Intrathecal Injection in A Rat Model: A Potential Route to Deliver Human Wharton's Jelly-Derived Mesenchymal Stem Cells into the Brain.


:Mesenchymal stem cells (MSCs) are considered as promising therapeutic agents for neurodegenerative disorders because they can reduce underlying pathology and also repair damaged tissues. Regarding the delivery of MSCs into the brain, intravenous and intra-arterial routes may be less feasible than intraparenchymal and intracerebroventricular routes due to the blood-brain barrier. Compared to the intraparenchymal or intracerebroventricular routes, however, the intrathecal route may have advantages: this route can deliver MSCs throughout the entire neuraxis and it is less invasive since brain surgery is not required. The objective of this study was to investigate the distribution of human Wharton's jelly-derived MSCs (WJ-MSCs) injected via the intrathecal route in a rat model. WJ-MSCs (1 × 106) were intrathecally injected via the L2-3 intervertebral space in 6-week-old Sprague Dawley rats. These rats were then sacrificed at varying time points: 0, 6, and 12 h following injection. At 12 h, a significant number of MSCs were detected in the brain but not in other organs. Furthermore, with a 10-fold higher dose of WJ-MSCs, there was a substantial increase in the number of cells migrating to the brain. These results suggest that the intrathecal route can be a promising route for the performance of stem cell therapy for CNS diseases.


Int J Mol Sci


Kim H,Na DL,Lee NK,Kim AR,Lee S,Jang H




Has Abstract


2020-02-13 00:00:00










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    abstract::Glycosyltransferases that use polyisoprenol-linked donor substrates are categorized in the GT-C superfamily. In eukaryotes, they act in the endoplasmic reticulum (ER) lumen and are involved in N-glycosylation, glypiation, O-mannosylation, and C-mannosylation of proteins. We generated a membrane topology model of C-man...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Albuquerque-Wendt A,Hütte HJ,Buettner FFR,Routier FH,Bakker H

    更新日期:2019-09-29 00:00:00

  • Human Leukocyte Antigen Class II Alleles Are Associated with Hepatitis C Virus Natural Susceptibility in the Chinese Population.

    abstract::Human leukocyte antigen (HLA) class II molecule influences host antigen presentation and anti-viral immune response. The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) within HLA class II gene were associated with different clinical outcomes of hepatitis C virus (HCV) infection. Th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Yue M,Xu K,Wu MP,Han YP,Huang P,Peng ZH,Wang J,Su J,Yu RB,Li J,Zhang Y

    更新日期:2015-07-23 00:00:00

  • Principal component analysis of HPLC retention data and molecular modeling structural parameters of cardiovascular system drugs in view of their pharmacological activity.

    abstract::Evaluation of relationships between molecular modeling structural parameters and high-performance liquid chromatography (HPLC) retention data of 11 cardiovascular system drugs by principal component analysis (PCA) in relation to their pharmacological activity was performed. The six retention data parameters were deter...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Stasiak J,Koba M,Bober L,Baczek T

    更新日期:2010-07-09 00:00:00

  • Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells.

    abstract::Milk fat is a key factor affecting milk quality and is also a major trait targeted in dairy cow breeding. To determine how the synthesis and the metabolism of lipids in bovine milk is regulated at the miRNA level, primary mammary epithelial cells (pMEC) derived from two Chinese Holstein dairy cows that produced extrem...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Shen B,Zhang L,Lian C,Lu C,Zhang Y,Pan Q,Yang R,Zhao Z

    更新日期:2016-02-17 00:00:00

  • Transcriptome Analysis Identifies Candidate Genes and Functional Pathways Controlling the Response of Two Contrasting Barley Varieties to Powdery Mildew Infection.

    abstract::Powdery mildew caused by Blumeria graminis f. sp. hordei (Bgh) is one of the most serious diseases in barley. The numerous barley varieties across China provide valuable genetic resources to screen the resistant germplasm and to discover the primary genes of resistance to powdery mildew. In this study, Chinese barley ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li Y,Guo G,Zhou L,Chen Y,Zong Y,Huang J,Lu R,Liu C

    更新日期:2019-12-24 00:00:00

  • Applications of CRISPR/Cas System to Bacterial Metabolic Engineering.

    abstract::The clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR/Cas) adaptive immune system has been extensively used for gene editing, including gene deletion, insertion, and replacement in bacterial and eukaryotic cells owing to its simple, rapid, and efficient activities in unprecedented res...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Cho S,Shin J,Cho BK

    更新日期:2018-04-05 00:00:00

  • Urinary Metabolic Phenotyping Reveals Differences in the Metabolic Status of Healthy and Inflammatory Bowel Disease (IBD) Children in Relation to Growth and Disease Activity.

    abstract:BACKGROUND:Growth failure and delayed puberty are well known features of children and adolescents with inflammatory bowel disease (IBD), in addition to the chronic course of the disease. Urinary metabonomics was applied in order to better understand metabolic changes between healthy and IBD children. METHODS:21 Pediat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Martin FP,Ezri J,Cominetti O,Da Silva L,Kussmann M,Godin JP,Nydegger A

    更新日期:2016-08-11 00:00:00

  • The Emerging Role of Long Non-Coding RNAs in Plant Defense Against Fungal Stress.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang H,Guo H,Hu W,Ji W

    更新日期:2020-04-11 00:00:00

  • Polyunsaturated Fatty Acids: What is Their Role in Treatment of Psychiatric Disorders?

    abstract::In the central nervous system omega-3 fatty acids modulate cell signaling and affect dopaminergic and serotonergic pathways. On this basis, a new application for omega-3 fatty acids has been proposed, concerning the treatment of several psychiatric disorders. The present article is an update of a previous systematic r...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Bozzatello P,Rocca P,Mantelli E,Bellino S

    更新日期:2019-10-23 00:00:00

  • The Impact of Carnitine on Dietary Fiber and Gut Bacteria Metabolism and Their Mutual Interaction in Monogastrics.

    abstract::Carnitine has vital roles in the endogenous metabolism of short chain fatty acids. It can protect and support gut microbial species, and some dietary fibers can reduce the available iron involved in the bioactivity of carnitine. There is also an antagonistic relationship between high microbial populations and carnitin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Ghonimy A,Zhang DM,Farouk MH,Wang Q

    更新日期:2018-03-28 00:00:00

  • Targeted Therapy in Melanoma and Mechanisms of Resistance.

    abstract::The common mutation BRAFV600 in primary melanomas activates the mitogen-activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) pathway and the introduction of proto-oncogene B-Raf (BRAF) and mitogen-activated protein kinase kinase (MEK) inhibitors (BRAFi and MEKi) was a breakthrough in the treatment...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Czarnecka AM,Bartnik E,Fiedorowicz M,Rutkowski P

    更新日期:2020-06-27 00:00:00

  • Luminescence of Cypridina Luciferin in the Presence of Human Plasma Alpha 1-Acid Glycoprotein.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kanie S,Komatsu M,Mitani Y

    更新日期:2020-10-12 00:00:00

  • Anti-Obesity Effects of Dietary Calcium: The Evidence and Possible Mechanisms.

    abstract::Obesity is a serious health challenge worldwide and is associated with various comorbidities, including dyslipidemia, type 2 diabetes, and cardiovascular disease. Developing effective strategies to prevent obesity is therefore of paramount importance. One potential strategy to reduce obesity is to consume calcium, whi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang F,Ye J,Zhu X,Wang L,Gao P,Shu G,Jiang Q,Wang S

    更新日期:2019-06-23 00:00:00

  • p-Cresol Sulfate Caused Behavior Disorders and Neurodegeneration in Mice with Unilateral Nephrectomy Involving Oxidative Stress and Neuroinflammation.

    abstract::Protein-bound uremic toxins, such as p-cresol sulfate (PCS), can be accumulated with declined renal function and aging and is closely linked with central nervous system (CNS) diseases. In the periphery, PCS has effects on oxidative stress and inflammation. Since oxidative stress and inflammation have substantial roles...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sun CY,Li JR,Wang YY,Lin SY,Ou YC,Lin CJ,Wang JD,Liao SL,Chen CJ

    更新日期:2020-09-12 00:00:00

  • Kynurenine Aminotransferase Isozyme Inhibitors: A Review.

    abstract::Kynurenine aminotransferase isozymes (KATs 1-4) are members of the pyridoxal-5'-phosphate (PLP)-dependent enzyme family, which catalyse the permanent conversion of l-kynurenine (l-KYN) to kynurenic acid (KYNA), a known neuroactive agent. As KATs are found in the mammalian brain and have key roles in the kynurenine pat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Nematollahi A,Sun G,Jayawickrama GS,Church WB

    更新日期:2016-06-15 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ernest HB,Drazenovich TL,Dalbeck LS,Hawkins MG

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  • Role of Inflammation in Diabetic Retinopathy.

    abstract::Diabetic retinopathy is a common complication of diabetes and remains the leading cause of blindness among the working-age population. For decades, diabetic retinopathy was considered only a microvascular complication, but the retinal microvasculature is intimately associated with and governed by neurons and glia, whi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Rübsam A,Parikh S,Fort PE

    更新日期:2018-03-22 00:00:00

  • First Identification of RNA-Binding Proteins That Regulate Alternative Exons in the Dystrophin Gene.

    abstract::The Duchenne muscular dystrophy (DMD) gene has a complex expression pattern regulated by multiple tissue-specific promoters and by alternative splicing (AS) of the resulting transcripts. Here, we used an RNAi-based approach coupled with DMD-targeted RNA-seq to identify RNA-binding proteins (RBPs) that regulate splicin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Miro J,Bougé AL,Murauer E,Beyne E,Da Cunha D,Claustres M,Koenig M,Tuffery-Giraud S

    更新日期:2020-10-21 00:00:00

  • Overexpression of ThMYC4E Enhances Anthocyanin Biosynthesis in Common Wheat.

    abstract::The basic helix-loop helix (bHLH) transcription factor has been inferred to play an important role in blue and purple grain traits in common wheat, but to date, its overexpression has not been reported. In this study, the bHLH transcription factor ThMYC4E, the candidate gene controlling the blue grain trait from Th. P...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhao S,Xi X,Zong Y,Li S,Li Y,Cao D,Liu B

    更新日期:2019-12-24 00:00:00

  • The Study of Zinc Ions Binding to αS1-, β- and κ-Casein.

    abstract::The presented studies focused on the specificity binding of particular casein fractions: αS1-, β- and κ-casein (αS1CN, βCN, κCN), with zinc ions. The binding mechanism was determined by kinetic modeling using results of batch sorption. For this goal, models of zero-order kinetics, pseudo-first-order, pseudo-second-ord...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Rodzik A,Pomastowski P,Railean-Plugaru V,Sprynskyy M,Buszewski B

    更新日期:2020-10-30 00:00:00

  • Platelet-Released Growth Factors and Platelet-Rich Fibrin Induce Expression of Factors Involved in Extracellular Matrix Organization in Human Keratinocytes

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Bayer A,Wijaya B,Möbus L,Rademacher F,Rodewald M,Tohidnezhad M,Pufe T,Drücke D,Gläser R,Harder J

    更新日期:2020-06-20 00:00:00

  • Constitutive Differential Features of Type 2 Transglutaminase in Cells Derived from Celiac Patients and from Healthy Subjects.

    abstract::Type 2 transglutaminase (TG2) is a ubiquitous enzyme able to modify gliadin peptides introduced into the organism through the diet. By means of its catalytic activity, TG2 seems to have an important pathogenetic role in celiac disease (CD), an inflammatory intestinal disease caused by the ingestion of gluten-containin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Paolella G,Nanayakkara M,Sposito S,Lepretti M,Auricchio S,Esposito C,Barone MV,Martucciello S,Caputo I

    更新日期:2020-02-12 00:00:00

  • Diversity and biosynthetic potential of culturable actinomycetes associated with marine sponges in the China Seas.

    abstract::The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Xi L,Ruan J,Huang Y

    更新日期:2012-01-01 00:00:00

  • The Role of Tumor Microenvironment in Chemoresistance: To Survive, Keep Your Enemies Closer.

    abstract::Chemoresistance is a leading cause of morbidity and mortality in cancer and it continues to be a challenge in cancer treatment. Chemoresistance is influenced by genetic and epigenetic alterations which affect drug uptake, metabolism and export of drugs at the cellular levels. While most research has focused on tumor c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Senthebane DA,Rowe A,Thomford NE,Shipanga H,Munro D,Mazeedi MAMA,Almazyadi HAM,Kallmeyer K,Dandara C,Pepper MS,Parker MI,Dzobo K

    更新日期:2017-07-21 00:00:00

  • Stem cell-based neuroprotective and neurorestorative strategies.

    abstract::Stem cells, a special subset of cells derived from embryo or adult tissues, are known to present the characteristics of self-renewal, multiple lineages of differentiation, high plastic capability, and long-term maintenance. Recent reports have further suggested that neural stem cells (NSCs) derived from the adult hipp...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Hung CW,Liou YJ,Lu SW,Tseng LM,Kao CL,Chen SJ,Chiou SH,Chang CJ

    更新日期:2010-05-05 00:00:00

  • Thymus Inception: Molecular Network in the Early Stages of Thymus Organogenesis.

    abstract::The thymus generates central immune tolerance by producing self-restricted and self-tolerant T-cells as a result of interactions between the developing thymocytes and the stromal microenvironment, mainly formed by the thymic epithelial cells. The thymic epithelium derives from the endoderm of the pharyngeal pouches, e...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Figueiredo M,Zilhão R,Neves H

    更新日期:2020-08-11 00:00:00

  • Bioactive Phytochemicals from Wild Arbutus unedo L. Berries from Different Locations in Portugal: Quantification of Lipophilic Components.

    abstract::The lipophilic composition of wild Arbutus unedo L. berries, collected from six locations in Penacova (center of Portugal), as well as some general chemical parameters, namely total soluble solids, pH, titratable acidity, total phenolic content and antioxidant activity was studied in detail to better understand its po...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Fonseca DF,Salvador ÂC,Santos SA,Vilela C,Freire CS,Silvestre AJ,Rocha SM

    更新日期:2015-06-23 00:00:00

  • MYC-Associated Factor MAX is a Regulator of the Circadian Clock.

    abstract::The circadian transcriptional network is based on a competition between transcriptional activator and repressor complexes regulating the rhythmic expression of clock-controlled genes. We show here that the MYC-associated factor X, MAX, plays a repressive role in this network and operates through a MYC-independent bind...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Blaževitš O,Bolshette N,Vecchio D,Guijarro A,Croci O,Campaner S,Grimaldi B

    更新日期:2020-03-26 00:00:00

  • Inter-Species Differences in Regulation of the Progranulin-Sortilin Axis in TDP-43 Cell Models of Neurodegeneration.

    abstract::Cytoplasmic aggregates and nuclear depletion of the ubiquitous RNA-binding protein TDP-43 have been described in the autoptic brain tissues of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTLD) patients and both TDP-43 loss-of-function and gain-of-function mechanisms seem to contribute to the neuro...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gumina V,Onesto E,Colombrita C,Maraschi A,Silani V,Ratti A

    更新日期:2019-11-22 00:00:00

  • Nanosecond T-jump experiment in poly(glutamic acid): a circular dichroism study.

    abstract::Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure p...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mendonça L,Hache F

    更新日期:2012-01-01 00:00:00