Abstract：
:The homeostatic control of lipid metabolism is essential for many fundamental physiological processes. A deep understanding of its regulatory mechanisms is pivotal to unravel prospective physiopathological factors and to identify novel molecular targets that could be employed to design promising therapies in the management of lipid disorders. Here, we investigated the role of bromodomain and extraterminal domain (BET) proteins in the regulation of lipid metabolism. To reach this aim, we used a loss-of-function approach by treating HepG2 cells with JQ1, a powerful and selective BET inhibitor. The main results demonstrated that BET inhibition by JQ1 efficiently decreases intracellular lipid content, determining a significant modulation of proteins involved in lipid biosynthesis, uptake and intracellular trafficking. Importantly, the capability of BET inhibition to slow down cell proliferation is dependent on the modulation of cholesterol metabolism. Taken together, these data highlight a novel epigenetic mechanism involved in the regulation of lipid homeostasis.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tonini C,Colardo M,Colella B,Di Bartolomeo S,Berardinelli F,Caretti G,Pallottini V,Segatto Mdoi
10.3390/ijms21041297subject
Has Abstractpub_date
2020-02-14 00:00:00issue
4issn
1422-0067pii
ijms21041297journal_volume
21pub_type
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