Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion.


:Prestin (SLC26a5) is an integral membrane motor protein in outer hair cells (OHC) that underlies cochlear amplification. As a voltage-dependent protein, it relies on intrinsic sensor charge to respond to transmembrane voltage (receptor potentials), thereby effecting conformational changes. The protein's electromechanical actively is experimentally monitored as a bell-shaped nonlinear capacitance (NLC), whose magnitude peaks at a characteristic voltage, Vh. This voltage denotes the midpoint of prestin's charge-voltage (Q-V) Boltzmann distribution and region of maximum gain of OHC electromotility. It is an important factor in hearing capabilities for mammals. A variety of biophysical forces can influence the distribution of charge, gauged by shifts in Vh, including prior holding voltage or membrane potential. Here we report that the effectiveness of prior voltage augments during the delivery of prestin to the membranes in an inducible HEK cell line. The augmentation coincides with an increase in prestin density, maturing at a characteristic membrane areal density of 870 functional prestin units per square micrometer, and is likely indicative of prestin-prestin cooperative interactions.






Zhai F,Song L,Bai JP,Dai C,Navaratnam D,Santos-Sacchi J




Has Abstract


2020-04-01 00:00:00












  • Spinal matrix metalloproteinase 3 mediates inflammatory hyperalgesia via a tumor necrosis factor-dependent mechanism.

    abstract::Matrix metalloproteinases (MMPs) have been implicated in the modulation of synaptic plasticity, glial activation, and long-term potentiation in the CNS. Here we demonstrate for the first time a mechanism for the regulation of nociceptive processing by spinal MMP-3 during peripheral inflammation. We first determined by...


    pub_type: 杂志文章


    authors: Christianson CA,Fitzsimmons BL,Shim JH,Agrawal A,Cohen SM,Hua XY,Yaksh TL

    更新日期:2012-01-03 00:00:00

  • Inactivation of large-conductance, calcium-activated potassium channels in rat cortical neurons.

    abstract::Inside-out patch recordings from rat acutely dissociated cerebral cortical neurons revealed time and voltage-dependent activity of a large-conductance calcium-activated potassium channel. Channel activity inactivated within minutes following a depolarizing voltage step, and was recovered from inactivation by membrane ...


    pub_type: 杂志文章


    authors: Smith MA,Ashford ML

    更新日期:2000-01-01 00:00:00

  • Chronic continuous and intermittent L-3,4-dihydroxyphenylalanine treatments differentially affect basal ganglia function in 6-hydroxydopamine lesioned rats--an autoradiographic study using [3H]flunitrazepam.

    abstract::The effects of chronic 'continuous' and 'intermittent' L-3,4-dihydroxyphenylalanine treatments on GABA receptor function in the basal ganglia of rats with unilateral 6-hydroxydopamine lesions of the medial forebrain bundle was investigated, by autoradiography with [3H]flunitrazepam. The 6-hydroxydopamine lesion itself...


    pub_type: 杂志文章


    authors: Gnanalingham KK,Robertson RG

    更新日期:1993-12-01 00:00:00

  • 5-Lipoxygenase in mouse cerebellar Purkinje cells.

    abstract::It has been suggested that the enzymatic pathway of 5-lipoxygenase (5-LOX) influences brain functioning and pathobiology. The mRNAs for both the enzyme 5-LOX and its activating protein FLAP have been found in the cerebellum. In this work, we investigated the cellular expression of 5-LOX in the adult mouse cerebellar c...


    pub_type: 杂志文章


    authors: Chen H,Dzitoyeva S,Manev H

    更新日期:2010-12-01 00:00:00

  • gp130 cytokines, leukemia inhibitory factor and interleukin-6, induce neuropeptide expression in intact adult rat sensory neurons in vivo: time-course, specificity and comparison with sciatic nerve axotomy.

    abstract::The gp130 cytokines leukemia inhibitory factor and interleukin-6 are neuroactive cytokines associated with peripheral nerve injury. Here we show that exogenous administration of these factors selectively regulates neuropeptide phenotype in intact sensory neurons in a manner consistent with their role as injury-induced...


    pub_type: 杂志文章


    authors: Thompson SW,Priestley JV,Southall A

    更新日期:1998-06-01 00:00:00

  • Fast, synchronous neurotransmitter release: Past, present and future.

    abstract::This mini-review starts with a summary of the crucial contributions Ricardo Miledi made to our understanding of how the action potential triggers fast, synchronous transmitter release. It then transitions to the discovery of synaptotagmin and its role as the exocytotic Ca2+ sensor at nerve terminals. The final section...


    pub_type: 杂志文章,评审


    authors: Gundersen CB

    更新日期:2020-07-15 00:00:00

  • Analysis of purinergic and cholinergic fast synaptic transmission to identified myenteric neurons.

    abstract::Types and projections of neurons that received cholinergic, purinergic and other fast excitatory synaptic inputs in myenteric ganglia of the guinea-pig distal colon were identified using combined electrophysiological recording, application of selective antagonists, marker dye filling via the recording microelectrode, ...


    pub_type: 杂志文章


    authors: Nurgali K,Furness JB,Stebbing MJ

    更新日期:2003-01-01 00:00:00

  • Effects of voluntary exercise on hippocampal long-term potentiation in morphine-dependent rats.

    abstract::This study was designed to examine the effect of voluntary exercise on hippocampal long-term potentiation (LTP) in morphine-dependent rats. The rats were randomly distributed into the saline-sedentary (Sal/Sed), the dependent-sedentary, the saline-exercise (Sal/Exc), and the dependent-exercise (D/Exc) groups. The Sal/...


    pub_type: 杂志文章


    authors: Miladi-Gorji H,Rashidy-Pour A,Fathollahi Y,Semnanian S,Jadidi M

    更新日期:2014-01-03 00:00:00

  • Kainate-induced seizures alter protein composition and N-methyl-D-aspartate receptor function of rat forebrain postsynaptic densities.

    abstract::The postsynaptic density is a highly dynamic structure, which is reorganized in an activity-dependent manner. An animal model for temporal lobe epilepsy, i.e. kainate-induced limbic seizures in rats, was used to study changes in postsynaptic density composition after extensive synaptic activity. Six hours after kainat...


    pub_type: 杂志文章


    authors: Wyneken U,Smalla KH,Marengo JJ,Soto D,de la Cerda A,Tischmeyer W,Grimm R,Boeckers TM,Wolf G,Orrego F,Gundelfinger ED

    更新日期:2001-01-01 00:00:00

  • Electron microscopic investigation of the vestibular projection to the cat trochlear nuclei.

    abstract::Ultrastructural degeneration studies were carried out on the cat trochlear nucleus following lesion of the vestibulo-trochlear pathway in order to characterize the location and type of presynaptic endings involved in this pathway. Four types of boutons are found in the normal trochlear nucleus. Types I and II are larg...


    pub_type: 杂志文章


    authors: Bak IJ,Baker R,Choi WB,Precht W

    更新日期:1976-12-01 00:00:00

  • Activation of both Group I and Group II metabotropic glutamatergic receptors suppress retinogeniculate transmission.

    abstract::Relay cells of dorsal lateral geniculate nucleus (LGN) receive a Class 1 glutamatergic input from the retina and a Class 2 input from cortical layer 6. Among the properties of Class 2 synapses is the ability to activate metabotropic glutamate receptors (mGluRs), and mGluR activation is known to affect thalamocortical ...


    pub_type: 杂志文章


    authors: Lam YW,Sherman SM

    更新日期:2013-07-09 00:00:00

  • Time-course of hippocampal granule cell degeneration and changes in adult neurogenesis after adrenalectomy in rats.

    abstract::The hippocampus maintains the remarkable ability to generate new neurons throughout the lifespan. Progenitor cells in the subgranular zone give rise mainly to granule cells that migrate to the granule cell layer and become mature, functionally integrated neurons. Numerous factors are capable of regulating the prolifer...


    pub_type: 杂志文章


    authors: Spanswick SC,Epp JR,Sutherland RJ

    更新日期:2011-09-08 00:00:00

  • 3-Amino thioacridone, a selective cyclin-dependent kinase 4 inhibitor, attenuates kainic acid-induced apoptosis in neurons.

    abstract::The mechanisms underlying selective neuronal cell death in kainic acid-mediated neurodegeneration are not fully understood. We have recently demonstrated that in cerebellar granule neurons, kainic acid induces the expression of proteins associated with cell-cycle progression. In the present study we show that 3-amino ...


    pub_type: 杂志文章


    authors: Verdaguer E,Jordà EG,Canudas AM,Jiménez A,Sureda FX,Rimbau V,Pubill D,Escubedo E,Camarasa J,Pallàs M,Camins A

    更新日期:2003-01-01 00:00:00

  • Functional recovery of stepping in rats after a complete neonatal spinal cord transection is not due to regrowth across the lesion site.

    abstract::Rats receiving a complete spinal cord transection (ST) at a neonatal stage spontaneously can recover significant stepping ability, whereas minimal recovery is attained in rats transected as adults. In addition, neonatally spinal cord transected rats trained to step more readily improve their locomotor ability. We hypo...


    pub_type: 杂志文章


    authors: Tillakaratne NJ,Guu JJ,de Leon RD,Bigbee AJ,London NJ,Zhong H,Ziegler MD,Joynes RL,Roy RR,Edgerton VR

    更新日期:2010-03-10 00:00:00

  • Endopeptidase-24.11 is suppressed in myelin-forming but not in non-myelin-forming Schwann cells during development of the rat sciatic nerve.

    abstract::Endopeptidase-24.11, which is identical with the common acute lymphoblastic leukemia antigen (CALLA), is a cell surface zinc metalloprotease that has the ability to hydrolyse a variety of physiologically active peptides. Interest in this enzyme is based on the view that it may play a role in the regulation of peptide ...


    pub_type: 杂志文章


    authors: Kioussi C,Crine P,Matsas R

    更新日期:1992-09-01 00:00:00

  • Inhibition of protein phosphatase 2A- and protein phosphatase 1-induced tau hyperphosphorylation and impairment of spatial memory retention in rats.

    abstract::Tau hyperphosphorylation leads to formation of paired helical filament/neurofibrillary tangles, the hallmark lesion seen in Alzheimer's disease (AD) brain. An imbalanced regulation in protein kinases and protein phosphatases in the affected neurons is proposed to be a reasonable causative factor to the disease process...


    pub_type: 杂志文章


    authors: Sun L,Liu SY,Zhou XW,Wang XC,Liu R,Wang Q,Wang JZ

    更新日期:2003-01-01 00:00:00

  • Short-term deep brain stimulation of the thalamic reticular nucleus modifies aberrant oscillatory activity in a neurodevelopment model of schizophrenia.

    abstract::Dysfunction of thalamo-cortical networks involving particularly the thalamic reticular nucleus (TRN) is implicated in schizophrenia. In the neonatal ventral hippocampal lesion (NVHL), a heuristic animal model of schizophrenia, brain oscillation changes similar to those of schizophrenic patients have been reported. The...


    pub_type: 杂志文章


    authors: Magdaleno-Madrigal VM,Contreras-Murillo G,Camacho-Abrego I,Negrete-Díaz JV,Valdés-Cruz A,Fernández-Mas R,Almazán-Alvarado S,Flores G

    更新日期:2017-08-15 00:00:00

  • Glial fibrillary acidic protein mRNA levels in the cingulate cortex of individuals with depression, bipolar disorder and schizophrenia.

    abstract::Recent studies have shown a decrease in glial number and glial fibrillary acidic protein (GFAP) levels in the frontal and cingulate cortices of individuals with mood disorders and schizophrenia. In an attempt to verify and expand these findings we examined GFAP messenger ribonucleic acid (mRNA) levels in postmortem se...


    pub_type: 杂志文章


    authors: Webster MJ,O'Grady J,Kleinman JE,Weickert CS

    更新日期:2005-01-01 00:00:00

  • The relation between transmitter release and Ca2+ entry at the mouse motor nerve terminal: role of stochastic factors causing heterogeneity.

    abstract::The relation between quantal transmitter release and presynaptic Ca2+/Ba2+ entry at the mouse neuromuscular junction was studied, making use of the finding that in the presence of Ba2+ trains of nerve stimuli or brief nerve terminal depolarizations elicit "tails" of raised miniature end-plate potential frequency (fm) ...


    pub_type: 杂志文章


    authors: Quastel DM,Guan YY,Saint DA

    更新日期:1992-12-01 00:00:00

  • Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons from apoptosis.

    abstract::Novel approaches are required in the clinical management of peripheral nerve injuries because current surgical techniques result in deficient sensory recovery. Microsurgery alone fails to address extensive cell death in the dorsal root ganglia (DRG), in addition to poor axonal regeneration. Incorporation of cultured c...


    pub_type: 杂志文章


    authors: Reid AJ,Sun M,Wiberg M,Downes S,Terenghi G,Kingham PJ

    更新日期:2011-12-29 00:00:00

  • Aging-Related Oxidative Stress: Positive Effect of Memory Training.

    abstract::The cognitive impairment characterizing the phenotype of older adults has been related to the efficiency of the antioxidant system. This study aimed at investigating the effect of memory training (MT) on memory, global cognitive functioning, and the oxidant and antioxidant capacity of plasma. We recruited 52 healthy s...


    pub_type: 杂志文章


    authors: Pesce M,Tatangelo R,La Fratta I,Rizzuto A,Campagna G,Turli C,Ferrone A,Franceschelli S,Speranza L,Patruno A,Ballerini P,De Lutiis MA,Felaco M,Grilli A

    更新日期:2018-02-01 00:00:00

  • Anti-glutamatergic effect of riluzole: comparison with valproic acid.

    abstract::Riluzole, an anti-amyotrophic lateral sclerosis drug, known to decrease presynaptic glutamate release, is viewed as a candidate supplementary medication for epilepsy. In the present study, we compared the effects of riluzole and valproate (VPA) in the pilocarpine-induced limbic seizure model and in the gamma-hydroxybu...


    pub_type: 杂志文章


    authors: Kim JE,Kim DS,Kwak SE,Choi HC,Song HK,Choi SY,Kwon OS,Kim YI,Kang TC

    更新日期:2007-06-15 00:00:00

  • Differential effects of prenatal stress on the morphological maturation of hippocampal neurons.

    abstract::The present study was designed to clarify an intensity-dependent effect of prenatal stress on the morphological development of hippocampal neurons in rats. In addition, the involvement of receptors for glucocorticoids, i.e. mineralocorticoid receptors and glucocorticoid receptors, in stress-induced changes in the morp...


    pub_type: 杂志文章


    authors: Fujioka A,Fujioka T,Ishida Y,Maekawa T,Nakamura S

    更新日期:2006-08-25 00:00:00

  • Ankyrin immunoreactivity in adult rat cerebellum.

    abstract::An antibody recognizing all ankyrin isoforms was employed to localize ankyrin in the cerebellum by light and electron microscopy. White matter (myelinated fiber tracts) did not contain ankyrin. Granule cell bodies and axons contained ankyrin which extended into the parallel fibers and their synapses. Purkinje cells co...


    pub_type: 杂志文章


    authors: Babitch JA

    更新日期:1993-06-01 00:00:00

  • C6 glioma cells differentiated by retinoic acid overexpress the glutamate transporter excitatory amino acid carrier 1 (EAAC1).

    abstract::The transport of excitatory amino acids (EAA) in CNS is performed by a family of high affinity, sodium dependent carriers. One of these transporters, excitatory amino acid carrier 1 (EAAC1), is known to be regulated by several mechanisms that modify carrier abundance on the plasma membrane. Much less is known on EAAC1...


    pub_type: 杂志文章


    authors: Bianchi MG,Gazzola GC,Tognazzi L,Bussolati O

    更新日期:2008-02-19 00:00:00

  • Origins and pathways of cerebrovascular nerves storing substance P and calcitonin gene-related peptide in rat.

    abstract::Origins and pathways of cerebrovascular substance P- and calcitonin gene-related peptide-positive nerves in rat were studied by immunohistochemistry combined with denervation experiments and retrograde axonal tracer technique. The two peptides have been found to coexist in one and the same neuron. After sectioning of ...


    pub_type: 杂志文章


    authors: Suzuki N,Hardebo JE,Owman C

    更新日期:1989-01-01 00:00:00

  • Dopamine profoundly suppresses excitatory transmission in neonatal rat hippocampus via phosphatidylinositol-linked D1-like receptor.

    abstract::Dopamine modulates synaptic transmission in various brain regions. The disorder of dopamine system may be related to neurodevelopmental dysfunction. However, the action of dopamine on synaptic transmission during development is largely unknown. We studied the effect of dopamine on GABAergic and glutamatergic transmiss...


    pub_type: 杂志文章


    authors: Noriyama Y,Ogawa Y,Yoshino H,Yamashita M,Kishimoto T

    更新日期:2006-01-01 00:00:00

  • The mossy fiber projection of the nucleus reticularis tegmenti pontis to the flocculus and adjacent ventral paraflocculus in the cat.

    abstract::The pontine projection of the flocculus and adjacent ventral paraflocculus was investigated with antegrade and retrograde axonal tracer techniques. Injections of horseradish peroxidase into the floccular complex revealed subsets of labeled neurons in the nucleus reticularis tegmenti pontis, the nucleus raphe pontis an...


    pub_type: 杂志文章


    authors: Gerrits NM,Epema AH,Voogd J

    更新日期:1984-03-01 00:00:00

  • Changes in ensemble activity of hippocampus CA1 neurons induced by chronic morphine administration in freely behaving mice.

    abstract::The hippocampus plays an important role in the formation of new memories and spatial navigation. Recently, growing evidence supports the view that it is also involved in addiction to opiates and other drugs. Theoretical and experimental studies suggest that hippocampal neural-network oscillations at specific frequenci...


    pub_type: 杂志文章


    authors: Liu F,Jiang H,Zhong W,Wu X,Luo J

    更新日期:2010-12-15 00:00:00

  • Infralimbic cortex activation increases c-Fos expression in intercalated neurons of the amygdala.

    abstract::Recently, it was reported that stimulation of the infralimbic cortex produces a feedforward inhibition of central amygdala neurons. The interest of this observation comes from the fact that the central nucleus is the main output station of the amygdala for conditioned fear responses and evidence that the infralimbic c...


    pub_type: 杂志文章


    authors: Berretta S,Pantazopoulos H,Caldera M,Pantazopoulos P,Paré D

    更新日期:2005-01-01 00:00:00