N,N-dimethyltryptamine reduces infarct size and improves functional recovery following transient focal brain ischemia in rats.


BACKGROUND AND PURPOSE:N,N-dimethyltryptamine (DMT) is an endogenous ligand of the Sigma 1 receptor (Sig-1R) with documented in vitro cytoprotective properties against hypoxia. Our aim was to demonstrate the in vivo neuroprotective effect of DMT following ischemia-reperfusion injury in the rat brain. METHODS:Transient middle cerebral occlusion (MCAO) was induced for 60 min in male Wistar rats using the filament occlusion model under general anaesthesia. Before the removal of the filament the treatment group (n = 10) received an intra-peritoneal (IP) bolus of 1 mg/kg-body weight (bw) DMT dissolved in 1 ml 7% ethanol/saline vehicle, followed by a maintenance dose of 2 mg/Kg-bw/h delivered over 24 h via osmotic minipumps. Controls (n = 10) received a vehicle bolus only. A third group (n = 10) received a Sig-1R antagonist (BD1063, 1 mg/kg-bw bolus +2 mg/kg-bw/h maintenance) in parallel with the DMT. Lesion volume was measured by MRI 24 h following the MCAO. Shortly after imaging the animals were terminated, and the native brains and sera were removed. Four rats were perfusion fixed. Functional recovery was studied in two separate group of pre-trained animals (n = 8-8) using the staircase method for 30 days. The expression levels of proteins involved in apoptosis, neuroplasticity and inflammatory regulation were assessed by real-time qPCR and ELISA. RESULTS:DMT treated rats were characterized by lower ischemic lesion volume (p = .0373), and better functional recovery (p = .0084) compared to the controls. Sig-1R was expressed both in neurons and in microglia in the peri-infarct cortex, and the DMT induced change in the lesion volume was hindered by BD1063. Lower APAF1 expression (mRNA and protein) and higher BNDF levels were documented on DTM, while decreased TNF-α, IL1-β, IL-6 and increased IL-10 expressions indicated the compound's anti-inflammatory potential. CONCLUSION:Our results indicate a Sig-1R dependent reduction of the ischemic brain injury following exogenous DMT administration in rats, presumably through a combined anti-apoptotic, pro-neurotrophic and anti-inflammatory treatment effect.


Exp Neurol


Experimental neurology


Nardai S,László M,Szabó A,Alpár A,Hanics J,Zahola P,Merkely B,Frecska E,Nagy Z




Has Abstract


2020-05-01 00:00:00












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  • Chronic phenytoin administration alters the metabolic profile of superficial gastrocnemius muscle fibers in dystrophic mice.

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    authors: Younger LE,Silverman H

    更新日期:1984-04-01 00:00:00

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    journal_title:Experimental neurology

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    authors: Rusovan A,Kanje M

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  • Ghrelin prevents 1-methyl-4-phenylpyridinium ion-induced cytotoxicity through antioxidation and NF-kappaB modulation in MES23.5 cells.

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    authors: Liu L,Xu H,Jiang H,Wang J,Song N,Xie J

    更新日期:2010-03-01 00:00:00

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    authors: Chung RS,McCormack GH,King AE,West AK,Vickers JC

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  • Use of cell-based assays in myasthenia gravis and other antibody-mediated diseases.

    abstract::The increasing demand on diagnostic assays that are sensitive and specific for pathogenic antibodies, and the interest in identifying new antigens, prompted the development of cell-based assays for the detection of autoantibodies in myasthenia gravis and other autoimmune disorders. Cell-based assays were initially use...

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    authors: Rodriguez Cruz PM,Huda S,López-Ruiz P,Vincent A

    更新日期:2015-08-01 00:00:00

  • Motor and somatosensory deficits following uni- and bilateral lesions of the cortex induced by aspiration or thermocoagulation in the adult rat.

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    authors: Napieralski JA,Banks RJ,Chesselet MF

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  • Mesenchymal stem cells and neuroregeneration in Parkinson's disease.

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    authors: Glavaski-Joksimovic A,Bohn MC

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  • Frequency specific activity in subthalamic nucleus correlates with hand bradykinesia in Parkinson's disease.

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    authors: Tan H,Pogosyan A,Anzak A,Foltynie T,Limousin P,Zrinzo L,Ashkan K,Bogdanovic M,Green AL,Aziz T,Brown P

    更新日期:2013-02-01 00:00:00

  • Characterization of histaminergic H3 receptors in intraocular tuberomammillary transplants containing histaminergic neurons.

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    authors: Bergman H,Prell GD,Lupica CR,Granholm AC

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    abstract::Oxaliplatin is first-line chemotherapy for colorectal cancer, but produces dose-limiting neurotoxicity. Acute neurotoxicity following infusion produces symptoms including cold-triggered fasciculations and cramps, with subsequent chronic neuropathy developing at higher cumulative doses. Axonal excitability studies were...

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    authors: Park SB,Lin CS,Krishnan AV,Goldstein D,Friedlander ML,Kiernan MC

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    pub_type: 杂志文章


    authors: Shinoda M,Hudson JL,Strömberg I,Hoffer BJ,Moorhead JW,Olson L

    更新日期:1996-10-01 00:00:00

  • Microglial and astroglial reactions to ischemic and kainic acid-induced lesions of the adult rat hippocampus.

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    authors: Jørgensen MB,Finsen BR,Jensen MB,Castellano B,Diemer NH,Zimmer J

    更新日期:1993-03-01 00:00:00

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  • NAD+ precursor modulates post-ischemic mitochondrial fragmentation and reactive oxygen species generation via SIRT3 dependent mechanisms.

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    authors: Klimova N,Fearnow A,Long A,Kristian T

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  • Volumetric changes in the anterior olfactory nucleus of the rat after neonatal olfactory deprivation.

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    authors: Barbado MV,Briñón JG,Weruaga E,Porteros A,Arévalo R,Aijón J,Alonso JR

    更新日期:2001-10-01 00:00:00

  • Dendrite bundles in motoneuronal pools of trunk and extremity muscles in the rat.

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    authors: Gramsbergen A,Ijkema-Paassen J,Westerga J,Geisler HC

    更新日期:1996-01-01 00:00:00

  • Progesterone and allopregnanolone reduce inflammatory cytokines after traumatic brain injury.

    abstract::Following a traumatic brain injury (TBI), the excessive release of interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) is a major cause of cerebral edema, which, in turn, can cause permanent neuronal loss and cognitive deficits in laboratory rats. This study examined the changes in expression of t...

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    authors: He J,Evans CO,Hoffman SW,Oyesiku NM,Stein DG

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  • Seizure prediction using scalp electroencephalogram.

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    journal_title:Experimental neurology

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    authors: Drury I,Smith B,Li D,Savit R

    更新日期:2003-11-01 00:00:00

  • Monosialoganglioside GM1 reduces NMDA neurotoxicity in neonatal rat brain.

    abstract::Monosialoganglioside GM1 prevents excitatory amino acid (EAA)-related neuronal death in cultured central nervous system (CNS) neurons and reduces the severity of acute brain damage in different experimental models of cerebral ischemia. Using a model of brain damage induced by intracerebroventricular administration of ...

    journal_title:Experimental neurology

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    authors: Lipartiti M,Lazzaro A,Zanoni R,Mazzari S,Toffano G,Leon A

    更新日期:1991-09-01 00:00:00

  • Inhibition of the accelerative effects of testosterone on hamster facial nerve regeneration by the antiandrogen flutamide.

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    authors: Kujawa KA,Tanzer L,Jones KJ

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  • Cutaneous inputs from the back abolish locomotor-like activity and reduce spastic-like activity in the adult cat following complete spinal cord injury.

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  • Postural development in rats.

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    authors: Lelard T,Jamon M,Gasc JP,Vidal PP

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  • Sleep deprivation attenuates experimental stroke severity in rats.

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    authors: Moldovan M,Constantinescu AO,Balseanu A,Oprescu N,Zagrean L,Popa-Wagner A

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    authors: Rajaona J,Dallel R,Woda A

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  • Alterations in voluntary movement execution in Huntington's disease are related to the dominant motor system: evidence from event-related potentials.

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    authors: Beste C,Konrad C,Saft C,Ukas T,Andrich J,Pfleiderer B,Hausmann M,Falkenstein M

    更新日期:2009-03-01 00:00:00

  • Cyclic AMP elevates tubulin expression without increasing intrinsic axon growth capacity.

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    authors: Han PJ,Shukla S,Subramanian PS,Hoffman PN

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  • Noradrenergic, serotonergic, and cholinergic sprouting in the hippocampus that follows partial or complete transection of the septohippocampal pathway: contributions of spared inputs.

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    authors: Gasser UE,Dravid AR

    更新日期:1987-05-01 00:00:00

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    abstract::GAP-43 is a neuronal protein whose synthesis is elevated during developmental and regenerative axon growth. We propose that one consequence of this increased synthesis may be the delivery of calmodulin-like proteins to the distal portions of the growing axon at an increased velocity; this is because calmodulin, which ...

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    authors: Spencer S,Willard MB

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