Abstract:
:The present study aimed to explore the possible radioprotective effects of celastrol and relevant molecular mechanisms in an in vitro cell and in vivo mouse models exposed to gamma radiation. Human keratinocytes (HaCaT) and foreskin fibroblast (BJ) cells were exposed to gamma radiation of 20Gy, followed by treatment with celastrol for 24 h. Cell viability, reactive oxygen species (ROS), nitric oxide (NO) and glutathione (GSH) production, lipid peroxidation, DNA damage, inflammatory cytokine levels, and NF-κB pathway activation were examined. The survival rate, levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in blood, and p65 and phospho-p65 expression were also evaluated in mice after exposure to gamma radiation and celastrol treatment. The gamma irradiation of HaCaT cells induced decreased cell viability, but treatment with celastrol significantly blocked this cytotoxicity. Gamma irradiation also increased free radical production (e.g., ROS and NO), decreased the level of GSH, and enhanced oxidative DNA damage and lipid peroxidation in cells, which were effectively reversed by celastrol treatment. Moreover, inflammatory responses induced by gamma irradiation, as demonstrated by increased levels of IL-6, TNF-α, and IL-1β, were also blocked by celastrol. The increased activity of NF-κB DNA binding following gamma radiation was significantly attenuated after celastrol treatment. In the irradiated mice, treatment with celastrol significantly improved overall survival rate, reduced the excessive inflammatory responses, and decreased NF-κB activity. As a NF-κB pathway blocker and antioxidant, celastrol may represent a promising pharmacological agent with protective effects against gamma irradiation-induced injury.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Wang H,Ahn KS,Alharbi SA,Shair OH,Arfuso F,Sethi G,Chinnathambi A,Tang FRdoi
10.3390/ijms21031084subject
Has Abstractpub_date
2020-02-06 00:00:00issue
3issn
1422-0067pii
ijms21031084journal_volume
21pub_type
杂志文章abstract::As the majority of experimental studies suggest cadmium being metalloestrogen, we examined cadmium/breast cancer (BC) association by histological and tumor receptor subtype in 509 invasive BC patients and 1170 controls. Urinary cadmium was determined by atomic absorption spectrometry, and categorized using tertiles of...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123029
更新日期:2019-06-21 00:00:00
abstract::Systemic sclerosis is a rare chronic heterogenous disease that involves inflammation and vasculopathy, and converges in end-stage development of multisystem tissue fibrosis. The loss of tight spatial distribution and temporal expression of proteins in the extracellular matrix (ECM) leads to progressive organ stiffenin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21134776
更新日期:2020-07-06 00:00:00
abstract::Today, cancer is understood as an epigenetic as well as genetic disease. The main epigenetic hallmarks of the cancer cell are DNA methylation and histone modifications. Proteins such as histone deacetylases (HDACs) that cause modifications of histones and other proteins can be targets for novel anticancer agents. Rece...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160818252
更新日期:2015-08-06 00:00:00
abstract::A simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of Radix Isatidis (dried root of Isatis indigotica) for chemical fingerprint analysis and quantitative analysis of eight bioactive constituents, including R,S-goitrin, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13079035
更新日期:2012-01-01 00:00:00
abstract::RNA virus genomes are multifunctional entities endowed with conserved structural elements that control translation, replication and encapsidation, among other processes. The preservation of these structural RNA elements constraints the genomic sequence variability. The hepatitis C virus (HCV) genome is a positive, sin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21041479
更新日期:2020-02-21 00:00:00
abstract::Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms130810410
更新日期:2012-01-01 00:00:00
abstract::Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of Glycine sojaAAE3 (GsAAE3) in Cadmium (Cd) and Aluminum (Al) tolerances. The recomb...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21228869
更新日期:2020-11-23 00:00:00
abstract::Transient Receptor Potential (TRP) channels are a family of ion channels whose members are distributed among all kinds of animals, from invertebrates to vertebrates. The importance of these molecules is exemplified by the variety of physiological roles they play. Perhaps, the most extensively studied member of this fa...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21113837
更新日期:2020-05-28 00:00:00
abstract::Agricultural production continues to be constrained by a number of biotic and abiotic factors that can reduce crop yield quantity and quality. Potassium (K) is an essential nutrient that affects most of the biochemical and physiological processes that influence plant growth and metabolism. It also contributes to the s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms14047370
更新日期:2013-04-02 00:00:00
abstract::The selective binding of six (S)-quinuclidine-triazoles and their (R)-enantiomers to nicotinic acetylcholine receptor (nAChR) subtypes α3β4 and α7, respectively, were analyzed by in silico docking to provide the insight into the molecular basis for the observed stereospecific subtype discrimination. Homology modeling ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176189
更新日期:2020-08-27 00:00:00
abstract::Molecular imprinted polymers (MIP) are considered one of the most promising selective and novel separation methods for removal phenolic compound in wastewater treatment. MIP are crosslinked polymeric materials that exhibit high binding capacity and selectivity towards a target molecule (template), purposely present du...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10010354
更新日期:2009-01-01 00:00:00
abstract::The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) indu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215367
更新日期:2019-10-28 00:00:00
abstract::The DNA excision repair protein ERCC-1-DNA repair endonuclease XPF (ERCC1-XPF) is a heterodimeric endonuclease essential for the nucleotide excision repair (NER) DNA repair pathway. Although its activity is required to maintain genome integrity in healthy cells, ERCC1-XPF can counteract the effect of DNA-damaging ther...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19051328
更新日期:2018-04-30 00:00:00
abstract::Streptococcus pneumoniae is a major meningitis-causing pathogen globally, bringing about significant morbidity and mortality, as well as long-term neurological sequelae in almost half of the survivors. Subsequent to nasopharyngeal colonisation and systemic invasion, translocation across the blood‒brain barrier (BBB) b...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19113555
更新日期:2018-11-11 00:00:00
abstract::Tumours are not merely masses of abnormally proliferating cancer cells[...]. ...
journal_title:International journal of molecular sciences
pub_type: 社论
doi:10.3390/ijms19030843
更新日期:2018-03-14 00:00:00
abstract::Osteoarthritis (OA) is the most common joint disease that causes pain and disability in the adult population. OA is primarily caused by trauma induced by an external force or by age-related cartilage damage. Chondrocyte hypertrophy or chondrocyte senescence is thought to play a role in the initiation and progression o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21072358
更新日期:2020-03-29 00:00:00
abstract::Calorie restriction (CR) inhibits prostate cancer progression, partially through modulation of the IGF axis. IGF-1 receptor (IGF-1R) blockade reduces prostate cancer xenograft growth. We hypothesized that combining calorie restriction with IGF-1R blockade would have an additive effect on prostate cancer growth. Severe...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms140713782
更新日期:2013-07-03 00:00:00
abstract::Microbes have co-evolved with human beings for millions of years. They play a very important role in maintaining the health of the host. With the advancement in next generation sequencing technology, the microbiome profiling in the host can be obtained under different circumstances. This review focuses on the current ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms161125957
更新日期:2015-11-03 00:00:00
abstract::The intriguingly complex glomerular podocyte has been a recent object of intense study. Researchers have sought to understand its role in the pathogenesis of common proteinuric diseases such as minimal change disease and focal segmental glomerular sclerosis. In particular, considerable effort has been directed towards...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms151121366
更新日期:2014-11-18 00:00:00
abstract::The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), pre...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082869
更新日期:2020-04-20 00:00:00
abstract::Nanosilver plays an important role in nanoscience and nanotechnology, and is becoming increasingly used for applications in nanomedicine. Nanosilver ranges from 1 to 100 nanometers in diameter. Smaller particles more readily enter cells and interact with the cellular components. The exposure dose, particle size, coati...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19072030
更新日期:2018-07-12 00:00:00
abstract::Transfer RNAs (tRNAs) are key players of protein synthesis, as they decode the genetic information organized in mRNA codons, translating them into the code of 20 amino acids. To be fully active, tRNAs undergo extensive post-transcriptional modifications, catalyzed by different tRNA-modifying enzymes. Lack of these mod...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123738
更新日期:2018-11-24 00:00:00
abstract::Platelet P2Y12 is an important adenosine diphosphate (ADP) receptor that is involved in agonist-induced platelet aggregation and is a valuable target for the development of anti-platelet drugs. Here we characterise the effects of thio analogues of uridine triphosphate (UTP) on ADP-induced platelet aggregation. Using h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020269
更新日期:2017-01-29 00:00:00
abstract::Indisputably, cancer is a global crisis that requires immediate intervention. Despite the use of conventional treatments over the past decades, it is acceptable to admit that these are expensive, invasive, associated with many side effects and, therefore, a reduced quality of life. One of the most possible solutions t...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20194771
更新日期:2019-09-26 00:00:00
abstract::Neonatal seizures are one of the most common comorbidities of neonatal encephalopathy, with seizures aggravating acute injury and clinical outcomes. Current treatment can control early life seizures; however, a high level of pharmacoresistance remains among infants, with increasing evidence suggesting current anti-sei...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21217832
更新日期:2020-10-22 00:00:00
abstract::C-type lectin receptors and their adaptor molecules are involved in the recognition of glycosylated self-antigens and pathogens. However, little is known about the species- and organ-specific expression profiles of these molecules. We therefore determined the mRNA expression levels of Dectin-1, MR1, MR2, DC-SIGN, Syk,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms130810113
更新日期:2012-01-01 00:00:00
abstract::Pigeon pea (Cajanus cajan (L.) Millspaugh) is cultivated widely in semiarid agricultural regions in over 90 countries around the world. This important legume can enter into symbiotic associations with a wide range of rhizobia including Bradyrhizobium and fast-growing rhizobia. In comparison with other major legumes su...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20051091
更新日期:2019-03-03 00:00:00
abstract:BACKGROUND:Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20143536
更新日期:2019-07-19 00:00:00
abstract::EAAC1 is important in modulating brain ischemic tolerance. Mice lacking EAAC1 exhibit increased susceptibility to neuronal oxidative stress in mice after transient cerebral ischemia. EAAC1 was first described as a glutamate transporter but later recognized to also function as a cysteine transporter in neurons. EAAC1-m...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151119444
更新日期:2014-10-27 00:00:00
abstract::Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development and programs the offspring's health for developing cardiovascular and metabolic disease later in life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides ...
journal_title:International journal of molecular sciences
pub_type: 临床试验,杂志文章
doi:10.3390/ijms21030834
更新日期:2020-01-28 00:00:00