Preparation of a multi-modal agent for sentinel lymph node mapping using Evans blue and 99mTc-labeled mannosylated human serum albumin conjugate.


INTRODUCTION:The identification of sentinel lymph nodes (SLNs) is important in deciding the resection range during surgery. 99mTc-labeled mannosylated human serum albumin ([99mTc]Tc-MSA) is a radiopharmaceutical developed for SLN detection by targeting macrophages. Evans blue (EB) is a blue dye binding strongly to albumin and has been used for SLN detection. [99mTc]Tc-MSA-EB conjugate was prepared as a multi-modal imaging agent and tested its performance by visual investigation, fluorescence imaging and SPECT/CT for SLN mapping in mice. METHODS:EB was mixed with various concentration of MSA to prepare MSA-EB conjugates. The binding efficiencies were determined using thin-layer chromatography. The UV-VIS spectra and molar extinction coefficient of the conjugate were obtained. The fluorescence was monitored at the excitation wavelength range 420-780 nm and the emission wavelength range 520-845 nm. The [99mTc]Tc-MSA-EB conjugate and EB were injected into the footpads of normal BALB/c mice to check the lymph node (LN) uptakes. The visible, fluorescence, and SPECT/CT images were obtained after injection. RESULTS:The conjugation of EB with MSA increased by time and was saturated within 10 min. The molar extinction coefficient of the conjugate was 99,259.3/M/cm at 620 nm. The uptake of conjugate into the popliteal LN after injection into the footpads of mice was investigated visually and fluorescence imaging. SPECT/CT images showed that the standardized uptake values of [99mTc]Tc-MSA-EB conjugate in popliteal LN were about 4 times higher than in sciatic LN at all timepoints. It was confirmed by investigating resected LN that the blue color, fluorescence, and radioactivity of the [99mTc]Tc-MSA-EB conjugate were retained only on the LN and did not spread to adjoining tissues. CONCLUSION:[99mTc]Tc-MSA-EB conjugate has a great potential as a multi-modal SLN mapping agent which could be detected by visual investigation, fluorescence imaging, and SPECT/CT.


Nucl Med Biol


Lee JY,Kim HY,Lee YS,Seo HY,Park JY,Jeong JM




Has Abstract


2020-01-01 00:00:00












  • Synthesis and characterization of lanthanum phosphate nanoparticles as carriers for (223)Ra and (225)Ra for targeted alpha therapy.

    abstract:INTRODUCTION:Targeted alpha therapy (TAT) has the potential for killing micro-metastases with minimum collateral damage to surrounding healthy tissue. In-vivo generator radionuclides, such as(223)Ra, (225)Ra, and (225)Ac, are of special interest for radiotherapeutic applications as they emit multiple α-particles during...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Rojas JV,Woodward JD,Chen N,Rondinone AJ,Castano CH,Mirzadeh S

    更新日期:2015-07-01 00:00:00

  • In vivo characterization of radioiodinated (+)-2-[4-(4-iodophenyl) piperidino] cyclohexanol as a potential sigma-1 receptor imaging agent.

    abstract::In this study, the (+)-enantiomer of radioiodinated 2-[4-(4-iodophenyl)piperidino]cyclohexanol [(+)-[(125)I]-p-iodovesamicol] [(+)-[(125)I]pIV], which is reported to bind with high affinity to sigma-1 receptors in vitro, was tested for its usefulness in imaging sigma-1 receptors in the central nervous system (CNS) in ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Akhter N,Shiba K,Ogawa K,Kinuya S,Nakajima K,Mori H

    更新日期:2007-08-01 00:00:00

  • Column-switching HPLC for the analysis of plasma in PET imaging studies.

    abstract::A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [(11)C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Hilton J,Yokoi F,Dannals RF,Ravert HT,Szabo Z,Wong DF

    更新日期:2000-08-01 00:00:00

  • Modulation of organ uptake of 11C-labelled L-DOPA.

    abstract::The present study was undertaken to investigate if pretreatment with pharmacological agents could change the organ uptake of 11C-labelled L-DOPA, and especially if the urinary excretion could be decreased. L-[beta-11C]DOPA was injected IV into unanesthetized Sprague-Dawley rats. After 20 min the rats were decapitated ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Bergström M,Lu L,Marquez M,Fasth KJ,Bjurling P,Watanabe Y,Eriksson B,Långström B

    更新日期:1997-01-01 00:00:00

  • Evaluation of [18F]Nifene biodistribution and dosimetry based on whole-body PET imaging of mice.

    abstract:INTRODUCTION:[(18)F]Nifene is a novel radiotracer specific to the nicotinic acetylcholine α4β2 receptor class. In preparation for using this tracer in humans we have performed whole-body PET studies in mice to evaluate the in vivo biodistribution and dosimetry of [(18)F]Nifene. METHODS:Seven BALB/c mice (3 males, 4 fe...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Constantinescu CC,Garcia A,Mirbolooki MR,Pan ML,Mukherjee J

    更新日期:2013-02-01 00:00:00

  • Imaging recognition of multidrug resistance in human breast tumors using 99mTc-labeled monocationic agents and a high-resolution stationary SPECT system.

    abstract::Imaging recognition of multidrug-resistance by 99mTc-labeled sestamibi, tetrofosmin and furifosmin in mice bearing human breast tumors was evaluated using a high-resolution SPECT, FASTSPECT. Imaging results showed that the washout rates in drug-resistant MCF7/D40 tumors were significantly greater than that in drug-sen...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Liu Z,Stevenson GD,Barrett HH,Kastis GA,Bettan M,Furenlid LR,Wilson DW,Woolfenden JM

    更新日期:2004-01-01 00:00:00

  • Functional neuroanatomy of the basal ganglia as studied by dual-probe microdialysis.

    abstract::Dual probe microdialysis was employed in intact rat brain to investigate the effect of intrastriatal perfusion with selective dopamine D1 and D2 receptor agonists and with c-fos antisense oligonucleotide on (a) local GABA release in the striatum; (b) the internal segment of the globus pallidus and the substantia nigra...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: O'Connor WT

    更新日期:1998-11-01 00:00:00

  • An alumina ceramic target vessel for the remote production of metallic radionuclides by in situ target dissolution.

    abstract:INTRODUCTION:As the use of metallic radionuclides increases, so does the demand for a simple production method. In this study, we demonstrated an in situ target processing concept for automated metallic radionuclide production without the use of any robotic device. METHODS:An alumina ceramic vessel for a vertical irra...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Nagatsu K,Suzuki H,Fukada M,Minegishi K,Tsuji A,Fukumura T

    更新日期:2012-11-01 00:00:00

  • Whole-body distribution and radiation dosimetry of [11C]telmisartan as a biomarker for hepatic organic anion transporting polypeptide (OATP) 1B3.

    abstract:INTRODUCTION:Telmisartan, a nonpeptide angiotensin II AT1 receptor antagonist used as an antihypertensive drug, is specifically taken up by the liver through the OATP1B3. PET imaging with [(11)C]telmisartan is expected to provide information about the whole body pharmacokinetics of telmisartan as well as its transport ...

    journal_title:Nuclear medicine and biology

    pub_type: 临床试验,杂志文章


    authors: Shimizu K,Takashima T,Yamane T,Sasaki M,Kageyama H,Hashizume Y,Maeda K,Sugiyama Y,Watanabe Y,Senda M

    更新日期:2012-08-01 00:00:00

  • Synthesis and preliminary evaluation of 9-(4-[18F]-fluoro-3-hydroxymethylbutyl)guanine ([18F]FHBG): a new potential imaging agent for viral infection and gene therapy using PET.

    abstract::Synthesis and preliminary biological evaluation of 9-(4-[18F]-fluoro-3-hydroxymethylbutyl)-guanine ([18F]FHBG) is reported. 9-(4-Hydroxy-3-hydroxymethylbutyl)-guanine (penciclovir) 4 was converted to 9-[N2, O-bis-(methoxytrityl)-3-(tosylmethybutyl)]guanine 7 by treatment with methoxytrityl chloride followed by tosylat...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Alauddin MM,Conti PS

    更新日期:1998-04-01 00:00:00

  • Distribution of carbon-11 labeled methamphetamine and the effect of its chronic administration in mice.

    abstract::[11C]Methamphetamine, a psychotropic agent, was synthesized by N-methylation of amphetamine with [11C]CH3 I in hopes that it could be applied in the near future to assist positron emission tomography (PET) in the imaging of its distribution in the human brain. The regional distribution of [11C]methamphetamine was inve...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Mizugaki M,Hishinuma T,Nakamura H,Yamashita M,Shimomura K,Itoh K,Hirose A,Tomioka Y,Aso H,Edo K

    更新日期:1993-05-01 00:00:00

  • In vivo inhibition of neutral endopeptidase enhances the diagnostic potential of truncated gastrin (111)In-radioligands.

    abstract:INTRODUCTION:Radiolabeled gastrin analogs represent attractive candidates for diagnosis and therapy of cholecystokinin subtype-2 receptor (CCK2R)-expressing tumors. Radiolabeled des(Glu)5-gastrins show favorably low renal accumulation, but localize poorly in CCK2R-positive lesions. We introduce herein three truncated [...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Kaloudi A,Nock BA,Lymperis E,Sallegger W,Krenning EP,de Jong M,Maina T

    更新日期:2015-11-01 00:00:00

  • N1'-fluoroethyl-naltrindole (BU97001) and N1'-fluoroethyl-(14-formylamino)-naltrindole (BU97018) potential delta-opioid receptor PET ligands.

    abstract::The properties of two prospective positron emission tomography (PET) ligands for the delta-opioid receptor, N1'-fluoroethyl-naltrindole (BU97001) and N1'-fluoroethyl-(14-formylamino)-naltrindole (BU97018) were investigated. Both were antagonists in the mouse vas deferens, and showed high affinity and selectivity, 1.81...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Tyacke RJ,Robinson ES,Schnabel R,Lewis JW,Husbands SM,Nutt DJ,Hudson AL

    更新日期:2002-05-01 00:00:00

  • Synthesis and in vivo brain distribution of carbon-11-labeled δ-opioid receptor agonists.

    abstract::Three new radiolabeled compounds, [(11)C]SNC80 ((+)-4-[(αR)-α-{(2S,5R)-4-allyl-2,5-dimethyl-1-piperazinyl}-3-[(11)C]methoxybenzyl-N,N-diethylbenzamide), N,N-diethyl-4-[3-methoxyphenyl-1-[(11)C]methylpiperidin-4-ylidenemethyl)benzamide and N,N-diethyl-4-[(1-[(11)C]methylpiperidin-4-ylidene)phenylmethyl]benzamide, were ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Pichika R,Jewett DM,Sherman PS,Traynor JR,Husbands SM,Woods JH,Kilbourn MR

    更新日期:2010-11-01 00:00:00

  • Kinetics of the metabolism of four PET radioligands in living minipigs.

    abstract::Most radioligands are substantially metabolised in peripheral organs during the course of positron emission tomography (PET) recordings. Accurate determination of plasma concentrations of unmetabolised radioligands is often important for quantification of data from PET studies. The fractions of untransformed radioliga...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Gillings NM,Bender D,Falborg L,Marthi K,Munk OL,Cumming P

    更新日期:2001-01-01 00:00:00

  • A new nucleophilic fluorine-18 labeling method for aliphatic mesylates: reaction in ionic liquids shows tolerance for water.

    abstract::[see text for equation] Nucleophilic [(18)F]fluorination of some halo- and mesyloxyalkanes to the corresponding [(18)F]fluoroalkanes with fluoride-18 obtained from an (18)O(p,n) [(18)F]F reaction, using an ionic liquid as a reaction medium, has been studied as a new method for fluorine-18 labeling. Of the various ioni...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Kim DW,Choe YS,Chi DY

    更新日期:2003-05-01 00:00:00

  • A strategy for increasing the brain uptake of a radioligand in animals: use of a drug that inhibits plasma protein binding.

    abstract::A positron-emitter labeled radioligand for the glycine-binding site of the N-methyl-D-aspartate (NMDA) receptor, [(11)C]L-703,717, was examined for its ability to penetrate the brain in animals by simultaneous use with drugs having high-affinity separate binding sites on human serum albumin. [(11)C]L-703,717 has poor ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Haradahira T,Zhang M,Maeda J,Okauchi T,Kawabe K,Kida T,Suzuki K,Suhara1 T

    更新日期:2000-05-01 00:00:00

  • [11C]-MeJDTic: a novel radioligand for kappa-opioid receptor positron emission tomography imaging.

    abstract:INTRODUCTION:Radiopharmaceuticals that can bind selectively the kappa-opioid receptor may present opportunities for staging clinical brain disorders and evaluating the efficiency of new therapies related to stroke, neurodegenerative diseases or opiate addiction. The N-methylated derivative of JDTic (named MeJDTic), whi...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Poisnel G,Oueslati F,Dhilly M,Delamare J,Perrio C,Debruyne D,Barré L

    更新日期:2008-07-01 00:00:00

  • Uptake of 99mTc-labeled chondroitin sulfate by chondrocytes and cartilage: a promising agent for imaging of cartilage degeneration?

    abstract::Chondroitin sulfate (CS) is used in the treatment of human osteoarthritis as a slow-acting symptomatic drug. For this reason, we performed uptake studies with (99m)TcCS using different chondrocyte cultures, as well as cartilage tissue in vitro. For uptake studies, adherent monolayer cultures of human chondrocytes (2.7...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Sobal G,Menzel J,Sinzinger H

    更新日期:2009-01-01 00:00:00

  • Synthesis of O-(2-[18F]fluoroethyl)-L-tyrosine based on a cartridge purification method.

    abstract:INTRODUCTION:O-(2-[(18)F]fluoroethyl)-L-tyrosine (FET) is widely used as a positron emission tomography tracer for brain tumors. Usually, a high-performance liquid chromatography (HPLC) purification at the end of the two-step synthesis is applied. In this work, we report an automatic radiosynthesis of FET with a purifi...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Mueller D,Klette I,Kalb F,Baum RP

    更新日期:2011-07-01 00:00:00

  • The synthesis, magnetic purification and evaluation of 99mTc-labeled microbubbles.

    abstract:INTRODUCTION:Ultrasound (US) contrast agents based on microbubbles (MBs) are being investigated as platforms for drug and gene delivery. A methodology for determining the distribution and fate of modified MBs quantitatively in vivo can be achieved by tagging MBs directly with (99m)Tc. This creates the opportunity to em...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Lazarova N,Causey PW,Lemon JA,Czorny SK,Forbes JR,Zlitni A,Genady A,Foster FS,Valliant JF

    更新日期:2011-11-01 00:00:00

  • Electrophilic 18F from a Siemens 11 MeV proton-only cyclotron.

    abstract::Because more and more PET centres are using small proton cyclotrons there is a renewed interest in methods for the production of electrophilic 18F by proton irradiation of [18O]O2. A method for the routine production of clinically useful quantities of [18F]F2 having a specific activity of 35 Ci/mmol has been developed...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Chirakal R,Adams RM,Firnau G,Schrobilgen GJ,Coates G,Garnett ES

    更新日期:1995-01-01 00:00:00

  • Preparation and pharmacokinetics of 11C labeled stavudine (d4T).

    abstract::Stavudine, a potent antiviral agent for treating human immunodeficiency virus (HIV) infections, was radiolabeled with (11)C by methylation of a specifically designed precursor, 5'-O-(2-tetrahydropyranyl)-5-bromo-2',3'-didehydro-3'-deoxythymidine, with (11)C H(3)I. The radiolabeled drug was isolated by reverse phase HP...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Livni E,Berker M,Hillier S,Waller SC,Ogan MD,Discordia RP,Rienhart JK,Rubin RH,Fischman AJ

    更新日期:2004-07-01 00:00:00

  • Potent candidates for Targeted Auger Therapy: Production and radiochemical considerations.

    abstract::Targeted Auger Therapy represents great potential for the therapy of diseases which require a high degree of selectivity on the cellular level (e.g. for therapy of metastatic cancers). Due to their high Linear Energy Transfer (LET), Auger emitters, combined with selective biological systems which enable delivery of ra...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章,评审


    authors: Filosofov D,Kurakina E,Radchenko V

    更新日期:2020-12-13 00:00:00

  • [(11)]Cocaine: PET studies of cocaine pharmacokinetics, dopamine transporter availability and dopamine transporter occupancy.

    abstract::Cocaine was initially labeled with carbon-11 in order to track the distribution and pharmacokinetics of this powerful stimulant and drug of abuse in the human brain and body. It was soon discovered that [(11)C]cocaine was not only useful for measuring cocaine pharmacokinetics and its relationship to behavior but that ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章,评审


    authors: Fowler JS,Volkow ND,Wang GJ,Gatley SJ,Logan J

    更新日期:2001-07-01 00:00:00

  • Synthesis and in vivo evaluation of [O-methyl-11C](2R,4R)-4-hydroxy-2-[2-[2-[2-(3-methoxy)phenyl]ethyl]phenoxy]ethyl-1-methylpyrrolidine as a 5-HT2A receptor PET ligand.

    abstract::The serotonin2A (5-HT2A) receptor is implicated in the pathophysiology of schizophrenia and mood disorders, and in vivo studies of this receptor would be of value in studying the pathophysiology of these disorders and in measuring the relationship of clinical response to receptor occupancy for 5-HT2A antagonists such ...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Kumar JS,Prabhakaran J,Erlandsson K,Majo VJ,Simpson NR,Pratap M,Van Heertum RL,Mann JJ,Parsey RV

    更新日期:2006-05-01 00:00:00

  • Chemical reactivity of the 18F electrophilic reagents from the 18O(p,n) 18F gas target systems.

    abstract::A comprehensive evaluation of the reactivity of 18F electrophilic fluorinating agents derived from the 18O(p,n)18F reaction conducted in target bodies made of aluminum, silver, copper, nickel, and gold-plated copper is reported. Two representative electrophilic reactions, namely addition across a double bond and subst...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Bishop A,Satyamurthy N,Bida G,Barrio JR

    更新日期:1996-07-01 00:00:00

  • Fluorine-18 labeled diphenyl sulfide derivatives for imaging serotonin transporter (SERT) in the brain.

    abstract:OBJECTIVES:Serotonin transporters (SERT) play an important role in controlling serotonin concentration in the synaptic cleft and in managing postsynaptic signal transduction. Inhibitors of SERT binding are well known as selective serotonin reuptake inhibitors (SSRIs) such as fluoxetine, sertraline, paroxetine, and esci...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Zhang Y,Liu F,Xiao H,Yao X,Li G,Choi SR,Ploessl K,Zha Z,Zhu L,Kung HF

    更新日期:2018-11-01 00:00:00

  • Evaluation of a chloride-based 89Zr isolation strategy using a tributyl phosphate (TBP)-functionalized extraction resin.

    abstract:INTRODUCTION:The remarkable stability of the 89Zr-DOTA complex has been shown in recent literature. The formation of this complex appears to require 89Zr-chloride as the complexation precursor rather than the more conventional 89Zr-oxalate. In this work we present a method for the direct isolation of 89Zr-chloride from...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Graves SA,Kutyreff C,Barrett KE,Hernandez R,Ellison PA,Happel S,Aluicio-Sarduy E,Barnhart TE,Nickles RJ,Engle JW

    更新日期:2018-01-01 00:00:00

  • G2M arrest and apoptosis in murine T lymphoma cells following exposure to 212Bi alpha particle irradiation.

    abstract::Asynchronous exponentially growing EL4 murine T lymphoma cells were exposed either to high LET alpha-radiation from 212Bi-DTPA or to gamma-radiation from a 137Cs source. Radiation-induced cell cycle perturbation was studied by flow cytometry. Alpha irradiation, like gamma, transiently arrested cells in the G2M phase i...

    journal_title:Nuclear medicine and biology

    pub_type: 杂志文章


    authors: Palayoor ST,Humm JL,Atcher RW,Hines JJ,Macklis RM

    更新日期:1993-08-01 00:00:00