Constitutive Differential Features of Type 2 Transglutaminase in Cells Derived from Celiac Patients and from Healthy Subjects.

Abstract:

:Type 2 transglutaminase (TG2) is a ubiquitous enzyme able to modify gliadin peptides introduced into the organism through the diet. By means of its catalytic activity, TG2 seems to have an important pathogenetic role in celiac disease (CD), an inflammatory intestinal disease caused by the ingestion of gluten-containing cereals. A strong autoimmune response to TG2 characterizes CD development. Anti-TG2 antibodies specifically derange the uptake of the α-gliadin peptide 31-43 by control, but not by celiac dermal fibroblasts, underlying some different constitutive features regarding TG2 in healthy and celiac subjects. Our aim was to investigate whether these differences depended on a different TG2 subcellular distribution and whether peptide 31-43 differentially regulated TG2 expression and activity in cells of the two groups of subjects. We found that TG2 was more abundantly associated with membranes of celiac fibroblasts than of control cells, in particular with the early endosomal and autophagic compartments. We also found that peptide 31-43 differentially affected TG2 expression and activity in the two groups of cells, activating TG2 more in control than in celiac cells and inducing TG2 expression in celiac cells, but not in control ones. The different TG2 subcellular localization and the different way the peptide 31-43 modulates TG2 activity and availability into control and CD cells suggested that TG2 is involved in the definition of a constitutive CD cellular phenotype, thus having an important and still undefined role in CD pathogenesis.

journal_name

Int J Mol Sci

authors

Paolella G,Nanayakkara M,Sposito S,Lepretti M,Auricchio S,Esposito C,Barone MV,Martucciello S,Caputo I

doi

10.3390/ijms21041231

subject

Has Abstract

pub_date

2020-02-12 00:00:00

issue

4

issn

1422-0067

pii

ijms21041231

journal_volume

21

pub_type

杂志文章
  • Ergosterol Peroxide from the Medicinal Mushroom Ganoderma lucidum Inhibits Differentiation and Lipid Accumulation of 3T3-L1 Adipocytes.

    abstract::Ergosterol peroxide is a natural compound of the steroid family found in many fungi, and it possesses antioxidant, anti-inflammatory, anticancer and antiviral activities. The anti-obesity activity of several edible and medicinal mushrooms has been reported, but the effect of mushroom-derived ergosterol peroxide on obe...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21020460

    authors: Jeong YU,Park YJ

    更新日期:2020-01-10 00:00:00

  • The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.

    abstract::The current interest of the scientific community for research in the field of calcium sensing in general and on the calcium-sensing Receptor (CaR) in particular is demonstrated by the still increasing number of papers published on this topic. The extracellular calcium-sensing receptor is the best-known G-protein-coupl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19040999

    authors: Gerbino A,Colella M

    更新日期:2018-03-27 00:00:00

  • Dual-Located WHIRLY1 Interacting with LHCA1 Alters Photochemical Activities of Photosystem I and Is Involved in Light Adaptation in Arabidopsis.

    abstract::Plastid-nucleus-located WHIRLY1 protein plays a role in regulating leaf senescence and is believed to associate with the increase of reactive oxygen species delivered from redox state of the photosynthetic electron transport chain. In order to make sure whether WHIRLY1 plays a role in photosynthesis, in this study, th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18112352

    authors: Huang D,Lin W,Deng B,Ren Y,Miao Y

    更新日期:2017-11-07 00:00:00

  • Intraductal Pancreatic Mucinous Neoplasms: A Tumor-Biology Based Approach for Risk Stratification.

    abstract::Pancreatic ductal adenocarcinoma is one of the most lethal human cancers. Its precursor lesions include pancreatic intra-epithelial neoplasia, mucinous cystic neoplasm, and intraductal papillary mucinous neoplasm (IPMN). IPMNs usually present as an incidental finding at imaging in 2.6% of the population and, according...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21176386

    authors: Nasca V,Chiaravalli M,Piro G,Esposito A,Salvatore L,Tortora G,Corbo V,Carbone C

    更新日期:2020-09-02 00:00:00

  • Methodology of Drought Stress Research: Experimental Setup and Physiological Characterization.

    abstract::Drought is one of the major stress factors affecting the growth and development of plants. In this context, drought-related losses of crop plant productivity impede sustainable agriculture all over the world. In general, plants respond to water deficits by multiple physiological and metabolic adaptations at the molecu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19124089

    authors: Osmolovskaya N,Shumilina J,Kim A,Didio A,Grishina T,Bilova T,Keltsieva OA,Zhukov V,Tikhonovich I,Tarakhovskaya E,Frolov A,Wessjohann LA

    更新日期:2018-12-17 00:00:00

  • G Protein-Coupled Receptors in Cancer.

    abstract::Despite the fact that G protein-coupled receptors (GPCRs) are the largest signal-conveying receptor family and mediate many physiological processes, their role in tumor biology is underappreciated. Numerous lines of evidence now associate GPCRs and their downstream signaling targets in cancer growth and development. I...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17081320

    authors: Bar-Shavit R,Maoz M,Kancharla A,Nag JK,Agranovich D,Grisaru-Granovsky S,Uziely B

    更新日期:2016-08-12 00:00:00

  • Natural Health Products (NHP's) and Natural Compounds as Therapeutic Agents for the Treatment of Cancer; Mechanisms of Anti-Cancer Activity of Natural Compounds and Overall Trends.

    abstract::Most cancer therapeutics, such as tubulin-targeting chemotherapy drugs, cause cytotoxic, non-selective effects. These harmful side-effects drastically reduce the cancer patient's quality of life. Recently, researchers have focused their efforts on studying natural health products (NHP's) which have demonstrated the ab...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21228480

    authors: Scaria B,Sood S,Raad C,Khanafer J,Jayachandiran R,Pupulin A,Grewal S,Okoko M,Arora M,Miles L,Pandey S

    更新日期:2020-11-11 00:00:00

  • The Role of Peroxisome Proliferator-Activated Receptors and Their Transcriptional Coactivators Gene Variations in Human Trainability: A Systematic Review.

    abstract:BACKGROUND:The peroxisome proliferator-activated receptors (PPARA, PPARG, PPARD) and their transcriptional coactivators' (PPARGC1A, PPARGC1B) gene polymorphisms have been associated with muscle morphology, oxygen uptake, power output and endurance performance. The purpose of this review is to determine whether the PPAR...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,meta分析,评审

    doi:10.3390/ijms19051472

    authors: Petr M,Stastny P,Zajac A,Tufano JJ,Maciejewska-Skrendo A

    更新日期:2018-05-15 00:00:00

  • Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells.

    abstract::Human induced pluripotent stem cells (iPSCs) hold enormous promise for regenerative medicine. The major safety concern is the tumorigenicity of transplanted cells derived from iPSCs. A potential solution would be to introduce a suicide gene into iPSCs as a safety switch. The herpes simplex virus type 1 thymidine kinas...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20040810

    authors: Iwasawa C,Tamura R,Sugiura Y,Suzuki S,Kuzumaki N,Narita M,Suematsu M,Nakamura M,Yoshida K,Toda M,Okano H,Miyoshi H

    更新日期:2019-02-14 00:00:00

  • Novel Selective Estrogen Receptor Modulator Ameliorates Murine Colitis.

    abstract::Estrogen-receptor-mediated signaling has been suggested to decrease the inflammatory response in monocyte macrophages. Previously, we showed that a novel selective estrogen receptor modulator (SERM2) promotes anti-inflammatory phenotype of monocytes in vitro. In this study, we demonstrate the potential of SERM2 in ame...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20123007

    authors: Polari L,Anttila S,Helenius T,Wiklund A,Linnanen T,Toivola DM,Määttä J

    更新日期:2019-06-20 00:00:00

  • Modulation of Innate Immunity by lignin-Carbohydrate, a Novel TLR4 Ligand, Results in Augmentation of Mucosal IgA and Systemic IgG Production.

    abstract::Previous study revealed that a specific lignin-carbohydrate preparation, named as lignin-rich enzyme lignin (LREL) derived from plant husk, is a novel toll-like receptor 4 ligand and shows a potent immune-stimulatory activity against dendritic cells (DCs) in vitro. In this report, we investigated immune-stimulatory ac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19010064

    authors: Tsuji R,Ikado K,Fujiwara D

    更新日期:2017-12-26 00:00:00

  • Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.

    abstract::Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the struct...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19102912

    authors: Rijal G,Wang J,Yu I,Gang DR,Chen RK,Li W

    更新日期:2018-09-25 00:00:00

  • Salivary MicroRNA for Diagnosis of Cancer and Systemic Diseases: A Systematic Review.

    abstract::: Background: The aberrant expression of microRNAs (miRNAs) has been associated with several diseases, including cancer, inflammatory, and autoimmune conditions. Interest in salivary miRNAs as non-invasive tools for the diagnosis of malignancies and systemic diseases is rapidly increasing. The present systematic revie...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21030907

    authors: Setti G,Pezzi ME,Viani MV,Pertinhez TA,Cassi D,Magnoni C,Bellini P,Musolino A,Vescovi P,Meleti M

    更新日期:2020-01-30 00:00:00

  • The Potential Roles of the Apoptosis-Related Protein PDRG1 in Diapause Embryo Restarting of Artemia sinica.

    abstract::High salinity and low temperatures can induce Artemia sinica to enter the diapause stage during embryonic development. Diapause embryos stop at the gastrula stage, allowing them to resist apoptosis and regulate cell cycle activity to guarantee normal development after diapause termination. P53 and DNA damage-regulated...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19010126

    authors: Zhang W,Yao F,Zhang H,Li N,Zou X,Sui L,Hou L

    更新日期:2018-01-02 00:00:00

  • Nemo-Like Kinase in Development and Diseases: Insights from Mouse Studies.

    abstract::The Wnt signalling pathway is a central communication cascade between cells to orchestrate polarity and fate during development and adult tissue homeostasis in various organisms. This pathway can be regulated by different signalling molecules in several steps. One of the coordinators in this pathway is Nemo-like kinas...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21239203

    authors: Daams R,Massoumi R

    更新日期:2020-12-02 00:00:00

  • Antioxidant-induced stress.

    abstract::Antioxidants are among the most popular health-protecting products, sold worldwide without prescription. Indeed, there are many reports showing the benefits of antioxidants but only a few questioning the possible harmful effects of these "drugs". The normal balance between antioxidants and free radicals in the body is...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms13022091

    authors: Villanueva C,Kross RD

    更新日期:2012-01-01 00:00:00

  • Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications.

    abstract::Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21207730

    authors: Qiu C,Ge Z,Cui W,Yu L,Li J

    更新日期:2020-10-19 00:00:00

  • Non-Native Conformational Isomers of the Catalytic Domain of PCSK9 Induce an Immune Response, Reduce Lipids and Increase LDL Receptor Levels.

    abstract::PCSK9 (Proprotein convertase subtilisin/kexin type 9) increases plasma cholesterol levels by promoting LDL receptor degradation. Current antibody inhibitors block the interaction between PCSK9 and LDL receptors, significantly decrease plasma cholesterol levels, and provide beneficial clinical outcomes. To reduce the a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19020640

    authors: Jiang C,Nischal H,Sun H,Li L,Cao Y,Wei P,Chang JY,Teng BB

    更新日期:2018-02-24 00:00:00

  • Hepatitis C Virus and Hepatocellular Carcinoma: When the Host Loses Its Grip.

    abstract::Chronic infection with hepatitis C virus (HCV) is a major cause of hepatocellular carcinoma (HCC). Novel treatments with direct-acting antivirals achieve high rates of sustained virologic response; however, the HCC risk remains elevated in cured patients, especially those with advanced liver disease. Long-term HCV inf...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21093057

    authors: Goto K,Roca Suarez AA,Wrensch F,Baumert TF,Lupberger J

    更新日期:2020-04-26 00:00:00

  • Potent Anti-Cancer Properties of Phthalimide-Based Curcumin Derivatives on Prostate Tumor Cells.

    abstract::Metastatic castration-resistant prostate cancer is commonly treated with chemotherapy, whose effect is less than satisfactory. This raised the need for novel agents for the treatment of prostate cancer. In the present study, five phthalimide-based curcumin derivatives were synthesized and completely characterized to a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20010028

    authors: Belluti S,Orteca G,Semeghini V,Rigillo G,Parenti F,Ferrari E,Imbriano C

    更新日期:2018-12-21 00:00:00

  • Structural Diversity and Highly Specific Host-Pathogen Transcriptional Regulation of Defensin Genes Is Revealed in Tomato.

    abstract::Defensins are small and rather ubiquitous cysteine-rich anti-microbial peptides. These proteins may act against pathogenic microorganisms either directly (by binding and disrupting membranes) or indirectly (as signaling molecules that participate in the organization of the cellular defense). Even though defensins are ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21249380

    authors: Nikoloudakis N,Pappi P,Markakis EA,Charova SN,Fanourakis D,Paschalidis K,Delis C,Tzortzakakis EA,Tsaniklidis G

    更新日期:2020-12-09 00:00:00

  • Synthesis and Evaluation of Neuroprotective Selenoflavanones.

    abstract::The physicochemical properties and antioxidant activity of a molecule could be improved by the substitution of an oxygen atom in a molecule with selenium. We synthesized selenoflavanones and flavanones to evaluate their neuroprotective effects. The selenoflavanones showed improved physicochemical properties, suggestiv...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms161226188

    authors: Choi YS,Kim DM,Kim YJ,Yang S,Lee KT,Ryu JH,Jeong JH

    更新日期:2015-12-10 00:00:00

  • The Impact of O-Glycosylation on Cyanidin Interaction with RBCs and HMEC-1 Cells-Structure⁻Activity Relationships.

    abstract::With the aim of contributing to the knowledge about their potential therapeutic activity, we determined the biological activities of cyanidin and its selected O-glycosides in relation to erythrocytes (RBCs) and human dermal vascular endothelial cells (HMEC-1). Furthermore, on the basis of changes in the physical/funct...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20081928

    authors: Cyboran-Mikołajczyk S,Solarska-Ściuk K,Mieszała K,Glatzel-Plucińska N,Matczak K,Kleszczyńska H

    更新日期:2019-04-19 00:00:00

  • Maternal Tryptophan Supplementation Protects Adult Rat Offspring against Hypertension Programmed by Maternal Chronic Kidney Disease: Implication of Tryptophan-Metabolizing Microbiome and Aryl Hydrocarbon Receptor.

    abstract::Hypertension and chronic kidney disease (CKD) can originate during early-life. Tryptophan metabolites generated by different pathways have both detrimental and beneficial effects. In CKD, uremic toxins from the tryptophan-generating metabolites are endogenous ligands of the aryl hydrocarbon receptor (AHR). The interpl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21124552

    authors: Hsu CN,Lin IC,Yu HR,Huang LT,Tiao MM,Tain YL

    更新日期:2020-06-26 00:00:00

  • Collateral Vessels Have Unique Endothelial and Smooth Muscle Cell Phenotypes.

    abstract::Collaterals are unique blood vessels present in the microcirculation of most tissues that, by cross-connecting a small fraction of the outer branches of adjacent arterial trees, provide alternate routes of perfusion. However, collaterals are especially susceptible to rarefaction caused by aging, other vascular risk fa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20153608

    authors: Zhang H,Chalothorn D,Faber JE

    更新日期:2019-07-24 00:00:00

  • Platelet-Rich Plasma Promotes the Proliferation of Human Keratinocytes via a Progression of the Cell Cycle. A Role of Prolidase.

    abstract::Although the role of platelet-rich plasma (PRP) in tissue regeneration has been confirmed in many studies, the mechanism of this process is still not fully understood. Human keratinocytes (HaCaT) cells were used as an experimental model for studies on the effects of PRP on cell proliferation, migration, collagen biosy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms22020936

    authors: Misiura M,Guszczyn T,Oscilowska I,Baszanowska W,Palka J,Miltyk W

    更新日期:2021-01-19 00:00:00

  • Overcoming the recalcitrance for the conversion of kenaf pulp to glucose via microwave-assisted pre-treatment processes.

    abstract::This study evaluates the pre-treatment of cellulose from kenaf plant to yield sugar precursors for the production of ethanol or butanol for use as biofuel additives. In order to convert the crystalline cellulosic form to the amorphous form that can undergo enzymatic hydrolysis of the glycosidic bond to yield sugars, k...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms12031451

    authors: Ooi BG,Rambo AL,Hurtado MA

    更新日期:2011-01-01 00:00:00

  • Combined 3D-QSAR modeling and molecular docking studies on pyrrole-indolin-2-ones as Aurora A kinase inhibitors.

    abstract::Aurora kinases have emerged as attractive targets for the design of anticancer drugs. 3D-QSAR (comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA)) and Surflex-docking studies were performed on a series of pyrrole-indoline-2-ones as Aurora A inhibitors. The CoMFA...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms12031605

    authors: Ai Y,Wang ST,Sun PH,Song FJ

    更新日期:2011-01-01 00:00:00

  • Buprofezin Is Metabolized by CYP353D1v2, a Cytochrome P450 Associated with Imidacloprid Resistance in Laodelphax striatellus.

    abstract::CYP353D1v2 is a cytochrome P450 related to imidacloprid resistance in Laodelphax striatellus. This work was conducted to examine the ability of CYP353D1v2 to metabolize other insecticides. Carbon monoxide difference spectra analysis indicates that CYP353D1v2 was successfully expressed in insect cell Sf9. The catalytic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms18122564

    authors: Elzaki MEA,Miah MA,Han Z

    更新日期:2017-11-29 00:00:00

  • Current Understanding of Residual Force Enhancement: Cross-Bridge Component and Non-Cross-Bridge Component.

    abstract::Muscle contraction is initiated by the interaction between actin and myosin filaments. The sliding of actin filaments relative to myosin filaments is produced by cross-bridge cycling, which is governed by the theoretical framework of the cross-bridge theory. The cross-bridge theory explains well a number of mechanical...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20215479

    authors: Fukutani A,Herzog W

    更新日期:2019-11-04 00:00:00