Abstract:
:Notch signaling plays a critical role in the development and function of macrophages. The aim of the present study was to investigate the relationship between Notch signaling pathway and macrophage apoptosis after LPS stimulation. In RAW 264.7 cells, the mRNA expression of Jagged1, Hes1, Hes 5 and GM-CSF, and protein expression of NICD1 and GM-CSF were increased after LPS stimulation. Inhibition of Notch signaling by γ-secretase inhibitor DAPT and the suppression of Notch1 expression using siRNA both significantly prevented LPS induced activation of JNK and NF-kB, and simultaneously the expression of GM-CSF was also down regulated significantly. JNK inhibitor SP600125 was used to block the phosphorylation of JNK signaling, Western blot results showed that the activation of NF-kB was blocked and expression of GM-CSF was down-regulated. Finally, flow cytometry analyses showed that the Notch signaling was involved in the regulation of macrophage apoptosis after LPS stimulation. Our study showed that the Notch signaling pathway was activated and involved in the regulation of macrophage apoptosis after LPS stimulation through JNK/ NF-kB signaling regulated GM-CSF expression.
journal_name
J Biol Regul Homeost Agentsjournal_title
Journal of biological regulators and homeostatic agentsauthors
Chen GQ,Wang QM,Yu M,Cheng YD,Zhang ZC,Wang WS,Qiu Y,Sun LH,Peng K,Yang Hdoi
10.23812/19-283-Asubject
Has Abstractpub_date
2020-02-12 00:00:00issue
1eissn
0393-974Xissn
1724-6083pii
04journal_volume
34pub_type
杂志文章abstract::Various hematopoietic malignant disorders have been shown to bear chromosome abnormalities of the 11q23 band, which can be rearranged with many different chromosomal regions in a wide variety of different leukemia subtypes. Several laboratories have identified a trithorax-related gene that is involved in most of the 1...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
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abstract::IL-32, a newly-discovered proinflammatory cytokine that activates the p38MAPK and NF-kappaB pathways, is an important player in innate and adaptive immune response. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the productio...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论,评审
doi:
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-03-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
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更新日期:2013-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2019-03-01 00:00:00
abstract:: ...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2019-01-01 00:00:00
abstract::Given the high sensitivity of the male reproductive system to oxidative stress and to temperature changes, the amount of germ cell apoptosis and the activation of the poly(ADP-ribosyl)ation system (a very sensitive index of genotoxic stress) were evaluated in the testicular tissue of adult rats which underwent a 10-wk...
journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
doi:
更新日期:2015-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2016-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2016-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
更新日期:2020-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2018-05-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 临床试验
doi:
更新日期:2020-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,随机对照试验
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更新日期:2014-07-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2010-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 社论
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更新日期:2016-10-01 00:00:00
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 杂志文章,评审
doi:
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journal_title:Journal of biological regulators and homeostatic agents
pub_type: 信件
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