Scanning laser optical tomography resolves developmental neurotoxic effects on pioneer neurons.

Abstract:

:Developmental neurotoxic compounds impair the developing human nervous system at lower doses than those affecting adults. Standardized test methods for assessing developmental neurotoxicity (DNT) require the use of high numbers of laboratory animals. Here, we use a novel assay that is based on the development of an intact insect embryo in serum-free culture. Neural pathways in the leg of embryonic locusts are established by a pair of afferent pioneer neurons, extending axons along a well-defined pathway to the central nervous system. After exposure to test chemicals, we analyze pioneer neuron shape with conventional fluorescence microscopy and compare it to 3D images, obtained by scanning laser optical tomography (SLOT) and processed by a segmentation algorithm. The segmented SLOT images resolve the 3D structure of the pioneers, recognize pathfinding defects and are thus advantageous for detecting DNT-positive compounds. The defects in axon elongation and pathfinding of pioneer axons caused by two DNT-positive reference compounds (methylmercury chloride; sodium(meta)arsenite) are compared to the biochemically measured general viability of the embryo. Using conventional fluorescence microscopy to establish concentration-response curves of axon elongation, we show that this assay identifies methylmercury chloride and the pro-apoptotic compound staurosporine as developmental neurotoxicants.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Bode K,Nolte L,Kamin H,Desens M,Ulmann A,Bergmann GA,Betker P,Reitmeier J,Ripken T,Stern M,Meyer H,Bicker G

doi

10.1038/s41598-020-59562-7

subject

Has Abstract

pub_date

2020-02-14 00:00:00

pages

2641

issue

1

issn

2045-2322

pii

10.1038/s41598-020-59562-7

journal_volume

10

pub_type

杂志文章
  • Sputum Proteomics Reveals a Shift in Vitamin D-binding Protein and Antimicrobial Protein Axis in Tuberculosis Patients.

    abstract::Existing understanding of molecular composition of sputum and its role in tuberculosis patients is variously limited to its diagnostic potential. We sought to identify infection induced sputum proteome alteration in active/non tuberculosis patients (A/NTB) and their role in altered lung patho-physiology. Out of the st...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37662-9

    authors: Bishwal SC,Das MK,Badireddy VK,Dabral D,Das A,Mahapatra AR,Sahu S,Malakar D,Singh II,Mazumdar H,Patgiri SJ,Deka T,Kapfo W,Liegise K,Kupa RU,Debnath S,Bhowmik R,Debnath R,Behera RK,Pillai MG,Deuri P,Nath R,Khal

    更新日期:2019-01-31 00:00:00

  • Bankruptcy is an inevitable fate of repeated investments with leverage.

    abstract::Due to the globalization and computerization of financial and economic activities, numerous repetitive leveraged investments have become possible in stock markets and currency exchanges. In reality, repeated leveraged investments up to 100 times/day are possible via online access. With computer-aided programs, this re...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50237-6

    authors: Nii M,Okabe T,Ito H,Morita S,Yasuda Y,Yoshimura J

    更新日期:2019-09-24 00:00:00

  • Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells.

    abstract::We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-18034-1

    authors: Badraiq H,Cvoro A,Galleu A,Simon M,Miere C,Hobbs C,Schulz R,Siow R,Dazzi F,Ilic D

    更新日期:2017-12-14 00:00:00

  • Chronic fetal exposure to caffeine altered resistance vessel functions via RyRs-BKCa down-regulation in rat offspring.

    abstract::Caffeine modifies vascular/cardiac contractility. Embryonic exposure to caffeine altered cardiac functions in offspring. This study determined chronic influence of prenatal caffeine on vessel functions in offspring. Pregnant Sprague-Dawley rats (5-month-old) were exposed to high dose of caffeine, their offspring (5-mo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13225

    authors: Li N,Li Y,Gao Q,Li D,Tang J,Sun M,Zhang P,Liu B,Mao C,Xu Z

    更新日期:2015-08-17 00:00:00

  • Swimming capability of zebrafish is governed by water temperature, caudal fin length and genetic background.

    abstract::Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological and behavioral traits. Motor traits, however, have not been explored in zebrafish strains. We here applied a treadmill for fish (swimmill) an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52592-w

    authors: Wakamatsu Y,Ogino K,Hirata H

    更新日期:2019-11-08 00:00:00

  • One-year Survey of human enteroviruses from sewage and the factors affecting virus adsorption to the suspended solids.

    abstract::This study described the results of environmental enterovirus surveillance conducted in Shandong Province of China in 2013. Altogether 39 sewage samples were collected and 873 enterovirus isolates (including 334 polioviruses) belonging to 22 serotypes were obtained. Echovirus (E) -7, coxsackievirus (CV) -B5, E-11, E-6...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31474

    authors: Tao Z,Wang Z,Lin X,Wang S,Wang H,Yoshida H,Xu A,Song Y

    更新日期:2016-08-11 00:00:00

  • Activin A is increased in the nucleus accumbens following a cocaine binge.

    abstract::Drug addiction is a long-lasting disease characterized by compulsive drug intake mediated in part by neuronal and biological adaptations in key brain areas, such as the nucleus accumbens (NAc). While we previously demonstrated involvement of the activin 2a receptor in drug taking, the role of its ligand, activin A, in...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43658

    authors: Wang ZJ,Martin JA,Gancarz AM,Adank DN,Sim FJ,Dietz DM

    更新日期:2017-03-08 00:00:00

  • Camouflaged Nanosilver with Excitation Wavelength Dependent High Quantum Yield for Targeted Theranostic.

    abstract::The present study shows the thorough investigations on optical properties and hydrodynamic diameters of glutathione (GSH) stabilized nanosilver clusters (AgNC) at different stages of synthesis and engineering for the optimized absolute quantum yield to generate fluorescent images of Dalton Lymphoma Ascites (DLA) tumou...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-34843-4

    authors: Girigoswami A,Yassine W,Sharmiladevi P,Haribabu V,Girigoswami K

    更新日期:2018-11-07 00:00:00

  • Sodium Ion Conductivity in Superionic IL-Impregnated Metal-Organic Frameworks: Enhancing Stability Through Structural Disorder.

    abstract::Metal-organic frameworks (MOFs) are intriguing host materials in composite electrolytes due to their ability for tailoring host-guest interactions by chemical tuning of the MOF backbone. Here, we introduce particularly high sodium ion conductivity into the zeolitic imidazolate framework ZIF-8 by impregnation with the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60198-w

    authors: Nozari V,Calahoo C,Tuffnell JM,Adelhelm P,Wondraczek K,Dutton SE,Bennett TD,Wondraczek L

    更新日期:2020-02-26 00:00:00

  • Preclinical evaluation of ribociclib and its synergistic effect in combination with alpelisib in non-keratinizing nasopharyngeal carcinoma.

    abstract::Ribociclib is a specific cyclin dependent kinase (Cdk) 4/6 inhibitor that induces G1 arrest by blocking the formation of cyclin D1-Cdk4/6 complex and inhibiting retinoblastoma (RB) phosphorylation. Cyclin D1 is overexpressed in over 90% of nasopharyngeal carcinoma (NPC) and CCND1 gene activation plays a critical role ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26201-1

    authors: Wong CH,Ma BBY,Hui CWC,Lo KW,Hui EP,Chan ATC

    更新日期:2018-05-22 00:00:00

  • Development of fully automated and ultrasensitive assays for urinary adiponectin and their application as novel biomarkers for diabetic kidney disease.

    abstract::Glomerular filtration rate (GFR) and urinary albumin excretion rate (UAER) are used to diagnose and classify the severity of chronic kidney disease. Total adiponectin (T-AN) and high molecular weight adiponectin (H-AN) assays were developed using the fully automated immunoassay system, HI-1000 and their significance o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72494-6

    authors: Watanabe T,Fujimoto Y,Morimoto A,Nishiyama M,Kawai A,Okada S,Aiba M,Kawano T,Kawahigashi M,Ishizu M,Mori H,Matsuhisa M,Hata A,Funaki M,Hashida S

    更新日期:2020-09-28 00:00:00

  • Precipitation behaviour during the β → α/ω phase transformation and its effect on the mechanical performance of a Ti-15Mo-2.7Nb-3Al-0.2Si alloy.

    abstract::The activation energy of the β → α/ω phase transformation increased monotonously with the application of a continuous heating process to a Ti-15Mo-2.7Nb-3Al-0.2Si alloy. Precipitation behaviour of the alloy aged at different temperatures were analysed with scanning electron microscopy, electron backscattered diffracti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54114-0

    authors: Xu T,Zhang S,Liang S,Cui N,Cao L,Wan Y

    更新日期:2019-11-26 00:00:00

  • Author Correction: Preparation and antimicrobial activity of ZnO-NPs coated cotton/starch and their functionalized ZnO-Ag/cotton and Zn(II) curcumin/cotton materials.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-65025-w

    authors: El-Nahhal IM,Salem J,Anbar R,Kodeh FS,Elmanama A

    更新日期:2020-05-27 00:00:00

  • Intravenous infusion of human bone marrow mesenchymal stromal cells promotes functional recovery and neuroplasticity after ischemic stroke in mice.

    abstract::Transplantation of human bone marrow mesenchymal stromal cells (hBM-MSC) promotes functional recovery after stroke in animal models, but the mechanisms underlying these effects remain incompletely understood. We tested the efficacy of Good Manufacturing Practices (GMP) compliant hBM-MSC, injected intravenously 3.5 hou...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07274-w

    authors: Sammali E,Alia C,Vegliante G,Colombo V,Giordano N,Pischiutta F,Boncoraglio GB,Barilani M,Lazzari L,Caleo M,De Simoni MG,Gaipa G,Citerio G,Zanier ER

    更新日期:2017-07-31 00:00:00

  • Bi-specific molecule against EGFR and death receptors simultaneously targets proliferation and death pathways in tumors.

    abstract::Developing therapeutics that target multiple receptor signaling pathways in tumors is critical as therapies targeting single specific biomarker/pathway have shown limited efficacy in patients with cancer. In this study, we extensively characterized a bi-functional molecule comprising of epidermal growth factor recepto...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02483-9

    authors: Zhu Y,Bassoff N,Reinshagen C,Bhere D,Nowicki MO,Lawler SE,Roux J,Shah K

    更新日期:2017-06-01 00:00:00

  • Molecular evolution of coxsackievirus A24v in Cuba over 23-years, 1986-2009.

    abstract::Coxsackievirus A24 variant (CVA24v) is a major causative agent of acute hemorrhagic conjunctivitis outbreaks worldwide, yet the evolutionary and transmission dynamics of the virus remain unclear. To address this, we analyzed and compared the 3C and partial VP1 gene regions of CVA24v isolates obtained from five outbrea...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70436-w

    authors: Fonseca MC,Pupo-Meriño M,García-González LA,Resik S,Hung LH,Muné M,Rodríguez H,Morier L,Norder H,Sarmiento L

    更新日期:2020-08-13 00:00:00

  • LFP beta amplitude is linked to mesoscopic spatio-temporal phase patterns.

    abstract::Beta oscillations observed in motor cortical local field potentials (LFPs) recorded on separate electrodes of a multi-electrode array have been shown to exhibit non-zero phase shifts that organize into planar waves. Here, we generalize this concept to additional classes of salient patterns that fully describe the spat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22990-7

    authors: Denker M,Zehl L,Kilavik BE,Diesmann M,Brochier T,Riehle A,Grün S

    更新日期:2018-03-26 00:00:00

  • Increased lead concentrations in the hairs of radiographers in general hospitals.

    abstract::This study investigated lead concentrations in the hairs of radiographers working in the radiological departments of general hospitals that used lead shielding for radiation protection. We collected scalp hair samples from 32 radiographers working in four radiology departments with lead shielding and 18 administration...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80721-3

    authors: Hung MC,Chang P

    更新日期:2021-01-08 00:00:00

  • Engineered Dendritic Cell-Directed Concurrent Activation of Multiple T cell Inhibitory Pathways Induces Robust Immune Tolerance.

    abstract::Inhibitory/repressor-receptors are upregulated significantly on activated T cells, and have been the molecules of attention as targets for inducing immune tolerance. Induction of effective antigen specific tolerance depends on concurrent engagement of the TCR and one or more of these inhibitory receptors. Here, we sho...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48464-y

    authors: Gudi RR,Karumuthil-Melethil S,Perez N,Li G,Vasu C

    更新日期:2019-08-19 00:00:00

  • Causes of volcanic unrest at Mt. Spurr in 2004-2005 inferred from repeated tomography.

    abstract::Mt. Spurr is the largest active volcano in Alaska of high explosive potential. The most recent activity, including two recent magmatic eruptions in 1953 and 1992, has occurred via the flanking Crater Peak. From 2004 to 2006, strong seismicity, gas flux, and heating were observed in the summit area, which had remained ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35453-w

    authors: Koulakov I,Smirnov SZ,Gladkov V,Kasatkina E,West M,El Khrepy S,Al-Arifi N

    更新日期:2018-11-30 00:00:00

  • Towards a Physarum learning chip.

    abstract::Networks of protoplasmic tubes of organism Physarum polycehpalum are macro-scale structures which optimally span multiple food sources to avoid repellents yet maximize coverage of attractants. When data are presented by configurations of attractants and behaviour of the slime mould is tuned by a range of repellents, t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19948

    authors: Whiting JG,Jones J,Bull L,Levin M,Adamatzky A

    更新日期:2016-02-03 00:00:00

  • Optimal Universal Uncertainty Relations.

    abstract::We study universal uncertainty relations and present a method called joint probability distribution diagram to improve the majorization bounds constructed independently in [Phys. Rev. Lett. 111, 230401 (2013)] and [J. Phys. A. 46, 272002 (2013)]. The results give rise to state independent uncertainty relations satisfi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep35735

    authors: Li T,Xiao Y,Ma T,Fei SM,Jing N,Li-Jost X,Wang ZX

    更新日期:2016-10-24 00:00:00

  • Immune Responses Induced by Recombinant Bacillus Subtilis Expressing the Hemagglutinin Protein of H5N1 in chickens.

    abstract::To develop an effective, safe, and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI) H5N1, we have constructed a recombinant Bacillus subtilis strain (B.S.-HA) expressing the hemagglutinin (HA) protein. Then we evaluated the immune function in chicken bone marrow derived dendritic cells...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38403

    authors: Mou C,Zhu L,Yang J,Xu W,Cheng X,Yang Q

    更新日期:2016-12-16 00:00:00

  • The psychometric properties and temporal dynamics of subjective stress, retrospectively assessed by different informants and questionnaires, and hair cortisol concentrations.

    abstract::To date, there is only scarce evidence for a considerable association of subjective and objective stress measures, which might be attributable to method bias (e.g., confounding) and/or asynchrony of their temporal changes. To validate different subjective stress measures by a physiological measure of long-term stress ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37526-2

    authors: Weckesser LJ,Dietz F,Schmidt K,Grass J,Kirschbaum C,Miller R

    更新日期:2019-01-31 00:00:00

  • Quantifying nucleoporin stoichiometry inside single nuclear pore complexes in vivo.

    abstract::The nuclear pore complex (NPC) is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring-scaffold perforates the nuclear envelope and features a unique molecular machinery that regulates nucleocytoplasmic transport. NPCs are composed of ~30 different nucleoporins (Nups), averaged at 8, 16...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09372

    authors: Mi L,Goryaynov A,Lindquist A,Rexach M,Yang W

    更新日期:2015-03-23 00:00:00

  • Molecular basis of canalization in an ascidian species complex adapted to different thermal conditions.

    abstract::Canalization is a result of intrinsic developmental buffering that ensures phenotypic robustness under genetic variation and environmental perturbation. As a consequence, animal phenotypes are remarkably consistent within a species under a wide range of conditions, a property that seems contradictory to evolutionary c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16717

    authors: Sato A,Kawashima T,Fujie M,Hughes S,Satoh N,Shimeld SM

    更新日期:2015-11-18 00:00:00

  • Amygdala-centred functional connectivity affects daily cortisol concentrations: a putative link with anxiety.

    abstract::The amygdala plays a critical role in emotion. Its functional coupling with the hippocampus and ventromedial prefrontal cortex extending to a portion of the anterior cingulate cortex (ACC) is implicated in anxiogenesis and hypothalamic-pituitary-adrenal (HPA) system regulation. However, it remains unclear how amygdala...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08918-7

    authors: Hakamata Y,Komi S,Moriguchi Y,Izawa S,Motomura Y,Sato E,Mizukami S,Kim Y,Hanakawa T,Inoue Y,Tagaya H

    更新日期:2017-08-16 00:00:00

  • Hypocretin in median raphe nucleus modulates footshock stimuli-induced REM sleep alteration.

    abstract::Stress is one of major factors that cause sleep problems. Hypocretin represents a stress-related neuropeptide and is well known in maintaining physiological wakefulness. The hypocretinergic neurons originate in the lateral hypothalamic area (LHA) and transmit to several brain regions, including the median raphe nuclei...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44731-0

    authors: Hsiao YT,Lo Y,Yi PL,Chang FC

    更新日期:2019-06-03 00:00:00

  • Low Cost Inkjet Printed Smart Bandage for Wireless Monitoring of Chronic Wounds.

    abstract::Chronic wounds affect millions of patients around the world and their treatment is challenging as the early signs indicating their development are subtle. In addition, a type of chronic wound, known as pressure ulcer, develops in patients with limited mobility. Infection and frequent bleeding are indicators of chronic...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28949

    authors: Farooqui MF,Shamim A

    更新日期:2016-06-29 00:00:00

  • Rescuing neuronal cell death by RAIDD- and PIDD- derived peptides and its implications for therapeutic intervention in neurodegenerative diseases.

    abstract::Caspase-2 is known to be involved in oxidative-stress mediated neuronal cell death. In this study, we demonstrated that rotenone-induced neuronal cell death is mediated by caspase-2 activation via PIDDosome formation. Our newly designed TAT-fused peptides, which contains wild-type helix number3 (H3) from RAIDD and PID...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31198

    authors: Jang TH,Lim IH,Kim CM,Choi JY,Kim EA,Lee TJ,Park HH

    更新日期:2016-08-09 00:00:00