Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation.

Abstract:

:Mammalian cells present a fingerprint of their proteome to the adaptive immune system through the display of endogenous peptides on MHC-I complexes. MHC-I-bound peptides originate from protein degradation by the proteasome, suggesting that stably folded, long-lived proteins could evade monitoring. Here, we investigate the role in antigen presentation of the ribosome-associated quality control (RQC) pathway for the degradation of nascent polypeptides that are encoded by defective messenger RNAs and undergo stalling at the ribosome during translation. We find that degradation of model proteins by RQC results in efficient MHC-I presentation, independent of their intrinsic folding properties. Quantitative profiling of MHC-I peptides in wild-type and RQC-deficient cells by mass spectrometry showed that RQC substantially contributes to the composition of the immunopeptidome. Our results also identify endogenous substrates of the RQC pathway in human cells and provide insight into common principles causing ribosome stalling under physiological conditions.

authors

Trentini DB,Pecoraro M,Tiwary S,Cox J,Mann M,Hipp MS,Hartl FU

doi

10.1073/pnas.1914401117

subject

Has Abstract

pub_date

2020-02-25 00:00:00

pages

4099-4108

issue

8

eissn

0027-8424

issn

1091-6490

pii

1914401117

journal_volume

117

pub_type

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