Preparation of a multi-modal agent for sentinel lymph node mapping using Evans blue and 99mTc-labeled mannosylated human serum albumin conjugate.


INTRODUCTION:The identification of sentinel lymph nodes (SLNs) is important in deciding the resection range during surgery. 99mTc-labeled mannosylated human serum albumin ([99mTc]Tc-MSA) is a radiopharmaceutical developed for SLN detection by targeting macrophages. Evans blue (EB) is a blue dye binding strongly to albumin and has been used for SLN detection. [99mTc]Tc-MSA-EB conjugate was prepared as a multi-modal imaging agent and tested its performance by visual investigation, fluorescence imaging and SPECT/CT for SLN mapping in mice. METHODS:EB was mixed with various concentration of MSA to prepare MSA-EB conjugates. The binding efficiencies were determined using thin-layer chromatography. The UV-VIS spectra and molar extinction coefficient of the conjugate were obtained. The fluorescence was monitored at the excitation wavelength range 420-780 nm and the emission wavelength range 520-845 nm. The [99mTc]Tc-MSA-EB conjugate and EB were injected into the footpads of normal BALB/c mice to check the lymph node (LN) uptakes. The visible, fluorescence, and SPECT/CT images were obtained after injection. RESULTS:The conjugation of EB with MSA increased by time and was saturated within 10 min. The molar extinction coefficient of the conjugate was 99,259.3/M/cm at 620 nm. The uptake of conjugate into the popliteal LN after injection into the footpads of mice was investigated visually and fluorescence imaging. SPECT/CT images showed that the standardized uptake values of [99mTc]Tc-MSA-EB conjugate in popliteal LN were about 4 times higher than in sciatic LN at all timepoints. It was confirmed by investigating resected LN that the blue color, fluorescence, and radioactivity of the [99mTc]Tc-MSA-EB conjugate were retained only on the LN and did not spread to adjoining tissues. CONCLUSION:[99mTc]Tc-MSA-EB conjugate has a great potential as a multi-modal SLN mapping agent which could be detected by visual investigation, fluorescence imaging, and SPECT/CT.


Nucl Med Biol


Lee JY,Kim HY,Lee YS,Seo HY,Park JY,Jeong JM




Has Abstract


2020-01-01 00:00:00












  • Synthesis, characterization and theranostic evaluation of Indium-111 labeled multifunctional superparamagnetic iron oxide nanoparticles.

    abstract::Indium-111 labeled, Trastuzumab-Doxorubicin Conjugated, and APTES-PEG coated magnetic nanoparticles were designed for tumor targeting, drug delivery, controlled drug release, and dual-modal tumor imaging. Superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by thermal decomposition method to obtain nar...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Zolata H,Abbasi Davani F,Afarideh H

    更新日期:2015-02-01 00:00:00

  • Pretargeted tumour imaging with streptavidin immunoconjugates of monoclonal antibody CC49 and 111In-DTPA-biocytin.

    abstract::Pretargeted tumour imaging was performed in nude mice bearing subcutaneous LS174T human colon cancer xenografts with streptavidin-CC49 monoclonal antibody and 111In-DTPA-biocytin. Mice were administered 250 micrograms of streptavidin-CC49, followed 6 or 9 days later by 40 ng (250 microCi) of 111In-DTPA-biocytin. Tumor...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Alvarez-Diez TM,Polihronis J,Reilly RM

    更新日期:1996-05-01 00:00:00

  • Evaluation of nigrostriatal damage and its change over weeks in a rat model of Parkinson's disease: small animal positron emission tomography studies with [(11)C]beta-CFT.

    abstract:INTRODUCTION:The cardinal pathological feature of Parkinson's disease (PD) is progressive loss of dopaminergic neurons. Since dopamine transporter (DAT) is a protein located presynaptically on dopaminergic nerve terminals, radioligands that bind to these sites are promising radiopharmaceuticals for evaluation of the in...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Liu L,Wang Y,Li B,Jia J,Sun Z,Zhang J,Tian J,Wang X

    更新日期:2009-11-01 00:00:00

  • Synthesis, in vitro and in vivo characterization of novel 99mTc-'4+1'-labeled 5-nitroimidazole derivatives as potential agents for imaging hypoxia.

    abstract:UNLABELLED:The evaluation of oxygenation status of solid tumors is an important field of radiopharmaceutical research. With the aim to develop new potential 99mTc-radiopharmaceuticals for imaging hypoxia, we have synthesized two novel isocyanide derivatives of metronidazole, which has demonstrated high affinity for hyp...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Giglio J,Fernández S,Pietzsch HJ,Dematteis S,Moreno M,Pacheco JP,Cerecetto H,Rey A

    更新日期:2012-07-01 00:00:00

  • Determination of the target volume of HeLa cells treated with platinum-195m radiolabeled trans-diaminedichloroplatinum(II): a comparison with cis-diaminedichloroplatinum(II).

    abstract::HeLa S-3 cells were treated with 195mPt-radiolabeled trans-diaminedichloroplatinum(II) (TDDP) under various conditions, and the relationship between lethal effect and the number of Pt atoms binding to DNA, RNA and proteins was examined. The mean lethal concentrations for the cells treated with TDDP at 37 degrees C for...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Akaboshi M,Kawai K,Maki H,Akuta K,Ujeno Y,Ono K,Miyahara T

    更新日期:1993-05-01 00:00:00

  • Electrophilic 18F from a Siemens 11 MeV proton-only cyclotron.

    abstract::Because more and more PET centres are using small proton cyclotrons there is a renewed interest in methods for the production of electrophilic 18F by proton irradiation of [18O]O2. A method for the routine production of clinically useful quantities of [18F]F2 having a specific activity of 35 Ci/mmol has been developed...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Chirakal R,Adams RM,Firnau G,Schrobilgen GJ,Coates G,Garnett ES

    更新日期:1995-01-01 00:00:00

  • Sequence-specific binding and cleavage of duplex DNA by a radioiodinated, intercalator-linked, triplex-forming oligonucleotide.

    abstract::Applications of oligodeoxynucleotides to modulate gene expression have been the subject of much recent research. We have sought to develop a method to permanently inactivate a gene, or potentially kill cells containing abnormal genes. In this report, we show that a DNA intercalator conjugated to a triplex-forming olig...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Orson FM,McShan WM,Kinsey BM

    更新日期:1996-05-01 00:00:00

  • Drugs interacting with organic anion transporter-1 affect uptake of Tc-99m-mercaptoacetyl-triglycine (MAG3) in the human kidney: therapeutic drug interaction in Tc-99m-MAG3 diagnosis of renal function and possible application of Tc-99m-MAG3 for drug devel

    abstract:INTRODUCTION:Renal uptake of Tc-99m-MG3 involves organic anion transporter (OAT). Treatment with drugs showing OAT affinity might interfere with renal uptake of Tc-99m-MAG3, leading to misinterpretation in Tc-99m-MAG3. This study was conducted to discuss a possible drug interference with Tc-99m-MAG3 diagnosis on OAT si...

    journal_title:Nuclear medicine and biology

    pub_type: 临床试验,杂志文章


    authors: Takahara N,Saga T,Inubushi M,Kusuhara H,Seki C,Ito S,Oyama N,Yokoyama O,Sugiyama Y,Fujibayashi Y

    更新日期:2013-07-01 00:00:00

  • Amino acid PET tracers are reliable markers of treatment responses to single-agent or combination therapies including temozolomide, interferon-β, and/or bevacizumab for glioblastoma.

    abstract:INTRODUCTION:We examined whether the amino acid PET tracers, trans-1-amino-3-(18)F-fluorocyclobutanecarboxylic acid (anti-(18)F-FACBC) and (11)C-methyl-l-methionine ((11)C-Met), are suitable for detecting early responses to combination therapies including temozolomide (TMZ), interferon-β (IFN), and bevacizumab (Bev) in...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ono T,Sasajima T,Doi Y,Oka S,Ono M,Kanagawa M,Baden A,Mizoi K,Shimizu H

    更新日期:2015-07-01 00:00:00

  • Potent candidates for Targeted Auger Therapy: Production and radiochemical considerations.

    abstract::Targeted Auger Therapy represents great potential for the therapy of diseases which require a high degree of selectivity on the cellular level (e.g. for therapy of metastatic cancers). Due to their high Linear Energy Transfer (LET), Auger emitters, combined with selective biological systems which enable delivery of ra...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章,评审


    authors: Filosofov D,Kurakina E,Radchenko V

    更新日期:2020-12-13 00:00:00

  • Radiosynthesis of [11C]brofaromine, a potential tracer for imaging monoamine oxidase A.

    abstract::Brofaromine (4-5(-methoxy-7-bromobenzofuranyl)-2-piperidine-HCl) is a potent and selective inhibitor of monoamine oxidase (MAO) A. Two methods for its synthesis and a preliminary positron emission tomography (PET) evaluation in monkey brain are described. The first method, at low carrier concentration of CO2, consiste...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ametamey SM,Beer HF,Guenther I,Antonini A,Leenders KL,Waldmeier PC,Schubiger PA

    更新日期:1996-04-01 00:00:00

  • In vitro uptake and metabolism of [14C]acetate in rabbit atherosclerotic arteries: biological basis for atherosclerosis imaging with [11C]acetate.

    abstract:INTRODUCTION:Detection of vulnerable plaques is critically important for the selection of appropriate treatment and/or the prevention of atherosclerosis and ensuing cardiovascular diseases. In order to clarify the utility of [11C]acetate for atherosclerosis imaging, we determined the uptake and metabolism of acetate by...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Yamasaki K,Yamashita A,Zhao Y,Shimizu Y,Nishii R,Kawai K,Tamaki N,Zhao S,Asada Y,Kuge Y

    更新日期:2018-01-01 00:00:00

  • Evaluation of in vivo selective binding of [11C]doxepin to histamine H1 receptors in five animal species.

    abstract::The specific binding of [(11)C]doxepin, which has been used as a radioligand for mapping histamine H(1) receptors in human brain by positron emission tomography, was evaluated in five animal species. In mice the [(11)C]doxepin uptake was reduced by treatment with cold doxepin and two H(1) receptor antagonists, but not...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ishiwata K,Kawamura K,Wang WF,Tsukada H,Harada N,Mochizuki H,Kimura Y,Ishii K,Iwata R,Yanai K

    更新日期:2004-05-01 00:00:00

  • In vitro metabolism studies of (18)F-labeled 1-phenylpiperazine using mouse liver S9 fraction.

    abstract::The in vitro metabolism of 1-(4-[(18)F]fluoromethylbenzyl)-4-phenylpiperazine ([(18)F]1) and 1-(4-[(18)F]fluorobenzyl)-4-phenylpiperazine ([(18)F]2) was investigated using mouse liver S9 fraction. Results were compared to those of in vivo metabolism using mouse blood and bone and to in vitro metabolism using mouse liv...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ryu EK,Choe YS,Kim DH,Ko BH,Choi Y,Lee KH,Kim BT

    更新日期:2006-02-01 00:00:00

  • Compartmental analysis of the pharmacokinetics of radioiodinated monoclonal antibody B72.3 in colon cancer patients.

    abstract::Sixteen patients with colorectal cancer were administered 37-74 MBq (1 mg) of radioiodinated B72.3 monoclonal antibody. Pharmacokinetic analysis was carried out on plasma and urine samples. Elimination from the plasma was biexponential with a mean T1/2 alpha of 3.7 h and T1/2 beta of 62.4 h. The plasma clearance was f...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Reilly RM,Kirsh J,Gallinger S,Thiessen JJ,Damani M,Hay K,Polihronis J,Schmocker B,Odze R,Houle S

    更新日期:1993-01-01 00:00:00

  • Determination of specific radioactivity for (76)Br-labeled compounds measuring the ratio between (76)Br and (79)Br using packed capillary liquid chromatography mass spectrometry.

    abstract::Packed capillary liquid chromatography with electrospray mass spectrometry was used for direct determination of the specific radioactivity by calculation of isotope ratios between the (76)Br- and (79)Br-labeled analogues of N-((3-aminomethyl)benzyl)-4-bromobenzamide. Using 20 microL injections on packed capillary colu...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Forngren BH,Yngve U,Forngren T,Långström B

    更新日期:2000-11-01 00:00:00

  • Intraarticular and intravenous administration of 99MTc-HMPAO-labeled human mesenchymal stem cells (99MTC-AH-MSCS): In vivo imaging and biodistribution.

    abstract:INTRODUCTION:Therapeutic application of intravenous administered (IV) human bone marrow-derived mesenchymal stem cells (ahMSCs) appears to have as main drawback the massive retention of cells in the lung parenchyma, questioning the suitability of this via of administration. Intraarticular administration (IAR) could be ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Meseguer-Olmo L,Montellano AJ,Martínez T,Martínez CM,Revilla-Nuin B,Roldán M,Mora CF,López-Lucas MD,Fuente T

    更新日期:2017-03-01 00:00:00

  • System a amino acid transport-targeted brain and systemic tumor PET imaging agents 2-amino-3-[(18)F]fluoro-2-methylpropanoic acid and 3-[(18)F]fluoro-2-methyl-2-(methylamino)propanoic acid.

    abstract:INTRODUCTION:Amino acid based radiotracers target tumor cells through increased uptake by membrane-associated amino acid transport (AAT) systems. In the present study, four structurally related non-natural (18)F-labeled amino acids, (R)- and (S)-[(18)F]FAMP 1 and (R)- and (S)-[(18)F]MeFAMP 2 have been prepared and eval...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Yu W,McConathy J,Olson JJ,Goodman MM

    更新日期:2015-01-01 00:00:00

  • Production of [11C]CH3I by single pass reaction of [11C]CH4 with I2.

    abstract::We report a single pass gas phase route to [11C]CH3I based on production of I from I2 at elevated temperature. I abstracts H from CH4 to yield CH3, which reacts with I2 to form CH3I. A fiber optic spectrometer was used to measure the I2 vapor concentration. [11C]CH3I yield increased with I2 concentration until excessi...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Link JM,Krohn KA,Clark JC

    更新日期:1997-01-01 00:00:00

  • Exploration of the impact of stereochemistry on the identification of the novel translocator protein PET imaging agent [(18)F]GE-180.

    abstract:INTRODUCTION:The tricyclic indole compound, [(18)F]GE-180 has been previously identified as a promising positron emission tomography (PET) imaging agent of the translocator protein (TSPO) with the potential to aid in the diagnosis, prognosis and therapy monitoring of degenerative neuroinflammatory conditions such as mu...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Chau WF,Black AM,Clarke A,Durrant C,Gausemel I,Khan I,Mantzilas D,Oulie I,Rogstad A,Trigg W,Jones PA

    更新日期:2015-09-01 00:00:00

  • The chemical nature, purity and stability of 99mTc-N-(2,6-Diethyl-3-iodo-phenylcarbamoylmethyl)-iminodiacetic acid.

    abstract::The chemical purity and stability of a radio pharmaceutical 99mTc-N-(2,6-diethyl-3-iodo-phenylcarbamoylmethyl)-iminodiacetic acid (IODIDA) has been studied using HPLC. The HPLC results indicate that IODIDA decomposed into at least three species after 99mTc labelling, and the amount of these varied with time. Mass spec...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Lundberg PS,Jacobsson L,Stenhagen G,Mårtensson J,Fjälling M

    更新日期:1995-05-01 00:00:00

  • Preclinical evaluation of new α-radionuclide therapy targeting LAT1: 2-[211At]astato-α-methyl-L-phenylalanine in tumor-bearing model.

    abstract:INTRODUCTION:Targeted α-radionuclide therapy has attracted attention as a promising therapy for refractory cancers. However, the application is limited to certain types of cancer. Since L-type amino acid transporter 1 (LAT1) is highly expressed in various human cancers, we prepared an LAT1-selective α-radionuclide-labe...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ohshima Y,Suzuki H,Hanaoka H,Sasaki I,Watanabe S,Haba H,Arano Y,Tsushima Y,Ishioka NS

    更新日期:2020-01-01 00:00:00

  • Evaluation of a chloride-based 89Zr isolation strategy using a tributyl phosphate (TBP)-functionalized extraction resin.

    abstract:INTRODUCTION:The remarkable stability of the 89Zr-DOTA complex has been shown in recent literature. The formation of this complex appears to require 89Zr-chloride as the complexation precursor rather than the more conventional 89Zr-oxalate. In this work we present a method for the direct isolation of 89Zr-chloride from...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Graves SA,Kutyreff C,Barrett KE,Hernandez R,Ellison PA,Happel S,Aluicio-Sarduy E,Barnhart TE,Nickles RJ,Engle JW

    更新日期:2018-01-01 00:00:00

  • PET imaging of TSPO in a rat model of local neuroinflammation induced by intracerebral injection of lipopolysaccharide.

    abstract:OBJECTIVE:The goal of this study was to measure functional and structural aspects of local neuroinflammation induced by intracerebral injection of lipopolysaccharide (LPS) in rats using TSPO microPET imaging with [(18)F]DPA-714, magnetic resonance imaging (MRI), in vitro autoradiography and immunohistochemistry (IHC) i...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Ory D,Planas A,Dresselaers T,Gsell W,Postnov A,Celen S,Casteels C,Himmelreich U,Debyser Z,Van Laere K,Verbruggen A,Bormans G

    更新日期:2015-10-01 00:00:00

  • Biodistribution studies of two 18F-labeled pyridinylphenyl amides as subtype selective radioligands for the dopamine D3 receptor.

    abstract:INTRODUCTION:Dopamine D3 receptors are implicated in various neuropsychiatric diseases, drug abuse and alcoholism, but specific agents for D3 molecular imaging are lacking. We evaluated two in vitro selective fluorine-18-labeled radioligand candidates ([(18)F]5 and [(18)F]6) for positron emission tomography (PET) imagi...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Hocke C,Cumming P,Maschauer S,Kuwert T,Gmeiner P,Prante O

    更新日期:2014-03-01 00:00:00

  • Synthesis of fluorine-18-labeled ciprofloxacin for PET studies in humans.

    abstract::Ciprofloxacin (1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-quinoline-3-carboxylic acid), a widely-prescribed antibiotic, was labeled with fluorine-18 with the aim to perform positron emission tomography studies in humans for pharmacokinetic measurements. Due to a lack of chemical activation of ciproflox...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Langer O,Mitterhauser M,Brunner M,Zeitlinger M,Wadsak W,Mayer BX,Kletter K,Müller M

    更新日期:2003-04-01 00:00:00

  • Positron emission tomography in drug evaluation: influence of three different catechol-O-methyltransferase inhibitors on metabolism of [NCA] 6-[18F]fluoro-L-dopa in rhesus monkey.

    abstract::We compared the influence of three different catechol-O-methyltransferase (COMT) inhibitors (CGP 28014, OR-611 and Ro 40-7592) on the metabolism of no-carrier-added (NCA) 6-[18F]fluoro-L-dopa (6-FDOPA) in one Rhesus monkey. All three COMT inhibitors improved 6-FDOPA availability in plasma, increased the specific uptak...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Günther I,Psylla M,Reddy GN,Antonini A,Vontobel P,Reist HW,Zollinger A,Nickles RJ,Beer HF,Schubiger PA

    更新日期:1995-10-01 00:00:00

  • Improving the quantitation accuracy in noninvasive small animal single photon emission computed tomography imaging.

    abstract:INTRODUCTION:Noninvasive imaging of small animals to measure biodistributions and pharmacokinetics of radiolabeled agents is increasingly seen as an effective alternative to external counting of tissues obtained by sacrifice and dissection. However, we have observed important disagreements in measuring the accumulation...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Cheng D,Rusckowski M,Pretorius PH,Chen L,Xiao N,Liu Y,Liu G,Liang M,Liu X,Dou S,Hnatowich DJ

    更新日期:2011-08-01 00:00:00

  • Pharmacokinetics of the thymidine analog 2'-fluoro-5-methyl-1-beta-D-arabinofuranosyluracil (FMAU) in tumor-bearing rats.

    abstract::The thymidine analog 2'-fluoro-5-methyl-1-beta-D-arabinofuranosyluracil (FMAU) is incorporated into DNA and is resistant to catabolism. We performed pharmacokinetic measurements with [(14)C]FMAU and PET studies with [(11)C]FMAU using rats bearing several different syngeneic tumors. Among normal tissues, FMAU uptake re...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Bading JR,Shahinian AH,Vail A,Bathija P,Koszalka GW,Koda RT,Alauddin MM,Fissekis JD,Conti PS

    更新日期:2004-05-01 00:00:00

  • Preliminary assessment of extrastriatal dopamine D-2 receptor binding in the rodent and nonhuman primate brains using the high affinity radioligand, 18F-fallypride.

    abstract::We have identified the value of 18F-fallypride [(S)-N-[(1-allyl-2-pyrrolidinyl)methyl]-5-(3-[18F]fluoropropyl)-2, 3-dimethoxybenzamide], as a dopamine D-2 receptor radiotracer for the study of striatal and extrastriatal receptors. Fallypride exhibits high affinities for D-2 and D-3 subtypes and low affinity for D-4 (3...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Mukherjee J,Yang ZY,Brown T,Lew R,Wernick M,Ouyang X,Yasillo N,Chen CT,Mintzer R,Cooper M

    更新日期:1999-07-01 00:00:00