Abstract:
:Liposomes have been one of the most exploited drug delivery systems in recent decades. However, their large-scale production with low batch-to-batch differences is a challenge for industry, which ultimately delays the clinical translation of new products. We have investigated the effects of formulation parameters on the colloidal and biopharmaceutical properties of liposomes generated with a thin-film hydration approach and microfluidic procedure. Dexamethasone hemisuccinate was remotely loaded into liposomes using a calcium acetate gradient. The liposomes produced by microfluidic techniques showed a unilamellar structure, while the liposomes produced by thin-film hydration were multilamellar. Under the same remote loading conditions, a higher loading capacity and efficiency were observed for the liposomes obtained by microfluidics, with low batch-to-batch differences. Both formulations released the drug for almost one month with the liposomes prepared by microfluidics showing a slightly higher drug release in the first two days. This behavior was ascribed to the different structure of the two liposome formulations. In vitro studies showed that both formulations are non-toxic, associate to human Adult Retinal Pigment Epithelial cell line-19 (ARPE-19) cells, and efficiently reduce inflammation, with the liposomes obtained by the microfluidic technique slightly outperforming. The results demonstrated that the microfluidic technique offers advantages to generate liposomal formulations for drug-controlled release with an enhanced biopharmaceutical profile and with scalability.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Al-Amin MD,Bellato F,Mastrotto F,Garofalo M,Malfanti A,Salmaso S,Caliceti Pdoi
10.3390/ijms21051611subject
Has Abstractpub_date
2020-02-26 00:00:00issue
5issn
1422-0067pii
ijms21051611journal_volume
21pub_type
杂志文章abstract::Autocrine signaling is defined as the production and secretion of an extracellular mediator by a cell followed by the binding of that mediator to receptors on the same cell to initiate signaling. Autocrine stimulation often operates in autocrine loops, a type of interaction, in which a cell produces a mediator, for wh...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020977
更新日期:2021-01-19 00:00:00
abstract::Osteosarcoma therapy might be moving toward nanotechnology-based drug delivery systems to reduce the cytotoxicity of antineoplastic drugs and improve their pharmacokinetics. In this paper, we present, for the first time, an extensive chemical and in vitro characterization of dual-loaded photo- and chemo-active keratin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19113670
更新日期:2018-11-20 00:00:00
abstract::KRAS oncogenic mutations are widespread in lung cancer and, because direct targeting of KRAS has proven to be challenging, KRAS-driven cancers lack effective therapies. One alternative strategy for developing KRAS targeted therapies is to identify downstream targets involved in promoting important malignant features, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165806
更新日期:2020-08-13 00:00:00
abstract::Radiation-induced organizing pneumonia (RIOP) is an inflammatory lung disease that is occasionally observed after irradiation to the breast. It is a type of secondary organizing pneumonia that is characterized by infiltrates outside the irradiated volume that are sometimes migratory. Corticosteroids work acutely, but ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18020281
更新日期:2017-01-27 00:00:00
abstract::Estrogen receptor-α positive (ERα⁺) breast cancers represent 75% of all invasive breast cancer cases, while de novo or acquired resistance to ER-directed therapy is also on the rise. Numerous factors contribute to this phenomenon including the recently-reported ESR1 gene mutations such as Y537S, which amplifies co-act...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19020579
更新日期:2018-02-15 00:00:00
abstract::Recent studies have demonstrated that strigolactones (SLs) also participate in the regulation of stress adaptation; however, the regulatory mechanism remains elusive. In this study, the homolog of More Axillary Branches 2, which encodes a key component in SL signaling, in the perennial oil plant Sapium sebiferum was i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20040837
更新日期:2019-02-15 00:00:00
abstract::O-mannosylation is implicated in protein quality control in Saccharomyces cerevisiae due to the attachment of mannose to serine and threonine residues of un- or misfolded proteins in the endoplasmic reticulum (ER). This process also designated as unfolded protein O-mannosylation (UPOM) that ends futile folding cycles ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20246220
更新日期:2019-12-10 00:00:00
abstract::Cotton fibres, as single cells arising from the seed coat, can be classified as lint and fuzz according to their final length. Gossypium arboreum is a cultivated diploid cotton species and a potential donor of the A subgenome of the more widely grown tetraploid cottons. In this study, we performed genetic studies on o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051675
更新日期:2020-02-29 00:00:00
abstract::Radiotherapy for brain tumors induces neuronal DNA damage and may lead to neurodegeneration and cognitive deficits. We investigated the mechanisms of radiation-induced neuronal cell death and the role of miR-711 in the regulation of these pathways. We used in vitro and in vivo models of radiation-induced neuronal cell...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21155239
更新日期:2020-07-23 00:00:00
abstract::Zinc finger protein 554 (ZNF554), a member of the Krüppel-associated box domain zinc finger protein subfamily, is predominantly expressed in the brain and placenta in humans. Recently, we unveiled that ZNF554 regulates trophoblast invasion during placentation and its decreased expression leads to the early pathogenesi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165762
更新日期:2020-08-11 00:00:00
abstract::Neurodegenerative proteinopathies are complex diseases that share some pathogenetic processes. One of these is the failure of the proteostasis network (PN), which includes all components involved in the synthesis, folding, and degradation of proteins, thus leading to the aberrant accumulation of toxic protein aggregat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21176405
更新日期:2020-09-03 00:00:00
abstract::Lonicera japonica Thunb. is a widely used medicinal plant and is rich in a variety of active ingredients. Flavonoids are one of the important components in L. japonica and their content is an important indicator for evaluating the quality of this herb. To study the regulation of flavonoid biosynthesis in L. japonica, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20184494
更新日期:2019-09-11 00:00:00
abstract::Tumors often show intra-tumor heterogeneity because of genotypic differences between all the cells that compose it and that derive from it. Recent studies have shown significant aspects of neuroblastoma heterogeneity that may affect the diagnostic-therapeutic strategy. Therefore, we developed a laboratory protocol, ba...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20040893
更新日期:2019-02-19 00:00:00
abstract::Cell adhesion ability is one of the components to establish cell organization and shows a great contribution to human body construction consisting of various types of cells mixture to orchestrate tissue specific function. The cell adhesion molecule 1 (CADM1) is a molecule of cell adhesion with multiple functions and h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21249732
更新日期:2020-12-20 00:00:00
abstract::Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS) of autoimmune etiology that results from an imbalance between CNS-specific T effector cells and peripheral suppressive mechanisms mediated by regulatory cells (RC). In this research, we collected blood samples from 83 relapsing remi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17091398
更新日期:2016-08-25 00:00:00
abstract::One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoform...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21113813
更新日期:2020-05-27 00:00:00
abstract::This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12021306
更新日期:2011-02-22 00:00:00
abstract::Hepatocyte growth factor (HGF) is an endogenously expressed bioactive substance that has a strong anti-apoptotic effect. In this study, we biochemically and histologically characterized the effects of rh-HGF on in vitro human hepatocyte injury and mouse acute liver failure (ALF) models, both of which were induced by a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20081821
更新日期:2019-04-12 00:00:00
abstract::The interaction between the pituitary hormone, adrenocorticotropin (ACTH), and melanocortin-2 receptor (MC2R) orthologs involves the H6 F7 R8 W9 and R/K15 K16 R17 R18 motifs in ACTH making contact with corresponding contact sites on MC2R. Earlier studies have localized the common HFRW binding site of all melanocortin ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174166
更新日期:2019-08-26 00:00:00
abstract::Hypoxia is a common environmental stress factor and is associated with fibrogenesis. Matrix metalloproteinase-2 (MMP-2), produced by hepatic stellate cells (HSCs), plays an important role in liver fibrogenesis. However, inconsistent results have been reported on the impact of hypoxia on MMP-2 expression and activity i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12042434
更新日期:2011-01-01 00:00:00
abstract::Fingertip response to trauma represents a fascinating example of tissue regeneration. Regeneration derives from proliferative mesenchymal cells (blastema) that subsequently differentiate into soft and skeletal tissues. Clinically, conservative treatment of the amputated fingertip under occlusive dressing can shift the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18051054
更新日期:2017-05-13 00:00:00
abstract::Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215474
更新日期:2019-11-03 00:00:00
abstract::Over 70 different genetic variants with a significant association with rheumatoid arthritis (RA) have been discovered. Anti-citrullination protein antibodies (ACPA)-positive RA variants are more well-defined than their ACPA-negative counterparts. The human leukocyte antigen, HLA-DRB1 locus remains the prime suspect in...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17060900
更新日期:2016-06-08 00:00:00
abstract::Increasing photosynthetic ability as a whole is essential for acquiring higher crop yields. Nonleaf green organs (NLGOs) make important contributions to photosynthate formation, especially under stress conditions. However, there is little information on the pod wall in legume forage related to seed development and yie...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124457
更新日期:2020-06-23 00:00:00
abstract::Cucumber green mottle mosaic virus (CGMMV) is a member of the genus Tobamovirus, which cause diseases in cucurbits, especially watermelon. In watermelon, symptoms develop on the whole plant, including leaves, stems, peduncles, and fruit. To better understand the molecular mechanisms of watermelon early responses to CG...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20030610
更新日期:2019-01-31 00:00:00
abstract::Pulmonary fibrosis is a chronic and progressive lung disease characterized by the activation of fibroblasts and the irreversible deposition of connective tissue matrices that leads to altered pulmonary architecture and physiology. Multiple factors have been implicated in the pathogenesis of lung fibrosis, including ge...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21113878
更新日期:2020-05-29 00:00:00
abstract::Atherosclerosis, as a chronic inflammatory disease, is the major underlying cause of death worldwide. However, the mechanisms that underlie the inflammatory process are not completely understood. Prolyl-isomerase-1 (Pin1), as a unique peptidyl-prolyl isomerase, plays an important role in inflammation and endothelial d...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030644
更新日期:2017-03-16 00:00:00
abstract::Chondrocyte dysfunction occurs during the development of osteoarthritis (OA), typically resulting from a deleterious increase in oxidative stress. Accordingly, strategies for arresting oxidative stress-induced chondrocyte dysfunction may lead to new potential therapeutic targets for OA treatment. Forkhead box O (FoxO)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123794
更新日期:2018-11-28 00:00:00
abstract::Balanced osteoclast and osteoblast activity is necessary for skeletal health, whereas unbalanced osteoclast activity causes bone loss in many skeletal conditions. A better understanding of pathways that regulate osteoclast differentiation and activity is necessary for the development of new therapies to better manage ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21031056
更新日期:2020-02-05 00:00:00
abstract::Aluminum-activated malate transporters (ALMTs) play an important role in aluminum tolerance, stomatal opening, and fruit acidity in plants. However, the evolutionary pattern of the ALMT gene family in apples remains relatively unknown. In this study, a total of 25 MdALMT genes were identified from the apple reference ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19092807
更新日期:2018-09-18 00:00:00