The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer.


:As key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired outcome at the point-of-care. To address the need, we have designed and fabricated an easy-to-use, accurate, and portable impedance aggregometer called "MICELI" (MICrofluidic, ELectrical, Impedance). It improves on current platelet aggregation technology by decreasing footprint, assay complexity, and time to obtain results. The current study aimed to optimize the MICELI protocol; validate sensitivity to aggregation agonists and key blood parameters, i.e., platelet count and hematocrit; and verify the MICELI operational performance as compared to commercial impedance aggregometry. We demonstrated that the MICELI aggregometer could detect platelet aggregation in 250 μL of whole blood or platelet-rich plasma, stimulated by ADP, TRAP-6, collagen, epinephrine, and calcium ionophore. Using hirudin as blood anticoagulant allowed higher aggregation values. Aggregation values obtained by the MICELI strongly correlated with platelet count and were not affected by hematocrit. The operational performance comparison of the MICELI and the Multiplate® Analyzer demonstrated strong correlation and similar interdonor distribution of aggregation values obtained between these devices. With the proven reliability of the data obtained by the MICELI aggregometer, it can be further translated into a point-of-care diagnostic device aimed at monitoring platelet function in order to guide pharmacological hemostasis management and platelet transfusions.


Int J Mol Sci


Roka-Moiia Y,Bozzi S,Ferrari C,Mantica G,Dimasi A,Rasponi M,Santoleri A,Scavone M,Consolo F,Cattaneo M,Slepian MJ,Redaelli A




Has Abstract


2020-02-11 00:00:00










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    pub_type: 杂志文章,评审


    authors: Sato T,Kawasaki T,Mine S,Matsumura H

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    authors: Kiseleva O,Ponomarenko E,Poverennaya E

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    更新日期:2014-08-05 00:00:00

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    authors: Cammarata FP,Forte GI,Broggi G,Bravatà V,Minafra L,Pisciotta P,Calvaruso M,Tringali R,Tomasello B,Torrisi F,Petringa G,Cirrone GAP,Cuttone G,Acquaviva R,Caltabiano R,Russo G

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  • A Novel Selective 11β-HSD1 Inhibitor, (E)-4-(2-(6-(2,6-Dichloro-4-(Trifluoromethyl)Phenyl)-4-Methyl-1,1-Dioxido-1,2,6-Thiadiazinan-2-yl)Acetamido)Adamantan-1-Carboxamide (KR-67607), Prevents BAC-Induced Dry Eye Syndrome.

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    pub_type: 临床试验,杂志文章


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


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    journal_title:International journal of molecular sciences

    pub_type: 社论


    authors: Stone MJ

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chinigarzadeh A,Kasim NF,Muniandy S,Kassim NM,Salleh N

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gryz E,Perlińska-Lenart U,Gawarecka K,Jozwiak A,Piłsyk S,Lipko A,Jemiola-Rzeminska M,Bernat P,Muszewska A,Steczkiewicz K,Ginalski K,Długoński J,Strzalka K,Swiezewska E,Kruszewska JS

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    pub_type: 杂志文章


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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Sánchez A,Castro C,Flores DL,Gutiérrez E,Baldi P

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


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    更新日期:2020-12-29 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Souto SB,Campos JR,Fangueiro JF,Silva AM,Cicero N,Lucarini M,Durazzo A,Santini A,Souto EB

    更新日期:2020-01-18 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dony N,Crowet JM,Joris B,Brasseur R,Lins L

    更新日期:2013-05-30 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Frisch A,Kälin S,Monk R,Radke J,Heppner FL,Kälin RE

    更新日期:2020-06-11 00:00:00

  • Needle in a Haystack: The Naïve Repertoire as a Source of T Cell Receptors for Adoptive Therapy with Engineered T Cells.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: D'Ippolito E,Wagner KI,Busch DH

    更新日期:2020-11-06 00:00:00

  • Insect Transcription Factors: A Landscape of Their Structures and Biological Functions in Drosophila and beyond.

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Guo Z,Qin J,Zhou X,Zhang Y

    更新日期:2018-11-21 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Williams CH,Hong CC

    更新日期:2011-01-01 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Wang R,Sun H,Wang G,Ren H

    更新日期:2020-09-29 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ding Q,Zhang F,Feng Y,Wang H

    更新日期:2020-12-07 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kotzer-Nevo H,de Lima Alves F,Rappsilber J,Sperling J,Sperling R

    更新日期:2014-06-30 00:00:00