Abstract:
INTRODUCTION:The identification of sentinel lymph nodes (SLNs) is important in deciding the resection range during surgery. 99mTc-labeled mannosylated human serum albumin ([99mTc]Tc-MSA) is a radiopharmaceutical developed for SLN detection by targeting macrophages. Evans blue (EB) is a blue dye binding strongly to albumin and has been used for SLN detection. [99mTc]Tc-MSA-EB conjugate was prepared as a multi-modal imaging agent and tested its performance by visual investigation, fluorescence imaging and SPECT/CT for SLN mapping in mice. METHODS:EB was mixed with various concentration of MSA to prepare MSA-EB conjugates. The binding efficiencies were determined using thin-layer chromatography. The UV-VIS spectra and molar extinction coefficient of the conjugate were obtained. The fluorescence was monitored at the excitation wavelength range 420-780 nm and the emission wavelength range 520-845 nm. The [99mTc]Tc-MSA-EB conjugate and EB were injected into the footpads of normal BALB/c mice to check the lymph node (LN) uptakes. The visible, fluorescence, and SPECT/CT images were obtained after injection. RESULTS:The conjugation of EB with MSA increased by time and was saturated within 10 min. The molar extinction coefficient of the conjugate was 99,259.3/M/cm at 620 nm. The uptake of conjugate into the popliteal LN after injection into the footpads of mice was investigated visually and fluorescence imaging. SPECT/CT images showed that the standardized uptake values of [99mTc]Tc-MSA-EB conjugate in popliteal LN were about 4 times higher than in sciatic LN at all timepoints. It was confirmed by investigating resected LN that the blue color, fluorescence, and radioactivity of the [99mTc]Tc-MSA-EB conjugate were retained only on the LN and did not spread to adjoining tissues. CONCLUSION:[99mTc]Tc-MSA-EB conjugate has a great potential as a multi-modal SLN mapping agent which could be detected by visual investigation, fluorescence imaging, and SPECT/CT.
journal_name
Nucl Med Bioljournal_title
Nuclear medicine and biologyauthors
Lee JY,Kim HY,Lee YS,Seo HY,Park JY,Jeong JMdoi
10.1016/j.nucmedbio.2020.02.002subject
Has Abstractpub_date
2020-01-01 00:00:00pages
38-45eissn
0969-8051issn
1872-9614pii
S0969-8051(19)30489-5journal_volume
84-85pub_type
杂志文章abstract::Positron emission tomography (PET), which requires a compound labeled with a positron emitter radioisotope as an imaging probe, is one of the most useful and valuable imaging modalities in molecular imaging. It has several advantages over other imaging modalities, particularly in sensitive and quantitative investigati...
journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/j.nucmedbio.2005.12.009
更新日期:2006-04-01 00:00:00
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章,评审
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更新日期:2000-10-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/0969-8051(94)00085-x
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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journal_title:Nuclear medicine and biology
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doi:10.1016/0969-8051(95)02007-1
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Nuclear medicine and biology
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/0969-8051(94)00147-c
更新日期:1995-07-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 临床试验,杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/j.nucmedbio.2007.05.010
更新日期:2007-08-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/0969-8051(94)00076-v
更新日期:1995-01-01 00:00:00
abstract:INTRODUCTION:Detection of vulnerable plaques is critically important for the selection of appropriate treatment and/or the prevention of atherosclerosis and ensuing cardiovascular diseases. In order to clarify the utility of [11C]acetate for atherosclerosis imaging, we determined the uptake and metabolism of acetate by...
journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/j.nucmedbio.2017.08.003
更新日期:2018-01-01 00:00:00
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journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/j.nucmedbio.2015.06.010
更新日期:2015-10-01 00:00:00
abstract::A new tetrahydrazido-germanium (GeTH) ligand was synthesized, characterized and complexed with 99mTc. The negatively charged 99mTc-chelate was shown to form in high yields at neutral pH in the absence of other reducing agents and exhibits high in vitro and in vivo stability. ...
journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/0969-8051(94)90184-8
更新日期:1994-11-01 00:00:00
abstract:UNLABELLED:The purpose of this study is to reveal characteristics of (64)Cu-labeled diacetyl-bis(N(4)-methylthiosemicarbazone) ([(64)Cu]Cu-ATSM) during cell proliferation and hypoxia by autoradiography imaging and immunohistochemical staining. METHODS:The intratumoral distributions of [(64)Cu]Cu-ATSM and [(18)F]-2-flu...
journal_title:Nuclear medicine and biology
pub_type: 杂志文章
doi:10.1016/j.nucmedbio.2009.01.016
更新日期:2009-05-01 00:00:00