Abstract:
:PDCD4, the protein encoded by the tumor suppressor gene PDCD4 (programmed cell death 4) has been implicated in the control of cellular transcription and translation by modulating the activity of specific transcription factors and suppressing the translation of mRNAs with structured 5'-UTRs. Most studies of human PDCD4 have employed tumor cell lines, possibly resulting in a biased picture of its role in normal cells. Here, we have studied the function of PDCD4 in a telomerase-immortalized human epithelial cell line. We show for the first time that PDCD4 is required for the G1/S-transition, demonstrating its crucial role in the cell cycle. Inhibition of p53-dependent activation of p21WAF1/CIP1 overrides the requirement for PDCD4 for the G1/S-transition, suggesting that PDCD4 counteracts basal p53 activity to prevent activation of the G1/S checkpoint by p53. Transcriptome and ribosome profiling data show that silencing of PDCD4 changes the expression levels and translation of many mRNAs, providing an unbiased view of the cellular processes that are affected by PDCD4 in an epithelial cell line. Our data identify PDCD4 as a key regulator of cell cycle- and DNA-related functions that are inhibited when it is silenced, suggesting that decreased expression of PDCD4 might contribute to tumor development by compromising genomic integrity.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Haas A,Nilges BS,Leidel SA,Klempnauer KHdoi
10.1038/s41598-020-59678-wsubject
Has Abstractpub_date
2020-02-17 00:00:00pages
2758issue
1issn
2045-2322pii
10.1038/s41598-020-59678-wjournal_volume
10pub_type
杂志文章abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-34742-8
更新日期:2018-11-01 00:00:00
abstract::Hepatitis E Virus-like particles self-assemble in to noninfectious nanocapsids that are resistant to proteolytic/acidic mucosal delivery conditions. Previously, the nanocapsid was engineered to specifically bind and enter breast cancer cells, where successful tumor targeting was demonstrated in animal models. In the p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17171-x
更新日期:2017-12-06 00:00:00
abstract::Nowadays Field Emission Gun-Scanning Electron Microscopes provide detailed crystallographic information with high spatial and angular resolutions, and allow direct observation of crystalline defects, such as dislocations, through an attractive technique called Electron Channeling Contrast Imaging (ECCI). Dislocations ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09756-3
更新日期:2017-08-29 00:00:00
abstract::The biological processes involved in a drug's mechanisms of action are oftentimes dynamic, complex and difficult to discern. Time-course gene expression data is a rich source of information that can be used to unravel these complex processes, identify biomarkers of drug sensitivity and predict the response to a drug. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74725-2
更新日期:2020-10-19 00:00:00
abstract::Neonatal hypoxic-ischaemic (HI) encephalopathy is among the most serious complications in neonatology. In the present study, we studied the immediate (0 hour), subacute (36 hours) and late (144 hours) responses of the neonatal brain to experimental HI insult in laboratory rats. At the striatal level, the mass spectrom...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25088-2
更新日期:2018-05-03 00:00:00
abstract::The release of radon in active fault zones is a sustained radioactive pollution source of the atmospheric environment. The species, concentration and flux of radon emitted in soil gas in active fault zones in the Capital of China were investigated by in-situ field measurements. Two main species of radon discharging fr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35262-1
更新日期:2018-11-13 00:00:00
abstract::Jasmonates (JAs) play important roles in plant growth, development and defense. Comprehensive metabolomics profiling of plants under JA treatment provides insights into the interaction and regulation network of plant hormones. Here we applied high resolution mass spectrometry based metabolomics approach on Arabidopsis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37674
更新日期:2016-11-24 00:00:00
abstract::We fabricate high-performance, flexible, transparent electrochromic (EC) films and thin film heaters (TFHs) on an ITO/Cu/ITO (ICI) multilayer electrode prepared by continuous roll-to-roll (RTR) sputtering of ITO and Cu targets. The RTR-sputtered ICI multilayer on a 700 mm wide PET substrate at room temperature exhibit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33868
更新日期:2016-09-22 00:00:00
abstract::Early treatment of single sided deafness in children has been recommended to protect from neurodevelopmental preference for the better hearing ear and from social and educational deficits. A fairly homogeneous group of five young children (≤3.6 years of age) with normal right sided hearing who received a cochlear impl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17129-z
更新日期:2017-12-04 00:00:00
abstract::SNPs may restrict cell detoxification activity and be a potential risk factor for cancer chemosensitivity. We evaluated the predictive value of these polymorphisms on the sensitivity of bladder cancer patients to epirubicin and mitomycin chemotherapy instillation as well as their toxicities. SNPs were analyzed by TaqM...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14000
更新日期:2015-09-10 00:00:00
abstract::Apparently mundane, amorphous nanostructures of carbon have optical properties which are as exotic as their crystalline counterparts. In this work we demonstrate a simple and inexpensive mechano-chemical method to prepare bulk quantities of self-passivated, amorphous carbon dots. Like the graphene quantum dots, the wa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28021-9
更新日期:2018-06-27 00:00:00
abstract::Genome engineering is a rapidly evolving field that benefits from the availability of different tools that can be used to perform genome manipulation tasks. We describe here the development of the Flp-TAL recombinases that can target genomic FRT-like sequences in their native chromosomal locations. Flp-TAL recombinase...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74474-2
更新日期:2020-10-15 00:00:00
abstract::Synthetic analogues of mycobacterial trehalose-dimycolate such as trehalose acyl esters have been proposed as novel adjuvants for vaccination. They induce an immune response by binding to the macrophage C-type lectin receptor Mincle. The binding site of trehalose is known, but there is yet only very limited structural...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23624-8
更新日期:2018-03-29 00:00:00
abstract::Magnetic skyrmions are promising candidates for the next generation of spintronic devices due to their small size and topologically protected structure. One challenge for using these magnetic states in applications lies on controlling the nucleation process and stabilization that usually requires an external force. He...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40705-4
更新日期:2019-03-11 00:00:00
abstract::In adipose tissue, agonists of the β3-adrenergic receptor (ADRB3) regulate lipolysis, lipid oxidation, and thermogenesis. The deficiency in the thermogenesis induced by neuroblast differentiation-associated protein AHNAK in white adipose tissue (WAT) of mice fed a high-fat diet suggests that AHNAK may stimulate energy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23426
更新日期:2016-03-18 00:00:00
abstract::The side effects of cisplatin (CDDP), notably nephrotoxicity, greatly limited its use in clinical chemotherapy. HuangQi Injections (HI), a commonly used preparation of the well-known Chinese herbal medicine Astragali radix, appeared to be promising treatment for nephrotoxicity without compromising the anti-tumor activ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03249-z
更新日期:2017-06-15 00:00:00
abstract::Inversely tapered silicon photonic resonators on silicon substrates were shown to host multiple high-Q whispering gallery modes and constitute versatile building blocks for CMOS compatible solid state lighting, optical sensing and modulator devices. So far, numerical analyses by the finite difference time domain metho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45034-0
更新日期:2019-06-21 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-58752-7
更新日期:2020-01-30 00:00:00
abstract::Ample evidence indicates that obesity causes dysfunctions in the lung. Previous studies also show that cholinergic anti-inflammatory pathways play crucial roles in obesity-induced chronic inflammation via α7 nicotinic acetylcholine receptor (α7nAChR) signaling. However, it remains unclear whether and how obesity affec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75414-w
更新日期:2020-10-27 00:00:00
abstract::We investigated gender differences in the optic nerve head (ONH) microcirculation status in association with obstructive sleep apnea (OSA) by using laser speckle flowgraphy (LSFG). We evaluated 150 men (60.5 ± 11.0 yrs) and 45 women (63.0 ± 10.6 yrs) who underwent overnight polysomnography. The mean blur rate (MBR), m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55470-7
更新日期:2019-12-11 00:00:00
abstract::Metastasis causes approximately 90% of breast cancer-related deaths in women. Previously, we have demonstrated that 2-dodecyl-6-methoxycyclohexa-2,5-diene- 1,4-dione (DMDD) remarkably inhibited the growth of human breast cancer cells with little toxicity. In this study, we investigated the toxicity and efficacy of DMD...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07162-3
更新日期:2017-07-27 00:00:00
abstract::The decomposition of methane (CH4) is a catalytically important reaction in the production of syngas that is used to make a wide spectrum of hydrocarbons and alcohols, and a principal carbon deposition pathway in methane reforming. Literatures suggest that stepped Ni surface is uniquely selective toward methane decomp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14050-3
更新日期:2017-10-25 00:00:00
abstract::Cardiac progenitor cells (CPCs) have been shown to promote cardiac regeneration and improve heart function. However, evidence suggests that their regenerative capacity may be limited in conditions of severe hypoxia. Elucidating the mechanisms involved in CPC protection against hypoxic stress is essential to maximize t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09813-x
更新日期:2017-08-29 00:00:00
abstract::Traumatic brain injury (TBI) is a risk factor for Alzheimer's disease (AD), although the mechanisms contributing to this increased risk are unknown. Insulin resistance is an additional risk factor for AD whereby decreased insulin signaling increases synaptic sensitivity to amyloid beta (Aβ) and tau. Considering this, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44635-z
更新日期:2019-06-03 00:00:00
abstract::Textile forms of solar cells possess special advantages over other types of solar cells, including their light weight, high flexibility, and mechanical robustness. Recent demand for wearable devices has promoted interest in the development of high-efficiency textile-based solar cells for energy suppliers. However, the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05322
更新日期:2014-06-24 00:00:00
abstract::High-throughput sequencing was applied to analyze the effects of toxic Microcystis aeruginosa on the silver carp Hypophthalmichthys molitrix. Silver carps were exposed to two cyanobacteria species (toxic and non-toxic) for RNA-seq and miRNA-seq analysis. RNA-seq revealed that the liver tissue contained 105,379 unigene...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10335-9
更新日期:2017-09-05 00:00:00
abstract::The calcifying shell is an excellent model for studying biomineralization and evolution. However, the molecular mechanisms of shell formation are only beginning to be elucidated in Mollusca. It is known that shell matrix proteins (SMPs) play important roles in shell formation. With increasing data of shell matrix prot...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45754
更新日期:2017-04-04 00:00:00
abstract::Metabolic syndrome (MS) could be associated with liver function. Our study aimed to investigate the association between liver function and MS in a large cohort of Chinese men and women. We enrolled 32,768 ostensibly healthy participants. The associations between liver function and MS of both genders were analyzed sepa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44844
更新日期:2017-03-20 00:00:00
abstract::Quantitatively determining physiological parameters at a microscopic level in the retina furthers the understanding of the molecular pathways of blinding diseases, such as diabetic retinopathy and glaucoma. An essential parameter, which has yet to be quantified noninvasively, is the retinal oxygen metabolic rate (rMRO...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06525
更新日期:2014-10-06 00:00:00
abstract::Securin overexpression correlates with poor prognosis in various tumours. We have previously shown that securin depletion promotes radiation-induced senescence and enhances radiosensitivity in human cancer cells. However, the underlying molecular mechanisms and the paracrine effects remain unknown. In this study, we s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01675
更新日期:2013-01-01 00:00:00