Celastrol Alleviates Gamma Irradiation-Induced Damage by Modulating Diverse Inflammatory Mediators.


:The present study aimed to explore the possible radioprotective effects of celastrol and relevant molecular mechanisms in an in vitro cell and in vivo mouse models exposed to gamma radiation. Human keratinocytes (HaCaT) and foreskin fibroblast (BJ) cells were exposed to gamma radiation of 20Gy, followed by treatment with celastrol for 24 h. Cell viability, reactive oxygen species (ROS), nitric oxide (NO) and glutathione (GSH) production, lipid peroxidation, DNA damage, inflammatory cytokine levels, and NF-κB pathway activation were examined. The survival rate, levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in blood, and p65 and phospho-p65 expression were also evaluated in mice after exposure to gamma radiation and celastrol treatment. The gamma irradiation of HaCaT cells induced decreased cell viability, but treatment with celastrol significantly blocked this cytotoxicity. Gamma irradiation also increased free radical production (e.g., ROS and NO), decreased the level of GSH, and enhanced oxidative DNA damage and lipid peroxidation in cells, which were effectively reversed by celastrol treatment. Moreover, inflammatory responses induced by gamma irradiation, as demonstrated by increased levels of IL-6, TNF-α, and IL-1β, were also blocked by celastrol. The increased activity of NF-κB DNA binding following gamma radiation was significantly attenuated after celastrol treatment. In the irradiated mice, treatment with celastrol significantly improved overall survival rate, reduced the excessive inflammatory responses, and decreased NF-κB activity. As a NF-κB pathway blocker and antioxidant, celastrol may represent a promising pharmacological agent with protective effects against gamma irradiation-induced injury.


Int J Mol Sci


Wang H,Ahn KS,Alharbi SA,Shair OH,Arfuso F,Sethi G,Chinnathambi A,Tang FR




Has Abstract


2020-02-06 00:00:00










  • Cadmium Exposure and Risk of Breast Cancer by Histological and Tumor Receptor Subtype in White Caucasian Women: A Hospital-Based Case-Control Study.

    abstract::As the majority of experimental studies suggest cadmium being metalloestrogen, we examined cadmium/breast cancer (BC) association by histological and tumor receptor subtype in 509 invasive BC patients and 1170 controls. Urinary cadmium was determined by atomic absorption spectrometry, and categorized using tertiles of...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Strumylaite L,Kregzdyte R,Bogusevicius A,Poskiene L,Baranauskiene D,Pranys D

    更新日期:2019-06-21 00:00:00

  • Emerging Roles of Matricellular Proteins in Systemic Sclerosis.

    abstract::Systemic sclerosis is a rare chronic heterogenous disease that involves inflammation and vasculopathy, and converges in end-stage development of multisystem tissue fibrosis. The loss of tight spatial distribution and temporal expression of proteins in the extracellular matrix (ECM) leads to progressive organ stiffenin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Feng D,Gerarduzzi C

    更新日期:2020-07-06 00:00:00

  • The Histone Deacetylase Inhibitor BML-210 Influences Gene and Protein Expression in Human Promyelocytic Leukemia NB4 Cells via Epigenetic Reprogramming.

    abstract::Today, cancer is understood as an epigenetic as well as genetic disease. The main epigenetic hallmarks of the cancer cell are DNA methylation and histone modifications. Proteins such as histone deacetylases (HDACs) that cause modifications of histones and other proteins can be targets for novel anticancer agents. Rece...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Borutinskaitė V,Navakauskienė R

    更新日期:2015-08-06 00:00:00

  • Quantitative and chemical fingerprint analysis for the quality evaluation of Isatis indigotica based on ultra-performance liquid chromatography with photodiode array detector combined with chemometric methods.

    abstract::A simple and reliable method of ultra-performance liquid chromatography with photodiode array detector (UPLC-PDA) was developed to control the quality of Radix Isatidis (dried root of Isatis indigotica) for chemical fingerprint analysis and quantitative analysis of eight bioactive constituents, including R,S-goitrin, ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Shi YH,Xie ZY,Wang R,Huang SJ,Li YM,Wang ZT

    更新日期:2012-01-01 00:00:00

  • The Role of the RNA-RNA Interactome in the Hepatitis C Virus Life Cycle.

    abstract::RNA virus genomes are multifunctional entities endowed with conserved structural elements that control translation, replication and encapsidation, among other processes. The preservation of these structural RNA elements constraints the genomic sequence variability. The hepatitis C virus (HCV) genome is a positive, sin...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Romero-López C,Berzal-Herranz A

    更新日期:2020-02-21 00:00:00

  • Identification of tillering node proteins differentially accumulated in barley recombinant inbred lines with different juvenile growth habits.

    abstract::Barley (Hordeum vulgare L.) is an important cereal crop grown for both the feed and malting industries. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in th...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kuczyńska A,Kosmala A,Surma M,Adamski T

    更新日期:2012-01-01 00:00:00

  • Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses.

    abstract::Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of Glycine sojaAAE3 (GsAAE3) in Cadmium (Cd) and Aluminum (Al) tolerances. The recomb...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Xian P,Cai Z,Cheng Y,Lin R,Lian T,Ma Q,Nian H

    更新日期:2020-11-23 00:00:00

  • TRPV4: A Physio and Pathophysiologically Significant Ion Channel.

    abstract::Transient Receptor Potential (TRP) channels are a family of ion channels whose members are distributed among all kinds of animals, from invertebrates to vertebrates. The importance of these molecules is exemplified by the variety of physiological roles they play. Perhaps, the most extensively studied member of this fa...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Rosenbaum T,Benítez-Angeles M,Sánchez-Hernández R,Morales-Lázaro SL,Hiriart M,Morales-Buenrostro LE,Torres-Quiroz F

    更新日期:2020-05-28 00:00:00

  • The critical role of potassium in plant stress response.

    abstract::Agricultural production continues to be constrained by a number of biotic and abiotic factors that can reduce crop yield quantity and quality. Potassium (K) is an essential nutrient that affects most of the biochemical and physiological processes that influence plant growth and metabolism. It also contributes to the s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Wang M,Zheng Q,Shen Q,Guo S

    更新日期:2013-04-02 00:00:00

  • In Silico Finding of Key Interaction Mediated α3β4 and α7 Nicotinic Acetylcholine Receptor Ligand Selectivity of Quinuclidine-Triazole Chemotype.

    abstract::The selective binding of six (S)-quinuclidine-triazoles and their (R)-enantiomers to nicotinic acetylcholine receptor (nAChR) subtypes α3β4 and α7, respectively, were analyzed by in silico docking to provide the insight into the molecular basis for the observed stereospecific subtype discrimination. Homology modeling ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Arunrungvichian K,Chongruchiroj S,Sarasamkan J,Schüürmann G,Brust P,Vajragupta O

    更新日期:2020-08-27 00:00:00

  • Synthesis and evaluation of a molecularly imprinted polymer for 2,4-dinitrophenol.

    abstract::Molecular imprinted polymers (MIP) are considered one of the most promising selective and novel separation methods for removal phenolic compound in wastewater treatment. MIP are crosslinked polymeric materials that exhibit high binding capacity and selectivity towards a target molecule (template), purposely present du...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zakaria ND,Yusof NA,Haron J,Abdullah AH

    更新日期:2009-01-01 00:00:00

  • Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice.

    abstract::The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) indu...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Madokoro Y,Yoshino Y,Kato D,Sato T,Mizuno M,Kanamori T,Shimazawa M,Hida H,Hara H,Yoshida M,Borlongan CV,Ojika K,Matsukawa N

    更新日期:2019-10-28 00:00:00

  • Computational Characterization of Small Molecules Binding to the Human XPF Active Site and Virtual Screening to Identify Potential New DNA Repair Inhibitors Targeting the ERCC1-XPF Endonuclease.

    abstract::The DNA excision repair protein ERCC-1-DNA repair endonuclease XPF (ERCC1-XPF) is a heterodimeric endonuclease essential for the nucleotide excision repair (NER) DNA repair pathway. Although its activity is required to maintain genome integrity in healthy cells, ERCC1-XPF can counteract the effect of DNA-damaging ther...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gentile F,Barakat KH,Tuszynski JA

    更新日期:2018-04-30 00:00:00

  • Blood‒Brain Barrier Pathology and CNS Outcomes in Streptococcus pneumoniae Meningitis.

    abstract::Streptococcus pneumoniae is a major meningitis-causing pathogen globally, bringing about significant morbidity and mortality, as well as long-term neurological sequelae in almost half of the survivors. Subsequent to nasopharyngeal colonisation and systemic invasion, translocation across the blood‒brain barrier (BBB) b...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Yau B,Hunt NH,Mitchell AJ,Too LK

    更新日期:2018-11-11 00:00:00

  • Chemical and Molecular Approach to Tumor Metastases.

    abstract::Tumours are not merely masses of abnormally proliferating cancer cells[...]. ...

    journal_title:International journal of molecular sciences

    pub_type: 社论


    authors: Bergamo A,Sava G

    更新日期:2018-03-14 00:00:00

  • The Role of Chondrocyte Hypertrophy and Senescence in Osteoarthritis Initiation and Progression.

    abstract::Osteoarthritis (OA) is the most common joint disease that causes pain and disability in the adult population. OA is primarily caused by trauma induced by an external force or by age-related cartilage damage. Chondrocyte hypertrophy or chondrocyte senescence is thought to play a role in the initiation and progression o...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Rim YA,Nam Y,Ju JH

    更新日期:2020-03-29 00:00:00

  • Effects of calorie restriction and IGF-1 receptor blockade on the progression of 22Rv1 prostate cancer xenografts.

    abstract::Calorie restriction (CR) inhibits prostate cancer progression, partially through modulation of the IGF axis. IGF-1 receptor (IGF-1R) blockade reduces prostate cancer xenograft growth. We hypothesized that combining calorie restriction with IGF-1R blockade would have an additive effect on prostate cancer growth. Severe...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Galet C,Gray A,Said JW,Castor B,Wan J,Beltran PJ,Calzone FJ,Elashoff D,Cohen P,Aronson WJ

    更新日期:2013-07-03 00:00:00

  • Metagenomics: A New Way to Illustrate the Crosstalk between Infectious Diseases and Host Microbiome.

    abstract::Microbes have co-evolved with human beings for millions of years. They play a very important role in maintaining the health of the host. With the advancement in next generation sequencing technology, the microbiome profiling in the host can be obtained under different circumstances. This review focuses on the current ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zhang Y,Lun CY,Tsui SK

    更新日期:2015-11-03 00:00:00

  • Small molecule membrane transporters in the mammalian podocyte: a pathogenic and therapeutic target.

    abstract::The intriguingly complex glomerular podocyte has been a recent object of intense study. Researchers have sought to understand its role in the pathogenesis of common proteinuric diseases such as minimal change disease and focal segmental glomerular sclerosis. In particular, considerable effort has been directed towards...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zennaro C,Artero M,Di Maso V,Carraro M

    更新日期:2014-11-18 00:00:00

  • The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer.

    abstract::The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), pre...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Grambow F,Rutkowski R,Podmelle F,Schmoeckel K,Siegerist F,Domanski G,Schuster MW,Domanska G

    更新日期:2020-04-20 00:00:00

  • A Current Overview of the Biological and Cellular Effects of Nanosilver.

    abstract::Nanosilver plays an important role in nanoscience and nanotechnology, and is becoming increasingly used for applications in nanomedicine. Nanosilver ranges from 1 to 100 nanometers in diameter. Smaller particles more readily enter cells and interact with the cellular components. The exposure dose, particle size, coati...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Cameron SJ,Hosseinian F,Willmore WG

    更新日期:2018-07-12 00:00:00

  • Impact of tRNA Modifications and tRNA-Modifying Enzymes on Proteostasis and Human Disease.

    abstract::Transfer RNAs (tRNAs) are key players of protein synthesis, as they decode the genetic information organized in mRNA codons, translating them into the code of 20 amino acids. To be fully active, tRNAs undergo extensive post-transcriptional modifications, catalyzed by different tRNA-modifying enzymes. Lack of these mod...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Pereira M,Francisco S,Varanda AS,Santos M,Santos MAS,Soares AR

    更新日期:2018-11-24 00:00:00

  • Uridine Triphosphate Thio Analogues Inhibit Platelet P2Y12 Receptor and Aggregation.

    abstract::Platelet P2Y12 is an important adenosine diphosphate (ADP) receptor that is involved in agonist-induced platelet aggregation and is a valuable target for the development of anti-platelet drugs. Here we characterise the effects of thio analogues of uridine triphosphate (UTP) on ADP-induced platelet aggregation. Using h...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Gündüz D,Tanislav C,Sedding D,Parahuleva M,Santoso S,Troidl C,Hamm CW,Aslam M

    更新日期:2017-01-29 00:00:00

  • Enhancing Breast Cancer Treatment Using a Combination of Cannabidiol and Gold Nanoparticles for Photodynamic Therapy.

    abstract::Indisputably, cancer is a global crisis that requires immediate intervention. Despite the use of conventional treatments over the past decades, it is acceptable to admit that these are expensive, invasive, associated with many side effects and, therefore, a reduced quality of life. One of the most possible solutions t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: R Mokoena D,P George B,Abrahamse H

    更新日期:2019-09-26 00:00:00

  • Neonatal Seizures and Purinergic Signalling.

    abstract::Neonatal seizures are one of the most common comorbidities of neonatal encephalopathy, with seizures aggravating acute injury and clinical outcomes. Current treatment can control early life seizures; however, a high level of pharmacoresistance remains among infants, with increasing evidence suggesting current anti-sei...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Menéndez Méndez A,Smith J,Engel T

    更新日期:2020-10-22 00:00:00

  • Quantitative expression of C-type lectin receptors in humans and mice.

    abstract::C-type lectin receptors and their adaptor molecules are involved in the recognition of glycosylated self-antigens and pathogens. However, little is known about the species- and organ-specific expression profiles of these molecules. We therefore determined the mRNA expression levels of Dectin-1, MR1, MR2, DC-SIGN, Syk,...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lech M,Susanti HE,Römmele C,Gröbmayr R,Günthner R,Anders HJ

    更新日期:2012-01-01 00:00:00

  • Classical Soybean (Glycine max (L.) Merr) Symbionts, Sinorhizobium fredii USDA191 and Bradyrhizobium diazoefficiens USDA110, Reveal Contrasting Symbiotic Phenotype on Pigeon Pea (Cajanus cajan (L.) Millsp).

    abstract::Pigeon pea (Cajanus cajan (L.) Millspaugh) is cultivated widely in semiarid agricultural regions in over 90 countries around the world. This important legume can enter into symbiotic associations with a wide range of rhizobia including Bradyrhizobium and fast-growing rhizobia. In comparison with other major legumes su...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Alaswad AA,Oehrle NW,Krishnan HB

    更新日期:2019-03-03 00:00:00

  • The Effect of Uncoated SPIONs on hiPSC-Differentiated Endothelial Cells.

    abstract:BACKGROUND:Endothelial progenitor cells (EPCs) were indicated in vascular repair, angiogenesis of ischemic organs, and inhibition of formation of initial hyperplasia. Differentiation of endothelial cells (ECs) from human induced pluripotent stem cells (hiPSC)-derived endothelial cells (hiPSC-ECs) provides an unlimited ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Salingova B,Simara P,Matula P,Zajickova L,Synek P,Jasek O,Veverkova L,Sedlackova M,Nichtova Z,Koutna I

    更新日期:2019-07-19 00:00:00

  • EAAC1 gene deletion increases neuronal death and blood brain barrier disruption after transient cerebral ischemia in female mice.

    abstract::EAAC1 is important in modulating brain ischemic tolerance. Mice lacking EAAC1 exhibit increased susceptibility to neuronal oxidative stress in mice after transient cerebral ischemia. EAAC1 was first described as a glutamate transporter but later recognized to also function as a cysteine transporter in neurons. EAAC1-m...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Choi BY,Kim JH,Kim HJ,Lee BE,Kim IY,Sohn M,Suh SW

    更新日期:2014-10-27 00:00:00

  • Maternal Overweight Downregulates MME (Neprilysin) in Feto-Placental Endothelial Cells and in Cord Blood.

    abstract::Maternal overweight in pregnancy alters the metabolic environment and generates chronic low-grade inflammation. This affects fetal development and programs the offspring's health for developing cardiovascular and metabolic disease later in life. MME (membrane-metalloendopeptidase, neprilysin) cleaves various peptides ...

    journal_title:International journal of molecular sciences

    pub_type: 临床试验,杂志文章


    authors: Weiß E,Berger HM,Brandl WT,Strutz J,Hirschmugl B,Simovic V,Tam-Ammersdorfer C,Cvitic S,Hiden U

    更新日期:2020-01-28 00:00:00