Preparation and optimization of fisetin loaded glycerol based soft nanovesicles by Box-Behnken design.


:The present study focused on the development and optimization of glycerosomes for dermal delivery of fisetin. The fisetin loaded glycerosomes formulation was optimized by Box-Behnken design. The independent variables were the lipoid S 100, glycerol, and sonication time, whereas the dependent variables were the vesicles size, entrapment efficiency, and flux. The mechanism of skin penetration of fisetin loaded glycerosomes formulation was determined by the DSC and FTIR studies. Confocal scanning microscopy was used to detect the penetration ability of glycerosomes. The optimized fisetin loaded glycerosomes formulation was converted into a Carbopol® gel matrix, and the latter was analyzed for various parameters. The optimized formulation of glycerosomes presented vesicles size, entrapment efficiency and flux of 138.8 ± 4.09 nm, 86.41 ± 2.95% and 5.04 ± 0.17 µg/cm2/h, respectively. The transmission electron microscopy of optimized fisetin loaded formulation revealed the spherical and sealed structure of glycerosomes vesicles. The confocal study confirmed that the Rhodamine B incorporated glycerosomes penetrated up to deeper layers of skin. The DSC and FTIR studies revealed that the hydration of skin layers and skin lipid fluidization could be the penetration mechanism of fisetin glycerosomes formulation. The optimized fisetin loaded glycerosomes gel formulation presented a flux of 4.24 ± 0.14 μg/cm2/h, and exhibited zero-order release kinetics. The texture analysis of fisetin glycerosomes gel displayed a sufficient hardness, consistency, cohesiveness, and index of viscosity. It was concluded that the prepared fisetin loaded glycerosomes gel was suitable for the dermal application.


Int J Pharm


Moolakkadath T,Aqil M,Ahad A,Imam SS,Praveen A,Sultana Y,Mujeeb M




Has Abstract


2020-03-30 00:00:00












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    abstract::Isooctyl p-methoxycinnamate(OMC) is a commonly used chemical ultraviolet B sunscreen that suffers rapid degradation with current delivery systems following sun exposure. In this study, deoxycholate-mediated liposome (DOC-LS) endocytosis was employed to improve the antioxidation effects of OMC following topical adminis...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Zhang Y,Shen L,Zhang K,Guo T,Zhao J,Li N,Feng N

    更新日期:2015-12-30 00:00:00

  • Synthesis, metabolism and cellular permeability of enzymatically stable dipeptide prodrugs of acyclovir.

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    journal_title:International journal of pharmaceutics

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    authors: Talluri RS,Samanta SK,Gaudana R,Mitra AK

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  • Particle engineering of materials for oral inhalation by dry powder inhalers. II-Sodium cromoglicate.

    abstract::Sodium cromoglicate is an antiasthmatic and antiallergenic drug used in inhalation therapy and commonly administered by a dry powder inhaler. In the present study we sought to examine the feasibility of producing nanoporous microparticles (NPMPs) of this hydrophilic material by adaptation of a spray drying process pre...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Nolan LM,Li J,Tajber L,Corrigan OI,Healy AM

    更新日期:2011-02-28 00:00:00

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Trasi NS,Taylor LS

    更新日期:2015-12-30 00:00:00

  • A tunable extruded 3D printing platform using thermo-sensitive pastes.

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    authors: Yang Y,Wang X,Lin X,Xie L,Ivone R,Shen J,Yang G

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    pub_type: 杂志文章


    authors: Salvador A,Sandgren KJ,Liang F,Thompson EA,Koup RA,Pedraz JL,Hernandez RM,Loré K,Igartua M

    更新日期:2015-12-30 00:00:00

  • Alkyl glucopyranoside-based niosomes containing methotrexate for pharmaceutical applications: evaluation of physico-chemical and biological properties.

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    journal_title:International journal of pharmaceutics

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    authors: Muzzalupo R,Tavano L,La Mesa C

    更新日期:2013-12-15 00:00:00

  • Integrity and stability of oral liposomes containing bile salts studied in simulated and ex vivo gastrointestinal media.

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    authors: Hu S,Niu M,Hu F,Lu Y,Qi J,Yin Z,Wu W

    更新日期:2013-01-30 00:00:00

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Ghosh I,Bose S,Vippagunta R,Harmon F

    更新日期:2011-05-16 00:00:00

  • Complexation of cationic-neutral block polyelectrolyte with insulin and in vitro release studies.

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    authors: Pippa N,Karayianni M,Pispas S,Demetzos C

    更新日期:2015-08-01 00:00:00

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    journal_title:International journal of pharmaceutics

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    authors: Dobrowolski A,Strob R,Nietfeld J,Pieloth D,Wiggers H,Thommes M

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    authors: Patel M,Sheng Y,Mandava NK,Pal D,Mitra AK

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  • Combination of microneedles and microemulsions to increase celecoxib topical delivery for potential application in chemoprevention of breast cancer.

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    pub_type: 杂志文章


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  • Interaction of cationic partial dendrimers with charged and neutral liposomes.

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Purohit G,Sakthivel T,Florence AT

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  • Plasma pharmacokinetics and distribution of ruxolitinib into skin following oral and topical administration in minipigs.

    abstract::This preclinical study compared plasma concentrations and distribution of ruxolitinib in the skin of Göttingen minipigs following twice a day oral (40 mg/kg) versus topical administration (1.5% w/w cream applied to 10% of body surface area). Following oral administration, the plasma area-under-the-curve was approximat...

    journal_title:International journal of pharmaceutics

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  • Strain-specific differences in behaviour among Lacticaseibacillus rhamnosus cell wall mutants during direct compression.

    abstract::The human body harbours a large variety of microbial communities. It is already well-known that these communities play an important role in human health. Therefore, microbial imbalances can be responsible for several health disorders by different mechanisms. In recent years, probiotic bacteria have been increasingly a...

    journal_title:International journal of pharmaceutics

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    authors: Byl E,Jokicevic K,Kiekens S,Lebeer S,Kiekens F

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  • Stability of lyophilized human growth hormone.

    abstract::To evaluate relationships between the extent of protein-excipient interactions, structural relaxation of an amorphous matrix, and the physico-chemical stability of a protein, human growth hormone (hGH) was lyophilized with sucrose and trehalose in a 1:2 weight ratio. The protein-excipient interactions were analyzed im...

    journal_title:International journal of pharmaceutics

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    authors: Salnikova MS,Middaugh CR,Rytting JH

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  • Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery.

    abstract::Non-viral gene delivery vectors studied in the gene therapy applications are often designed with the cationic nitrogen containing groups necessary for binding and cell release of nucleic acids. Disadvantage is a relatively high toxicity which restricts the in vivo use of such nanoparticles. Here we show, that the 3rd ...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Herma R,Wrobel D,Liegertová M,Müllerová M,Strašák T,Maly M,Semerádtová A,Štofik M,Appelhans D,Maly J

    更新日期:2019-05-01 00:00:00

  • Preparation and evaluation of agglomerated crystals by crystallo-co-agglomeration: an integrated approach of principal component analysis and Box-Behnken experimental design.

    abstract::Poor mechanical properties of crystalline drug particles require wet granulation technique for tablet production which is uneconomical, laborious, and tedious. The present investigation was aimed to improve flow and mechanical properties of racecadotril (RCD), a poorly water soluble antidiarrheal agent, by a crystallo...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Garala KC,Patel JM,Dhingani AP,Dharamsi AT

    更新日期:2013-08-16 00:00:00

  • New insights into the pore structure of poly(d,l-lactide-co-glycolide) microspheres.

    abstract::The objective of this work was to develop a fast and significant method for the determination of the intraparticulate pore size distribution of microspheres. Poly(lactide-co-glycolide) (PLGA) microspheres prepared with a solvent extraction/evaporation process were studied. From the envelope and the skeletal volume of ...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Vay K,Scheler S,Friess W

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  • Non-viral dried powders for respiratory gene delivery prepared by cationic and chitosan loaded liposomes.

    abstract::The aim of this work was to investigate lipid-based dried powders as transfection competent carriers capable of promoting the expression of therapeutic genes. The lipid-based vectors were prepared by combining different cationic lipids 1,2-dioleoyl-3-trimethylammoniumpropane chloride (DOTAP), 1,2-dioleoyl-sn-glycero-3...

    journal_title:International journal of pharmaceutics

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    authors: Colonna C,Conti B,Genta I,Alpar OH

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  • The quantification of small degrees of disorder in lactose using solution calorimetry.

    abstract::There is a realisation that small quantities of amorphous material can have a significant impact on the properties of crystalline solids. Consequently there is a growing interest in quantifying the amount of amorphous material that is present in "crystalline powders". Success has been reported when using isothermal mi...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Hogan SE,Buckton G

    更新日期:2000-10-10 00:00:00

  • Enhancing analgesic and anti-inflammatory effects of capsaicin when loaded into olive oil nanoemulsion: An in vivo study.

    abstract::Topical preparations of capsaicin, the major pungent ingredient of hot pepper, are being used for management of pain and inflammatory disorders. Purpose of this study was to use nanoemulsion as an effective topical drug carrier for in vivo delivery of capsaicin. An oil-in-water nanoemulsion containing capsaicin was pr...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Ghiasi Z,Esmaeli F,Aghajani M,Ghazi-Khansari M,Faramarzi MA,Amani A

    更新日期:2019-03-25 00:00:00

  • Effects of gefitinib treatment on cellular uptake of extracellular vesicles in EGFR-mutant non-small cell lung cancer cells.

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Takenaka T,Nakai S,Katayama M,Hirano M,Ueno N,Noguchi K,Takatani-Nakase T,Fujii I,Kobayashi SS,Nakase I

    更新日期:2019-12-15 00:00:00

  • Leaching of pectin from mixed films containing pectin, chitosan and HPMC intended for biphasic drug delivery.

    abstract::Mixed films containing pectin, chitosan and HPMC, prepared by solvent casting from 0.1 M HCl (pH 1.5) and 0.1 M acetic acid (pH 2.9) were evaluated for their morphological and leaching properties. Films cast at pH 1.5 were uniform with smooth surfaces while films cast at pH 2.9 showed particle aggregation and had roug...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Ofori-Kwakye K,Fell JT

    更新日期:2003-01-02 00:00:00

  • Controlled-release triple anti-inflammatory therapy based on novel gastroretentive sponges: characterization and magnetic resonance imaging in healthy volunteers.

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Tadros MI,Fahmy RH

    更新日期:2014-09-10 00:00:00

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    abstract::In the present study, the features of two new thiolated polymers--the so-called thiomers--were investigated. Mediated by a carbodiimide cysteamine was covalently attached to sodium carboxymethylcellulose (Na-CMC) and neutralised polycarbophil (Na-PCP). Depending on the weight-ratio polymer to cysteamine during the cou...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Kast CE,Bernkop-Schnürch A

    更新日期:2002-03-02 00:00:00

  • Cationic solid lipid nanoparticles enhance ocular hypotensive effect of melatonin in rabbit.

    abstract::The study was aimed at evaluating whether the ocular hypotensive effect of melatonin (MEL) was enhanced by its encapsulation in cationic solid lipid nanoparticles (cSLN), as well as at determining the tolerability of these formulations on the ocular surface. MEL was loaded in cSLN that had already been shown to be sui...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Leonardi A,Bucolo C,Drago F,Salomone S,Pignatello R

    更新日期:2015-01-15 00:00:00

  • Development of low dose micro-tablets by high shear wet granulation process.

    abstract::Low dose micro-tablets with acceptable quality attributes, specifically content uniformity (CU), would not only enhance the dose flexibility in the clinic, but also decrease excipient burden in pediatric population. Considering the CU challenges associated with directly compressed low dose micro-tablets, in this study...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Gupta S,Thool P,Meruva S,Li J,Patel J,Agrawal A,Karki S,Bowen W,Mitra B

    更新日期:2020-09-25 00:00:00

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    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Liu W,Hu M,Liu W,Xue C,Xu H,Yang X

    更新日期:2008-11-19 00:00:00