Abstract:
:Understanding how animals respond to injury and how wounds heal remains a challenge. These questions can be addressed using genetically tractable animals, including the nematode Caenorhabditis elegans. Given its small size, the current methods for inflicting wounds in a controlled manner are demanding. To facilitate and accelerate the procedure, we fabricated regular arrays of pyramidal features ("pins") sharp enough to pierce the tough nematode cuticle. The pyramids were made from monocrystalline silicon wafers that were micro-structured using optical lithography and alkaline wet etching. The fabrication protocol and the geometry of the pins, determined by electron microscopy, are described in detail. We also used electron microscopy to characterize the different types of injury caused by these pins. Upon wounding, C. elegans expresses genes encoding antimicrobial peptides. A comparison of the induction of antimicrobial peptide gene expression using traditional needles and the pin arrays demonstrates the utility of this new method.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Belougne J,Ozerov I,Caillard C,Bedu F,Ewbank JJdoi
10.1038/s41598-020-60333-7subject
Has Abstractpub_date
2020-02-27 00:00:00pages
3581issue
1issn
2045-2322pii
10.1038/s41598-020-60333-7journal_volume
10pub_type
杂志文章abstract::Compact silicon integrated lasers are of significant interest for various applications. We present a detailed investigation for realizing sub-mm long on-chip laser structures operating at λ = 1.533 µm on the silicon-on-insulator photonic platform by combining a multi-segment silicon waveguide structure and a recently ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67722-y
更新日期:2020-07-02 00:00:00
abstract::Air pollution has aroused significant public concern in China, therefore, long-term air-quality data with high temporal and spatial resolution are needed to understand the variations of air pollution in China. However, the yearly variations with high spatial resolution of air quality and six air pollutants are still u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50655-6
更新日期:2019-10-23 00:00:00
abstract::Rabi splitting and Fano resonance are well-known physical phenomena in conventional quantum systems as atoms and quantum dots, arising from strong interaction between two quantum states. In recent years similar features have been observed in various nanophotonic and nanoplasmonic systems. Yet, realization of strong in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08221-5
更新日期:2017-08-14 00:00:00
abstract::To master linguistic communication, humans must acquire large vocabularies quickly and effortlessly. Efficient word learning might be facilitated by the ability to rapidly acquire novel word forms even outside the focus of attention, occurring within minutes of repetitive exposure and suggesting fast and automatic lex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34029-y
更新日期:2018-10-25 00:00:00
abstract::Highly coordinated and coincidental patterns of activity-dependent mechanisms ("fire together wire together") are thought to serve as inductive signals during synaptogenesis, enabling neuronal pairing between specific sub-sets of excitatory partners. However, neither the nature of activity triggers, nor the "activity ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09523
更新日期:2015-04-01 00:00:00
abstract::Human corneal endothelial cells (HCECs) easily become fibroblastic-like when cultured, rendering them unsuitable for tissue engineering of the cornea. Transforming growth factor β (TGF-β) could be a key factor in this phenomenon; however, TGF-β is also known to maintain the endothelium in a quiescent state in vivo. Th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22821-9
更新日期:2018-03-13 00:00:00
abstract::Different grazing strategies impact grassland plant production and may also regulate the soil carbon formation. For a site in semiarid temperate steppe, we studied the effect of combinations of rest, high and moderate grazing pressure over three stages of the growing season, on the process involved in soil carbon sequ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10892
更新日期:2015-07-03 00:00:00
abstract::The development of physiologically active semiochemicals is largely limited by the labor-consuming searching process. How to screen active semiochemicals efficiently is of significance to the extension of behavior regulation in pest control. Here pharmacophore modeling and shape-based virtual screening were combined t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34600
更新日期:2016-10-06 00:00:00
abstract::Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10627-0
更新日期:2017-09-04 00:00:00
abstract::Phthalates are commonly included as ingredients in personal care products such as cosmetics, shampoos and perfumes. Diethyl phthalate (DEP) has been found to be anti-androgenic and linked with adverse reproductive effects on males, but effects on females are poorly understood. We designed an integrative and translatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63904-w
更新日期:2020-04-27 00:00:00
abstract::Colorectal cancer (CRC) is a cancer of growing incidence that associates with a high mortality rate worldwide. There is a poor understanding of the heterogeneity of CRC with regard to causative genetic mutations and gene regulatory mechanisms. Previous studies have identified several susceptibility genes in small-scal...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30624
更新日期:2016-08-01 00:00:00
abstract::The 8th edition of TNM staging system has been released and it incorporates many changes to the T and N classifications for pancreatic cancer. Comparative study between the 7th and 8th edition of TNM staging system from Asian population has not been reported yet. This study aimed to compare the 7th and 8th edition of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28193-4
更新日期:2018-07-10 00:00:00
abstract::Synchrotron X-ray imaging technique has been used to investigate biofluid flows in a non-destructive manner. This study aims to investigate the feasibility of the X-ray PIV technique with CO2 microbubbles as flow tracer for measurement of pulsatile blood flows under in vivo conditions. The traceability of CO2 microbub...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08840
更新日期:2015-03-06 00:00:00
abstract::Metabolomic and transcriptomic approaches were used to dissect the enhanced disease resistance in the plants harbouring a RNA interfering construct of OsWRKY62 and OsWRKY76 (dsOW62/76) genes. The primary metabolic pathways were activated in dsOW62/76 compared with wild-type (ZH17) plants, revealed by increased accumul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02643-x
更新日期:2017-05-30 00:00:00
abstract::At present, there is a growing interest in the study of the neurotropic activity of polyunsaturated fatty acids ethanolamides (N-acylethanolamines). N-docosahexaenoylethanolamine (DHEA, synaptamide) is an endogenous metabolite and structural analogue of anandamide, a widely studied endocannabinoid derived from arachid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80818-9
更新日期:2021-01-12 00:00:00
abstract::Liquid biopsy can provide a strong basis for precision medicine. We aimed to identify novel single-nucleotide variants (SNVs) in circulating tumor DNA (ctDNA) in patients with hepatocellular carcinoma (HCC). Deep sequencing of plasma-derived ctDNA from 59 patients with HCC was performed using a panel of 2924 SNVs in 6...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74494-y
更新日期:2020-10-20 00:00:00
abstract::Intrahepatic cholestasis induced by drug toxicity, bile salt export pump (BSEP) deficiency, or pregnancy frequently causes cholestatic liver damage, which ultimately may lead to liver fibrosis and cirrhosis. Here, the preventive and therapeutic effects of peroxisome proliferator-activated receptor α (PPARα) signaling ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10524-6
更新日期:2017-08-30 00:00:00
abstract::Understanding the phylogeography and genetic structure of populations and the processes responsible of patterns therein is crucial for evaluating the vulnerability of marine species and developing management strategies. In this study, we explore how past climatic events and ongoing oceanographic and demographic proces...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33269
更新日期:2016-09-15 00:00:00
abstract::Temporal-difference (TD) learning models afford the neuroscientist a theory-driven roadmap in the quest for the neural mechanisms of reinforcement learning. The application of these models to understanding the role of phasic midbrain dopaminergic responses in reward prediction learning constitutes one of the greatest ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42244-4
更新日期:2019-04-12 00:00:00
abstract::Direct estimation of Lagrangian turbulence statistics is essential for the proper modeling of dispersion and transport in highly obstructed canopy flows. However, Lagrangian flow measurements demand very high rates of data acquisition, resulting in bottlenecks that prevented the estimation of Lagrangian statistics in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43555-2
更新日期:2019-05-15 00:00:00
abstract::A better understanding of the mechanisms underlying cell tropisms and the efficiency of viral infection is critical for the development of vaccines and antiviral drugs for viral diseases. In this study, we worked on the entry mechanisms of guinea pig cytomegalovirus and found that endogenous expression of a combinatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65545-5
更新日期:2020-05-22 00:00:00
abstract::In species richness studies, citizen-science surveys where participants make individual decisions regarding sampling strategies provide a cost-effective approach to collect a large amount of data. However, it is unclear to what extent the bias inherent to opportunistically collected samples may invalidate our inferenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55593-x
更新日期:2019-12-13 00:00:00
abstract::Poor oral health is not only associated with diabetes and cardiovascular disease but adverse pregnancy outcomes. However the influence of dental caries on pregnancy is unknown. The aim of this study was to evaluate the association between dental caries and adverse pregnancy outcomes and the effect of treatment for den...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62306-2
更新日期:2020-03-24 00:00:00
abstract::Brain trauma was clinically associated with increased osteogenesis in the appendicular skeleton. We showed previously in C57BL/6J mice that mild traumatic brain injury (mTBI) transiently induced bone formation in the femur via the cannabinoid-1 (CB1) receptor. Here, we subjected ICR mice to mTBI and examined the bone ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51720-w
更新日期:2019-11-07 00:00:00
abstract::If the components of a battery, including electrodes, separator, electrolyte and the current collectors can be designed as paints and applied sequentially to build a complete battery, on any arbitrary surface, it would have significant impact on the design, implementation and integration of energy storage devices. Her...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00481
更新日期:2012-01-01 00:00:00
abstract::Both attraction and repulsion from linearly polarized light have been observed in zooplankton. A dichotomous choice experiment, consisting of plankton light traps deployed in natural waters at a depth of 30 m that projected either polarized or unpolarized light of the same intensity, was used to test the hypothesis th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35891
更新日期:2016-10-20 00:00:00
abstract::An in vitro-transcribed RNA aptamer (trans-RA16) that targets non-small cell lung cancer (NSCLC) was previously identified through in vivo SELEX. Trans-RA16 can specifically target and inhibit human NCI-H460 cells in vitro and xenograft tumors in vivo. Here, in a follow-up study, we obtained a chemically-synthesized v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55280-x
更新日期:2019-12-11 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-23087-x
更新日期:2018-03-19 00:00:00
abstract::We investigated the ocular surface (OS) system modifications after completely successful glaucoma surgery in thirty-eight patients undergoing trabeculectomy (surgical group), using laser scanning confocal microscopy (LSCM) and impression cytology (IC). Twenty-six medically controlled glaucomatous patients served as co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47823-z
更新日期:2019-08-05 00:00:00
abstract::Species in the genus Suaeda have few diagnostic characters and substantial morphological plasticity. Hence, regional floras do not provide clear taxonomic information for Suaeda spp. in East Asia. In order to assess the taxonomy of four species in the genus Suaeda (S. australis, S. maritima, S. japonica, and S. hetero...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78041-7
更新日期:2020-12-01 00:00:00