    abstract::Misfolding and aggregation of prion protein (PrP) causes neurodegenerative diseases like Creutzfeldt-Jakob disease (CJD) and scrapie. Besides the consensus that spontaneous conversion of normal cellular PrPC into misfolded and aggregating PrPSc is the central event in prion disease, an alternative hypothesis suggests ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39213-2

    authors: Czech A,Konarev PV,Goebel I,Svergun DI,Wills PR,Ignatova Z

    更新日期:2019-02-21 00:00:00

  • Divergent mammalian body size in a stable Eocene greenhouse climate.

    abstract::A negative correlation between body size and the latitudinal temperature gradient is well established for extant terrestrial endotherms but less so in the fossil record. Here we analyze the middle Eocene site of Geiseltal (Germany), whose record is considered to span ca. 5 Myrs of gradual global cooling, and generate ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60379-7

    authors: Ring SJ,Bocherens H,Wings O,Rabi M

    更新日期:2020-03-04 00:00:00

  • Folate-Chitosan Nanoparticles Loaded with Ursolic Acid Confer Anti-Breast Cancer Activities in vitro and in vivo.

    abstract::Ursolic acid (UA) has proved to have broad-spectrum anti-tumor effects, but its poor water solubility and incompetent targeting property largely limit its clinical application and efficiency. Here, we synthesized a nanoparticle-based drug carrier composed of chitosan, UA and folate (FA-CS-UA-NPs) and demonstrated that...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30782

    authors: Jin H,Pi J,Yang F,Jiang J,Wang X,Bai H,Shao M,Huang L,Zhu H,Yang P,Li L,Li T,Cai J,Chen ZW

    更新日期:2016-07-29 00:00:00

  • Glider observations of interleaving layers beneath the Kuroshio primary velocity core east of Taiwan and analyses of underlying dynamics.

    abstract::Submesoscale interleaving layers are caused by lateral intrusions of dissimilar water masses in frontal zones, which are significant processes in shaping physical, biogeochemical, and ecological parameters in the ocean. Possible interleaving layers were sometimes observed by ship-based conductivity-temperature-depth (...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47912-z

    authors: Jan S,Wang SH,Yang KC,Yang YJ,Chang MH

    更新日期:2019-08-06 00:00:00

  • Comparison of oral microbiome profiles in stimulated and unstimulated saliva, tongue, and mouth-rinsed water.

    abstract::Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water tha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52445-6

    authors: Jo R,Nishimoto Y,Umezawa K,Yama K,Aita Y,Ichiba Y,Murakami S,Kakizawa Y,Kumagai T,Yamada T,Fukuda S

    更新日期:2019-11-06 00:00:00

  • A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line.

    abstract::Marine bacterial strains are of great interest for their ability to produce secondary metabolites with anticancer potentials. Isolation, identification, characterization and anticancer activities of isolated bacteria from El-Hamra Lake, Wadi El-Natrun (Egypt) were the objectives of this study. The isolated bacteria we...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70945-8

    authors: El-Garawani IM,El-Sabbagh SM,Abbas NH,Ahmed HS,Eissa OA,Abo-Atya DM,Khalifa SAM,El-Seedi HR

    更新日期:2020-08-21 00:00:00

  • A mixed vertebrate eggshell assemblage from the Transylvanian Late Cretaceous.

    abstract::A Late Cretaceous-aged multi-taxon nesting site from Romania preserved in three dimensions reveals the earliest example of nest site sharing yet known from the vertebrate fossil record. Eggshell and osteological evidence combined in this single accumulation demonstrate that at least four vertebrate taxa including enan...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36305-3

    authors: Fernández MS,Wang X,Vremir M,Laurent C,Naish D,Kaiser G,Dyke G

    更新日期:2019-02-13 00:00:00

  • Fine-tuning of lysine side chain modulates the activity of histone lysine methyltransferases.

    abstract::Histone lysine methyltransferases (KMTs) play an important role in epigenetic gene regulation and have emerged as promising targets for drug discovery. However, the scope and limitation of KMT catalysis on substrates possessing substituted lysine side chains remain insufficiently explored. Here, we identify new unnatu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78331-0

    authors: Al Temimi AHK,Merx J,van Noortwijk CJ,Proietti G,Buijs R,White PB,Rutjes FPJT,Boltje TJ,Mecinović J

    更新日期:2020-12-09 00:00:00

  • Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro.

    abstract::Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface. Based on real-tim...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29462

    authors: Yusa K,Yamamoto O,Takano H,Fukuda M,Iino M

    更新日期:2016-07-08 00:00:00

  • The influence of emotional facial expressions on gaze-following in grouped and solitary pedestrians.

    abstract::The mechanisms contributing to collective attention in humans remain unclear. Research indicates that pedestrians utilise the gaze direction of others nearby to acquire environmentally relevant information, but it is not known which, if any, additional social cues influence this transmission. Extending upon previous f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05794

    authors: Gallup AC,Chong A,Kacelnik A,Krebs JR,Couzin ID

    更新日期:2014-07-23 00:00:00

  • A multi-scale model for determining the effects of pathophysiology and metabolic disorders on tumor growth.

    abstract::The search for efficient chemotherapy drugs and other anti-cancer treatments would benefit from a deeper understanding of the tumor microenvironment (TME) and its role in tumor progression. Because in vivo experimental methods are unable to isolate or control individual factors of the TME and in vitro models often do ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59658-0

    authors: Nikmaneshi MR,Firoozabadi B,Mozafari A,Munn LL

    更新日期:2020-02-20 00:00:00

  • Relationship between frequency of hypoglycemic episodes and changes in carotid atherosclerosis in insulin-treated patients with type 2 diabetes mellitus.

    abstract::The effect of hypoglycemia on the progression of atherosclerosis in patients with type 2 diabetes mellitus (T2DM) remains largely unknown. This is a post hoc analysis of a randomized trial to investigate the relationship between hypoglycemic episodes and changes in carotid intima-media thickness (IMT). Among 274 study...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep39965

    authors: Mita T,Katakami N,Shiraiwa T,Yoshii H,Kuribayashi N,Osonoi T,Kaneto H,Kosugi K,Umayahara Y,Gosho M,Shimomura I,Watada H

    更新日期:2017-01-09 00:00:00

  • Resolving new ultrastructural features of cytokinetic abscission with soft-X-ray cryo-tomography.

    abstract::Mammalian cytokinetic abscission is mediated by the ESCRT membrane fission machinery. While much has been clarified on the topology and kinetics of abscission through high-resolution microscopy, key questions regarding the mechanism of abscission remain open. Here we apply cryogenic soft-X-ray tomography to elucidate ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27629

    authors: Sherman S,Kirchenbuechler D,Nachmias D,Tamir A,Werner S,Elbaum M,Elia N

    更新日期:2016-06-10 00:00:00

  • Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase.

    abstract::Telomerase is a ribonucleoprotein enzyme, which maintains genome integrity in eukaryotes and ensures continuous cellular proliferation. Telomerase holoenzyme from the thermotolerant yeast Hansenula polymorpha, in addition to the catalytic subunit (TERT) and telomerase RNA (TER), contains accessory proteins Est1 and Es...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68107-x

    authors: Shepelev NM,Mariasina SS,Mantsyzov AB,Malyavko AN,Efimov SV,Petrova OA,Rodina EV,Zvereva MI,Dontsova OA,Polshakov VI

    更新日期:2020-07-06 00:00:00

  • Ranking the invasions of cheaters in structured populations.

    abstract::The identification of the most influential individuals in structured populations is an important research question, with many applications across the social and natural sciences. Here, we study this problem in evolutionary populations on static networks, where invading cheaters can lead to the collapse of cooperation....

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59020-4

    authors: Yang G,Cavaliere M,Zhu C,Perc M

    更新日期:2020-02-10 00:00:00

  • Exploration of a New Source of Sustainable Nanomaterial from the Koh-e-Suleiman Mountain Range of Pakistan for Industrial Applications.

    abstract::The present study aimed to explore a new source of montmorillonite and to develop an extraction and purification protocol for its isolation from raw clay samples acquired from the Koh-e-Suleiman mountain range in Pakistan. The process involved the collection of raw clay from the source, identification and quantificati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-57511-y

    authors: Nirwan JS,Farhaj S,Chaudhary MM,Khizer Z,Hasan SS,Angelis-Dimakis A,Gill A,Rasheed H,Abbas N,Arshad MS,Hussain T,Shahzad Y,Yousaf AM,Chohan TA,Hussain T,Merchant HA,Akram MR,Khan TM,Ashraf M,Conway BR,Ghori MU

    更新日期:2020-01-17 00:00:00

  • Superdirective dimers of coupled self-resonant split ring resonators: Analytical modelling and numerical and experimental validation.

    abstract::Superdirective antennas developed over the last century have received renewed interest in recent years from the development of metamaterials. These arrays of electromagnetic resonators (or meta-atoms) carrying short wavelength electro- and/or magneto-inductive waves support current distributions with very high spatial...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56988-6

    authors: Vallecchi A,Radkovskaya A,Li L,Faulkner G,Stevens CJ,Shamonina E

    更新日期:2020-01-14 00:00:00

  • Functional Roles of Metabolic Intermediates in Regulating the Human Mitochondrial NAD(P)+-Dependent Malic Enzyme.

    abstract::Human mitochondrial NAD(P)+-dependent malic enzyme (m-NAD(P)-ME) has a dimer of dimers quaternary structure with two independent allosteric sites in each monomer. Here, we reveal the different effects of nucleotide ligands on the quaternary structure regulation and functional role of the human m-NAD(P)-ME exosite. In ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45282-0

    authors: Hsieh JY,Shih WT,Kuo YH,Liu GY,Hung HC

    更新日期:2019-06-24 00:00:00

  • Prediction of amyloid β PET positivity using machine learning in patients with suspected cerebral amyloid angiopathy markers.

    abstract::Amyloid-β(Aβ) PET positivity in patients with suspected cerebral amyloid angiopathy (CAA) MRI markers is predictive of a worse cognitive trajectory, and it provides insights into the underlying vascular pathology (CAA vs. hypertensive angiopathy) to facilitate prognostic prediction and appropriate treatment decisions....

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-75664-8

    authors: Jung YH,Lee H,Kim HJ,Na DL,Han HJ,Jang H,Seo SW

    更新日期:2020-11-02 00:00:00

  • Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.

    abstract::In many human cancers, including hepatocellular carcinoma (HCC), high density of infiltrating tumor-associated macrophages (TAM) is associated with poor prognosis. Most TAMs express a M2 phenotype subsequently supporting tumor growth. How tumor cells polarize these TAMs to a pro-tumor M2 phenotype is still poorly unde...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70137-4

    authors: Shiau DJ,Kuo WT,Davuluri GVN,Shieh CC,Tsai PJ,Chen CC,Lin YS,Wu YZ,Hsiao YP,Chang CP

    更新日期:2020-08-12 00:00:00

  • Can enzyme proximity accelerate cascade reactions?

    abstract::The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on the reaction velocit...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37034-3

    authors: Kuzmak A,Carmali S,von Lieres E,Russell AJ,Kondrat S

    更新日期:2019-01-24 00:00:00

  • Long-term surgical outcomes of primary retropupillary iris claw intraocular lens implantation for the treatment of intraocular lens dislocation.

    abstract::We aimed to investigate the efficacy and safety of primary retropupillary iris claw intraocular lens (R-IOL) implantation in patients with complete intraocular lens (IOL) dislocation. In this single-center retrospective case series, we reviewed the medical records of patients who underwent R-IOL implantation surgery w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80292-3

    authors: Choi EY,Lee CH,Kang HG,Han JY,Byeon SH,Kim SS,Koh HJ,Kim M

    更新日期:2021-01-12 00:00:00

  • Spatial Hearing with Incongruent Visual or Auditory Room Cues.

    abstract::In day-to-day life, humans usually perceive the location of sound sources as outside their heads. This externalized auditory spatial perception can be reproduced through headphones by recreating the sound pressure generated by the source at the listener's eardrums. This requires the acoustical features of the recordin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37342

    authors: Gil-Carvajal JC,Cubick J,Santurette S,Dau T

    更新日期:2016-11-17 00:00:00

  • The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice.

    abstract::There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene function. The present stu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23176-x

    authors: Khan M,Jabeen N,Khan T,Hussain HMJ,Ali A,Khan R,Jiang L,Li T,Tao Q,Zhang X,Yin H,Yu C,Jiang X,Shi Q

    更新日期:2018-03-21 00:00:00

  • Cross-talk between airway and gut microbiome links to IgE responses to house dust mites in childhood airway allergies.

    abstract::A connection between airway and gut microbiota related to allergen exposure in childhood allergies was not well addressed. We aimed to identify the microbiota alterations in the airway and gut related to mite-specific IgE responses in young children with airway allergies. This study enrolled 60 children, including 38 ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70528-7

    authors: Chiu CY,Chan YL,Tsai MH,Wang CJ,Chiang MH,Chiu CC,Su SC

    更新日期:2020-08-10 00:00:00

  • Comparison of phenological traits, growth patterns, and seasonal dynamics of non-structural carbohydrate in Mediterranean tree crop species.

    abstract::Despite non-structural carbohydrate (NSC) importance for tree productivity and resilience, little is known about their seasonal regulations and trade-off with growth and reproduction. We characterize the seasonal dynamics of NSC in relation to the aboveground phenology and temporal growth patterns of three deciduous M...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57016-3

    authors: Tixier A,Guzmán-Delgado P,Sperling O,Amico Roxas A,Laca E,Zwieniecki MA

    更新日期:2020-01-15 00:00:00

  • Hedgehog Interacting Protein (Hhip) Regulates Insulin Secretion in Mice Fed High Fat Diets.

    abstract::Hedgehog interacting protein (Hhip) is essential for islet formation and beta-cell proliferation during pancreatic development; abnormally elevated Hhip expression has been linked to human pancreatitis. Here, we investigate the role of Hhip in modulating insulin secretion in adult Hhip mice (Hhip +/- vs. Hhip+/+) fed ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47633-3

    authors: Nchienzia H,Liao MC,Zhao XP,Chang SY,Lo CS,Chenier I,Ingelfinger JR,Chan JSD,Zhang SL

    更新日期:2019-08-01 00:00:00

  • A cytosolic carbonic anhydrase molecular switch occurs in the gills of metamorphic sea lamprey.

    abstract::Carbonic anhydrase plays a key role in CO2 transport, acid-base and ion regulation and metabolic processes in vertebrates. While several carbonic anhydrase isoforms have been identified in numerous vertebrate species, basal lineages such as the cyclostomes have remained largely unexamined. Here we investigate the repe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33954

    authors: Ferreira-Martins D,McCormick SD,Campos A,Lopes-Marques M,Osório H,Coimbra J,Castro LF,Wilson JM

    更新日期:2016-10-05 00:00:00

  • Effect of aspect ratio and surface defects on the photocatalytic activity of ZnO nanorods.

    abstract::ZnO, aside from TiO2, has been considered as a promising material for purification and disinfection of water and air, and remediation of hazardous waste, owing to its high activity, environment-friendly feature and lower cost. However, their poor visible light utilization greatly limited their practical applications. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04596

    authors: Zhang X,Qin J,Xue Y,Yu P,Zhang B,Wang L,Liu R

    更新日期:2014-04-04 00:00:00

  • Understanding 'saturation' of radar signals over forests.

    abstract::There is an urgent need to quantify anthropogenic influence on forest carbon stocks. Using satellite-based radar imagery for such purposes has been challenged by the apparent loss of signal sensitivity to changes in forest aboveground volume (AGV) above a certain 'saturation' point. The causes of saturation are debate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03469-3

    authors: Joshi N,Mitchard ETA,Brolly M,Schumacher J,Fernández-Landa A,Johannsen VK,Marchamalo M,Fensholt R

    更新日期:2017-06-14 00:00:00