Lysosome biology in autophagy.


:Autophagy is a major intracellular degradation system that derives its degradative abilities from the lysosome. The most well-studied form of autophagy is macroautophagy, which delivers cytoplasmic material to lysosomes via the double-membraned autophagosome. Other forms of autophagy, namely chaperone-mediated autophagy and microautophagy, occur directly on the lysosome. Besides providing the means for degradation, lysosomes are also involved in autophagy regulation and can become substrates of autophagy when damaged. During autophagy, they exhibit notable changes, including increased acidification, enhanced enzymatic activity, and perinuclear localization. Despite their importance to autophagy, details on autophagy-specific regulation of lysosomes remain relatively scarce. This review aims to provide a summary of current understanding on the behaviour of lysosomes during autophagy and outline unexplored areas of autophagy-specific lysosome research.


Cell Discov


Cell discovery


Yim WW,Mizushima N




Has Abstract


2020-02-11 00:00:00










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    abstract::The use of antibodies to target immune checkpoints, particularly PD-1/PD-L1, has made a profound impact in the field of cancer immunotherapy. Here, we identified KN035, an anti-PD-L1 nanobody that can strongly induce T-cell responses and inhibit tumor growth. The crystal structures of KN035 complexed with PD-L1 and fr...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Zhang F,Wei H,Wang X,Bai Y,Wang P,Wu J,Jiang X,Wang Y,Cai H,Xu T,Zhou A

    更新日期:2017-03-07 00:00:00

  • Structural insight into the arginine-binding specificity of CASTOR1 in amino acid-dependent mTORC1 signaling.

    abstract::The mechanistic Target Of Rapamycin Complex 1 (mTORC1) is central to the cellular response to changes in nutrient signals such as amino acids. CASTOR1 is shown to be an arginine sensor, which plays an important role in the activation of the mTORC1 pathway. In the deficiency of arginine, CASTOR1 interacts with GATOR2, ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Xia J,Wang R,Zhang T,Ding J

    更新日期:2016-09-13 00:00:00

  • Structural insight into the Ragulator complex which anchors mTORC1 to the lysosomal membrane.

    abstract::The mechanistic target of rapamycin (mTOR) signal-transduction pathway plays a key role in regulating many aspects of metabolic processes. The central player of the mTOR signaling pathway, mTOR complex 1 (mTORC1), is recruited by the pentameric Ragulator complex and the heterodimeric Rag GTPase complex to the lysosoma...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Mu Z,Wang L,Deng W,Wang J,Wu G

    更新日期:2017-12-26 00:00:00

  • Methylation of transcription factor YY2 regulates its transcriptional activity and cell proliferation.

    abstract::Yin Yang 1 (YY1) is a multifunctional DNA-binding transcription factor shown to be critical in a variety of biological processes, and its activity and function have been shown to be regulated by multitude of mechanisms, which include but are not limited to post-translational modifications (PTMs), its associated protei...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Wu XN,Shi TT,He YH,Wang FF,Sang R,Ding JC,Zhang WJ,Shu XY,Shen HF,Yi J,Gao X,Liu W

    更新日期:2017-10-03 00:00:00

  • Network-based drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2.

    abstract::Human coronaviruses (HCoVs), including severe acute respiratory syndrome coronavirus (SARS-CoV) and 2019 novel coronavirus (2019-nCoV, also known as SARS-CoV-2), lead global epidemics with high morbidity and mortality. However, there are currently no effective drugs targeting 2019-nCoV/SARS-CoV-2. Drug repurposing, re...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Zhou Y,Hou Y,Shen J,Huang Y,Martin W,Cheng F

    更新日期:2020-03-16 00:00:00

  • Long-term consumption of caffeine-free high sucrose cola beverages aggravates the pathogenesis of EAE in mice.

    abstract::Epidemiological data provide strong evidence of dramatically increasing incidences of many autoimmune diseases in the past few decades, mainly in western and westernized countries. Recent studies clearly revealed that 'Western diet' increases the risk of autoimmune diseases at least partially via disrupting intestinal...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Cao G,Wang Q,Huang W,Tong J,Ye D,He Y,Liu Z,Tang X,Cheng H,Wen Q,Li D,Chau HT,Wen Y,Zhong H,Meng Z,Liu H,Wu Z,Zhao L,Flavell RA,Zhou H,Xu A,Yang H,Yin Z

    更新日期:2017-06-20 00:00:00

  • Erratum: Author Correction: Immune cell profiling of COVID-19 patients in the recovery stage by single-cell sequencing.

    abstract::[This corrects the article DOI: 10.1038/s41421-020-0168-9.]. ...

    journal_title:Cell discovery

    pub_type: 杂志文章,已发布勘误


    authors: Wen W,Su W,Tang H,Le W,Zhang X,Zheng Y,Liu X,Xie L,Li J,Ye J,Dong L,Cui X,Miao Y,Wang D,Dong J,Xiao C,Chen W,Wang H

    更新日期:2020-06-20 00:00:00

  • Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss.

    abstract::Maintaining homeostasis of the decidual immune microenvironment at the maternal-fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leuko...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Guo C,Cai P,Jin L,Sha Q,Yu Q,Zhang W,Jiang C,Liu Q,Zong D,Li K,Fang J,Lu F,Wang Y,Li D,Lin J,Li L,Zeng Z,Tong X,Wei H,Qu K

    更新日期:2021-01-04 00:00:00

  • Targeting the γ-/β-secretase interaction reduces β-amyloid generation and ameliorates Alzheimer's disease-related pathogenesis.

    abstract::Despite decades of intense global effort, no disease-modifying drugs for Alzheimer's disease have emerged. Molecules targeting catalytic activities of γ-secretase or β-site APP-cleaving enzyme 1 (BACE1) have been beset by undesired side effects. We hypothesized that blocking the interaction between BACE1 and γ-secreta...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Cui J,Wang X,Li X,Wang X,Zhang C,Li W,Zhang Y,Gu H,Xie X,Nan F,Zhao J,Pei G

    更新日期:2015-08-18 00:00:00

  • DNA methylation reprogramming of functional elements during mammalian embryonic development.

    abstract::DNA methylation plays important roles during development. However, the DNA methylation reprogramming of functional elements has not been fully investigated during mammalian embryonic development. Herein, using our modified MethylC-Seq library generation method and published post-bisulphite adapter-tagging (PBAT) metho...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Li C,Fan Y,Li G,Xu X,Duan J,Li R,Kang X,Ma X,Chen X,Ke Y,Yan J,Lian Y,Liu P,Zhao Y,Zhao H,Chen Y,Yu Y,Liu J

    更新日期:2018-08-07 00:00:00

  • PBR1 selectively controls biogenesis of photosynthetic complexes by modulating translation of the large chloroplast gene Ycf1 in Arabidopsis.

    abstract::The biogenesis of photosystem I (PSI), cytochrome b 6 f (Cytb 6 f) and NADH dehydrogenase (NDH) complexes relies on the spatially and temporally coordinated expression and translation of both nuclear and chloroplast genes. Here we report the identification of photosystem biogenesis regulator 1 (PBR1), a nuclear-encode...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Yang XF,Wang YT,Chen ST,Li JK,Shen HT,Guo FQ

    更新日期:2016-05-10 00:00:00

  • Heparan sulfate assists SARS-CoV-2 in cell entry and can be targeted by approved drugs in vitro.

    abstract::The cell entry of SARS-CoV-2 has emerged as an attractive drug repurposing target for COVID-19. Here we combine genetics and chemical perturbation to demonstrate that ACE2-mediated entry of SARS-Cov and CoV-2 requires the cell surface heparan sulfate (HS) as an assisting cofactor: ablation of genes involved in HS bios...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Zhang Q,Chen CZ,Swaroop M,Xu M,Wang L,Lee J,Wang AQ,Pradhan M,Hagen N,Chen L,Shen M,Luo Z,Xu X,Xu Y,Huang W,Zheng W,Ye Y

    更新日期:2020-11-04 00:00:00

  • TRPA1 channel mediates organophosphate-induced delayed neuropathy.

    abstract::The organophosphate-induced delayed neuropathy (OPIDN), often leads to paresthesias, ataxia and paralysis, occurs in the late-stage of acute poisoning or after repeated exposures to organophosphate (OP) insecticides or nerve agents, and may contribute to the Gulf War Syndrome. The acute phase of OP poisoning is often ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Ding Q,Fang S,Chen X,Wang Y,Li J,Tian F,Xu X,Attali B,Xie X,Gao Z

    更新日期:2017-08-01 00:00:00

  • Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to γ-secretase cleavage and the Aβ42/Aβ40 ratio.

    abstract::Mutations in the amyloid precursor protein (APP) gene and the aberrant cleavage of APP by γ-secretase are associated with Alzheimer's disease (AD). Here we have developed a simple and sensitive cell-based assay to detect APP cleavage by γ-secretase. Unexpectedly, most familial AD (FAD)-linked APP mutations make APP pa...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Xu TH,Yan Y,Kang Y,Jiang Y,Melcher K,Xu HE

    更新日期:2016-08-23 00:00:00

  • Identification of novel genetic variants predisposing to familial oral squamous cell carcinomas.

    abstract::Oral squamous cell carcinoma (OSCC) is a common subtype of head and neck squamous cell carcinoma (HNSCC), but the pathogenesis underlying familial OSCCs is unknown. Here, we analyzed whole-genome sequences of a family with autosomal dominant expression of oral tongue cancer and identified proto-oncogenes VAV2 and IQGA...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Huang Y,Zhao J,Mao G,Lee GS,Zhang J,Bi L,Gu L,Chang Z,Valentino J,Li GM

    更新日期:2019-11-26 00:00:00

  • A role for ErbB signaling in the induction of reactive astrogliosis.

    abstract::Reactive astrogliosis is a hallmark of many neurological disorders, yet its functions and molecular mechanisms remain elusive. Particularly, the upstream signaling that regulates pathological responses of astrocytes is largely undetermined. We used a mouse traumatic brain injury model to induce astrogliosis and reveal...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Chen J,He W,Hu X,Shen Y,Cao J,Wei Z,Luan Y,He L,Jiang F,Tao Y

    更新日期:2017-12-05 00:00:00

  • A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer.

    abstract::Aberrant activation of Wnt/β-catenin signaling has been associated with the onset and progression of many types of tumors and thus β-catenin represents one attractive intracellular target for cancer therapy. Based on the Axin-derived peptide that binds to β-catenin, two stapled peptides SAHPA1 and xStAx were reported ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Liao H,Li X,Zhao L,Wang Y,Wang X,Wu Y,Zhou X,Fu W,Liu L,Hu HG,Chen YG

    更新日期:2020-06-09 00:00:00

  • ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells.

    abstract::Loss of organelle homeostasis is a hallmark of aging. However, it remains elusive how this occurs at gene expression level. Here, we report that human mesenchymal stem cell (hMSC) aging is associated with dysfunction of double-membrane organelles and downregulation of transcription factor ATF6. CRISPR/Cas9-mediated in...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Wang S,Hu B,Ding Z,Dang Y,Wu J,Li D,Liu X,Xiao B,Zhang W,Ren R,Lei J,Hu H,Chen C,Chan P,Li D,Qu J,Tang F,Liu GH

    更新日期:2018-01-05 00:00:00

  • Malat1 regulates myogenic differentiation and muscle regeneration through modulating MyoD transcriptional activity.

    abstract::Malat1 is one of the most abundant long non-coding RNAs in various cell types; its exact cellular function is still a matter of intense investigation. In this study we characterized the function of Malat1 in skeletal muscle cells and muscle regeneration. Utilizing both in vitro and in vivo assays, we demonstrate that ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Chen X,He L,Zhao Y,Li Y,Zhang S,Sun K,So K,Chen F,Zhou L,Lu L,Wang L,Zhu X,Bao X,Esteban MA,Nakagawa S,Prasanth KV,Wu Z,Sun H,Wang H

    更新日期:2017-03-14 00:00:00

  • Metformin-induced ablation of microRNA 21-5p releases Sestrin-1 and CAB39L antitumoral activities.

    abstract::Metformin is a commonly prescribed type II diabetes medication that exhibits promising anticancer effects. Recently, these effects were found to be associated, at least in part, with a modulation of microRNA expression. However, the mechanisms by which single modulated microRNAs mediate the anticancer effects of metfo...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Pulito C,Mori F,Sacconi A,Goeman F,Ferraiuolo M,Pasanisi P,Campagnoli C,Berrino F,Fanciulli M,Ford RJ,Levrero M,Pediconi N,Ciuffreda L,Milella M,Steinberg GR,Cioce M,Muti P,Strano S,Blandino G

    更新日期:2017-07-04 00:00:00

  • Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry.

    abstract::The re-emergence of Zika virus (ZIKV) and Ebola virus (EBOV) poses serious and continued threats to the global public health. Effective therapeutics for these maladies is an unmet need. Here, we show that emetine, an anti-protozoal agent, potently inhibits ZIKV and EBOV infection with a low nanomolar half maximal inhi...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Yang S,Xu M,Lee EM,Gorshkov K,Shiryaev SA,He S,Sun W,Cheng YS,Hu X,Tharappel AM,Lu B,Pinto A,Farhy C,Huang CT,Zhang Z,Zhu W,Wu Y,Zhou Y,Song G,Zhu H,Shamim K,Martínez-Romero C,García-Sastre A,Preston RA,Ja

    更新日期:2018-06-05 00:00:00

  • Mixed-lineage leukemia protein 2 suppresses ciliary assembly by the modulation of actin dynamics and vesicle transport.

    abstract::Primary cilia are critically involved in the coordination of diverse signaling pathways and ciliary defects are associated with a variety of human diseases. The past decades have witnessed great progress in the core machinery orchestrating ciliary assembly. However, the upstream epigenetic cues that direct ciliogenesi...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Yang Y,Hao H,Wu X,Guo S,Liu Y,Ran J,Li T,Li D,Liu M,Zhou J

    更新日期:2019-06-25 00:00:00

  • STK11 is required for the normal program of ciliated cell differentiation in airways.

    abstract::The functional properties of mucosal surfaces are dependent on establishing the correct proportions of specialized epithelial cell types. Multiciliated cells (also known as ciliated cells) are evolutionarily conserved and functionally indispensable epithelial cells, as suggested by the link between ciliated cell dysfu...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Chu Q,Yao C,Qi X,Stripp BR,Tang N

    更新日期:2019-07-16 00:00:00

  • Intronic cleavage and polyadenylation regulates gene expression during DNA damage response through U1 snRNA.

    abstract::The DNA damage response involves coordinated control of gene expression and DNA repair. Using deep sequencing, we found widespread changes of alternative cleavage and polyadenylation site usage on ultraviolet-treatment in mammalian cells. Alternative cleavage and polyadenylation regulation in the 3' untranslated regio...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Devany E,Park JY,Murphy MR,Zakusilo G,Baquero J,Zhang X,Hoque M,Tian B,Kleiman FE

    更新日期:2016-06-14 00:00:00

  • A novel miR-200b-3p/p38IP pair regulates monocyte/macrophage differentiation.

    abstract::Monocyte/macrophage differentiation represents a major branch of hematopoiesis and is a central event in the immune response, but the molecular mechanisms underlying are not fully delineated. Here we show that p38 mitogen-activated protein kinase (MAPK) interacting protein (p38IP) is downregulated during monocyte/macr...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Yu X,Wang QL,Li YF,Wang XD,Xu A,Li Y

    更新日期:2016-01-26 00:00:00

  • Increased dysbindin-1B isoform expression in schizophrenia and its propensity in aggresome formation.

    abstract::Genetic variations in the human dysbindin-1 gene (DTNBP1) have been associated with schizophrenia. As a result of alternative splicing, the human DTNBP1 gene generates at least three distinct protein isoforms, dysbindin-1A, -1B and -1C. Significant effort has focused on dysbindin-1A, an important player in multiple st...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Xu Y,Sun Y,Ye H,Zhu L,Liu J,Wu X,Wang L,He T,Shen Y,Wu JY,Xu Q

    更新日期:2015-11-10 00:00:00

  • Two approaches reveal a new paradigm of 'switchable or genetics-influenced allele-specific DNA methylation' with potential in human disease.

    abstract::Imprinted genes are vulnerable to environmental influences during early embryonic development, thereby contributing to the onset of disease in adulthood. Monoallelic methylation at several germline imprints has been reported as DNMT1-dependent. However, which of these two epigenetic attributes, DNMT1-dependence or all...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Martos SN,Li T,Ramos RB,Lou D,Dai H,Xu JC,Gao G,Gao Y,Wang Q,An C,Zhang X,Jia Y,Dawson VL,Dawson TM,Ji H,Wang Z

    更新日期:2017-11-14 00:00:00

  • A growth factor-free culture system underscores the coordination between Wnt and BMP signaling in Lgr5+ intestinal stem cell maintenance.

    abstract::Lgr5+ intestinal stem cells (ISCs) drive the fast renewal of intestinal epithelium. Several signaling pathways have been shown to regulate ISC fates. However, it is unclear what are the essential signals to sustain the ISC self-renewal. Here we show that coordination between Wnt and BMP signaling activity is necessary...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Li Y,Liu Y,Liu B,Wang J,Wei S,Qi Z,Wang S,Fu W,Chen YG

    更新日期:2018-09-04 00:00:00

  • mTORC1 regulates mannose-6-phosphate receptor transport and T-cell vulnerability to regulatory T cells by controlling kinesin KIF13A.

    abstract::Mannose-6-phosphate receptor (M6PR) that facilitates cellular uptake of M6P-bearing proteins, including serine-protease granzyme-B (Gzm-B) has an important role in T-cell activation, migration and contraction. However, molecular mechanisms controlling M6PR expression in T cells remain poorly understood. Here, we show ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Ahmed KA,Xiang J

    更新日期:2017-04-25 00:00:00

  • Bloom syndrome complex promotes FANCM recruitment to stalled replication forks and facilitates both repair and traverse of DNA interstrand crosslinks.

    abstract::The recruitment of FANCM, a conserved DNA translocase and key component of several DNA repair protein complexes, to replication forks stalled by DNA interstrand crosslinks (ICLs) is a step upstream of the Fanconi anemia (FA) repair and replication traverse pathways of ICLs. However, detection of the FANCM recruitment ...

    journal_title:Cell discovery

    pub_type: 杂志文章


    authors: Ling C,Huang J,Yan Z,Li Y,Ohzeki M,Ishiai M,Xu D,Takata M,Seidman M,Wang W

    更新日期:2016-12-20 00:00:00