Abstract:
:Spinal cord injury (SCI) triggers pronounced inflammatory responses that are accompanied by neuronal disruption and functional deficits. SCI treatment remains an unmet clinical need. Emerging evidence suggests that riluzole may exert a neuroprotective effect due to its anti-inflammatory properties. However, details of the underlying mechanisms remain poorly defined. The polarization of microglial/macrophages has an important role in neuroinflammation. Here, we examined whether riluzole can exert a neuroprotective effect after acute SCI, and whether this effect is associated with changes in microglia/macrophages polarization. Riluzole (4 mg/kg) or vehicle were injected intraperitoneally (i.p.) in female rats immediately following SCI and repeated for 7 consecutive days (b.i.d.). Compared with vehicle treatment, riluzole-treated SCI rats showed significant higher locomotor scores (Basso, Beattie, and Bresnahan score, Inclined Plane test score, n = 18/group). Riluzole-treated rats also developed smaller spinal cavities, showed higher levels of myelin basic protein (MBP) and neurofilament (NF)200 immunoreactivities, and lower levels of proinflammatory cytokines in the spinal cord at 7 days post-SCI. Immunofluorescence study revealed more CD206+ cells and less iNOS+ cells in the injured spinal cord of riluzole-treated SCI rats, as compared to vehicle control. Using real-time PCR, we found that riluzole upregulated the mRNA levels of M2 markers, but downregulated that of M1 markers, as compared to the vehicle treatment. Current findings suggest that systemic administration of riluzole after acute SCI facilitated motor function recovery and inhibited inflammatory responses, which may be associated with polarization of M2 microglia/macrophages.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Wu Q,Zhang Y,Zhang Y,Zhang W,Zhang W,Liu Y,Xu S,Guan Y,Chen Xdoi
10.1016/j.neulet.2020.134829subject
Has Abstractpub_date
2020-04-01 00:00:00pages
134829eissn
0304-3940issn
1872-7972pii
S0304-3940(20)30099-9journal_volume
723pub_type
杂志文章abstract::The presence and micro-anatomical localization of muscarinic cholinergic receptors were assessed in dorsal root ganglia of chick embryo during development using radioligand binding and immunocytochemical techniques, respectively. The non-selective muscarinic cholinergic receptor radioligand [3H]quinuclidinyl benzilate...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11474-b
更新日期:1995-04-21 00:00:00
abstract::In order to study the rapidity of interaction between tetraethylammonium (TEA) and the delayed K+ channel of the mammalian brain neuron, TEA was applied to isolated rat hippocampal pyramidal neurons in a time-controlled sequence with a voltage step using a concentration clamp technique. TEA at a concentration of 30 mM...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(91)90601-o
更新日期:1991-10-14 00:00:00
abstract::An in vitro assay was used to determine the effects of conditioning nerve lesions on the regeneration of adult rat retinal ganglion cell (RGC) axons from retinal explants. Following the conditioning lesion (CL) of unilateral optic nerve transection, maximal regrowth was seen from RGC explanted from ipsilateral retinae...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90140-7
更新日期:1989-02-13 00:00:00
abstract::Inhibition of return (IOR) refers to the slowing of responses to a target that appears in the same location as a previous event. Many researchers have speculated that IOR arises from inhibitory neural processes that have developed through evolution to facilitate efficient search patterns by biasing the action and/or a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.05.013
更新日期:2005-09-09 00:00:00
abstract::Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody prepar...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.06.006
更新日期:2006-09-01 00:00:00
abstract::Anterograde degeneration methods demonstrated two efferent components from the trigeminal principal sensory nucleus (PrV) to the thalamic ventroposteromedial nucleus (VPM) in the squirrel monkey: fibers from the dorsal PrV coursed within the central tegmental tract and terminated in a dorsoventromedial strip of the ip...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90138-2
更新日期:1986-07-24 00:00:00
abstract::The combination of ethanol and cocaine is commonly abused by human addicts which has serious clinical consequences. Here, the effects of separately and concurrently administered ethanol and 'binge' cocaine on kappa opioid receptor (KOR) mRNA in the ventral tegmental area (VTA) and nucleus accumbens (NAc) of rats were ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00675-8
更新日期:1999-11-05 00:00:00
abstract::Ts65Dn (TS), control littermates (CO) and Swiss (SW) male mice were tested in the elevated plus-maze and in the Morris water maze (MWM) for memory evaluation. In the plus-maze, each mouse was placed at the end of an open arm and initial freezing and the time to enter into an enclosed arm (transfer latency) were measur...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00317-6
更新日期:1998-05-15 00:00:00
abstract::Interleukin-1beta (IL1beta) and tumor necrosis factor-alpha (TNFalpha) are involved in several physiological functions regulated by brain. The current studies were performed to determine whether a diurnal rhythm of IL1beta and TNFalpha mRNAs, determined by real time RT-PCR, existed in specific brain regions linked to ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.07.019
更新日期:2003-11-27 00:00:00
abstract::The pharmacological properties of presynaptic calcium (Ca) channels on rat hippocampal mossy fiber synaptosomes were characterized by determining the inhibitory potencies for various classes of Ca antagonists on depolarization-induced Ca mobilization and the release of dynorphin A(1-8)-like immunoreactivity (Dyn-LI). ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90184-5
更新日期:1989-12-04 00:00:00
abstract::Mitogen-activated protein kinases (MAPK) can be activated by opioids such as morphine via opioid receptor, and their activations have been observed in synaptic plasticity, learning, memory and addiction. Long-term exposure to morphine may induce physical dependence, manifested as somatic withdrawal symptoms such as di...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.11.030
更新日期:2010-01-14 00:00:00
abstract::Freezing is one of the most widely recognized defensive reactions to approaching threats in animals. Here we tested whether the same stimuli can elicit freeze-like responses in healthy humans as well. We used a modified version of the two-frame apparent motion paradigm, in which both size and location of a stimulus wi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.12.038
更新日期:2014-02-21 00:00:00
abstract::Schisandra chinensis is commonly used for food and as a traditional remedy for the treatment of neuronal disorders. However, it is unclear which component of S. chinensis is responsible for its neuropharmacological effects. To answer this question, we isolated α-iso-cubebene, a dibenzocyclooctadiene lignin, from S. ch...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.09.053
更新日期:2013-10-25 00:00:00
abstract::Chemical irritation of the urinary bladder with formalin in the rat induced c-fos protein-like immunoreactivity in more than 80% of substance P receptor-like immunoreactive (SPR-LI) neurons of the dorsal commissural nucleus, sacral parasympathetic nucleus and lamina I in the 6th lumbar and 1st sacral cord segments. Th...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(95)11991-5
更新日期:1995-09-29 00:00:00
abstract::The human rhabdomyosarcoma cell line TE671 expresses the foetal subtype of muscle acetylcholine receptor (AChR). By transfecting TE671 cells with cDNA encoding the human muscle AChR epsilon subunit under the control of the cytomegalovirus promoter we have established a stable cell clone that, in addition, constitutive...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12488-5
更新日期:1996-03-22 00:00:00
abstract::Recently, Monfils et al. [9] and Clem and Huganir [3] have shown that an isolated retrieval trial before the extinction sessions (retrieval-extinction) in mice and rats prevented the renewal and spontaneous recovery of the original fear memory by inhibiting reconsolidation in a hippocampus-independent manner. In contr...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2012.06.048
更新日期:2012-08-08 00:00:00
abstract::Four substance P (SP) antagonists were tested on anaesthetized rats, by injecting 8 microgram amounts into the spinal cord (T8-T9), and by observing their effects on the hypothalamo-neurohypophysial responses to a presumably painful stimulus, the superfusion of the hepatic portal vein with 1 microM bradykinin. Only tw...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(83)90444-5
更新日期:1983-06-30 00:00:00
abstract::We studied the number of large-sized neurons and neurofibrillary tangles (NFT) in the tuberomammillary nucleus (TM) of the hypothalamus from cases with Alzheimer's disease (AD) and age-matched controls. Numerous NFT were found in TM of AD. However, NFT was never observed in this nucleus of age-matched controls. The nu...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(93)90019-h
更新日期:1993-03-19 00:00:00
abstract::The kynurenine pathway converts tryptophan into various compounds, including L-kynurenine, which in turn can be converted to the excitatory amino acid receptor antagonist kynurenic acid. The hypothesis that endogenously-produced kynurenic acid could have physiological effects was tested in combined entorhinal/hippocam...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)13472-3
更新日期:1997-03-14 00:00:00
abstract::Stress increases associative learning and the density of dendritic spines in the hippocampus of male rats. In contrast, exposure to the same stressor impairs associative learning and reduces spine density in females. These effects in females are most evident when they are in the proestrus phase of the estrous cycle. A...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.10.051
更新日期:2009-01-02 00:00:00
abstract::The distribution of substance P (SP)-like immunoreactivity in the rat retina was investigated by immunohistochemistry. SP-positive cells were found throughout the retina. The majority of them were located in the proximal portion of the inner nuclear layer and the processes from these cells directed to the inner plexif...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90004-5
更新日期:1981-05-29 00:00:00
abstract::The ubiquitin carboxy-terminal hydrolase L1gene (UCH-L1) has been implicated in the aetiology of Parkinson's disease (PD). A rare Ile93Met mutation in UCH-L1 in a German PD sib-pair has been reported. Recently, a S18Y (C54A) polymorphism in exon 3 of UCH-L1 was found to be under-represented in PD patients compared to ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01510-x
更新日期:2000-10-27 00:00:00
abstract::Oxygen tension is critical for proliferation of human and murine midbrain-derived neural precursor cells (mNPCs). Lack of hypoxia-inducible factor-1α (HIF1α) impairs midbrain dopaminergic neurogenesis which could be rescued by vascular endothelial growth factor (VEGF) via VEGFR-2 signaling. Here, we conditionally inac...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2013.08.031
更新日期:2013-10-11 00:00:00
abstract::In vivo rates of incorporation and turnover of palmitate and arachidonate in brain phospholipids were measured in awake rats treated chronically with lithium, following intravenous infusion of radiolabeled palmitate and arachidonate, respectively. Chronic lithium, at a brain level considered to be therapeutic in human...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)13264-x
更新日期:1996-12-20 00:00:00
abstract::Chronic stress is well known to exacerbate pain. We tested the hypothesis that neonatal handling, which induces resilience to the negative impact of stress by increasing the quality and quantity of maternal care, attenuates the mechanical hyperalgesia produced by water-avoidance stress in the adult rat. Neonatal male ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.01.066
更新日期:2015-03-30 00:00:00
abstract::The survival of developing sensory neurons is dependent upon target-derived growth factors, in their absence neurons undergo programmed cell death. The molecular mechanisms underpinning neuronal cell survival and death are poorly understood. Tyrosine kinases are important signalling proteins that have been implicated ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00318-2
更新日期:1997-05-16 00:00:00
abstract::The effect of the spectral tone structure on pre-attentive and attentive pitch discrimination was investigated. The mismatch negativity (MMN) component was recorded from reading subjects to pitch changes of identical magnitude in pure tones with only one sinusoidal frequency component and in spectrally rich tones with...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00941-6
更新日期:2000-01-21 00:00:00
abstract::Tinnitus is the perception of sound in the absence of any external auditory stimulus. Based on previous research we have proposed a framework which postulates that the reduction of ongoing inhibitory alpha activity in tinnitus subjects favors a synchronization of neurons in the gamma frequency range while in a resting...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.02.028
更新日期:2009-04-10 00:00:00
abstract::Using double-staining techniques, the distribution of NADPH-diaphorase (ND)- and nitric oxide synthase (NOS)-positive cells was compared in the periglomerular region of typical and atypical rat olfactory glomeruli. Dorsomedial and ventrolateral atypical glomeruli contained similar number of ND/NOS-positive periglomeru...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(96)12424-1
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND:Diverse physiological and pathological functions of 27-hydroxycholesterol (27-OHC) were proved. However, cytotoxicity and potential influence of 27-OHC on cholesterol metabolism in neurons are unclear. DESIGN AND METHODS:In the vitro co-culture system, SH-SY5Y cells and C6 cells were applied to explore the ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.08.056
更新日期:2016-10-06 00:00:00