Umbilical cord as an analytical matrix - A technical note.


:The umbilical cord (UC) connects the fetal blood supply to the placenta, so is exposed to all systemic endo- and xenobiotics. We have extensive experience using UC as an analytical matrix for detecting and/or quantitating drugs, chemicals and endogenous compounds. This technical note describes advantages (large amount available, ease of collection, small sample needed for use, rapid availability) and challenges (clinical relationships, processing difficulties, matrix effects on analytes and detection technologies) of UC as an analytical matrix in ELISA and LC/MS platforms, and provides guidance for successfully working with this tissue.






Price HR,Chehroudi C,Knight SJ,Smith AD,Lai D,Kim H,Wright TE,Coughtrie MW,Collier AC




Has Abstract


2020-01-15 00:00:00














  • The levels of hypoxia-regulated microRNAs in plasma of pregnant women with fetal growth restriction.

    abstract::MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression at the post-transcriptional level. While mostly intracellular, a portion of cellular miRNAs is released to the circulation and their level in the plasma is altered in certain pathological conditions such as cancer, and also during pregnancy. We...


    pub_type: 杂志文章


    authors: Mouillet JF,Chu T,Hubel CA,Nelson DM,Parks WT,Sadovsky Y

    更新日期:2010-09-01 00:00:00

  • Placental Site Trophoblastic Tumor with lung metastases as cause of death in a young patient: a case report.

    abstract::Placental Site Trophoblastic Tumor (PSTT) is a rare form of Gestational Trophoblastic Disease (GTD). Approximately 200 cases have been reported in the literature. This disease has unpredictable biological behavior, whereas approximately 10-15% have clinically malignant evolution. We present the case of a 21-year old w...


    pub_type: 杂志文章


    authors: Colecchi C,Partemi S,Minelli N,Cascini F,Rossi R,Fulcheri E,Pascali VL,Oliva A

    更新日期:2011-12-01 00:00:00

  • Maternal and fetal cellular relationships in the human placental basal plate.

    abstract::Maternal and fetal cellular relationships in the normal human term placental basal plate were investigated by single and double immunohistochemical labelling techniques. Extravillous fetal trophoblast in the basal plate was uniformly reactive with markers of low-molecular-weight cytokeratins. The predominant maternal ...


    pub_type: 杂志文章


    authors: Bulmer JN,Smith J,Morrison L,Wells M

    更新日期:1988-05-01 00:00:00

  • Sequential expression of VEGF and its receptors in human placental villi during very early pregnancy: differences between placental vasculogenesis and angiogenesis.

    abstract::Vascularization within the human placenta is the result of the de novo formation of vessels derived from pluripotent precursor cells in the mesenchymal core of the villi. Vascularization of placental villi starts at around day 21 post conception (p.c.) with a four somite embryo. At this stage progenitors of haemangiog...


    pub_type: 杂志文章


    authors: Demir R,Kayisli UA,Seval Y,Celik-Ozenci C,Korgun ET,Demir-Weusten AY,Huppertz B

    更新日期:2004-07-01 00:00:00

  • Reference weights for placentas delivered before the 28th week of gestation.

    abstract:CONTEXT:Very few studies have measured the weight of large numbers of placentas delivered before the 28th post-menstrual week. METHODS:We measured the weight of 930 singleton placentas delivered before the 28th post-menstrual week, and examined the distributions of weights in selected groups (week of gestation, reason...


    pub_type: 杂志文章


    authors: Hecht JL,Kliman HJ,Allred EN,Pflueger SM,Chang CH,Doss BJ,Roberts D,Livasy CA,Bhan I,Zambrano E,Ross DW,Senagore P,Husain AN,Leviton A

    更新日期:2007-10-01 00:00:00

  • Cyclic AMP as a second messenger for prorenin release from human decidual cells.

    abstract::The possible roles of cyclic AMP and protein kinase C in the release of renin from human decidual cells were investigated by examining renin release from monolayers of decidual cells exposed for 72 h to agents that increase intracellular cAMP or activate protein kinase C. Dibutyryl cAMP (10-1000 microM caused a dose-d...


    pub_type: 杂志文章


    authors: Poisner AM,Thrailkill K,Poisner R,Handwerger S

    更新日期:1991-05-01 00:00:00

  • Phenotype of the endothelium in the human term placenta.

    abstract::The placental endothelium contributes to regulating transplacental exchange and maintaining the immunological maternofetal barrier. We characterized the endothelial phenotype in human normal term placentae with a panel of antibodies to endothelial antigens using a standardized immunofluorescence method. Placental endo...


    pub_type: 杂志文章


    authors: Dye JF,Jablenska R,Donnelly JL,Lawrence L,Leach L,Clark P,Firth JA

    更新日期:2001-01-01 00:00:00

  • Altered uterine perfusion is involved in fetal outcome of diabetic rats.

    abstract::Maternal diabetes affects the development of the offspring by altering the uterine environment. We aimed to investigate the extent to which the blood flow (measured as Tissue Perfusion Units; TPU) to implantation sites and the expression of developmentally important genes in the offspring are affected by maternal diab...


    pub_type: 杂志文章


    authors: Zabihi S,Wentzel P,Eriksson UJ

    更新日期:2008-05-01 00:00:00

  • Identification and estimation of cell types in mixed primary cell cultures of early and term human placenta.

    abstract::Placental villi of early and term human placentae were dissociated with trypsin and mixed cell cultures were established. The different cell types were identified and estimated over a 14-day culture period using antibodies to keratin and vimentin filaments and their capacity to phagocytose yeast. The three main cell t...


    pub_type: 杂志文章


    authors: Contractor SF,Routledge A,Sooranna SR

    更新日期:1984-01-01 00:00:00

  • The effect of labour and placental separation on the shedding of syncytiotrophoblast microparticles, cell-free DNA and mRNA in normal pregnancy and pre-eclampsia.

    abstract::The clinical features of the maternal syndrome of pre-eclampsia can be explained by generalised maternal endothelial cell dysfunction, which is a part of a more global maternal systemic inflammatory response. There is growing evidence that these effects are associated with the shedding of cellular debris, including sy...


    pub_type: 杂志文章


    authors: Reddy A,Zhong XY,Rusterholz C,Hahn S,Holzgreve W,Redman CW,Sargent IL

    更新日期:2008-11-01 00:00:00

  • Human amnion mesenchymal cells are pro-inflammatory when activated by the Toll-like receptor 2/6 ligand, macrophage-activating lipoprotein-2.

    abstract:INTRODUCTION:Infection accounts for over 40% of preterm premature rupture of the fetal membranes (PPROM), a major cause of preterm birth. Toll-like receptors (TLR) play key roles in pathogen surveillance but their expression and function in amnion mesenchymal cells (AMC) is unclear. The aims of this study were to deter...


    pub_type: 杂志文章


    authors: Sato BL,Collier ES,Vermudez SA,Junker AD,Kendal-Wright CE

    更新日期:2016-08-01 00:00:00

  • Exploring sexual dimorphism in placental circulation at 22-24 weeks of gestation: A cross-sectional observational study.

    abstract:INTRODUCTION:Placental blood flow is closely associated with fetal growth and wellbeing. Recent studies suggest that there are differences in blood flow between male and female fetuses. We hypothesized that sexual dimorphism exists in fetal and placental blood flow at 22-24 weeks of gestation. METHODS:This was a prosp...


    pub_type: 杂志文章


    authors: Widnes C,Flo K,Acharya G

    更新日期:2017-01-01 00:00:00

  • Characterization of receptors for murine pregnancy specific glycoproteins 17 and 23.

    abstract::In primates and rodents, trophoblast cells synthesize and secrete into the maternal circulation a family of proteins known as pregnancy specific glycoproteins (PSG). The current study was undertaken to characterize the receptor for two members of the murine PSG family, PSG17 and PSG23. Binding of recombinant PSG17 and...


    pub_type: 杂志文章


    authors: Sulkowski GN,Warren J,Ha CT,Dveksler GS

    更新日期:2011-08-01 00:00:00

  • Plasma protein synthesis and secretion in the visceral yolk sac of the fetal rat: gene expression, protein synthesis and secretion.

    abstract::This report compares the relative levels of messenger RNA species coding for plasma proteins in rat visceral yolk sac and fetal liver from 12.5 days to 21.5 days gestation. Transthyretin, retinol-binding protein, transferrin and alpha 1-fetoprotein mRNAs were detected in both tissues, although relative levels were muc...


    pub_type: 杂志文章


    authors: Thomas T,Southwell BR,Schreiber G,Jaworowski A

    更新日期:1990-09-01 00:00:00

  • Early placentation in the dusky leaf monkey (Presbytis obscura).

    abstract::Implantation in the dusky leaf monkey, Presbytis obscura, is superficial; the placenta is of the double discoid villous haemochorial type. Immediately prior to implantation the thick endometrium contains highly active uterine glands. There is an early uterine epithelial proliferation at the implantation site but no st...


    pub_type: 杂志文章


    authors: Burton GJ

    更新日期:1980-07-01 00:00:00

  • Review: Modelling placental amino acid transfer--from transporters to placental function.

    abstract::Amino acid transfer to the fetus is dependent on several different factors. While these factors can be understood in isolation, it is still not possible to predict the function of the system as a whole. In order to do this an integrated approach is required which incorporates the interactions between the different det...


    pub_type: 杂志文章,评审


    authors: Lewis RM,Brooks S,Crocker IP,Glazier J,Hanson MA,Johnstone ED,Panitchob N,Please CP,Sibley CP,Widdows KL,Sengers BG

    更新日期:2013-03-01 00:00:00

  • Role of low placental share in twin-twin transfusion syndrome complicated by intrauterine growth restriction.

    abstract:OBJECTIVES:Prior studies have demonstrated that donor twin survival following treatment of twin-twin transfusion syndrome (TTTS) was highly associated with donor intrauterine growth restriction (IUGR). Here, we hypothesized that donor IUGR may be attributed in part to low placental share. STUDY DESIGN:The study popula...


    pub_type: 杂志文章


    authors: Grubbs BH,Benirschke K,Korst LM,Llanes A,Yedigarova L,Chmait RH

    更新日期:2011-08-01 00:00:00

  • A new method for measurement of placental elasticity: acoustic radiation force impulse imaging.

    abstract:INTRODUCTION:The velocities of the lateral shear waves (Vs; m s⁻¹) generated by an acoustic radiation force impulse (ARFI) correlate with Young's modulus. Therefore, ARFI can be used as a new method to evaluate tissue elasticity. The aim of this study was to investigate the safety of ARFI imaging and the differences in...


    pub_type: 杂志文章


    authors: Sugitani M,Fujita Y,Yumoto Y,Fukushima K,Takeuchi T,Shimokawa M,Kato K

    更新日期:2013-11-01 00:00:00

  • Upregulation of VEGF by small activating RNA and its implications in preeclampsia.

    abstract:INTRODUCTION:Preeclampsia is a severe pregnancy complication mostly due to inadequate vascular dilation and remodeling of spiral arteries. VEGF, the major factor for angiogenesis, is necessary for modulating angiogenic processes in the placenta. Hence reduction of VEGF in gestational hypertension may also lead to hypop...


    pub_type: 杂志文章


    authors: Guo X,Feng L,Jia J,Chen R,Yu J

    更新日期:2016-10-01 00:00:00

  • Matrix metalloproteinase 1 in pre-eclampsia and fetal growth restriction: reduced gene expression in decidual tissue and protein expression in extravillous trophoblasts.

    abstract::Superficial invasion of extravillous trophoblasts (EVTs) and impaired spiral artery remodelling are characterizing phenomena in pregnancies complicated by pre-eclampsia (PE) and fetal growth restriction (FGR). However, the underlying causes remain unclear. In this study, gene expression in decidua basalis tissue from ...


    pub_type: 杂志文章


    authors: Lian IA,Toft JH,Olsen GD,Langaas M,Bjørge L,Eide IP,Børdahl PE,Austgulen R

    更新日期:2010-07-01 00:00:00

  • Histological distribution pattern of hemosiderin deposition on the chorionic plate and fetal membrane of diffuse chorioamniotic hemosiderosis related to chronic abruption oligohydramnios sequence.

    abstract:INTRODUCTION:Chronic abruption oligohydramnios sequence (CAOS) is histologically characterized by diffuse chorioamniotic hemosiderosis (DCH). However, the criteria for the histological evaluation of the extent of CAOS-related hemosiderin deposition (HD) of the membranes and the difference in HD between the chorionic pl...


    pub_type: 杂志文章


    authors: Ishida A,Minamiguchi S,Yamada Y,Nakagawa R,Chigusa Y,Kondoh E,Mandai M,Haga H

    更新日期:2021-01-20 00:00:00

  • Oxygen tension modulates AQP9 expression in human placenta.

    abstract:UNLABELLED:Placental hypoxia has been implicated in pregnancy pathologies such as preeclampsia. We have previously reported that AQP9 is highly expressed in syncytiotrophoblast from normal placentas and shows an overexpression in preeclamptic placentas, with a lack of functionality for water transport. Up to now, the r...


    pub_type: 杂志文章


    authors: Castro-Parodi M,Szpilbarg N,Dietrich V,Sordelli M,Reca A,Abán C,Maskin B,Farina MG,Damiano AE

    更新日期:2013-08-01 00:00:00

  • Effects of nifedipine and sildenafil on placental hemodynamics and gas exchange during fetal hypoxemia in a chronic sheep model.

    abstract:INTRODUCTION:We hypothesized that nifedipine and sildenafil would have no detrimental effects on placental hemodynamics and gas exchange under fetal hypoxemia. METHODS:In 33 chronically instrumented fetal sheep, placental volume blood flow (QPlac) and umbilical artery (UA) vascular impedance were measured by Doppler u...


    pub_type: 杂志文章


    authors: Alanne L,Bhide A,Hoffren J,Lantto J,Huhta H,Kokki M,Haapsamo M,Acharya G,Räsänen J

    更新日期:2020-01-15 00:00:00

  • Regulation of placental efficiency for nutrient transport by imprinted genes.

    abstract::Intrauterine growth and development can impact upon the long-term health of an individual. The fetus is dependent upon the placenta for its supply of nutrients and oxygen from the mother. In turn, the functional capacity of the placenta to supply that demand is under the control of the fetal and maternal genomes. Rece...


    pub_type: 杂志文章,评审


    authors: Angiolini E,Fowden A,Coan P,Sandovici I,Smith P,Dean W,Burton G,Tycko B,Reik W,Sibley C,Constância M

    更新日期:2006-04-01 00:00:00

  • The placenta in leprosy.

    abstract::Eighty-one placentae from women with leprosy and 17 placentae from healthy controls were subjected to a detailed macroscopic, light microscopic, ultrastructural, immunopathological, microbiological and biochemical study. The placental morphology and immunohistology were normal, and there was no morphological evidence ...


    pub_type: 杂志文章


    authors: Duncan ME,Fox H,Harkness RA,Rees RJ

    更新日期:1984-05-01 00:00:00

  • SNAT expression in rat placenta.

    abstract::Amino acid transport System A (SysA) activity is present within the rodent and human placentas. Inhibition of this transport system is associated with fetal growth retardation. Several cDNAs encoding SysA transport proteins have been discovered, and their presence documented within the human placenta. We have demonstr...


    pub_type: 杂志文章


    authors: Novak D,Lehman M,Bernstein H,Beveridge M,Cramer S

    更新日期:2006-04-01 00:00:00

  • Histological changes in the umbilical artery following severe chorioamnionitis and funisitis may be indicative of early atherosclerosis.

    abstract::We investigated whether histological evidence of early atherosclerosis was present in the umbilical artery of 21 pregnancies complicated by severe perinatal inflammation, and 21 controls matched for gestational age, sex and birth weight. Severe chorioamnionitis with funisitis was associated with increased numbers of C...


    pub_type: 杂志文章


    authors: Rafferty AR,D'Arcy C,Cann L,Pyman J,Rogers P,Davis PG,Nowell C,Burgner D

    更新日期:2017-02-01 00:00:00

  • Maternal high-fat diet sex-specifically alters placental morphology and transcriptome in rats: Assessment by next-generation sequencing.

    abstract:INTRODUCTION:Maternal nutrition is an extremely important health issue. We evaluated the impact of maternal high fat diet (HFD) on pregnancy outcomes, elucidated how the rat placenta and fetus respond to diet manipulation based on fetal sex, and identified candidate genes and pathways. METHODS:Rats were fed a normal o...


    pub_type: 杂志文章


    authors: Lin YJ,Huang LT,Tsai CC,Sheen JM,Tiao MM,Yu HR,Lin IC,Tain YL

    更新日期:2019-03-01 00:00:00

  • Effect of ethanol on human placental transport and metabolism of adenosine.

    abstract::It has been suggested that adenosine is involved in the acute effects of ethanol in a number of tissues. The present study was undertaken to evaluate the role of adenosine on the vascular responses of perfused isolated human placental cotyledons after the acute administration of ethanol. The possibility that ethanol m...


    pub_type: 杂志文章


    authors: Acevedo CG,Huambachano A,Perez E,Rojas S,Bravo I,Contreras E

    更新日期:1997-07-01 00:00:00

  • Animal models of fetal growth restriction: Considerations for translational medicine.

    abstract::Fetal growth restriction (FGR) is the failure of a fetus to reach its full genetic growth potential. It occurs in up to 8% of pregnancies, and after premature birth is the second leading cause of infant mortality and morbidity. There is no treatment currently available for FGR. Its primary cause, when not attributable...


    pub_type: 杂志文章


    authors: Swanson AM,David AL

    更新日期:2015-06-01 00:00:00