Riluzole improves functional recovery after acute spinal cord injury in rats and may be associated with changes in spinal microglia/macrophages polarization.


:Spinal cord injury (SCI) triggers pronounced inflammatory responses that are accompanied by neuronal disruption and functional deficits. SCI treatment remains an unmet clinical need. Emerging evidence suggests that riluzole may exert a neuroprotective effect due to its anti-inflammatory properties. However, details of the underlying mechanisms remain poorly defined. The polarization of microglial/macrophages has an important role in neuroinflammation. Here, we examined whether riluzole can exert a neuroprotective effect after acute SCI, and whether this effect is associated with changes in microglia/macrophages polarization. Riluzole (4 mg/kg) or vehicle were injected intraperitoneally (i.p.) in female rats immediately following SCI and repeated for 7 consecutive days (b.i.d.). Compared with vehicle treatment, riluzole-treated SCI rats showed significant higher locomotor scores (Basso, Beattie, and Bresnahan score, Inclined Plane test score, n = 18/group). Riluzole-treated rats also developed smaller spinal cavities, showed higher levels of myelin basic protein (MBP) and neurofilament (NF)200 immunoreactivities, and lower levels of proinflammatory cytokines in the spinal cord at 7 days post-SCI. Immunofluorescence study revealed more CD206+ cells and less iNOS+ cells in the injured spinal cord of riluzole-treated SCI rats, as compared to vehicle control. Using real-time PCR, we found that riluzole upregulated the mRNA levels of M2 markers, but downregulated that of M1 markers, as compared to the vehicle treatment. Current findings suggest that systemic administration of riluzole after acute SCI facilitated motor function recovery and inhibited inflammatory responses, which may be associated with polarization of M2 microglia/macrophages.


Neurosci Lett


Neuroscience letters


Wu Q,Zhang Y,Zhang Y,Zhang W,Zhang W,Liu Y,Xu S,Guan Y,Chen X




Has Abstract


2020-04-01 00:00:00












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    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Tata AM,Biagioni S,Ricci A,Amenta F,Augusti-Tocco G

    更新日期:1995-04-21 00:00:00

  • Rapid interaction of tetraethylammonium with delayed K+ channel in mammalian brain neuron.

    abstract::In order to study the rapidity of interaction between tetraethylammonium (TEA) and the delayed K+ channel of the mammalian brain neuron, TEA was applied to isolated rat hippocampal pyramidal neurons in a time-controlled sequence with a voltage step using a concentration clamp technique. TEA at a concentration of 30 mM...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Oyama Y,Harata N,Akaike N

    更新日期:1991-10-14 00:00:00

  • The conditioning effect of optic nerve injury upon axonal regrowth from adult rat retinal ganglion cells explanted in vitro.

    abstract::An in vitro assay was used to determine the effects of conditioning nerve lesions on the regeneration of adult rat retinal ganglion cell (RGC) axons from retinal explants. Following the conditioning lesion (CL) of unilateral optic nerve transection, maximal regrowth was seen from RGC explanted from ipsilateral retinae...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Johnson AR,Gregson NA,Wigley CB,Berry M

    更新日期:1989-02-13 00:00:00

  • Does Joe influence Fred's action? Inhibition of return across different nervous systems.

    abstract::Inhibition of return (IOR) refers to the slowing of responses to a target that appears in the same location as a previous event. Many researchers have speculated that IOR arises from inhibitory neural processes that have developed through evolution to facilitate efficient search patterns by biasing the action and/or a...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Welsh TN,Elliott D,Anson JG,Dhillon V,Weeks DJ,Lyons JL,Chua R

    更新日期:2005-09-09 00:00:00

  • Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells.

    abstract::Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody prepar...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Kutty RK,Samuel W,Chen S,Vijayasarathy C,Dun Y,Mysona B,Wiggert B,Smith SB

    更新日期:2006-09-01 00:00:00

  • Dual efferent projections of the trigeminal principal sensory nucleus to the thalamic ventroposteromedial nucleus in the squirrel monkey.

    abstract::Anterograde degeneration methods demonstrated two efferent components from the trigeminal principal sensory nucleus (PrV) to the thalamic ventroposteromedial nucleus (VPM) in the squirrel monkey: fibers from the dorsal PrV coursed within the central tegmental tract and terminated in a dorsoventromedial strip of the ip...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Ganchrow D,Mehler WR

    更新日期:1986-07-24 00:00:00

  • Downregulation of kappa opioid receptor mRNA levels by chronic ethanol and repetitive cocaine in rat ventral tegmentum and nucleus accumbens.

    abstract::The combination of ethanol and cocaine is commonly abused by human addicts which has serious clinical consequences. Here, the effects of separately and concurrently administered ethanol and 'binge' cocaine on kappa opioid receptor (KOR) mRNA in the ventral tegmental area (VTA) and nucleus accumbens (NAc) of rats were ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Rosin A,Lindholm S,Franck J,Georgieva J

    更新日期:1999-11-05 00:00:00

  • Impaired short- and long-term memory in Ts65Dn mice, a model for Down syndrome.

    abstract::Ts65Dn (TS), control littermates (CO) and Swiss (SW) male mice were tested in the elevated plus-maze and in the Morris water maze (MWM) for memory evaluation. In the plus-maze, each mouse was placed at the end of an open arm and initial freezing and the time to enter into an enclosed arm (transfer latency) were measur...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Escorihuela RM,Vallina IF,Martínez-Cué C,Baamonde C,Dierssen M,Tobeña A,Flórez J,Fernández-Teruel A

    更新日期:1998-05-15 00:00:00

  • Time of day differences in IL1beta and TNFalpha mRNA levels in specific regions of the rat brain.

    abstract::Interleukin-1beta (IL1beta) and tumor necrosis factor-alpha (TNFalpha) are involved in several physiological functions regulated by brain. The current studies were performed to determine whether a diurnal rhythm of IL1beta and TNFalpha mRNAs, determined by real time RT-PCR, existed in specific brain regions linked to ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Cearley C,Churchill L,Krueger JM

    更新日期:2003-11-27 00:00:00

  • Effects of calcium antagonists on the evoked release of dynorphin A(1-8) and availability of intraterminal calcium in rat hippocampal mossy fiber synaptosomes.

    abstract::The pharmacological properties of presynaptic calcium (Ca) channels on rat hippocampal mossy fiber synaptosomes were characterized by determining the inhibitory potencies for various classes of Ca antagonists on depolarization-induced Ca mobilization and the release of dynorphin A(1-8)-like immunoreactivity (Dyn-LI). ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Terrian DM,Damron DS,Dorman RV,Gannon RL

    更新日期:1989-12-04 00:00:00

  • Naloxone-precipitated withdrawal enhances ERK phosphorylation in prefrontal association cortex and accumbens nucleus of morphine-dependent mice.

    abstract::Mitogen-activated protein kinases (MAPK) can be activated by opioids such as morphine via opioid receptor, and their activations have been observed in synaptic plasticity, learning, memory and addiction. Long-term exposure to morphine may induce physical dependence, manifested as somatic withdrawal symptoms such as di...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Li T,Hou Y,Cao W,Yan CX,Chen T,Li SB

    更新日期:2010-01-14 00:00:00

  • Approaching threats elicit a freeze-like response in humans.

    abstract::Freezing is one of the most widely recognized defensive reactions to approaching threats in animals. Here we tested whether the same stimuli can elicit freeze-like responses in healthy humans as well. We used a modified version of the two-frame apparent motion paradigm, in which both size and location of a stimulus wi...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Sagliano L,Cappuccio A,Trojano L,Conson M

    更新日期:2014-02-21 00:00:00

  • α-Iso-cubebene exerts neuroprotective effects in amyloid beta stimulated microglia activation.

    abstract::Schisandra chinensis is commonly used for food and as a traditional remedy for the treatment of neuronal disorders. However, it is unclear which component of S. chinensis is responsible for its neuropharmacological effects. To answer this question, we isolated α-iso-cubebene, a dibenzocyclooctadiene lignin, from S. ch...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Park SY,Park SJ,Park NJ,Joo WH,Lee SJ,Choi YW

    更新日期:2013-10-25 00:00:00

  • Expression of c-fos protein in substance P receptor-like immunoreactive neurons in response to noxious stimuli on the urinary bladder: an observation in the lumbosacral cord segments of the rat.

    abstract::Chemical irritation of the urinary bladder with formalin in the rat induced c-fos protein-like immunoreactivity in more than 80% of substance P receptor-like immunoreactive (SPR-LI) neurons of the dorsal commissural nucleus, sacral parasympathetic nucleus and lamina I in the 6th lumbar and 1st sacral cord segments. Th...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Lü Y,Jin SX,Xu TL,Qin BZ,Li JS,Ding YQ,Shigemoto R,Mizuno N

    更新日期:1995-09-29 00:00:00

  • Stable functional expression of the adult subtype of human muscle acetylcholine receptor following transfection of the human rhabdomyosarcoma cell line TE671 with cDNA encoding the epsilon subunit.

    abstract::The human rhabdomyosarcoma cell line TE671 expresses the foetal subtype of muscle acetylcholine receptor (AChR). By transfecting TE671 cells with cDNA encoding the human muscle AChR epsilon subunit under the control of the cytomegalovirus promoter we have established a stable cell clone that, in addition, constitutive...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Beeson D,Amar M,Bermudez I,Vincent A,Newsom-Davis J

    更新日期:1996-03-22 00:00:00

  • No erasure effect of retrieval-extinction trial on fear memory in the hippocampus-independent and dependent paradigms.

    abstract::Recently, Monfils et al. [9] and Clem and Huganir [3] have shown that an isolated retrieval trial before the extinction sessions (retrieval-extinction) in mice and rats prevented the renewal and spontaneous recovery of the original fear memory by inhibiting reconsolidation in a hippocampus-independent manner. In contr...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Ishii D,Matsuzawa D,Matsuda S,Tomizawa H,Sutoh C,Shimizu E

    更新日期:2012-08-08 00:00:00

  • Neural actions of several substance P antagonists in the rat spinal cord.

    abstract::Four substance P (SP) antagonists were tested on anaesthetized rats, by injecting 8 microgram amounts into the spinal cord (T8-T9), and by observing their effects on the hypothalamo-neurohypophysial responses to a presumably painful stimulus, the superfusion of the hepatic portal vein with 1 microM bradykinin. Only tw...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Stoppini L,Baertschi AJ,Mathison R,Barja F

    更新日期:1983-06-30 00:00:00

  • Loss of large neurons and occurrence of neurofibrillary tangles in the tuberomammillary nucleus of patients with Alzheimer's disease.

    abstract::We studied the number of large-sized neurons and neurofibrillary tangles (NFT) in the tuberomammillary nucleus (TM) of the hypothalamus from cases with Alzheimer's disease (AD) and age-matched controls. Numerous NFT were found in TM of AD. However, NFT was never observed in this nucleus of age-matched controls. The nu...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Nakamura S,Takemura M,Ohnishi K,Suenaga T,Nishimura M,Akiguchi I,Kimura J,Kimura T

    更新日期:1993-03-19 00:00:00

  • Pretreatment with L-kynurenine, the precursor to the excitatory amino acid antagonist kynurenic acid, suppresses epileptiform activity in combined entorhinal/hippocampal slices.

    abstract::The kynurenine pathway converts tryptophan into various compounds, including L-kynurenine, which in turn can be converted to the excitatory amino acid receptor antagonist kynurenic acid. The hypothesis that endogenously-produced kynurenic acid could have physiological effects was tested in combined entorhinal/hippocam...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Scharfman HE,Ofer A

    更新日期:1997-03-14 00:00:00

  • Stressful experience has opposite effects on dendritic spines in the hippocampus of cycling versus masculinized females.

    abstract::Stress increases associative learning and the density of dendritic spines in the hippocampus of male rats. In contrast, exposure to the same stressor impairs associative learning and reduces spine density in females. These effects in females are most evident when they are in the proestrus phase of the estrous cycle. A...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Dalla C,Whetstone AS,Hodes GE,Shors TJ

    更新日期:2009-01-02 00:00:00

  • Demonstration of a substance P-like immunoreactivity in retinal cells of the rat.

    abstract::The distribution of substance P (SP)-like immunoreactivity in the rat retina was investigated by immunohistochemistry. SP-positive cells were found throughout the retina. The majority of them were located in the proximal portion of the inner nuclear layer and the processes from these cells directed to the inner plexif...

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    pub_type: 杂志文章


    authors: Fukuda M,Kuwayama Y,Shiosaka S,Ishimoto I,Shimizu Y,Takagi H,Inagaki S,Sakanaka M,Semba E,Takatsuki K,Tohyama M

    更新日期:1981-05-29 00:00:00

  • The ubiquitin carboxy-terminal hydrolase-L1 gene S18Y polymorphism does not confer protection against idiopathic Parkinson's disease.

    abstract::The ubiquitin carboxy-terminal hydrolase L1gene (UCH-L1) has been implicated in the aetiology of Parkinson's disease (PD). A rare Ile93Met mutation in UCH-L1 in a German PD sib-pair has been reported. Recently, a S18Y (C54A) polymorphism in exon 3 of UCH-L1 was found to be under-represented in PD patients compared to ...

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    pub_type: 杂志文章


    authors: Mellick GD,Silburn PA

    更新日期:2000-10-27 00:00:00

  • Lack of vascular endothelial growth factor receptor-2/Flk1 signaling does not affect substantia nigra development.

    abstract::Oxygen tension is critical for proliferation of human and murine midbrain-derived neural precursor cells (mNPCs). Lack of hypoxia-inducible factor-1α (HIF1α) impairs midbrain dopaminergic neurogenesis which could be rescued by vascular endothelial growth factor (VEGF) via VEGFR-2 signaling. Here, we conditionally inac...

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    pub_type: 杂志文章


    authors: Hermann A,Loewenbrück KF,Boldt Ä,Fiedler C,Maisel M,Kalucka J,Schwarz J,Breier G,Storch A

    更新日期:2013-10-11 00:00:00

  • Lithium decreases turnover of arachidonate in several brain phospholipids.

    abstract::In vivo rates of incorporation and turnover of palmitate and arachidonate in brain phospholipids were measured in awake rats treated chronically with lithium, following intravenous infusion of radiolabeled palmitate and arachidonate, respectively. Chronic lithium, at a brain level considered to be therapeutic in human...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Chang MC,Grange E,Rabin O,Bell JM,Allen DD,Rapoport SI

    更新日期:1996-12-20 00:00:00

  • Neonatal handling (resilience) attenuates water-avoidance stress induced enhancement of chronic mechanical hyperalgesia in the rat.

    abstract::Chronic stress is well known to exacerbate pain. We tested the hypothesis that neonatal handling, which induces resilience to the negative impact of stress by increasing the quality and quantity of maternal care, attenuates the mechanical hyperalgesia produced by water-avoidance stress in the adult rat. Neonatal male ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Alvarez P,Levine JD,Green PG

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  • Protein tyrosine kinase inhibitors synergize with nerve growth factor in embryonic chick sensory neuronal cell survival.

    abstract::The survival of developing sensory neurons is dependent upon target-derived growth factors, in their absence neurons undergo programmed cell death. The molecular mechanisms underpinning neuronal cell survival and death are poorly understood. Tyrosine kinases are important signalling proteins that have been implicated ...

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    pub_type: 杂志文章


    authors: Bartlett SE

    更新日期:1997-05-16 00:00:00

  • Harmonic partials facilitate pitch discrimination in humans: electrophysiological and behavioral evidence.

    abstract::The effect of the spectral tone structure on pre-attentive and attentive pitch discrimination was investigated. The mismatch negativity (MMN) component was recorded from reading subjects to pitch changes of identical magnitude in pure tones with only one sinusoidal frequency component and in spectrally rich tones with...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Tervaniemi M,Ilvonen T,Sinkkonen J,Kujala A,Alho K,Huotilainen M,Näätänen R

    更新日期:2000-01-21 00:00:00

  • Loss of alpha power is related to increased gamma synchronization-A marker of reduced inhibition in tinnitus?

    abstract::Tinnitus is the perception of sound in the absence of any external auditory stimulus. Based on previous research we have proposed a framework which postulates that the reduction of ongoing inhibitory alpha activity in tinnitus subjects favors a synchronization of neurons in the gamma frequency range while in a resting...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Lorenz I,Müller N,Schlee W,Hartmann T,Weisz N

    更新日期:2009-04-10 00:00:00

  • Segregated distribution of nitric oxide synthase-positive cells in the periglomerular region of typical and atypical olfactory glomeruli.

    abstract::Using double-staining techniques, the distribution of NADPH-diaphorase (ND)- and nitric oxide synthase (NOS)-positive cells was compared in the periglomerular region of typical and atypical rat olfactory glomeruli. Dorsomedial and ventrolateral atypical glomeruli contained similar number of ND/NOS-positive periglomeru...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Crespo C,Porteros A,Arévalo R,Briñón JG,Aijón J,Alonso JR

    更新日期:1996-03-01 00:00:00

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    abstract:BACKGROUND:Diverse physiological and pathological functions of 27-hydroxycholesterol (27-OHC) were proved. However, cytotoxicity and potential influence of 27-OHC on cholesterol metabolism in neurons are unclear. DESIGN AND METHODS:In the vitro co-culture system, SH-SY5Y cells and C6 cells were applied to explore the ...

    journal_title:Neuroscience letters

    pub_type: 杂志文章


    authors: Wang H,Yuan L,Ma W,Han J,Lu Y,Feng L,Xiao R

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