Structural analysis of a trimeric assembly of the mitochondrial dynamin-like GTPase Mgm1.

abstract：:The use of molecular methods is altering our understanding of the microbial biosphere and the complexity of the tree of life. Here, we report a newly discovered uncultured plastid-bearing eukaryotic lineage named the rappemonads. Phylogenies using near-complete plastid ribosomal DNA (rDNA) operons demonstrate that thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim E,Harrison JW,Sudek S,Jones MD,Wilcox HM,Richards TA,Worden AZ,Archibald JM

更新日期：2011-01-25 00:00:00

abstract：:In an unprecedented finding, Davis et al. [Davis, R. E., Miller, S., Herrnstadt, C., Ghosh, S. S., Fahy, E., Shinobu, L. A., Galasko, D., Thal, L. J., Beal, M. F., Howell, N. & Parker, W. D., Jr. (1997) Proc. Natl. Acad. Sci. USA 94, 4526-4531] used an unusual DNA isolation method to show that healthy adults harbor a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirano M,Shtilbans A,Mayeux R,Davidson MM,DiMauro S,Knowles JA,Schon EA

更新日期：1997-12-23 00:00:00

abstract：:The genetic coupling hypothesis of signal-preference evolution, whereby the same genes control male signal and female preference for that signal, was first inspired by the evolution of cricket acoustic communication nearly 50 years ago. To examine this hypothesis, we compared the genomic location of quantitative trait...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaw KL,Lesnick SC

更新日期：2009-06-16 00:00:00

abstract：:Exposure of receptor-rich membrane fragments from Torpedo marmorata to carbamylcholine causes a slow (half-time of 5--10 min) and reversible change of properties of the cholinergic receptor protein manifested by a decrease of the initial rate of Naja nigricollis alpha-[3H]toxin binding in the presence of carbamylcholi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weber M,David-Pfeuty T,Changeux JP

更新日期：1975-09-01 00:00:00

abstract：:During reverse transcription of retroviral RNA, synthesis of (-) strand DNA is primed by a cellular tRNA that anneals to an 18-nt primer binding site within the 5' long terminal repeat. For (+) strand synthesis using a (-) strand DNA template linked to the tRNA primer, only the first 18 nt of tRNA are replicated to re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burnett BP,McHenry CS

更新日期：1997-07-08 00:00:00

abstract：:The histidine periplasmic permease of Salmonella typhimurium has been partially purified and reconstituted into proteoliposomes. In this in vitro preparation, transport activity is completely dependent on the presence of all four permease proteins (HisJ, HisQ, HisM, and HisP) and on internal ATP. The reconstituted sys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bishop L,Agbayani R Jr,Ambudkar SV,Maloney PC,Ames GF

更新日期：1989-09-01 00:00:00

abstract：:The role of anti-peripheral nerve myelin antibody (anti-PNM Ab) in the pathogenesis of acquired demyelination of peripheral nerve is unclear, in part, due to the poor correlation between antibody and disease activity. Previous studies show that only 27-50% of patients with acute demyelinating neuropathy or Guillain-Ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koski CL,Humphrey R,Shin ML

更新日期：1985-02-01 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) signals through a receptor complex containing the type I (TGF-beta RI) and type II (TGF-beta RII) receptors. We describe here biochemical studies on early events in the TGF-beta signaling pathways. TGF-beta RII is highly phosphorylated when expressed alone in COS-1 cells; its...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen F,Weinberg RA

更新日期：1995-02-28 00:00:00

abstract：:In bacteria, the first two steps of gene expression-transcription and translation-are spatially and temporally coupled. Uncoupling may lead to the arrest of transcription through RNA polymerase backtracking, which interferes with replication forks, leading to DNA double-stranded breaks and genomic instability. How tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevenson-Jones F,Woodgate J,Castro-Roa D,Zenkin N

更新日期：2020-04-14 00:00:00

abstract：:Synthetic bradykinin, a nonapeptide formed from alpha-2 globulin in plasma, injected intra-arterially or intraperitoneally in cats in doses of 10-50 mug, evoked activity in the central nervous system in pathways associated with the signaling of pain. Similar injections of bradykinin in intact normal cats and dogs evok...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lim RK,Krauthamer G,Guzman F,Fulp RR

更新日期：1969-07-01 00:00:00

abstract：:We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Essevaz-Roulet B,Bockelmann U,Heslot F

更新日期：1997-10-28 00:00:00

abstract：:Although adaptor ADAP (FYB) and its binding to SLP-76 has been implicated in TcR-induced "inside-out" signaling for LFA-1 activation in T cells, little is known regarding its role in LFA-1-mediated "outside-in" signaling. In this study, we demonstrate that ADAP and SLP-76-ADAP binding are coupled to LFA-1 costimulatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Wei B,Bismuth G,Rudd CE

更新日期：2009-07-28 00:00:00

abstract：:Both mammals and birds use the interaural time difference (ITD) for localization of sound in the horizontal plane. They may localize either real or phantom sound sources, when the signal consists of a narrow frequency band. This ambiguity does not occur with broadband signals. A plot of impulse rates or amplitude of e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peña JL,Konishi M

更新日期：2000-10-24 00:00:00

abstract：:A key component of scientific communication is sufficient information for other researchers in the field to reproduce published findings. For computational and data-enabled research, this has often been interpreted to mean making available the raw data from which results were generated, the computer code that generate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stodden V,Seiler J,Ma Z

更新日期：2018-03-13 00:00:00

abstract：:Myosin generates force by a rotation of its lever arm. Crystal structures of myosin II indicate an unloaded working stroke of 10-12 nm, a range confirmed by recent x-ray interference experiments. However, when an actin filament, held between two weakly, optically trapped beads is made to interact with a single head of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sleep J,Lewalle A,Smith D

更新日期：2006-01-31 00:00:00

abstract：:Human T lymphotropic virus type 1 (HTLV-1) -associated myelopathy/tropic spastic paraparesis is a demyelinating inflammatory neurologic disease associated with HTLV-1 infection. HTLV-1 Tax11-19-specific cytotoxic T cells have been isolated from HLA-A2-positive patients. We have used a peptide-loaded soluble HLA-A2-Ig ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greten TF,Slansky JE,Kubota R,Soldan SS,Jaffee EM,Leist TP,Pardoll DM,Jacobson S,Schneck JP

更新日期：1998-06-23 00:00:00

abstract：:"Functional connectivity" techniques are commonplace tools for studying brain organization. A critical element of these analyses is to distinguish variance due to neurobiological signals from variance due to nonneurobiological signals. Multiecho fMRI techniques are a promising means for making such distinctions based ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Power JD,Plitt M,Gotts SJ,Kundu P,Voon V,Bandettini PA,Martin A

更新日期：2018-02-27 00:00:00

abstract：:Immune responses can interfere with the effective use of therapeutic proteins to treat genetic deficiencies and have been challenging to manage. To address this problem, we adapted and studied methods of immune tolerance used in canine organ transplantation research to soluble protein therapeutics. A tolerization regi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kakkis E,Lester T,Yang R,Tanaka C,Anand V,Lemontt J,Peinovich M,Passage M

更新日期：2004-01-20 00:00:00

abstract：:The 8;21 translocation is one of the most common specific rearrangements in acute myelogenous leukemia. We have identified markers (D21S65 and a Not I boundary clone, Not-42, referred to as probe B) flanking the chromosome 21 translocation breakpoint (21q22.3) that demonstrate physical linkage in normal genomic DNA, b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao J,Erickson P,Gardiner K,Le Beau MM,Diaz MO,Patterson D,Rowley JD,Drabkin HA

更新日期：1991-06-01 00:00:00

abstract：:Although sexual selection theory has proved successful in explaining a wide array of male ornaments, the function of ornaments occurring in females is largely unknown. Traditionally, female ornaments have been considered nonfunctional, being merely a genetically correlated response to selection for male ornamentation....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amundsen T,Forsgren E

更新日期：2001-11-06 00:00:00

abstract：:The suppressor of Hairy-wing [su(Hw)] protein exerts a polar effect on gene expression by repressing the function of transcriptional enhancers located distally from the promoter with respect to the location of su(Hw) binding sequences. The directionality of this effect suggests that the su(Hw) protein specifically int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Georgiev PG,Corces VG

更新日期：1995-05-23 00:00:00

abstract：:GB virus B (GBV-B), which infects tamarins, is the virus most closely related to hepatitis C virus (HCV). HCV has a protein (p7) that is believed to form an ion channel. It is critical for viability. In vitro studies suggest that GBV-B has an analogous but larger protein (p13). We found that substitutions of the -1 an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takikawa S,Engle RE,Emerson SU,Purcell RH,St Claire M,Bukh J

更新日期：2006-02-28 00:00:00

abstract：:Cytochrome c peroxidase reacts with peroxide to form compound I, which contains an oxyferryl heme and an indolyl radical at Trp-191. The indolyl free radical has a half-life of several hours at room temperature, and this remarkable stability is essential for the catalytic function of cytochrome c peroxidase. To probe ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller MA,Han GW,Kraut J

更新日期：1994-11-08 00:00:00

abstract：:Previous studies imply that the intracellular domain of Notch1 must translocate to the nucleus for its activity. In this study, we demonstrate that a mNotch1 mutant protein that lacks its extracellular domain but retains its membrane-spanning region becomes proteolytically processed on its intracellular surface and, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kopan R,Schroeter EH,Weintraub H,Nye JS

更新日期：1996-02-20 00:00:00

abstract：:Signal transduction pathways activated by chemoattractants have been extensively studied, but little is known about the events mediating responses to mechanical stimuli. We discovered that acute mechanical perturbation of cells triggered transient activation of all tested components of the chemotactic signal transduct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Artemenko Y,Axiotakis L Jr,Borleis J,Iglesias PA,Devreotes PN

更新日期：2016-11-22 00:00:00

abstract：:Cancer immunotherapy has emerged as a promising therapeutic intervention. However, complete and durable responses are only seen in a fraction of patients who have cancer. A key factor that limits therapeutic success is the infiltration of tumors by cells of the myeloid lineage. The inhibitory receptor signal regulator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ring NG,Herndler-Brandstetter D,Weiskopf K,Shan L,Volkmer JP,George BM,Lietzenmayer M,McKenna KM,Naik TJ,McCarty A,Zheng Y,Ring AM,Flavell RA,Weissman IL

更新日期：2017-12-05 00:00:00

abstract：:Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here, we present a proof-of-concept study to dete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calligaris D,Caragacianu D,Liu X,Norton I,Thompson CJ,Richardson AL,Golshan M,Easterling ML,Santagata S,Dillon DA,Jolesz FA,Agar NY

更新日期：2014-10-21 00:00:00

abstract：:Despite nearly four decades of effort, broad inhibition of oncogenic RAS using small-molecule approaches has proven to be a major challenge. Here we describe the development of a pan-RAS biologic inhibitor composed of the RAS-RAP1-specific endopeptidase fused to the protein delivery machinery of diphtheria toxin. We s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vidimar V,Beilhartz GL,Park M,Biancucci M,Kieffer MB,Gius DR,Melnyk RA,Satchell KJF

更新日期：2020-07-21 00:00:00

abstract：:Impaired regulatory T-cell function results in a severe chronic autoimmune disease affecting multiple organs in Scurfy mice and humans with the immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome. Previous studies have shown that T helper cells but not cytotoxic T cells are critical for the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aschermann S,Lehmann CH,Mihai S,Schett G,Dudziak D,Nimmerjahn F

更新日期：2013-11-19 00:00:00

abstract：:Cardiomyopathy (CM) is a primary degenerative disease of myocardium and is traditionally categorized into hypertrophic and dilated CMs (HCM and DCM) according to its gross appearance. Cardiomyopathic hamster (CM hamster), a representative model of human hereditary CM, has HCM and DCM inbred sublines, both of which des...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakamoto A,Ono K,Abe M,Jasmin G,Eki T,Murakami Y,Masaki T,Toyo-oka T,Hanaoka F

更新日期：1997-12-09 00:00:00